MD12G1194500.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0022398
Physical Location & Seq
Reverse (-)
27587576 .. 27588121
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1194500.v1.1.491

Sequence Viewer

Length: 546 bp
ATGCTGAAACAAAAGCCGTTACTCGCCGCTGCCCTGGCAGTGCTCGGAGTTCTTCTGGCCATATTTTTTATAATGCTGATCCTAGGGCTTACGAAGTACAAGCCAAAGGACCCCATAACGCGTGTGGATGCAGTGGTCTTAAGAAACCTAGACATTTCTCACCAGGCGCCTAAACTCATCACTGTGGATATGTCTCTCTCCATCAAGAACCAGAACAAGGTGGTCTTCAAGCCCACCAAGGGCACTGCAGTCTTGTTCTACAAGGGCGTGAACGTCGGTGAGGCAGCCATAGAGATAGGAAAGATGGCGCCGGGAGCCACCATCAACACGAATGTTACATTCACCCCTTTGGCTGATCATTTTCAAAGAAACCCCGAGGTTAGTTTTGATCTGTTGGCGGGTAGGATGCCCTTCAACACTTTCACCAAAGTCCCCGGAAAAGTGAAGGTTTTTGGAATGGCTAAGGTTGGTATGATCTCCACCAGGTTGTGCGGTTTTGAAATCGATATCCGTAGCAAGACTATTGGGGACCATAGGTGCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

182

Amino Acids

19.74

Weight (kDa)

9.97

Isoelectric Point (pI)

24.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022398)

