MD11G1109200.v1.1

Metallothionein-like protein type 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000858
Physical Location & Seq
Reverse (-)
9653383 .. 9654339
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1109200.v1.1.491

Sequence Viewer

Length: 228 bp
ATGTCTTGCAATTGTAGCTGTGGTAGCGACTGCAAGTGCGGCAGTGGCTGCAAATGTGGCATGTACCCTGACTTGAGTTACTCAGAGACCACTTCCACTGAGACTATAATTGATGGGGTTGCACCAATGAAGATGTTCTACGAGGGGTCTGAGATGAACTATGGAGCAGAGAACGACTGCAAGTGTGGCTCAAACTGCAGCTGCAGCTCATGCGGTTGCCACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallothio_2

Protein Analysis

76

Amino Acids

7.89

Weight (kDa)

4.6

Isoelectric Point (pI)

60.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallothio_2 PF01439 3 - 73 1.5e-20 Metallothionein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 213
AfaI GTAC 1 cut(s) 65
AluBI AGCT 3 cut(s) 18, 201, 207
AluI AGCT 3 cut(s) 18, 201, 207
Alw26I GTCTC 2 cut(s) 80, 95
AlwNI CAGNNNCTG 1 cut(s) 48
ApeKI GCWGC 4 cut(s) 48, 198, 201, 204
Asp700I GAANNNNTTC 1 cut(s) 134
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
BbvI GCAGC 4 cut(s) 35, 188, 210, 216
BccI CCATC 1 cut(s) 107
BcoDI GTCTC 2 cut(s) 80, 95
BfmI CTRYAG 2 cut(s) 196, 202
BisI GCNGC 5 cut(s) 40, 49, 199, 202, 205
BlsI GCNGC 5 cut(s) 41, 50, 200, 203, 206
BpuEI CTTGAG 1 cut(s) 94
BsaI GGTCTC 1 cut(s) 80
BseMII CTCAG 3 cut(s) 90, 96, 141
BseXI GCAGC 4 cut(s) 35, 188, 210, 216
BsmAI GTCTC 2 cut(s) 80, 95
Bso31I GGTCTC 1 cut(s) 80
BspACI CCGC 2 cut(s) 39, 213
BspCNI CTCAG 3 cut(s) 91, 95, 142
BspMAI CTGCAG 2 cut(s) 200, 206
BspTNI GGTCTC 1 cut(s) 80
BstAPI GCANNNNNTGC 2 cut(s) 48, 210
BstDEI CTNAG 3 cut(s) 82, 99, 150
BstMAI GTCTC 2 cut(s) 80, 95
BstNSI RCATGY 1 cut(s) 64
BstSFI CTRYAG 2 cut(s) 196, 202
BstV1I GCAGC 4 cut(s) 35, 188, 210, 216
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 96
CaiI CAGNNNCTG 1 cut(s) 48
Csp6I GTAC 1 cut(s) 64
CviAII CATG 2 cut(s) 61, 210
CviJI RGCY 5 cut(s) 18, 48, 189, 201, 207
CviKI_1 RGCY 5 cut(s) 18, 48, 189, 201, 207
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 3 cut(s) 82, 99, 150
Eco31I GGTCTC 1 cut(s) 80
FaeI CATG 2 cut(s) 64, 213
FaiI YATR 4 cut(s) 62, 107, 162, 211
FatI CATG 2 cut(s) 60, 209
Fnu4HI GCNGC 5 cut(s) 40, 49, 199, 202, 205
Fsp4HI GCNGC 5 cut(s) 40, 49, 199, 202, 205
GluI GCNGC 5 cut(s) 40, 49, 199, 202, 205
Hin1II CATG 2 cut(s) 64, 213
Hpy188I TCNGA 2 cut(s) 85, 151
HpyCH4V TGCA 7 cut(s) 9, 33, 51, 122, 180, 198, 204
HpyF3I CTNAG 3 cut(s) 82, 99, 150
Hsp92II CATG 2 cut(s) 64, 213
LmnI GCTCC 1 cut(s) 164
LpnPI CCDG 1 cut(s) 81
Lsp1109I GCAGC 4 cut(s) 35, 188, 210, 216
MaeIII GTNAC 1 cut(s) 77
MboII GAAGA 1 cut(s) 142
MfeI CAATTG 1 cut(s) 10
MluCI AATT 2 cut(s) 10, 108
MnlI CCTC 1 cut(s) 136
MroXI GAANNNNTTC 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 201
MunI CAATTG 1 cut(s) 10
NlaIII CATG 2 cut(s) 64, 213
NspI RCATGY 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 134
PkrI GCNGC 5 cut(s) 41, 50, 200, 203, 206
PstI CTGCAG 2 cut(s) 200, 206
PstNI CAGNNNCTG 1 cut(s) 48
PvuII CAGCTG 1 cut(s) 201
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SatI GCNGC 5 cut(s) 40, 49, 199, 202, 205
SetI ASST 3 cut(s) 20, 203, 209
SfcI CTRYAG 2 cut(s) 196, 202
SgeI CNNG 8 cut(s) 18, 46, 73, 80, 85, 154, 193, 222
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 2 cut(s) 10, 108
SsiI CCGC 2 cut(s) 39, 213
TasI AATT 2 cut(s) 10, 108
TauI GCSGC 1 cut(s) 42
TscAI CASTG 2 cut(s) 49, 103
TseI GCWGC 4 cut(s) 48, 198, 201, 204
TspDTI ATGAA 2 cut(s) 143, 170
TspRI CASTG 2 cut(s) 49, 103
XceI RCATGY 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.