Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015312

Physical Location & Seq

Genomic Coordinates Forward (+)

927291 .. 930799

Gene Structure 3509 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1014800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3432 bp

ATGAAACTTGTTTCATCAATGGAGAACTTCAATCCAGCTCAGTTTTTTCTTCATCTTTCTGCAGTACTTGTATTGGTAGGACTTCACCTTGTTCCTGTTTCCACAGCTGACCAACAAGTCACTCCATCGATCAAAACCGACGCAGTTTCTCTTCTCAACTTCAAAAAGATGATTAACAAGGACCCGAATGGTGTGTTGTCCGGTTGGAAGCTCGGCACCAGTCCATGCAGCTGGTATGGAGTTACATGCTCCTTGAACCGGGCAACTCAGCTCGATCTTACCAGTTGTTCTCTTGTTGGTACAATCTCTTTTGAACCCTTGGCCTCTCTAGACATGCTTTCTGTCTTAAATCTGCCTTCCAATAAATTCAGTGTAAACGCTACCTCTTTGCTTCAACTCCCATATGCTTTGAAACAACTCGATTTATCGTTCAACGCACTTTTCGGTGTTCTTCCTGAGGGTGTTTCCTCAAAATGTCCAAATCTTGTCTCTGTGAATCTTGCTTTTAACAACTTAACCGGCCCTCCACCCAAAGATCTCTTGTTGCATTCCGACAAACTTCAAACTCTCGACATCTCTTACAACAATCTAACCGGGTCAATTTCTGGTCTGATAATTGAGAAATATTCTTGCCCTTCTTTGTTGCAGCTTGATTTGTCAGGAAACCGTATAACGGGTTCCATTCCTATGTCCTTGGCAAACTGCACCAGTCTCAAAACTATGAGTTTATCGACCAACAACGTGACAGGCGAAATCCCAAGATCGTTCGGGCAACTCCGCAGTCTACAGAGATTGGATCTTTCTCACAATCAGATAACCGGTTGGATACCTCCTGAATTAGGAAATGCATGCGGTTCGCTTGCCGAACTCAAGCTTTCGTTTAACAACATTACTGGCCCAATTCCAGCCTCTTTCTCCTCGTGTTCTTTGCTGGAGGCTCTCGATCTCTCCAACAACAACTTAACAGGCCCCCTGCCAGATTCTATCTTTCAGAACCTCACCTCCTTACAGAGCTTGGTGCTGAGGAATAACATCATCACTGGATCACTTCCGGGCTCCATATCAGCTTGCAGGAGCCTACAGGTTATAGACTTGAGCTCTAATAAAATATCTGGCGTCATCCCACCAGATATATGTCCCGGGGCCTCCTCACTTGAGGAGCTGAGAATGCCGGACAACCTCATTGTAGGCGAAATTCCTGCTCAACTATCACAATGTTCTCAGCTGAAGACGATCGATTTTAGTTTGAACTATCTCAACGGCTCAATTCCAGCTGAGCTTGGGAAGCTGGAGAATTTGCAGCAGCTAATAGCATGGTACAATGGCTTAGAGGGGAAAATCCCACCAGACTTGGGAAAGTGCAAGAATCTCAAGGATATTATTCTCAACAATAACCATCTGAGTGGTGAAATCCCAGTTGAATTGTTCAGCTGCAGCAATCTTGAATGGATATCACTCACAAGCAATAAACTCAGTGGTGAAGTCCCAAGGGAATTTGGCCTTTTGACACGACTGGCGGTTCTACAACTTGGGAACAATAGCTTGAGTGGCAAGATACCAGGAGAGCTTGGAAATTGCAGCAGTTTGGTTTGGTTGGATTTGAACAGCAACAAACTCACCGGAGAGATCCCACCTCGACTTGGGAGGCAGATCGGGGCCAAAGCACTGAGTGGAATTCTCTCCGGCAATACTTTGGTGTTTGTACGGAATGTGGGGAACTCTTGTAAAGGAGTGGGAGGCTTATTAGAGTTTGCGGGAATCCGACCTGAAAGGCTTCAACAGGACCCAACATTGAGGACTTGTGATTTCGCCAGATTGTACTCTGGCCCTGTCCTGAGTCTCTTTACAAAATACCAAACACTGGAGTATCTTGATCTATCTTACAATCAGCTTCGCGGGAAAATCCCGGAAGAAATGGGGGACATGATTGCATTGCAAGTTCTTGAGTTATCCCACAACCAGTTATCAGGTGAGATTCCTGCATCACTTGGCCAGCTCAAAGATTTAGGGGTGTTTGATGCATCACACAACAGACTGCAGGGTCACATCCCTGATTCATTGGAGAACCTATCTTTCTTGGTGCAAATTGATTTGTCCAACAATGAATTAACTGGCGAAATTCCCTCGAGGGGTCAGCTGAGTACGCTTCCTGCTACCCAGTATGCTAACAACCCTGGACTCTGTGGAGTCCCATTGCCTGATTGCCAGAGCAGCAATGACCAACCAGACACCACTCCAAGCGATCAGGATGAAGGCAAACGAAGCCGAAGGCCATCAGTTGCTTCATGGGCTAACAGCATTGTTGTGGGGGTTCTGATTTCTCTTGCTTCTGTCTGTGTTCTGGTTGTGTGGGCAATTGCAATGCGGACGAGGCGAAAGGAAGCGAAGGAGGTGAAGATGCTTAATCGCTTGCAAGCATCCCATGCGGCATGGAAGATTGACAAAGAGAAAGAACCCTTGAGTATTAATGTTGCCACTTTCCAAAGACAGTTGAGGAAGCTCAAGTTCTCCCAACTCATTGAGGCAACCAATGGCTTCTCGGCAGATAGTCTTATAGGGTGTGGAGGTTTTGGGGAAGTGTTCAAGGCAACGCTGAAAGATGGATCAAGTGTTGCAATCAAGAAACTTATACGGCTGAGCTGCCAAGGTGACCGTGAATTCATGGCCGAGATGGAAACTCTGGGGAAGATCAAGCATAGGAATCTCGTGCCTTTATTGGGTTATTGTAAAATTGGTGAAGAGAGGCTGCTAGTATATGAATTCATGGAGTACGGAAGCCTCGAAGAAATGCTCCATGGAAGAACAAAGACGCGTGACAGGCGGATGCTAACATGGGAGGAAAGAAAAAAGATTGCAAGAGGTGCAGCGAAAGGACTGTGTTTCCTCCACCACAATTGCATCCCTCACATCATACACAGAGACATGAAGTCAAGCAATGTATTGTTGGACAGTGAATTGGAAGCAAGAGTTTCTGATTTCGGAATGGCAAGGCTCATAAGCGCTCTTGACACACACTTGAGTGTAAGCACTCTTGCAGGCACTCCTGGTTATGTTCCACCAGAATACTACCAGAGTTTCCGCTGCACTGCCAAGGGTGACGTCTACTCATTTGGGGTTGTGCTCTTGGAGCTTGTGACCGGAAAACGCCCAACAGACAAGGATGATTTCGGGGACACTAACTTGGTCGGATGGGCGAAGATGAAGGTGAGAGAAGGGAAACAGATGGAAGTGATAGACCAAGAGTTACTTTCGGTAACTAAAGGAACTGATGAAGCAGAAGCAGAAGAAGTGAAAGAGATGGTGAGGTATTTAGAGGTGACACTTCAATGTGTTGACGACTTCCCATCAAAGAGGCCTAACATGTTGCAGGTGGTGGCCATGCTGAGAGAGCTGATATCTGGATCAGCATGTGGAAGTAGTAATAGCGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1144

Amino Acids

124.65

Weight (kDa)

6.22

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	46 - 84	3.9e-12	Leucine rich repeat N-terminal domain
LRR_8	PF13855	212 - 272	5.6e-10	Leucine rich repeat
LRR_14	PF23598	303 - 438	3.7e-07	Leucine-rich repeat region
LRR_8	PF13855	325 - 370	1e-07	Leucine rich repeat
LRR_4	PF12799	334 - 371	7e-06	Leucine Rich repeats (2 copies)
Island	PF20141	556 - 616	9.2e-19	Brassinosteroid receptor island domain
LRR_14	PF23598	612 - 699	5.4e-07	Leucine-rich repeat region
LRR_8	PF13855	614 - 655	2.9e-06	Leucine rich repeat
Pkinase	PF00069	848 - 1125	3.8e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	849 - 1127	9e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0015312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01950
fragaria_vesca	FvH4_5g27210
malus_domestica	MD13G1014800.v1.1
prunus_persica	Prupe.1G338800_v2.0.a1
pyrus_communis	pycom13g01260 pycom16g01160
rosa_chinensis	RchiOBHm_Chr7g0218311
rosa_laevigata	RLG00000002379