Species Orthologous Gene IDs
malus_domestica MD04G1179400.v1.1 MD12G1194500.v1.1
prunus_persica Prupe.6G299300_v2.0.a1 Prupe.6G299400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
Acc16I TGCGCA 1 cut(s) 541
AccB1I GGYRCC 2 cut(s) 166, 307
AccII CGCG 1 cut(s) 121
AciI CCGC 3 cut(s) 27, 398, 492
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 57
AcyI GRCGYC 2 cut(s) 167, 308
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 217, 239
AflII CTTAAG 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 119
AgsI TTSAA 4 cut(s) 229, 365, 415, 500
AjnI CCWGG 3 cut(s) 33, 162, 482
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 2 cut(s) 29, 284
AspA2I CCTAGG 1 cut(s) 82
AspLEI GCGC 3 cut(s) 169, 310, 542
AspS9I GGNCC 2 cut(s) 109, 529
AsuC2I CCSGG 2 cut(s) 312, 435
AsuHPI GGTGA 4 cut(s) 152, 290, 334, 415
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 109, 529
AvrII CCTAGG 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 2 cut(s) 166, 307
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 2 cut(s) 16, 296
BccI CCATC 3 cut(s) 209, 298, 329
BciT130I CCWGG 3 cut(s) 35, 164, 484
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 2 cut(s) 312, 435
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 2 cut(s) 83, 149
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 2 cut(s) 170, 311
BfrI CTTAAG 1 cut(s) 139
BisI GCNGC 3 cut(s) 27, 30, 285
BlnI CCTAGG 1 cut(s) 82
BlsI GCNGC 3 cut(s) 28, 31, 286
Bme1390I CCNGG 5 cut(s) 35, 164, 312, 435, 484
Bme18I GGWCC 2 cut(s) 109, 529
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 109, 529
BmiI GGNNCC 5 cut(s) 111, 168, 309, 316, 530
BmrFI CCNGG 5 cut(s) 35, 164, 312, 435, 484
BmsI GCATC 2 cut(s) 118, 396
BpiI GAAGAC 1 cut(s) 217
Bpu10I CCTNAGC 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 312, 435
Bsa29I ATCGAT 1 cut(s) 504
BsaHI GRCGYC 2 cut(s) 167, 308
BsaJI CCNNGG 5 cut(s) 33, 82, 237, 375, 433
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 239
BseBI CCWGG 3 cut(s) 35, 164, 484
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 5 cut(s) 33, 82, 237, 375, 433
BseGI GGATG 2 cut(s) 133, 411
BseLI CCNNNNNNNGG 2 cut(s) 217, 239
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 2 cut(s) 16, 296
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 1 cut(s) 59
BshNI GGYRCC 2 cut(s) 166, 307
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 2 cut(s) 311, 435
BslFI GGGAC 2 cut(s) 416, 542
BslI CCNNNNNNNGG 2 cut(s) 217, 239
BsmAI GTCTC 1 cut(s) 198
BsmFI GGGAC 2 cut(s) 416, 542
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 45, 245
Bsp143I GATC 4 cut(s) 78, 355, 388, 474
BspACI CCGC 3 cut(s) 27, 398, 492
BspANI GGCC 1 cut(s) 59
BspDI ATCGAT 1 cut(s) 504
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 111, 168, 309, 316, 530
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 1 cut(s) 73
BspT107I GGYRCC 2 cut(s) 166, 307
BspTI CTTAAG 1 cut(s) 139
BssECI CCNNGG 5 cut(s) 33, 82, 237, 375, 433
BssMI GATC 4 cut(s) 78, 355, 388, 474
BssNI GRCGYC 2 cut(s) 167, 308
BssT1I CCWWGG 2 cut(s) 82, 237
Bst2UI CCWGG 3 cut(s) 35, 164, 484
Bst4CI ACNGT 1 cut(s) 184
BstACI GRCGYC 2 cut(s) 167, 308
BstAFI CTTAAG 1 cut(s) 139
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 133, 411
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 2 cut(s) 170, 311
BstHHI GCGC 3 cut(s) 169, 310, 542
BstKTI GATC 4 cut(s) 81, 358, 391, 477
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 4 cut(s) 78, 355, 388, 474
BstMWI GCNNNNNNNGC 2 cut(s) 35, 314
BstNI CCWGG 3 cut(s) 35, 164, 484
BstSCI CCNGG 5 cut(s) 33, 162, 310, 433, 482
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 2 cut(s) 16, 296
BstV2I GAAGAC 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 504
BsuRI GGCC 1 cut(s) 59
BsuTUI ATCGAT 1 cut(s) 504
BtsCI GGATG 2 cut(s) 133, 411
BtsI GCAGTG 3 cut(s) 45, 138, 243
BtsIMutI CAGTG 4 cut(s) 45, 138, 180, 243
CfoI GCGC 3 cut(s) 169, 310, 542
Cfr13I GGNCC 2 cut(s) 109, 529
ClaI ATCGAT 1 cut(s) 504
CsiI ACCWGGT 1 cut(s) 482
Csp6I GTAC 1 cut(s) 97
CviJI RGCY 9 cut(s) 16, 59, 88, 103, 232, 287, 317, 353, 461
CviKI_1 RGCY 9 cut(s) 16, 59, 88, 103, 232, 287, 317, 353, 461
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 462
