MD11G1109400.v1.1

Metallothionein-like protein type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000858
Physical Location & Seq
Reverse (-)
9670389 .. 9671450
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1109400.v1.1.491

Sequence Viewer

Length: 225 bp
ATGTCTAGCAATTGTAGCTGTGGTAGTGACTGCAAGTGCGGCAGTGGCTGCAAATGTGGGATGCACGCTGACTTGGGTTACTCAGAGACCACTTCCACTACCATCATTGCTGGGGTTGCACCAGTGAAGACGTTCTCTGAGGGGTCTGAGATGAACTATGGAGCAGAGAATGACTGCAAGTGTGGCTCAAACTGCAGCTGCAGCTCATGCGGCTGCAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

75

Amino Acids

7.46

Weight (kDa)

5.05

Isoelectric Point (pI)

54.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 3 - 72 1.7e-18 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 210
AluBI AGCT 3 cut(s) 18, 198, 204
AluI AGCT 3 cut(s) 18, 198, 204
Alw26I GTCTC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 48
ApeKI GCWGC 5 cut(s) 48, 195, 198, 201, 213
Asp700I GAANNNNTTC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 134
BbvI GCAGC 5 cut(s) 35, 185, 200, 207, 213
BccI CCATC 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 2 cut(s) 193, 199
BisI GCNGC 7 cut(s) 40, 49, 196, 199, 202, 211, 214
BlsI GCNGC 7 cut(s) 41, 50, 197, 200, 203, 212, 215
BmsI GCATC 1 cut(s) 51
BpiI GAAGAC 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 105
BseGI GGATG 1 cut(s) 66
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 96, 129, 138
BseNI ACTGG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 35, 185, 200, 207, 213
BseYI CCCAGC 1 cut(s) 110
BsmAI GTCTC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 80
BspACI CCGC 2 cut(s) 39, 210
BspCNI CTCAG 3 cut(s) 95, 130, 139
BspMAI CTGCAG 2 cut(s) 197, 203
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 1 cut(s) 122
BstAPI GCANNNNNTGC 2 cut(s) 48, 207
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 82, 138, 147
BstF5I GGATG 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 80
BstSFI CTRYAG 2 cut(s) 193, 199
BstV1I GCAGC 5 cut(s) 35, 185, 200, 207, 213
BstV2I GAAGAC 1 cut(s) 134
BtsCI GGATG 1 cut(s) 66
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 129
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 48
CviAII CATG 1 cut(s) 207
CviJI RGCY 6 cut(s) 18, 48, 186, 198, 204, 213
CviKI_1 RGCY 6 cut(s) 18, 48, 186, 198, 204, 213
DdeI CTNAG 3 cut(s) 82, 138, 147
Eco31I GGTCTC 1 cut(s) 80
FaeI CATG 1 cut(s) 210
FaiI YATR 2 cut(s) 159, 208
FatI CATG 1 cut(s) 206
Fnu4HI GCNGC 7 cut(s) 40, 49, 196, 199, 202, 211, 214
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 40, 49, 196, 199, 202, 211, 214
FspBI CTAG 1 cut(s) 6
GluI GCNGC 7 cut(s) 40, 49, 196, 199, 202, 211, 214
GsaI CCCAGC 1 cut(s) 114
Hin1II CATG 1 cut(s) 210
Hpy188I TCNGA 3 cut(s) 85, 139, 148
HpyCH4IV ACGT 1 cut(s) 131
HpyCH4V TGCA 8 cut(s) 33, 51, 64, 119, 177, 195, 201, 216
HpyF3I CTNAG 3 cut(s) 82, 138, 147
HpySE526I ACGT 1 cut(s) 131
Hsp92II CATG 1 cut(s) 210
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 2 cut(s) 96, 135
Lsp1109I GCAGC 5 cut(s) 35, 185, 200, 207, 213
LweI GCATC 1 cut(s) 51
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 131
MaeIII GTNAC 2 cut(s) 26, 77
MboII GAAGA 1 cut(s) 139
MfeI CAATTG 1 cut(s) 10
MluCI AATT 1 cut(s) 10
MnlI CCTC 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 198
MunI CAATTG 1 cut(s) 10
NlaIII CATG 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 131
PkrI GCNGC 7 cut(s) 41, 50, 197, 200, 203, 212, 215
PspFI CCCAGC 1 cut(s) 110
PstI CTGCAG 2 cut(s) 197, 203
PstNI CAGNNNCTG 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 198
SatI GCNGC 7 cut(s) 40, 49, 196, 199, 202, 211, 214
SetI ASST 4 cut(s) 20, 134, 200, 206
SfaNI GCATC 1 cut(s) 51
SfcI CTRYAG 2 cut(s) 193, 199
SgeI CNNG 8 cut(s) 18, 46, 77, 85, 123, 134, 190, 219
Sse9I AATT 1 cut(s) 10
SsiI CCGC 2 cut(s) 39, 210
SspMI CTAG 1 cut(s) 6
TaiI ACGT 1 cut(s) 134
TasI AATT 1 cut(s) 10
TauI GCSGC 2 cut(s) 42, 213
TscAI CASTG 2 cut(s) 49, 129
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 5 cut(s) 48, 195, 198, 201, 213
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 167
TspRI CASTG 2 cut(s) 49, 129
XmnI GAANNNNTTC 1 cut(s) 131
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.