rosa_multiflora	Rmu_sc0009526.1_g000007
rosa_roxburghii	Rroxscaffold_3G00241060
rosa_rugosa	Rorug07G0176700
rosa_samantha	Rh7AG318000 Rh7BG309700 Rh7CG334800 Rh7DG317000
rosa_wichuraiana	Rw7G027020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3358
AasI	GACNNNNNNGTC	2 cut(s)	116, 2040
AatII	GACGTC	1 cut(s)	3102
AbsI	CCTCGAGG	1 cut(s)	2125
Acc36I	ACCTGC	1 cut(s)	3358
AccB1I	GGYRCC	1 cut(s)	215
AccB7I	CCANNNNNTGG	1 cut(s)	1861
AccI	GTMKAC	2 cut(s)	784, 3102
AccII	CGCG	2 cut(s)	1896, 2812
AciI	CCGC	9 cut(s)	778, 852, 1517, 1754, 1896, 2365, 2426, 2821, 3079
AclWI	GGATC	5 cut(s)	804, 1051, 1621, 2611, 3409
AcoI	YGGCCR	3 cut(s)	1990, 2664, 3375
AcsI	RAATTY	8 cut(s)	365, 1194, 1294, 1493, 1674, 2118, 2657, 2759
AcuI	CTGAAG	1 cut(s)	1247
AcyI	GRCGYC	2 cut(s)	1116, 3099
AdeI	CACNNNGTG	1 cut(s)	1670
AfaI	GTAC	7 cut(s)	66, 301, 1319, 1704, 1820, 2143, 2771
AfeI	AGCGCT	2 cut(s)	3001, 3427
AfiI	CCNNNNNNNGG	8 cut(s)	258, 673, 839, 1352, 1640, 1861, 2129, 2717
AflIII	ACRYGT	2 cut(s)	2810, 3360
AgeI	ACCGGT	1 cut(s)	818
AhdI	GACNNNNNGTC	1 cut(s)	2926
AjnI	CCWGG	3 cut(s)	1558, 2173, 3043
AleI	CACNNNNGTG	1 cut(s)	3018
AloI	GAACNNNNNNTCC	2 cut(s)	986, 1018
Alw21I	GWGCWC	2 cut(s)	1100, 3123
Alw26I	GTCTC	4 cut(s)	493, 716, 1844, 2913
AlwI	GGATC	5 cut(s)	804, 1051, 1621, 2611, 3409
Ama87I	CYCGRG	2 cut(s)	1139, 2125
Aor51HI	AGCGCT	2 cut(s)	3001, 3427
ApoI	RAATTY	8 cut(s)	365, 1194, 1294, 1493, 1674, 2118, 2657, 2759
AseI	ATTAAT	1 cut(s)	2466
AsiGI	ACCGGT	1 cut(s)	818
Asp700I	GAANNNNTTC	1 cut(s)	1773
AspLEI	GCGC	2 cut(s)	3002, 3428
AspS9I	GGNCC	8 cut(s)	181, 521, 896, 968, 1143, 1656, 1783, 1826
AsuC2I	CCSGG	6 cut(s)	260, 595, 1053, 1140, 1141, 1907
AvaI	CYCGRG	2 cut(s)	1139, 2125
AvaII	GGWCC	2 cut(s)	181, 1783
AxyI	CCTNAGG	1 cut(s)	456
BaeI	ACNNNNGTAYC	2 cut(s)	1850, 1883
BalI	TGGCCA	2 cut(s)	1992, 3377
BanI	GGYRCC	1 cut(s)	215
BanII	GRGCYC	2 cut(s)	1058, 1100
BauI	CACGAG	2 cut(s)	919, 2705
BbsI	GAAGAC	1 cut(s)	1235
Bbv12I	GWGCWC	2 cut(s)	1100, 3123
BbvCI	CCTCAGC	1 cut(s)	1022
BccI	CCATC	9 cut(s)	133, 1404, 2281, 2594, 2665, 3183, 3217, 3292, 3352
BceAI	ACGGC	2 cut(s)	1276, 2648
BcgI	CGANNNNNNTGC	4 cut(s)	837, 871, 1181, 1215
BciT130I	CCWGG	3 cut(s)	1560, 2175, 3045
BciVI	GTATCC	1 cut(s)	819
BcnI	CCSGG	6 cut(s)	260, 595, 1053, 1140, 1141, 1907
BcoDI	GTCTC	4 cut(s)	493, 716, 1844, 2913
BfaI	CTAG	2 cut(s)	329, 2750
BfmI	CTRYAG	5 cut(s)	60, 785, 1079, 1432, 2036
