Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0022408

Physical Location & Seq

Genomic Coordinates Forward (+)

28096170 .. 28097185

Gene Structure 1016 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1200000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 696 bp

ATGGCGAGCGGCAAGTCAGTGGTGGCTCTGATCAAGGCCCTGAAGCCGGCATTCCGCTCCGGCAACGACAACGACAAAGTCGCCCTCGCCGTCCACGCCTACTTCTTGGCCTCCGGCTACGACCTCACCGCCACTGGTCCCCACGCCTTTTCCCCTGCCGCCCTCAACTCTTCTTCCACAGATGAAGTGGGTATTGATGGATGGAACGAACTCGAAGACCAGTACGCATTCGTGTACGTGAACCTGGAAGAGGGTTCGAAGAAGGTCCGGGTCAAGTGCCTTGTGATGGAGCATAAACTGCATGTGTTTTGCGCTGTGGCTGATGGGTCTTCTAAGCATGTCGAACTAGAGTTGAGTTTTTGTGCAACTGGCGTTGGGAATTATGTAGAGGAAACCGGGGGCGGAAATTACTCTAGACAGTTCAAGAATATGGAGTTGCTGGTGAAAATTCTCGACTCTTTGCACCCCTTGAGTTCAGAAACAAGTGAGAGTCGCTGTGAGACGAGCATCAGAACTGAGCCTGCTCCTCAGACACATCTTTCAGGGTCTGATCTCTTTCCCTCTCTCTGTTTCATTTACGATCGATTAACAAATGCAACCAATCATGTGAAAAAAAATTGTATAAACTGCAGTTTGTATGCAATACTAGTTCAATTTCAGTCGCCTGAGATGTTTATTAGTTTTCTGTTTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009733 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010605 GO:0012505 GO:0016020 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032870 GO:0042176 GO:0042177 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051716 GO:0060255 GO:0061136 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901799 GO:1903050 GO:1903051 GO:1903362 GO:1903363

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

PI31_Prot_C PI31_Prot_N

Protein Analysis

232

Amino Acids

25.32

Weight (kDa)

5.59

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI31_Prot_N	PF11566	23 - 119	2.8e-13	PI31 proteasome regulator N-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0022408)

