MD04G1128900.v1.1

metallothionein-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000858
Physical Location & Seq
Forward (+)
21485358 .. 21487393
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1128900.v1.1.491

Sequence Viewer

Length: 243 bp
ATGTCGTCGTGCTGCGGTGGTAAATGTGGTTGCGGGTCCGGCTGCGGCTGCGGCAGTGGCTGCAACGGGTGCGGGATGGCTCCTGATCTGAGCTACATGGAGGGGTCCACCACTGAGACCCTTGTCATGGGAGTTGCTCCCCAGAAGTCGCACTTTGAGGCATCTGAGATGGGAGTTGTAGCTGAGAACGGATGCAAGTGCGGGGATAGCTGCACCTGCAACCCCTGCAAGTGCACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

81

Amino Acids

7.9

Weight (kDa)

5.09

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 3 - 78 1.5e-24 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
Acc36I ACCTGC 1 cut(s) 224
AciI CCGC 6 cut(s) 15, 33, 45, 51, 72, 201
AdeI CACNNNGTG 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 127
AluBI AGCT 3 cut(s) 93, 182, 210
AluI AGCT 3 cut(s) 93, 182, 210
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 1 cut(s) 110
Alw44I GTGCAC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 60
ApaLI GTGCAC 1 cut(s) 232
ApeKI GCWGC 5 cut(s) 12, 42, 48, 60, 210
AspS9I GGNCC 2 cut(s) 36, 105
AvaII GGWCC 2 cut(s) 36, 105
BaeGI GKGCMC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 4 cut(s) 29, 35, 47, 197
BccI CCATC 2 cut(s) 70, 163
BcoDI GTCTC 1 cut(s) 110
BfuAI ACCTGC 1 cut(s) 224
BisI GCNGC 7 cut(s) 13, 43, 46, 49, 52, 61, 211
BlsI GCNGC 7 cut(s) 14, 44, 47, 50, 53, 62, 212
Bme18I GGWCC 2 cut(s) 36, 105
BmgT120I GGNCC 2 cut(s) 36, 105
BmiI GGNNCC 3 cut(s) 37, 81, 106
BmsI GCATC 2 cut(s) 170, 182
BoxI GACNNNNGTC 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 127
BseGI GGATG 2 cut(s) 81, 197
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMII CTCAG 4 cut(s) 80, 105, 156, 174
BseSI GKGCMC 1 cut(s) 236
BseXI GCAGC 4 cut(s) 29, 35, 47, 197
BsgI GTGCAG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 236
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 1 cut(s) 127
BsmAI GTCTC 1 cut(s) 110
Bso31I GGTCTC 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 1 cut(s) 85
BspACI CCGC 6 cut(s) 15, 33, 45, 51, 72, 201
BspCNI CTCAG 4 cut(s) 81, 106, 157, 175
BspLI GGNNCC 3 cut(s) 37, 81, 106
BspMI ACCTGC 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 110
BssMI GATC 1 cut(s) 85
BstAPI GCANNNNNTGC 3 cut(s) 60, 69, 225
BstDEI CTNAG 4 cut(s) 89, 114, 165, 183
BstF5I GGATG 2 cut(s) 81, 197
BstKTI GATC 1 cut(s) 88
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 1 cut(s) 85
BstMWI GCNNNNNNNGC 9 cut(s) 39, 48, 51, 57, 60, 69, 207, 216, 225
BstPAI GACNNNNGTC 1 cut(s) 122
BstSLI GKGCMC 1 cut(s) 236
BstV1I GCAGC 4 cut(s) 29, 35, 47, 197
BtsCI GGATG 2 cut(s) 81, 197
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 2 cut(s) 61, 111
BveI ACCTGC 1 cut(s) 224
CaiI CAGNNNCTG 1 cut(s) 60
Cfr13I GGNCC 2 cut(s) 36, 105
CviAII CATG 2 cut(s) 97, 127
CviJI RGCY 7 cut(s) 42, 48, 60, 80, 93, 182, 210
CviKI_1 RGCY 7 cut(s) 42, 48, 60, 80, 93, 182, 210
DdeI CTNAG 4 cut(s) 89, 114, 165, 183
DpnI GATC 1 cut(s) 87
DpnII GATC 1 cut(s) 85
DraIII CACNNNGTG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 110
Eco47I GGWCC 2 cut(s) 36, 105
FaeI CATG 2 cut(s) 100, 130
FaiI YATR 2 cut(s) 98, 128
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 2 cut(s) 96, 126
FauI CCCGC 3 cut(s) 26, 65, 194
Fnu4HI GCNGC 7 cut(s) 13, 43, 46, 49, 52, 61, 211
FokI GGATG 2 cut(s) 88, 204
Fsp4HI GCNGC 7 cut(s) 13, 43, 46, 49, 52, 61, 211
GluI GCNGC 7 cut(s) 13, 43, 46, 49, 52, 61, 211
HapII CCGG 1 cut(s) 39
Hin1II CATG 2 cut(s) 100, 130
HpaII CCGG 1 cut(s) 39
Hpy166II GTNNAC 2 cut(s) 108, 234
Hpy188I TCNGA 2 cut(s) 90, 166
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 2 cut(s) 108, 234
Hpy99I CGWCG 1 cut(s) 10
HpyCH4V TGCA 6 cut(s) 63, 195, 213, 219, 228, 234
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 48, 51, 57, 60, 69, 207, 216, 225
HpyF3I CTNAG 4 cut(s) 89, 114, 165, 183
Hsp92II CATG 2 cut(s) 100, 130
Kzo9I GATC 1 cut(s) 85
LmnI GCTCC 2 cut(s) 85, 142
LpnPI CCDG 5 cut(s) 52, 96, 155, 229, 238
Lsp1109I GCAGC 4 cut(s) 29, 35, 47, 197
LweI GCATC 2 cut(s) 170, 182
MalI GATC 1 cut(s) 87
MboI GATC 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 236
MnlI CCTC 2 cut(s) 94, 151
MspI CCGG 1 cut(s) 39
MwoI GCNNNNNNNGC 9 cut(s) 39, 48, 51, 57, 60, 69, 207, 216, 225
NdeII GATC 1 cut(s) 85
NlaIII CATG 2 cut(s) 100, 130
NlaIV GGNNCC 3 cut(s) 37, 81, 106
PaqCI CACCTGC 1 cut(s) 224
PkrI GCNGC 7 cut(s) 14, 44, 47, 50, 53, 62, 212
PshAI GACNNNNGTC 1 cut(s) 122
PspN4I GGNNCC 3 cut(s) 37, 81, 106
PspPI GGNCC 2 cut(s) 36, 105
PstNI CAGNNNCTG 1 cut(s) 60
SatI GCNGC 7 cut(s) 13, 43, 46, 49, 52, 61, 211
Sau3AI GATC 1 cut(s) 85
Sau96I GGNCC 2 cut(s) 36, 105
SduI GDGCHC 1 cut(s) 236
SetI ASST 4 cut(s) 95, 184, 212, 218
SfaNI GCATC 2 cut(s) 170, 182
SinI GGWCC 2 cut(s) 36, 105
SsiI CCGC 6 cut(s) 15, 33, 45, 51, 72, 201
TauI GCSGC 2 cut(s) 48, 54
TscAI CASTG 2 cut(s) 61, 118
TseI GCWGC 5 cut(s) 12, 42, 48, 60, 210
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 2 cut(s) 61, 118
VneI GTGCAC 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 36, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.