BfoI	RGCGCY	2 cut(s)	3003, 3429
BfuAI	ACCTGC	1 cut(s)	3358
BfuI	GTATCC	1 cut(s)	819
BglII	AGATCT	1 cut(s)	535
BlpI	GCTNAGC	2 cut(s)	1275, 2636
BmcAI	AGTACT	1 cut(s)	66
Bme1390I	CCNGG	9 cut(s)	260, 595, 1053, 1140, 1141, 1560, 1907, 2175, 3045
Bme18I	GGWCC	2 cut(s)	181, 1783
BmeRI	GACNNNNNGTC	1 cut(s)	2926
BmeT110I	CYCGRG	2 cut(s)	1139, 2125
BmgT120I	GGNCC	8 cut(s)	181, 521, 896, 968, 1143, 1656, 1783, 1826
BmiI	GGNNCC	9 cut(s)	183, 217, 679, 970, 1057, 1076, 1144, 1657, 1785
BmrFI	CCNGG	9 cut(s)	260, 595, 1053, 1140, 1141, 1560, 1907, 2175, 3045
BmrI	ACTGGG	2 cut(s)	1409, 2152
BmsI	GCATC	7 cut(s)	1991, 2008, 2030, 2388, 2426, 2814, 2907
BmuI	ACTGGG	2 cut(s)	1409, 2152
BpiI	GAAGAC	1 cut(s)	1235
BpmI	CTGGAG	3 cut(s)	953, 1310, 1883
Bpu10I	CCTNAGC	1 cut(s)	1022
Bpu1102I	GCTNAGC	2 cut(s)	1275, 2636
BpuEI	CTTGAG	9 cut(s)	854, 1114, 1175, 1355, 1564, 1964, 2479, 2486, 3037
BpuMI	CCSGG	6 cut(s)	260, 595, 1053, 1140, 1141, 1907
Bsa29I	ATCGAT	2 cut(s)	128, 1236
BsaHI	GRCGYC	2 cut(s)	1116, 3099
BsaJI	CCNNGG	9 cut(s)	318, 693, 1139, 1140, 1487, 2173, 2644, 2794, 3090
BsaWI	WCCGGW	4 cut(s)	200, 818, 1619, 3137
BsaXI	ACNNNNNCTCC	4 cut(s)	508, 538, 986, 1016
Bsc4I	CCNNNNNNNGG	8 cut(s)	258, 673, 839, 1352, 1640, 1861, 2129, 2717
Bse118I	RCCGGY	2 cut(s)	518, 818
Bse21I	CCTNAGG	1 cut(s)	456
Bse3DI	GCAATG	5 cut(s)	1931, 2192, 2221, 2367, 2941
BseBI	CCWGG	3 cut(s)	1560, 2175, 3045
BseCI	ATCGAT	2 cut(s)	128, 1236
BseDI	CCNNGG	9 cut(s)	318, 693, 1139, 1140, 1487, 2173, 2644, 2794, 3090
BseGI	GGATG	8 cut(s)	1119, 2046, 2254, 2417, 2829, 2898, 3166, 3194
BseLI	CCNNNNNNNGG	8 cut(s)	258, 673, 839, 1352, 1640, 1861, 2129, 2717
BseMI	GCAATG	5 cut(s)	1931, 2192, 2221, 2367, 2941
BseRI	GAGGAG	3 cut(s)	907, 1138, 1172
BsgI	GTGCAG	3 cut(s)	688, 2883, 3067
Bsh1236I	CGCG	2 cut(s)	1896, 2812
Bsh1285I	CGRYCG	1 cut(s)	1236
BshNI	GGYRCC	1 cut(s)	215
BshTI	ACCGGT	1 cut(s)	818
BshVI	ATCGAT	2 cut(s)	128, 1236
BsiEI	CGRYCG	1 cut(s)	1236
BsiHKAI	GWGCWC	2 cut(s)	1100, 3123
BsiHKCI	CYCGRG	2 cut(s)	1139, 2125
BslFI	GGGAC	5 cut(s)	1122, 1469, 1934, 2174, 3185
BslI	CCNNNNNNNGG	8 cut(s)	258, 673, 839, 1352, 1640, 1861, 2129, 2717
BsmAI	GTCTC	4 cut(s)	493, 716, 1844, 2913
BsmFI	GGGAC	5 cut(s)	1122, 1469, 1934, 2174, 3185
BsmI	GAATGC	2 cut(s)	547, 1173
BsoBI	CYCGRG	2 cut(s)	1139, 2125
Bsp1286I	GDGCHC	3 cut(s)	1058, 1100, 3123
Bsp1720I	GCTNAGC	2 cut(s)	1275, 2636