Species	Orthologous Gene IDs
malus_domestica	MD12G1200000.v1.1 MD17G1162600.v1.1
prunus_persica	Prupe.6G304800_v2.0.a1
pyrus_communis	pycom12g18670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	9, 57
AciI	CCGC	5 cut(s)	9, 55, 129, 159, 402
AcsI	RAATTY	1 cut(s)	447
AcuI	CTGAAG	1 cut(s)	62
AfaI	GTAC	2 cut(s)	224, 236
AfiI	CCNNNNNNNGG	3 cut(s)	46, 250, 286
AgsI	TTSAA	2 cut(s)	424, 653
AhlI	ACTAGT	1 cut(s)	646
AjnI	CCWGG	1 cut(s)	243
Alw26I	GTCTC	1 cut(s)	494
AlwNI	CAGNNNCTG	1 cut(s)	548
AoxI	GGCC	2 cut(s)	36, 108
ApoI	RAATTY	1 cut(s)	447
AspLEI	GCGC	1 cut(s)	314
AspS9I	GGNCC	3 cut(s)	37, 137, 265
AsuC2I	CCSGG	2 cut(s)	269, 397
AsuHPI	GGTGA	2 cut(s)	118, 454
AsuII	TTCGAA	1 cut(s)	257
AvaII	GGWCC	2 cut(s)	137, 265
BbsI	GAAGAC	2 cut(s)	222, 321
BccI	CCATC	4 cut(s)	191, 195, 280, 317
BceAI	ACGGC	1 cut(s)	74
BciT130I	CCWGG	1 cut(s)	245
BclI	TGATCA	1 cut(s)	30
BcnI	CCSGG	2 cut(s)	269, 397
BcoDI	GTCTC	1 cut(s)	494
BcuI	ACTAGT	1 cut(s)	646
BfaI	CTAG	3 cut(s)	347, 414, 647
BfmI	CTRYAG	1 cut(s)	628
BisI	GCNGC	2 cut(s)	10, 159
BlsI	GCNGC	2 cut(s)	11, 160
Bme1390I	CCNGG	3 cut(s)	245, 269, 397
Bme18I	GGWCC	2 cut(s)	137, 265
BmgT120I	GGNCC	3 cut(s)	37, 137, 265
BmiI	GGNNCC	1 cut(s)	139
BmrFI	CCNGG	3 cut(s)	245, 269, 397
BmsI	GCATC	1 cut(s)	516
BpiI	GAAGAC	2 cut(s)	222, 321
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	1 cut(s)	490
BpuMI	CCSGG	2 cut(s)	269, 397
Bsa29I	ATCGAT	1 cut(s)	583
BsaAI	YACGTR	1 cut(s)	238
BsaJI	CCNNGG	1 cut(s)	396
Bsc4I	CCNNNNNNNGG	3 cut(s)	46, 250, 286
Bse118I	RCCGGY	1 cut(s)	46
Bse1I	ACTGG	3 cut(s)	139, 220, 373
BseBI	CCWGG	1 cut(s)	245
BseCI	ATCGAT	1 cut(s)	583
BseDI	CCNNGG	1 cut(s)	396
BseGI	GGATG	1 cut(s)	206
BseLI	CCNNNNNNNGG	3 cut(s)	46, 250, 286
BseMII	CTCAG	3 cut(s)	507, 542, 657
BseNI	ACTGG	3 cut(s)	139, 220, 373
BseRI	GAGGAG	1 cut(s)	516
Bsh1285I	CGRYCG	1 cut(s)	583
BshFI	GGCC	2 cut(s)	38, 110
BshVI	ATCGAT	1 cut(s)	583
BsiEI	CGRYCG	1 cut(s)	583
BsiSI	CCGG	5 cut(s)	47, 60, 114, 268, 396
BslFI	GGGAC	1 cut(s)	123
BslI	CCNNNNNNNGG	3 cut(s)	46, 250, 286
BsmAI	GTCTC	1 cut(s)	494
BsmBI	CGTCTC	1 cut(s)	494
BsmFI	GGGAC	1 cut(s)	123
BsmI	GAATGC	2 cut(s)	50, 227
BsnI	GGCC	2 cut(s)	38, 110
Bsp119I	TTCGAA	1 cut(s)	257
Bsp143I	GATC	3 cut(s)	30, 550, 580
BspACI	CCGC	5 cut(s)	9, 55, 129, 159, 402
BspANI	GGCC	2 cut(s)	38, 110
BspCNI	CTCAG	3 cut(s)	508, 541, 658
BspDI	ATCGAT	1 cut(s)	583
BspLI	GGNNCC	1 cut(s)	139
BspMAI	CTGCAG	1 cut(s)	632
BspT104I	TTCGAA	1 cut(s)	257
BsrBI	CCGCTC	2 cut(s)	9, 57
BsrFI	RCCGGY	1 cut(s)	46
BsrI	ACTGG	3 cut(s)	139, 220, 373
BssAI	RCCGGY	1 cut(s)	46
BssECI	CCNNGG	1 cut(s)	396
BssMI	GATC	3 cut(s)	30, 550, 580
