pycom03g07560

Metallothionein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000858
Physical Location & Seq
Reverse (-)
5990640 .. 5991286
647 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07560.3

Sequence Viewer

Length: 228 bp
ATGTCTTCCAGTTGTAGCTGTGGTAGTGACTGCAAGTGCGGCAGTGGCTGCAAATGTGGGATGCACGTTGACTTGGGTTACTCAGAGACCACTTCCATTGATATCATCATTGCTGGGGTTGCACCAATGAAGATGTCCTTTGAGGGGTCTGAGATGAACTATGGAGCAGAGAATGACTGCAAGTGTGGCTCAAACTGCAGCTGCAGCTCATGCGGCTGCAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

76

Amino Acids

7.66

Weight (kDa)

4.75

Isoelectric Point (pI)

62.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 213
AfiI CCNNNNNNNGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 18, 201, 207
AluI AGCT 3 cut(s) 18, 201, 207
Alw26I GTCTC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 48
ApeKI GCWGC 5 cut(s) 48, 198, 201, 204, 216
BbvI GCAGC 5 cut(s) 35, 188, 203, 210, 216
BcoDI GTCTC 1 cut(s) 80
BfmI CTRYAG 2 cut(s) 196, 202
BisI GCNGC 7 cut(s) 40, 49, 199, 202, 205, 214, 217
BlsI GCNGC 7 cut(s) 41, 50, 200, 203, 206, 215, 218
BmsI GCATC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 144
Bse1I ACTGG 1 cut(s) 9
Bse3DI GCAATG 1 cut(s) 108
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 144
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 2 cut(s) 96, 141
BseNI ACTGG 1 cut(s) 9
BseXI GCAGC 5 cut(s) 35, 188, 203, 210, 216
BseYI CCCAGC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 80
BspACI CCGC 2 cut(s) 39, 213
BspCNI CTCAG 2 cut(s) 95, 142
BspMAI CTGCAG 2 cut(s) 200, 206
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 1 cut(s) 9
BstAPI GCANNNNNTGC 2 cut(s) 48, 210
BstDEI CTNAG 2 cut(s) 82, 150
BstF5I GGATG 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 80
BstMWI GCNNNNNNNGC 9 cut(s) 39, 45, 48, 119, 186, 195, 204, 210, 213
BstSFI CTRYAG 2 cut(s) 196, 202
BstV1I GCAGC 5 cut(s) 35, 188, 203, 210, 216
BtsCI GGATG 1 cut(s) 66
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
CaiI CAGNNNCTG 1 cut(s) 48
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 1 cut(s) 210
CviJI RGCY 6 cut(s) 18, 48, 189, 201, 207, 216
CviKI_1 RGCY 6 cut(s) 18, 48, 189, 201, 207, 216
DdeI CTNAG 2 cut(s) 82, 150
Eco31I GGTCTC 1 cut(s) 80
Eco32I GATATC 1 cut(s) 103
EcoRV GATATC 1 cut(s) 103
FaeI CATG 1 cut(s) 213
FaiI YATR 2 cut(s) 162, 211
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FatI CATG 1 cut(s) 209
Fnu4HI GCNGC 7 cut(s) 40, 49, 199, 202, 205, 214, 217
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 40, 49, 199, 202, 205, 214, 217
GluI GCNGC 7 cut(s) 40, 49, 199, 202, 205, 214, 217
GsaI CCCAGC 1 cut(s) 117
Hin1II CATG 1 cut(s) 213
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 85, 151
Hpy8I GTNNAC 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 8 cut(s) 33, 51, 64, 122, 180, 198, 204, 219
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 45, 48, 119, 186, 195, 204, 210, 213
HpyF3I CTNAG 2 cut(s) 82, 150
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 1 cut(s) 213
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 2 cut(s) 22, 99
Lsp1109I GCAGC 5 cut(s) 35, 188, 203, 210, 216
LweI GCATC 1 cut(s) 51
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 2 cut(s) 26, 77
MboII GAAGA 1 cut(s) 142
MnlI CCTC 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 201
MwoI GCNNNNNNNGC 9 cut(s) 39, 45, 48, 119, 186, 195, 204, 210, 213
NlaIII CATG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 26
PkrI GCNGC 7 cut(s) 41, 50, 200, 203, 206, 215, 218
PspFI CCCAGC 1 cut(s) 113
PstI CTGCAG 2 cut(s) 200, 206
PstNI CAGNNNCTG 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 201
SatI GCNGC 7 cut(s) 40, 49, 199, 202, 205, 214, 217
SetI ASST 4 cut(s) 20, 69, 203, 209
SfaNI GCATC 1 cut(s) 51
SfcI CTRYAG 2 cut(s) 196, 202
SgeI CNNG 7 cut(s) 21, 46, 77, 85, 126, 193, 222
SsiI CCGC 2 cut(s) 39, 213
TaiI ACGT 1 cut(s) 69
TauI GCSGC 2 cut(s) 42, 216
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 5 cut(s) 48, 198, 201, 204, 216
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 2 cut(s) 143, 170
TspRI CASTG 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.