Bsp19I	CCATGG	1 cut(s)	2794
BspACI	CCGC	9 cut(s)	778, 852, 1517, 1754, 1896, 2365, 2426, 2821, 3079
BspDI	ATCGAT	2 cut(s)	128, 1236
BspFNI	CGCG	2 cut(s)	1896, 2812
BspLI	GGNNCC	9 cut(s)	183, 217, 679, 970, 1057, 1076, 1144, 1657, 1785
BspMAI	CTGCAG	3 cut(s)	64, 1436, 2040
BspMI	ACCTGC	1 cut(s)	3358
BspPI	GGATC	5 cut(s)	804, 1051, 1621, 2611, 3409
BspT107I	GGYRCC	1 cut(s)	215
BsrDI	GCAATG	5 cut(s)	1931, 2192, 2221, 2367, 2941
BsrFI	RCCGGY	2 cut(s)	518, 818
BssAI	RCCGGY	2 cut(s)	518, 818
BssECI	CCNNGG	9 cut(s)	318, 693, 1139, 1140, 1487, 2173, 2644, 2794, 3090
BssNI	GRCGYC	2 cut(s)	1116, 3099
BssSI	CACGAG	2 cut(s)	919, 2705
BssT1I	CCWWGG	6 cut(s)	318, 693, 1487, 2644, 2794, 3090
Bst2BI	CACGAG	2 cut(s)	919, 2705
Bst2UI	CCWGG	3 cut(s)	1560, 2175, 3045
Bst4CI	ACNGT	5 cut(s)	668, 2490, 2654, 2877, 2951
Bst6I	CTCTTC	2 cut(s)	156, 2733
BstACI	GRCGYC	2 cut(s)	1116, 3099
BstAPI	GCANNNNNTGC	2 cut(s)	2423, 2861
BstC8I	GCNNGC	7 cut(s)	850, 861, 1069, 1994, 2411, 2415, 3037
BstDSI	CCRYGG	1 cut(s)	2794
BstEII	GGTNACC	1 cut(s)	2648
BstENI	CCTNNNNNAGG	1 cut(s)	837
BstF5I	GGATG	8 cut(s)	1119, 2046, 2254, 2417, 2829, 2898, 3166, 3194
BstFNI	CGCG	2 cut(s)	1896, 2812
BstH2I	RGCGCY	2 cut(s)	3003, 3429
BstHHI	GCGC	2 cut(s)	3002, 3428
BstMAI	GTCTC	4 cut(s)	493, 716, 1844, 2913
BstMCI	CGRYCG	1 cut(s)	1236
BstNI	CCWGG	3 cut(s)	1560, 2175, 3045
BstNSI	RCATGY	5 cut(s)	249, 337, 852, 3364, 3411
BstPI	GGTNACC	1 cut(s)	2648
BstSCI	CCNGG	9 cut(s)	258, 593, 1051, 1138, 1139, 1558, 1905, 2173, 3043
BstSFI	CTRYAG	5 cut(s)	60, 785, 1079, 1432, 2036
BstUI	CGCG	2 cut(s)	1896, 2812
BstV2I	GAAGAC	1 cut(s)	1235
BstX2I	RGATCY	3 cut(s)	535, 796, 1626
BstXI	CCANNNNNNTGG	2 cut(s)	231, 1403
BstYI	RGATCY	3 cut(s)	535, 796, 1626
Bsu15I	ATCGAT	2 cut(s)	128, 1236
Bsu36I	CCTNAGG	1 cut(s)	456
BsuI	GTATCC	1 cut(s)	819
BsuTUI	ATCGAT	2 cut(s)	128, 1236
BtgI	CCRYGG	1 cut(s)	2794
BtsCI	GGATG	8 cut(s)	1119, 2046, 2254, 2417, 2829, 2898, 3166, 3194
BtsI	GCAGTG	1 cut(s)	3084
BtsIMutI	CAGTG	7 cut(s)	376, 1038, 1480, 1664, 1859, 2956, 3084
BveI	ACCTGC	1 cut(s)	3358
Cac8I	GCNNGC	7 cut(s)	850, 861, 1069, 1994, 2411, 2415, 3037
CfoI	GCGC	2 cut(s)	3002, 3428
Cfr10I	RCCGGY	2 cut(s)	518, 818
Cfr13I	GGNCC	8 cut(s)	181, 521, 896, 968, 1143, 1656, 1783, 1826
Cfr9I	CCCGGG	1 cut(s)	1139
ClaI	ATCGAT	2 cut(s)	128, 1236
CseI	GACGC	3 cut(s)	149, 1105, 2818
Csp6I	GTAC	7 cut(s)	65, 300, 1318, 1703, 1819, 2142, 2770