Bst2UI	CCWGG	1 cut(s)	245
Bst4CI	ACNGT	1 cut(s)	420
Bst6I	CTCTTC	2 cut(s)	175, 243
BstAPI	GCANNNNNTGC	1 cut(s)	298
BstBAI	YACGTR	1 cut(s)	238
BstBI	TTCGAA	1 cut(s)	257
BstC8I	GCNNGC	3 cut(s)	7, 48, 522
BstDEI	CTNAG	4 cut(s)	333, 516, 528, 666
BstENI	CCTNNNNNAGG	1 cut(s)	248
BstF5I	GGATG	1 cut(s)	206
BstHHI	GCGC	1 cut(s)	314
BstKTI	GATC	3 cut(s)	33, 553, 583
BstMAI	GTCTC	1 cut(s)	494
BstMBI	GATC	3 cut(s)	30, 550, 580
BstMCI	CGRYCG	1 cut(s)	583
BstMWI	GCNNNNNNNGC	2 cut(s)	95, 298
BstNI	CCWGG	1 cut(s)	245
BstNSI	RCATGY	2 cut(s)	305, 341
BstSCI	CCNGG	3 cut(s)	243, 267, 395
BstSFI	CTRYAG	1 cut(s)	628
BstV2I	GAAGAC	2 cut(s)	222, 321
Bsu15I	ATCGAT	1 cut(s)	583
BsuRI	GGCC	2 cut(s)	38, 110
BsuTUI	ATCGAT	1 cut(s)	583
BtsCI	GGATG	1 cut(s)	206
BtsIMutI	CAGTG	2 cut(s)	24, 132
Cac8I	GCNNGC	3 cut(s)	7, 48, 522
CaiI	CAGNNNCTG	1 cut(s)	548
CfoI	GCGC	1 cut(s)	314
Cfr10I	RCCGGY	1 cut(s)	46
Cfr13I	GGNCC	3 cut(s)	37, 137, 265
ClaI	ATCGAT	1 cut(s)	583
Csp6I	GTAC	2 cut(s)	223, 235
CviAII	CATG	3 cut(s)	302, 338, 605
CviJI	RGCY	7 cut(s)	26, 38, 46, 110, 117, 320, 520
CviKI_1	RGCY	7 cut(s)	26, 38, 46, 110, 117, 320, 520
CviQI	GTAC	2 cut(s)	223, 235
DdeI	CTNAG	4 cut(s)	333, 516, 528, 666
DpnI	GATC	3 cut(s)	32, 552, 582
DpnII	GATC	3 cut(s)	30, 550, 580
Eam1104I	CTCTTC	2 cut(s)	175, 243
EarI	CTCTTC	2 cut(s)	175, 243
EciI	GGCGGA	1 cut(s)	417
Eco47I	GGWCC	2 cut(s)	137, 265
Eco57I	CTGAAG	1 cut(s)	62
EcoNI	CCTNNNNNAGG	1 cut(s)	248
EcoO109I	RGGNCCY	1 cut(s)	37
EcoRII	CCWGG	1 cut(s)	243
Esp3I	CGTCTC	1 cut(s)	494
FaeI	CATG	3 cut(s)	305, 341, 608
FaiI	YATR	8 cut(s)	294, 303, 339, 384, 431, 606, 623, 639
FaqI	GGGAC	1 cut(s)	123
FatI	CATG	3 cut(s)	301, 337, 604
FbaI	TGATCA	1 cut(s)	30
Fnu4HI	GCNGC	2 cut(s)	10, 159
FokI	GGATG	1 cut(s)	213
Fsp4HI	GCNGC	2 cut(s)	10, 159
FspBI	CTAG	3 cut(s)	347, 414, 647
GlaI	GCGC	1 cut(s)	313
GluI	GCNGC	2 cut(s)	10, 159
HaeIII	GGCC	2 cut(s)	38, 110
HapII	CCGG	5 cut(s)	47, 60, 114, 268, 396
HhaI	GCGC	1 cut(s)	314
Hin1II	CATG	3 cut(s)	305, 341, 608
Hin6I	GCGC	1 cut(s)	312
HinP1I	GCGC	1 cut(s)	312
HinfI	GANTC	2 cut(s)	455, 490
HpaII	CCGG	5 cut(s)	47, 60, 114, 268, 396
HphI	GGTGA	2 cut(s)	118, 454
Hpy166II	GTNNAC	3 cut(s)	94, 235, 241
Hpy188I	TCNGA	5 cut(s)	30, 478, 512, 531, 550
Hpy188III	TCNNGA	3 cut(s)	414, 424, 452
Hpy8I	GTNNAC	3 cut(s)	94, 235, 241
HpyAV	CCTTC	1 cut(s)	256
HpyCH4III	ACNGT	1 cut(s)	420
HpyCH4IV	ACGT	1 cut(s)	237
HpyCH4V	TGCA	6 cut(s)	301, 365, 463, 596, 630, 641
HpyF10VI	GCNNNNNNNGC	2 cut(s)	95, 298
HpyF3I	CTNAG	4 cut(s)	333, 516, 528, 666
HpySE526I	ACGT	1 cut(s)	237
Hsp92II	CATG	3 cut(s)	305, 341, 608
HspAI	GCGC	1 cut(s)	312
KroI	GCCGGC	1 cut(s)	46
