MD12G1214600.v1.1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0020976
Physical Location & Seq
Reverse (-)
29318666 .. 29318845
180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1214600.v1.1.491

Sequence Viewer

Length: 180 bp
ATGGTGAAGATGCTTCAGACCAAGCCCATGGTTGTGGAGACATTTTCTGAGTACCCTCCATTAGGTCACTTTGTTGTTCGTGGCATGCACCAGACTGTTGCTATTGGTGTTATCAAGAGTGTTGAGAAGAAGGATCCCAGCGGTGCCAAGGTCACCAAGTCTGCTGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

60

Amino Acids

6.47

Weight (kDa)

10.08

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-eEF1A_C PF22594 2 - 39 1.4e-10 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35550
malus_domestica MD12G1214600.v1.1 MD16G1024200.v1.1 MD17G1192800.v1.1
pyrus_communis pycom04g00880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 143
AciI CCGC 1 cut(s) 141
AclWI GGATC 2 cut(s) 128, 141
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 128, 141
ApeKI GCWGC 1 cut(s) 164
AsuHPI GGTGA 2 cut(s) 16, 145
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 32
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 135, 145
BsaJI CCNNGG 2 cut(s) 27, 147
Bsc4I CCNNNNNNNGG 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 27, 147
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 1 cut(s) 39
BseXI GCAGC 1 cut(s) 151
BseYI CCCAGC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 133
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 135, 145
BspPI GGATC 2 cut(s) 128, 141
BspT107I GGYRCC 1 cut(s) 143
BssECI CCNNGG 2 cut(s) 27, 147
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 2 cut(s) 27, 147
Bst4CI ACNGT 1 cut(s) 97
BstC8I GCNNGC 1 cut(s) 86
BstDEI CTNAG 1 cut(s) 48
BstDSI CCRYGG 1 cut(s) 27
BstEII GGTNACC 1 cut(s) 151
BstENI CCTNNNNNAGG 1 cut(s) 60
BstKTI GATC 1 cut(s) 136
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 1 cut(s) 133
BstNSI RCATGY 1 cut(s) 88
BstPI GGTNACC 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 2 cut(s) 28, 34
BstYI RGATCY 1 cut(s) 133
BtgI CCRYGG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 52
CviAII CATG 2 cut(s) 28, 85
CviJI RGCY 1 cut(s) 25
CviKI_1 RGCY 1 cut(s) 25
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 1 cut(s) 48
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 27, 147
Eco91I GGTNACC 1 cut(s) 151
EcoNI CCTNNNNNAGG 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 151
EcoT14I CCWWGG 2 cut(s) 27, 147
ErhI CCWWGG 2 cut(s) 27, 147
FaeI CATG 2 cut(s) 31, 88
FaiI YATR 2 cut(s) 29, 86
FatI CATG 2 cut(s) 27, 84
Fnu4HI GCNGC 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 165
GsaI CCCAGC 1 cut(s) 141
Hin1II CATG 2 cut(s) 31, 88
HphI GGTGA 2 cut(s) 16, 145
Hpy188I TCNGA 2 cut(s) 18, 49
Hpy188III TCNNGA 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 124
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 88
HpyF3I CTNAG 1 cut(s) 48
Hsp92II CATG 2 cut(s) 31, 88
Kzo9I GATC 1 cut(s) 133
LpnPI CCDG 2 cut(s) 104, 151
Lsp1109I GCAGC 1 cut(s) 151
MaeIII GTNAC 2 cut(s) 65, 151
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 2 cut(s) 19, 139
MflI RGATCY 1 cut(s) 133
MnlI CCTC 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 27
NdeII GATC 1 cut(s) 133
NlaIII CATG 2 cut(s) 31, 88
NlaIV GGNNCC 2 cut(s) 135, 145
NmuCI GTSAC 2 cut(s) 65, 151
NspI RCATGY 1 cut(s) 88
PaeI GCATGC 1 cut(s) 88
PkrI GCNGC 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 135, 145
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 32
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 133
SetI ASST 2 cut(s) 67, 153
SgeI CNNG 9 cut(s) 34, 40, 92, 97, 103, 127, 150, 160, 169
SmiMI CAYNNNNRTG 1 cut(s) 32
SphI GCATGC 1 cut(s) 88
SsiI CCGC 1 cut(s) 141
StyI CCWWGG 2 cut(s) 27, 147
TaaI ACNGT 1 cut(s) 97
TseFI GTSAC 2 cut(s) 65, 151
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 2 cut(s) 65, 151
XagI CCTNNNNNAGG 1 cut(s) 60
XceI RCATGY 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.