CspAI	ACCGGT	1 cut(s)	818
CspCI	CAANNNNNGTGG	4 cut(s)	1332, 1367, 2876, 2911
CviQI	GTAC	7 cut(s)	65, 300, 1318, 1703, 1819, 2142, 2770
DraIII	CACNNNGTG	1 cut(s)	1670
DrdI	GACNNNNNNGTC	2 cut(s)	116, 2040
DriI	GACNNNNNGTC	1 cut(s)	2926
DseDI	GACNNNNNNGTC	2 cut(s)	116, 2040
EaeI	YGGCCR	3 cut(s)	1990, 2664, 3375
Eam1104I	CTCTTC	2 cut(s)	156, 2733
Eam1105I	GACNNNNNGTC	1 cut(s)	2926
EarI	CTCTTC	2 cut(s)	156, 2733
EciI	GGCGGA	1 cut(s)	2836
Ecl136II	GAGCTC	1 cut(s)	1098
Eco130I	CCWWGG	6 cut(s)	318, 693, 1487, 2644, 2794, 3090
Eco147I	AGGCCT	1 cut(s)	3355
Eco24I	GRGCYC	2 cut(s)	1058, 1100
Eco32I	GATATC	2 cut(s)	1452, 3396
Eco47I	GGWCC	2 cut(s)	181, 1783
Eco47III	AGCGCT	2 cut(s)	3001, 3427
Eco53kI	GAGCTC	1 cut(s)	1098
Eco57I	CTGAAG	1 cut(s)	1247
Eco81I	CCTNAGG	1 cut(s)	456
Eco88I	CYCGRG	2 cut(s)	1139, 2125
Eco91I	GGTNACC	1 cut(s)	2648
EcoICRI	GAGCTC	1 cut(s)	1098
EcoNI	CCTNNNNNAGG	1 cut(s)	837
EcoO109I	RGGNCCY	4 cut(s)	181, 968, 1143, 1783
EcoO65I	GGTNACC	1 cut(s)	2648
EcoRI	GAATTC	3 cut(s)	1674, 2657, 2759
EcoRII	CCWGG	3 cut(s)	1558, 2173, 3043
EcoRV	GATATC	2 cut(s)	1452, 3396
EcoT14I	CCWWGG	6 cut(s)	318, 693, 1487, 2644, 2794, 3090
EcoT22I	ATGCAT	2 cut(s)	850, 2023
EcoT38I	GRGCYC	2 cut(s)	1058, 1100
ErhI	CCWWGG	6 cut(s)	318, 693, 1487, 2644, 2794, 3090
FalI	AAGNNNNNCTT	2 cut(s)	3231, 3263
FaqI	GGGAC	5 cut(s)	1122, 1469, 1934, 2174, 3185
FauI	CCCGC	2 cut(s)	1747, 1889
FauNDI	CATATG	1 cut(s)	403
FblI	GTMKAC	2 cut(s)	784, 3102
FokI	GGATG	8 cut(s)	1106, 2033, 2261, 2404, 2836, 2885, 3173, 3201
FriOI	GRGCYC	2 cut(s)	1058, 1100
FspBI	CTAG	2 cut(s)	329, 2750
GlaI	GCGC	2 cut(s)	3001, 3427
GsuI	CTGGAG	3 cut(s)	953, 1310, 1883
HaeII	RGCGCY	2 cut(s)	3003, 3429
HgaI	GACGC	3 cut(s)	149, 1105, 2818
HhaI	GCGC	2 cut(s)	3002, 3428
Hin1I	GRCGYC	2 cut(s)	1116, 3099
Hin6I	GCGC	2 cut(s)	3000, 3426
HinP1I	GCGC	2 cut(s)	3000, 3426
HincII	GTYRAC	1 cut(s)	3334
HindII	GTYRAC	1 cut(s)	3334
HindIII	AAGCTT	1 cut(s)	872
Hpy166II	GTNNAC	4 cut(s)	376, 785, 3103, 3334
Hpy8I	GTNNAC	4 cut(s)	376, 785, 3103, 3334
Hpy99I	CGWCG	1 cut(s)	143
HpyAV	CCTTC	7 cut(s)	366, 645, 2246, 2262, 2380, 3196, 3206
HpyCH4III	ACNGT	5 cut(s)	668, 2490, 2654, 2877, 2951
HpyCH4IV	ACGT	2 cut(s)	741, 3099
HpySE526I	ACGT	2 cut(s)	741, 3099
Hsp92I	GRCGYC	2 cut(s)	1116, 3099
HspAI	GCGC	2 cut(s)	3000, 3426
LmnI	GCTCC	6 cut(s)	254, 1061, 1074, 1159, 2796, 3127
LweI	GCATC	7 cut(s)	1991, 2008, 2030, 2388, 2426, 2814, 2907