KroNI	GCCGGC	1 cut(s)	48
Ksp22I	TGATCA	1 cut(s)	30
Kzo9I	GATC	3 cut(s)	30, 550, 580
LmnI	GCTCC	3 cut(s)	62, 289, 529
LweI	GCATC	1 cut(s)	516
MaeI	CTAG	3 cut(s)	347, 414, 647
MaeII	ACGT	1 cut(s)	237
MalI	GATC	3 cut(s)	32, 552, 582
MbiI	CCGCTC	2 cut(s)	9, 57
MboI	GATC	3 cut(s)	30, 550, 580
MboII	GAAGA	6 cut(s)	162, 165, 227, 260, 271, 321
MluCI	AATT	5 cut(s)	379, 406, 447, 616, 653
MlyI	GAGTC	2 cut(s)	449, 499
MnlI	CCTC	8 cut(s)	95, 121, 134, 173, 244, 382, 537, 571
MroNI	GCCGGC	1 cut(s)	46
MseI	TTAA	1 cut(s)	587
MspI	CCGG	5 cut(s)	47, 60, 114, 268, 396
MspR9I	CCNGG	3 cut(s)	245, 269, 397
Mva1269I	GAATGC	2 cut(s)	50, 227
MvaI	CCWGG	1 cut(s)	245
MwoI	GCNNNNNNNGC	2 cut(s)	95, 298
NaeI	GCCGGC	1 cut(s)	48
NciI	CCSGG	2 cut(s)	269, 397
NdeII	GATC	3 cut(s)	30, 550, 580
NgoMIV	GCCGGC	1 cut(s)	46
NlaIII	CATG	3 cut(s)	305, 341, 608
NlaIV	GGNNCC	1 cut(s)	139
NspI	RCATGY	2 cut(s)	305, 341
NspV	TTCGAA	1 cut(s)	257
PcsI	WCGNNNNNNNCGW	1 cut(s)	87
PctI	GAATGC	2 cut(s)	50, 227
PdiI	GCCGGC	1 cut(s)	48
PflFI	GACNNNGTC	1 cut(s)	77
PkrI	GCNGC	2 cut(s)	11, 160
Ple19I	CGATCG	1 cut(s)	583
PleI	GAGTC	2 cut(s)	449, 498
PpsI	GAGTC	2 cut(s)	449, 498
Ppu21I	YACGTR	1 cut(s)	238
Psp6I	CCWGG	1 cut(s)	243
PspGI	CCWGG	1 cut(s)	243
PspN4I	GGNNCC	1 cut(s)	139
PspPI	GGNCC	3 cut(s)	37, 137, 265
PstI	CTGCAG	1 cut(s)	632
PstNI	CAGNNNCTG	1 cut(s)	548
PsyI	GACNNNGTC	1 cut(s)	77
PvuI	CGATCG	1 cut(s)	583
RsaI	GTAC	2 cut(s)	224, 236
RsaNI	GTAC	2 cut(s)	223, 235
SaqAI	TTAA	1 cut(s)	587
SatI	GCNGC	2 cut(s)	10, 159
Sau3AI	GATC	3 cut(s)	30, 550, 580
Sau96I	GGNCC	3 cut(s)	37, 137, 265
SchI	GAGTC	2 cut(s)	449, 499
ScrFI	CCNGG	3 cut(s)	245, 269, 397
SetI	ASST	4 cut(s)	126, 240, 246, 267
SfaNI	GCATC	1 cut(s)	516
SfcI	CTRYAG	1 cut(s)	628
SfuI	TTCGAA	1 cut(s)	257
SinI	GGWCC	2 cut(s)	137, 265
SmlI	CTYRAG	1 cut(s)	469
SmoI	CTYRAG	1 cut(s)	469
SpeI	ACTAGT	1 cut(s)	646
Sse9I	AATT	5 cut(s)	379, 406, 447, 616, 653
SsiI	CCGC	5 cut(s)	9, 55, 129, 159, 402
SspMI	CTAG	3 cut(s)	347, 414, 647
StyD4I	CCNGG	3 cut(s)	243, 267, 395
TaaI	ACNGT	1 cut(s)	420
TaiI	ACGT	1 cut(s)	240
TaqI	TCGA	5 cut(s)	213, 257, 342, 453, 583
TasI	AATT	5 cut(s)	379, 406, 447, 616, 653
TauI	GCSGC	2 cut(s)	12, 161
Tru1I	TTAA	1 cut(s)	587
Tru9I	TTAA	1 cut(s)	587
TscAI	CASTG	2 cut(s)	24, 139
TspDTI	ATGAA	2 cut(s)	198, 562
TspRI	CASTG	2 cut(s)	24, 139
Tth111I	GACNNNGTC	1 cut(s)	77
VpaK11BI	GGWCC	2 cut(s)	137, 265
XagI	CCTNNNNNAGG	1 cut(s)	248
XapI	RAATTY	1 cut(s)	447
XbaI	TCTAGA	1 cut(s)	413
XceI	RCATGY	2 cut(s)	305, 341
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XspI	CTAG	3 cut(s)	347, 414, 647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.