MD16G1024200.v1.1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020976
Physical Location & Seq
Reverse (-)
1738362 .. 1738577
216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1024200.v1.1.491

Sequence Viewer

Length: 216 bp
ATGGAGACAAAGTTCTTGAAGAACGGAGATGCAGTTATGGTGAAGATGCTTCCTACCAAGCCCATGGTTGTGGTGACATTTTCTGAGTACCATCCATTAGGTTGCTTTGTTGTTTGTGGCATGCGCCAGATTGTTGCTTTTGGTGTTATCAAGAGTGTTGAGAAGAAGGATCCCATCGATGCCAAGGTCACCAAGTCTGCTGCTAAGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

72

Amino Acids

7.85

Weight (kDa)

9.7

Isoelectric Point (pI)

12.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP-eEF1A_C PF22594 3 - 51 2.8e-11 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35550
malus_domestica MD12G1214600.v1.1 MD16G1024200.v1.1 MD17G1192800.v1.1
pyrus_communis pycom04g00880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 164, 177
AfaI GTAC 1 cut(s) 89
AgsI TTSAA 1 cut(s) 19
AloI GAACNNNNNNTCC 1 cut(s) 28
AlwI GGATC 2 cut(s) 164, 177
ApeKI GCWGC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 126
AsuHPI GGTGA 3 cut(s) 52, 85, 181
BamHI GGATCC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 187
BccI CCATC 2 cut(s) 99, 182
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 171
BmsI GCATC 3 cut(s) 19, 36, 169
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 63, 183
BsaXI ACNNNNNCTCC 3 cut(s) 18, 26, 48
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 2 cut(s) 63, 183
BseGI GGATG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 177
Bsp143I GATC 1 cut(s) 169
Bsp19I CCATGG 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 76
BspDI ATCGAT 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 171
BspPI GGATC 2 cut(s) 164, 177
BssECI CCNNGG 2 cut(s) 63, 183
BssMI GATC 1 cut(s) 169
BssT1I CCWWGG 2 cut(s) 63, 183
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 2 cut(s) 84, 204
BstDSI CCRYGG 1 cut(s) 63
BstEII GGTNACC 1 cut(s) 187
BstF5I GGATG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 1 cut(s) 172
BstMBI GATC 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 124
BstPI GGTNACC 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 169
BstXI CCANNNNNNTGG 2 cut(s) 64, 70
BstYI RGATCY 1 cut(s) 169
Bsu15I ATCGAT 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 91
Cac8I GCNNGC 1 cut(s) 122
CfoI GCGC 1 cut(s) 126
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 1 cut(s) 88
CviAII CATG 2 cut(s) 64, 121
CviJI RGCY 1 cut(s) 61
CviKI_1 RGCY 1 cut(s) 61
CviQI GTAC 1 cut(s) 88
DdeI CTNAG 2 cut(s) 84, 204
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 63, 183
Eco91I GGTNACC 1 cut(s) 187
EcoO65I GGTNACC 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 63, 183
ErhI CCWWGG 2 cut(s) 63, 183
FaeI CATG 2 cut(s) 67, 124
FaiI YATR 3 cut(s) 38, 65, 122
FatI CATG 2 cut(s) 63, 120
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 1 cut(s) 125
GluI GCNGC 1 cut(s) 201
HhaI GCGC 1 cut(s) 126
Hin1II CATG 2 cut(s) 67, 124
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HphI GGTGA 3 cut(s) 52, 85, 181
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 2 cut(s) 16, 151
HpyAV CCTTC 1 cut(s) 160
HpyCH4V TGCA 1 cut(s) 32
HpyF3I CTNAG 2 cut(s) 84, 204
Hsp92II CATG 2 cut(s) 67, 124
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 169
LpnPI CCDG 1 cut(s) 140
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 3 cut(s) 19, 36, 169
MaeIII GTNAC 2 cut(s) 73, 187
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 3 cut(s) 31, 55, 175
MflI RGATCY 1 cut(s) 169
MslI CAYNNNNRTG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 63
NdeII GATC 1 cut(s) 169
NlaIII CATG 2 cut(s) 67, 124
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 2 cut(s) 73, 187
NspI RCATGY 1 cut(s) 124
PaeI GCATGC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
RseI CAYNNNNRTG 1 cut(s) 68
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 1 cut(s) 169
SetI ASST 2 cut(s) 103, 189
SfaNI GCATC 3 cut(s) 19, 36, 169
SgeI CNNG 8 cut(s) 28, 70, 76, 133, 139, 163, 196, 205
SmiMI CAYNNNNRTG 1 cut(s) 68
SphI GCATGC 1 cut(s) 124
StyI CCWWGG 2 cut(s) 63, 183
TaqI TCGA 1 cut(s) 177
TseFI GTSAC 2 cut(s) 73, 187
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 2 cut(s) 73, 187
TspGWI ACGGA 1 cut(s) 39
XceI RCATGY 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.