DinI GGCGCC 2 cut(s) 168, 309
DpnI GATC 4 cut(s) 80, 357, 390, 476
DpnII GATC 4 cut(s) 78, 355, 388, 474
EaeI YGGCCR 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 82, 237
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 109, 529
Eco88I CYCGRG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 3 cut(s) 33, 162, 482
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 2 cut(s) 82, 237
EgeI GGCGCC 2 cut(s) 168, 309
EheI GGCGCC 2 cut(s) 168, 309
ErhI CCWWGG 2 cut(s) 82, 237
FaiI YATR 8 cut(s) 62, 71, 116, 191, 290, 473, 534, 544
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 2 cut(s) 416, 542
FauI CCCGC 1 cut(s) 391
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 3 cut(s) 27, 30, 285
FokI GGATG 2 cut(s) 140, 418
Fsp4HI GCNGC 3 cut(s) 27, 30, 285
FspAI RTGCGCAY 1 cut(s) 541
FspBI CTAG 2 cut(s) 83, 149
FspI TGCGCA 1 cut(s) 541
GlaI GCGC 3 cut(s) 168, 309, 541
GluI GCNGC 3 cut(s) 27, 30, 285
HaeII RGCGCY 2 cut(s) 170, 311
HaeIII GGCC 1 cut(s) 59
HapII CCGG 2 cut(s) 311, 435
HhaI GCGC 3 cut(s) 169, 310, 542
Hin1I GRCGYC 2 cut(s) 167, 308
Hin6I GCGC 3 cut(s) 167, 308, 540
HinP1I GCGC 3 cut(s) 167, 308, 540
HpaII CCGG 2 cut(s) 311, 435
HphI GGTGA 4 cut(s) 152, 290, 334, 415
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 47
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 421, 439
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 131, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 314
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 273
Hsp92I GRCGYC 2 cut(s) 167, 308
HspAI GCGC 3 cut(s) 167, 308, 540
KasI GGCGCC 2 cut(s) 166, 307
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 4 cut(s) 78, 355, 388, 474
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 2 cut(s) 16, 296
LweI GCATC 2 cut(s) 118, 396
MabI ACCWGGT 1 cut(s) 482
MaeI CTAG 2 cut(s) 83, 149
MaeII ACGT 1 cut(s) 273
MaeIII GTNAC 2 cut(s) 18, 334
MalI GATC 4 cut(s) 80, 357, 390, 476
MboI GATC 4 cut(s) 78, 355, 388, 474
MboII GAAGA 2 cut(s) 44, 217
MhlI GDGCHC 2 cut(s) 45, 245
MlsI TGGCCA 1 cut(s) 59
MluI ACGCGT 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 59
Mly113I GGCGCC 2 cut(s) 167, 308
MnlI CCTC 2 cut(s) 274, 370
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 1 cut(s) 140
MslI CAYNNNNRTG 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 139
MspI CCGG 2 cut(s) 311, 435
MspR9I CCNGG 5 cut(s) 35, 164, 312, 435, 484
MvaI CCWGG 3 cut(s) 35, 164, 484
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 35, 314
NarI GGCGCC 2 cut(s) 167, 308
NciI CCSGG 2 cut(s) 312, 435
NdeII GATC 4 cut(s) 78, 355, 388, 474
NlaIV GGNNCC 5 cut(s) 111, 168, 309, 316, 530
NsbI TGCGCA 1 cut(s) 541
PkrI GCNGC 3 cut(s) 28, 31, 286
PluTI GGCGCC 2 cut(s) 170, 311
PpuMI RGGWCCY 1 cut(s) 109
PsiI TTATAA 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 3 cut(s) 33, 162, 482
PspGI CCWGG 3 cut(s) 33, 162, 482
PspN4I GGNNCC 5 cut(s) 111, 168, 309, 316, 530
PspPI GGNCC 2 cut(s) 109, 529
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 250
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 3 cut(s) 27, 30, 285
Sau3AI GATC 4 cut(s) 78, 355, 388, 474
Sau96I GGNCC 2 cut(s) 109, 529
ScrFI CCNGG 5 cut(s) 35, 164, 312, 435, 484
SduI GDGCHC 2 cut(s) 45, 245
SetI ASST 8 cut(s) 150, 222, 276, 381, 450, 468, 488, 539
SexAI ACCWGGT 1 cut(s) 482
SfaNI GCATC 2 cut(s) 118, 396
SfcI CTRYAG 1 cut(s) 246
SfoI GGCGCC 2 cut(s) 168, 309
SinI GGWCC 2 cut(s) 109, 529
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SsiI CCGC 3 cut(s) 27, 398, 492
SspDI GGCGCC 2 cut(s) 166, 307
SspMI CTAG 2 cut(s) 83, 149
StyD4I CCNGG 5 cut(s) 33, 162, 310, 433, 482
StyI CCWWGG 2 cut(s) 82, 237
TaaI ACNGT 1 cut(s) 184
TaiI ACGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 504
TatI WGTACW 1 cut(s) 96
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TscAI CASTG 4 cut(s) 45, 138, 187, 250
TseI GCWGC 2 cut(s) 29, 284
TspGWI ACGGA 1 cut(s) 500
TspRI CASTG 4 cut(s) 45, 138, 187, 250
Vha464I CTTAAG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 109, 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XmaJI CCTAGG 1 cut(s) 82
XspI CTAG 2 cut(s) 83, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.