MD12G1217200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017682
Physical Location & Seq
Reverse (-)
29468527 .. 29470495
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1217200.v1.1.491

Sequence Viewer

Length: 579 bp
ATGTTGCTCCTCGGTCTCAGACAAAAGCAGAAACCACACCGTTACCTCAGACCAGTTCAACAAACGTCTCCGTTTAACCCTTCATCTCCTTCCTTCCCACTGCTATACCCTGAAAGGCAGCAAGAAAGAAACCCTAGCTTCTGCCCGTATTTTCCCGGAAACCAAACAGGAAGAAATGCCTCCAAGAAATTCACCACTTCCGGTCAGACTTTCATGCCAACCAATTTCAGAAGCAGTTGCAAAGCCTTCCGCCACTCCAGCAGTACAAATAAAGCCATACCATCTTCGAATCGGAGTTGTCAAGATCATCTTCGGACGCCCTCAAAACCGCTTTTGAAAATTATGTCTTGTTATAGGAATGTACACTCTTGTAGCCACACGATCATGTTTGGTTATTGGGTTGGTCCTGATATTGATGATGGATGGGGCTATGTGGAAGCTTTTATTAATCCAATCACTAGAGTATTGTTCAATATTCCATGTTTTTTCTCCTTATGTTTTGGGATATTTCATAGATTCATTCAAGACACACTTTTTGTATGTGTTAACAACGTCCAATTTGTCGATGAAAACGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

22.1

Weight (kDa)