MaeI	CTAG	2 cut(s)	329, 2750
MaeII	ACGT	2 cut(s)	741, 3099
MfeI	CAATTG	2 cut(s)	2355, 2893
MflI	RGATCY	3 cut(s)	535, 796, 1626
MhlI	GDGCHC	3 cut(s)	1058, 1100, 3123
MlsI	TGGCCA	2 cut(s)	1992, 3377
MluI	ACGCGT	1 cut(s)	2810
MluNI	TGGCCA	2 cut(s)	1992, 3377
MlyI	GAGTC	3 cut(s)	1846, 2172, 2196
MmeI	TCCRAC	9 cut(s)	185, 576, 803, 975, 1575, 1786, 2121, 2925, 3166
Mox20I	TGGCCA	2 cut(s)	1992, 3377
Mph1103I	ATGCAT	2 cut(s)	850, 2023
MroXI	GAANNNNTTC	1 cut(s)	1773
MscI	TGGCCA	2 cut(s)	1992, 3377
MseI	TTAA	9 cut(s)	174, 347, 507, 515, 882, 962, 2108, 2403, 2466
MslI	CAYNNNNRTG	5 cut(s)	686, 1401, 2303, 3018, 3325
Msp20I	TGGCCA	2 cut(s)	1992, 3377
MspA1I	CMGCKG	7 cut(s)	107, 231, 1225, 1274, 1431, 2137, 3081
MspR9I	CCNGG	9 cut(s)	260, 595, 1053, 1140, 1141, 1560, 1907, 2175, 3045
MunI	CAATTG	2 cut(s)	2355, 2893
Mva1269I	GAATGC	2 cut(s)	547, 1173
MvaI	CCWGG	3 cut(s)	1560, 2175, 3045
MvnI	CGCG	2 cut(s)	1896, 2812
NciI	CCSGG	6 cut(s)	260, 595, 1053, 1140, 1141, 1907
NcoI	CCATGG	1 cut(s)	2794
NdeI	CATATG	1 cut(s)	403
NlaIV	GGNNCC	9 cut(s)	183, 217, 679, 970, 1057, 1076, 1144, 1657, 1785
NmeAIII	GCCGAG	3 cut(s)	192, 2519, 2692
NmuCI	GTSAC	8 cut(s)	118, 742, 2042, 2648, 2813, 3095, 3133, 3316
NsiI	ATGCAT	2 cut(s)	850, 2023
NspI	RCATGY	5 cut(s)	249, 337, 852, 3364, 3411
OliI	CACNNNNGTG	1 cut(s)	3018
PaeI	GCATGC	1 cut(s)	852
PaeR7I	CTCGAG	1 cut(s)	2125
PaqCI	CACCTGC	1 cut(s)	3358
PceI	AGGCCT	1 cut(s)	3355
PciI	ACATGT	1 cut(s)	3360
PcsI	WCGNNNNNNNCGW	2 cut(s)	747, 2778
PctI	GAATGC	2 cut(s)	547, 1173
PdmI	GAANNNNTTC	1 cut(s)	1773
PfeI	GAWTC	7 cut(s)	496, 980, 1366, 1758, 1975, 2054, 2701
PflMI	CCANNNNNTGG	1 cut(s)	1861
PfoI	TCCNGGA	1 cut(s)	1905
PinAI	ACCGGT	1 cut(s)	818
Ple19I	CGATCG	1 cut(s)	1236
PleI	GAGTC	3 cut(s)	1845, 2172, 2195
PpsI	GAGTC	3 cut(s)	1845, 2172, 2195
PpuMI	RGGWCCY	2 cut(s)	181, 1783
PscI	ACATGT	1 cut(s)	3360
PshBI	ATTAAT	1 cut(s)	2466
Psp124BI	GAGCTC	1 cut(s)	1100
Psp5II	RGGWCCY	2 cut(s)	181, 1783
Psp6I	CCWGG	3 cut(s)	1558, 2173, 3043
PspEI	GGTNACC	1 cut(s)	2648
PspGI	CCWGG	3 cut(s)	1558, 2173, 3043
PspN4I	GGNNCC	9 cut(s)	183, 217, 679, 970, 1057, 1076, 1144, 1657, 1785
PspPI	GGNCC	8 cut(s)	181, 521, 896, 968, 1143, 1656, 1783, 1826
PspPPI	RGGWCCY	2 cut(s)	181, 1783
PspXI	VCTCGAGB	1 cut(s)	2125
PsrI	GAACNNNNNNTAC	2 cut(s)	661, 693
PstI	CTGCAG	3 cut(s)	64, 1436, 2040
PsuI	RGATCY	3 cut(s)	535, 796, 1626
