MD03G1094500.v1.1

Metallothionein-like protein type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000858
Physical Location & Seq
Reverse (-)
7944811 .. 7945713
903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1094500.v1.1.491

Sequence Viewer

Length: 228 bp
ATGTCTTGCAATCGTAGTTGTGGAAGTGACTGCAAGTGCGGCAGTGGCTGCAAGTGTGGCATATACCCTGACTTGAGCTACTCAGAGACCACTTCCACTGAAACTGTCATTGCCGGAGTTGCCCTTGTGAAAATGTTCTCTGAGGGGTCTGAGATGAGCTATGGAGCAGAGAATGGCTGCAAGTGTGGCTCAAGCTGCAGCTGCAGCTCATGTGGCTGCCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

76

Amino Acids

7.67

Weight (kDa)

5.64

Isoelectric Point (pI)

71.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 4 - 73 8.6e-18 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 39
AluBI AGCT 5 cut(s) 78, 159, 195, 201, 207
AluI AGCT 5 cut(s) 78, 159, 195, 201, 207
Alw26I GTCTC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 48
ApeKI GCWGC 7 cut(s) 48, 177, 195, 198, 201, 204, 216
Asp700I GAANNNNTTC 1 cut(s) 134
BbvI GCAGC 7 cut(s) 35, 164, 182, 188, 203, 210, 216
BcoDI GTCTC 1 cut(s) 80
BfmI CTRYAG 2 cut(s) 196, 202
BisI GCNGC 8 cut(s) 40, 49, 178, 196, 199, 202, 205, 217
BlsI GCNGC 8 cut(s) 41, 50, 179, 197, 200, 203, 206, 218
BpuEI CTTGAG 2 cut(s) 94, 175
BsaI GGTCTC 1 cut(s) 80
Bse3DI GCAATG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 3 cut(s) 96, 132, 141
BseXI GCAGC 7 cut(s) 35, 164, 182, 188, 203, 210, 216
BsiSI CCGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 80
BspACI CCGC 1 cut(s) 39
BspCNI CTCAG 3 cut(s) 95, 133, 142
BspMAI CTGCAG 2 cut(s) 200, 206
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 106
BstAPI GCANNNNNTGC 1 cut(s) 48
BstDEI CTNAG 3 cut(s) 82, 141, 150
BstMAI GTCTC 1 cut(s) 80
BstSFI CTRYAG 2 cut(s) 196, 202
BstV1I GCAGC 7 cut(s) 35, 164, 182, 188, 203, 210, 216
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 96
CaiI CAGNNNCTG 1 cut(s) 48
CviAII CATG 1 cut(s) 210
CviJI RGCY 9 cut(s) 48, 78, 159, 177, 189, 195, 201, 207, 216
CviKI_1 RGCY 9 cut(s) 48, 78, 159, 177, 189, 195, 201, 207, 216
DdeI CTNAG 3 cut(s) 82, 141, 150
Eco31I GGTCTC 1 cut(s) 80
FaeI CATG 1 cut(s) 213
FaiI YATR 4 cut(s) 62, 64, 162, 211
FatI CATG 1 cut(s) 209
Fnu4HI GCNGC 8 cut(s) 40, 49, 178, 196, 199, 202, 205, 217
Fsp4HI GCNGC 8 cut(s) 40, 49, 178, 196, 199, 202, 205, 217
GluI GCNGC 8 cut(s) 40, 49, 178, 196, 199, 202, 205, 217
HapII CCGG 1 cut(s) 114
Hin1II CATG 1 cut(s) 213
HpaII CCGG 1 cut(s) 114
Hpy188I TCNGA 3 cut(s) 85, 142, 151
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 6 cut(s) 9, 33, 51, 180, 198, 204
HpyF3I CTNAG 3 cut(s) 82, 141, 150
Hsp92II CATG 1 cut(s) 213
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 2 cut(s) 81, 127
Lsp1109I GCAGC 7 cut(s) 35, 164, 182, 188, 203, 210, 216
MaeIII GTNAC 1 cut(s) 26
MnlI CCTC 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 114
NlaIII CATG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 26
PdmI GAANNNNTTC 1 cut(s) 134
PkrI GCNGC 8 cut(s) 41, 50, 179, 197, 200, 203, 206, 218
PstI CTGCAG 2 cut(s) 200, 206
PstNI CAGNNNCTG 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 201
SatI GCNGC 8 cut(s) 40, 49, 178, 196, 199, 202, 205, 217
SetI ASST 5 cut(s) 80, 161, 197, 203, 209
SfcI CTRYAG 2 cut(s) 196, 202
SmlI CTYRAG 2 cut(s) 73, 190
SmoI CTYRAG 2 cut(s) 73, 190
SsiI CCGC 1 cut(s) 39
TaaI ACNGT 1 cut(s) 106
TauI GCSGC 1 cut(s) 42
TscAI CASTG 2 cut(s) 49, 103
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 7 cut(s) 48, 177, 195, 198, 201, 204, 216
Tsp45I GTSAC 1 cut(s) 26
TspRI CASTG 2 cut(s) 49, 103
XmnI GAANNNNTTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.