Rw5G039800

Metallothionein-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000858
Physical Location & Seq
Forward (+)
70347214 .. 70348227
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039800.1

Sequence Viewer

Length: 231 bp
ATGTCTGGGTGTGGATCAACAAGCTGCAAATGCGGTTCTAGCTGCAAATGCGGCTCTAGCTGCAACTGTGGAAACTACCCAGATCTTGAGAGCAGCACAACAGCAACCATCATTGCTGGGGTTCCATCAACCAACATGTACTTTGAGGAGTCAGAGATGAGCTTCGGTGCTGAAAATGGCTGCAAATGCGGCCAAAGCTGCAGCTGCACCTCATGCGGCTGCCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

76

Amino Acids

7.7

Weight (kDa)

5.05

Isoelectric Point (pI)

86.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 1 - 74 1.9e-15 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 51, 189, 216
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 190
AfaI GTAC 1 cut(s) 140
AflIII ACRYGT 1 cut(s) 135
AluBI AGCT 6 cut(s) 24, 42, 60, 162, 198, 204
AluI AGCT 6 cut(s) 24, 42, 60, 162, 198, 204
AlwI GGATC 1 cut(s) 22
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 9 cut(s) 24, 42, 60, 93, 180, 198, 201, 204, 219
BbvI GCAGC 9 cut(s) 11, 29, 47, 105, 167, 185, 191, 206, 213
BccI CCATC 2 cut(s) 116, 133
BfaI CTAG 2 cut(s) 39, 57
BfmI CTRYAG 1 cut(s) 199
BglII AGATCT 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 9 cut(s) 11, 29, 47, 105, 167, 185, 191, 206, 213
BseYI CCCAGC 1 cut(s) 116
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 1 cut(s) 192
BsnI GGCC 1 cut(s) 192
Bsp143I GATC 2 cut(s) 14, 82
BspACI CCGC 4 cut(s) 33, 51, 189, 216
BspANI GGCC 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 123
BspMAI CTGCAG 1 cut(s) 203
BspPI GGATC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 111
BssMI GATC 2 cut(s) 14, 82
Bst4CI ACNGT 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 213
BstKTI GATC 2 cut(s) 17, 85
BstMBI GATC 2 cut(s) 14, 82
BstNSI RCATGY 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 199
BstV1I GCAGC 9 cut(s) 11, 29, 47, 105, 167, 185, 191, 206, 213
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuRI GGCC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 136, 213
CviQI GTAC 1 cut(s) 139
DpnI GATC 2 cut(s) 16, 84
DpnII GATC 2 cut(s) 14, 82
EaeI YGGCCR 1 cut(s) 190
FaeI CATG 2 cut(s) 139, 216
FaiI YATR 2 cut(s) 137, 214
FatI CATG 2 cut(s) 135, 212
FspBI CTAG 2 cut(s) 39, 57
GsaI CCCAGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 192
Hin1II CATG 2 cut(s) 139, 216
HinfI GANTC 1 cut(s) 149
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 6 cut(s) 27, 45, 63, 183, 201, 207
Hsp92II CATG 2 cut(s) 139, 216
Kzo9I GATC 2 cut(s) 14, 82
LpnPI CCDG 2 cut(s) 93, 102
Lsp1109I GCAGC 9 cut(s) 11, 29, 47, 105, 167, 185, 191, 206, 213
MaeI CTAG 2 cut(s) 39, 57
MalI GATC 2 cut(s) 16, 84
MboI GATC 2 cut(s) 14, 82
MflI RGATCY 1 cut(s) 82
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 2 cut(s) 139, 220
MspA1I CMGCKG 1 cut(s) 204
NdeII GATC 2 cut(s) 14, 82
NlaIII CATG 2 cut(s) 139, 216
NlaIV GGNNCC 1 cut(s) 123
NspI RCATGY 1 cut(s) 139
PciI ACATGT 1 cut(s) 135
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PscI ACATGT 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 123
PstI CTGCAG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 204
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
Sau3AI GATC 2 cut(s) 14, 82
SchI GAGTC 1 cut(s) 158
SetI ASST 7 cut(s) 26, 44, 62, 164, 200, 206, 212
SfcI CTRYAG 1 cut(s) 199
SgeI CNNG 9 cut(s) 18, 33, 51, 69, 92, 98, 129, 148, 225
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SsiI CCGC 4 cut(s) 33, 51, 189, 216
SspMI CTAG 2 cut(s) 39, 57
TaaI ACNGT 1 cut(s) 68
TatI WGTACW 1 cut(s) 138
TauI GCSGC 3 cut(s) 54, 192, 219
TseI GCWGC 9 cut(s) 24, 42, 60, 93, 180, 198, 201, 204, 219
XceI RCATGY 1 cut(s) 139
XspI CTAG 2 cut(s) 39, 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.