9.56

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017682)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g06210
malus_domestica MD12G1217200.v1.1
prunus_persica Prupe.6G323500_v2.0.a1
pyrus_communis pycom04g17980 pycom12g20270
rosa_chinensis RchiOBHm_Chr3g0455231
rosa_laevigata RLG00000025375
rosa_multiflora Rmu_sc0002169.1_g000033
rosa_roxburghii Rroxscaffold_6G00424450
rosa_samantha Rh3AG065400 Rh3CG066000 Rh3DG067600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 575
AciI CCGC 2 cut(s) 250, 329
AcsI RAATTY 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 2 cut(s) 265, 363
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 4 cut(s) 59, 337, 472, 524
AluBI AGCT 2 cut(s) 138, 440
AluI AGCT 2 cut(s) 138, 440
Alw26I GTCTC 2 cut(s) 20, 72
ApeKI GCWGC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 188
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AseI ATTAAT 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 184
AsuII TTCGAA 1 cut(s) 287
AvaII GGWCC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 130
BccI CCATC 3 cut(s) 289, 413, 417
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 20, 72
BfaI CTAG 2 cut(s) 135, 459
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 404
BmrFI CCNGG 1 cut(s) 156
BpmI CTGGAG 1 cut(s) 241
Bpu14I TTCGAA 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 156
BsaHI GRCGYC 1 cut(s) 317
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 53
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 31, 61
BseNI ACTGG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 130
Bsh1236I CGCG 1 cut(s) 575
BsiSI CCGG 2 cut(s) 156, 201
BsmAI GTCTC 2 cut(s) 20, 72
BsmBI CGTCTC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 2 cut(s) 304, 381
BspACI CCGC 2 cut(s) 250, 329
BspCNI CTCAG 2 cut(s) 30, 60
BspFNI CGCG 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 304, 381
BssNI GRCGYC 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 287
BstDEI CTNAG 2 cut(s) 17, 47
BstF5I GGATG 1 cut(s) 428
BstFNI CGCG 1 cut(s) 575
BstKTI GATC 2 cut(s) 307, 384
BstMAI GTCTC 2 cut(s) 20, 72
BstMBI GATC 2 cut(s) 304, 381
BstMWI GCNNNNNNNGC 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 154
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 428
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 404
CseI GACGC 1 cut(s) 325
Csp6I GTAC 2 cut(s) 264, 362
CviAII CATG 3 cut(s) 214, 385, 480
CviJI RGCY 6 cut(s) 138, 245, 275, 375, 429, 440
CviKI_1 RGCY 6 cut(s) 138, 245, 275, 375, 429, 440
CviQI GTAC 2 cut(s) 264, 362
DdeI CTNAG 2 cut(s) 17, 47
DpnI GATC 2 cut(s) 306, 383
DpnII GATC 2 cut(s) 304, 381
EciI GGCGGA 1 cut(s) 239
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 404
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 3 cut(s) 217, 388, 483
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 516, 548
FatI CATG 3 cut(s) 213, 384, 479
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 1 cut(s) 435
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 2 cut(s) 135, 459
GluI GCNGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 241
HapII CCGG 2 cut(s) 156, 201
HgaI GACGC 1 cut(s) 325
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 3 cut(s) 217, 388, 483
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 2 cut(s) 289, 516
HpaI GTTAAC 1 cut(s) 547
HpaII CCGG 2 cut(s) 156, 201
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 2 cut(s) 364, 547
Hpy188I TCNGA 6 cut(s) 20, 50, 207, 230, 294, 315
Hpy188III TCNNGA 3 cut(s) 302, 407, 524
Hpy8I GTNNAC 2 cut(s) 364, 547
HpyAV CCTTC 4 cut(s) 90, 99, 103, 256
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4IV ACGT 2 cut(s) 65, 552
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 1 cut(s) 258
HpyF3I CTNAG 2 cut(s) 17, 47
HpySE526I ACGT 2 cut(s) 65, 552
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 217, 388, 483
KspAI GTTAAC 1 cut(s) 547
Kzo9I GATC 2 cut(s) 304, 381
LmnI GCTCC 1 cut(s) 12
LpnPI CCDG 7 cut(s) 66, 123, 153, 169, 214, 271, 420
Lsp1109I GCAGC 1 cut(s) 130
MaeI CTAG 2 cut(s) 135, 459
MaeII ACGT 2 cut(s) 65, 552
MaeIII GTNAC 1 cut(s) 41
MalI GATC 2 cut(s) 306, 383
MboI GATC 2 cut(s) 304, 381
MboII GAAGA 3 cut(s) 183, 276, 302
MluCI AATT 4 cut(s) 188, 223, 339, 557
MluI ACGCGT 1 cut(s) 573
MnlI CCTC 4 cut(s) 20, 56, 190, 331
MseI TTAA 3 cut(s) 75, 447, 546
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 2 cut(s) 156, 201
MspR9I CCNGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 1 cut(s) 258
NciI CCSGG 1 cut(s) 156
NdeII GATC 2 cut(s) 304, 381
NlaIII CATG 3 cut(s) 217, 388, 483
NspV TTCGAA 1 cut(s) 287
PfeI GAWTC 2 cut(s) 289, 516
PfoI TCCNGGA 1 cut(s) 154
PkrI GCNGC 1 cut(s) 120
PshBI ATTAAT 1 cut(s) 447
PspPI GGNCC 1 cut(s) 404
RsaI GTAC 2 cut(s) 265, 363
RsaNI GTAC 2 cut(s) 264, 362
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 3 cut(s) 75, 447, 546
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 2 cut(s) 304, 381
Sau96I GGNCC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 156
SetI ASST 5 cut(s) 48, 68, 140, 442, 555
SfuI TTCGAA 1 cut(s) 287
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 383
Sse9I AATT 4 cut(s) 188, 223, 339, 557
SsiI CCGC 2 cut(s) 250, 329
SspI AATATT 1 cut(s) 475
SspMI CTAG 2 cut(s) 135, 459
StyD4I CCNGG 1 cut(s) 154
TaaI ACNGT 1 cut(s) 41
TaiI ACGT 2 cut(s) 68, 555
TaqI TCGA 2 cut(s) 287, 564
TasI AATT 4 cut(s) 188, 223, 339, 557
TatI WGTACW 2 cut(s) 263, 361
TfiI GAWTC 2 cut(s) 289, 516
Tru1I TTAA 3 cut(s) 75, 447, 546
Tru9I TTAA 3 cut(s) 75, 447, 546
TscAI CASTG 1 cut(s) 105
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 4 cut(s) 72, 202, 500, 508
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 404
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 1 cut(s) 188
XspI CTAG 2 cut(s) 135, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.