PvuI	CGATCG	1 cut(s)	1236
PvuII	CAGCTG	6 cut(s)	107, 231, 1225, 1274, 1431, 2137
RsaI	GTAC	7 cut(s)	66, 301, 1319, 1704, 1820, 2143, 2771
RsaNI	GTAC	7 cut(s)	65, 300, 1318, 1703, 1819, 2142, 2770
RseI	CAYNNNNRTG	5 cut(s)	686, 1401, 2303, 3018, 3325
SacI	GAGCTC	1 cut(s)	1100
SaqAI	TTAA	9 cut(s)	174, 347, 507, 515, 882, 962, 2108, 2403, 2466
Sau96I	GGNCC	8 cut(s)	181, 521, 896, 968, 1143, 1656, 1783, 1826
ScaI	AGTACT	1 cut(s)	66
SchI	GAGTC	3 cut(s)	1846, 2172, 2196
ScrFI	CCNGG	9 cut(s)	260, 595, 1053, 1140, 1141, 1560, 1907, 2175, 3045
SduI	GDGCHC	3 cut(s)	1058, 1100, 3123
SfaNI	GCATC	7 cut(s)	1991, 2008, 2030, 2388, 2426, 2814, 2907
SfcI	CTRYAG	5 cut(s)	60, 785, 1079, 1432, 2036
Sfr274I	CTCGAG	1 cut(s)	2125
SinI	GGWCC	2 cut(s)	181, 1783
SlaI	CTCGAG	1 cut(s)	2125
SmaI	CCCGGG	1 cut(s)	1141
SmiMI	CAYNNNNRTG	5 cut(s)	686, 1401, 2303, 3018, 3325
SphI	GCATGC	1 cut(s)	852
SseBI	AGGCCT	1 cut(s)	3355
SsiI	CCGC	9 cut(s)	778, 852, 1517, 1754, 1896, 2365, 2426, 2821, 3079
SspI	AATATT	1 cut(s)	626
SspMI	CTAG	2 cut(s)	329, 2750
SstI	GAGCTC	1 cut(s)	1100
StuI	AGGCCT	1 cut(s)	3355
StyD4I	CCNGG	9 cut(s)	258, 593, 1051, 1138, 1139, 1558, 1905, 2173, 3043
StyI	CCWWGG	6 cut(s)	318, 693, 1487, 2644, 2794, 3090
TaaI	ACNGT	5 cut(s)	668, 2490, 2654, 2877, 2951
TaiI	ACGT	2 cut(s)	744, 3102
TatI	WGTACW	2 cut(s)	64, 1818
TauI	GCSGC	1 cut(s)	2429
TfiI	GAWTC	7 cut(s)	496, 980, 1366, 1758, 1975, 2054, 2701
Tru1I	TTAA	9 cut(s)	174, 347, 507, 515, 882, 962, 2108, 2403, 2466
Tru9I	TTAA	9 cut(s)	174, 347, 507, 515, 882, 962, 2108, 2403, 2466
TscAI	CASTG	7 cut(s)	376, 1045, 1480, 1671, 1866, 2956, 3091
TseFI	GTSAC	8 cut(s)	118, 742, 2042, 2648, 2813, 3095, 3133, 3316
Tsp45I	GTSAC	8 cut(s)	118, 742, 2042, 2648, 2813, 3095, 3133, 3316
TspGWI	ACGGA	2 cut(s)	1720, 2787
TspMI	CCCGGG	1 cut(s)	1139
TspRI	CASTG	7 cut(s)	376, 1045, 1480, 1671, 1866, 2956, 3091
Van91I	CCANNNNNTGG	1 cut(s)	1861
VpaK11BI	GGWCC	2 cut(s)	181, 1783
VspI	ATTAAT	1 cut(s)	2466
XagI	CCTNNNNNAGG	1 cut(s)	837
XapI	RAATTY	8 cut(s)	365, 1194, 1294, 1493, 1674, 2118, 2657, 2759
XbaI	TCTAGA	1 cut(s)	328
XceI	RCATGY	5 cut(s)	249, 337, 852, 3364, 3411
XhoI	CTCGAG	1 cut(s)	2125
XmaI	CCCGGG	1 cut(s)	1139
XmiI	GTMKAC	2 cut(s)	784, 3102
XmnI	GAANNNNTTC	1 cut(s)	1773
XspI	CTAG	2 cut(s)	329, 2750
ZraI	GACGTC	1 cut(s)	3100
ZrmI	AGTACT	1 cut(s)	66
Zsp2I	ATGCAT	2 cut(s)	850, 2023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.