Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011739

Physical Location & Seq

Genomic Coordinates Reverse (-)

3516168 .. 3519980

Gene Structure 3813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1049700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGCCATCGCAGCAGATTCTGCCCATAACATATCACCCCCCTTCAGAGAAATCGCTACGCAAAGAAGCAGAGGACGACCCGACAACCGGGCTTCTCTTGTACTCGAATTTCGAGGATGAAGAAAAGAACACGCCTCTCAAGCCCCTTCTCCCCAGGGTCTCCAGCTACACAACCACCAGCGCTGCCGCTGCCAACAACCACCAGCAACGGCGGCGCCGGACCGCCAGCGACAGCTCCCTTTCTTCCCACTCCGATGAGGGTAGCCGCCACTCCTTGGCCGGCGATGTGGAGCACGCTGCTGCCGAGACGTTCTTGGTGACCCGGTTGAGTTTGAAGCTGTTAAAATATCTTGGGGTAGGCTACAGATGGATTACAAGATTTCTCGCCCTTGGTTGCTATTCGTTTTTACTTATGCCAGGTTTTATTCAAGTTGGCTATTATTATTTCTTCTCCCGTCAGGTTCGCAGAGGTATAGTTTATGGTGATCAACCAAGAAATAGGCTAGATCTGTATCTACCCAAAAATAGCGATGGGCCAAAGCCAGTCATTGCATTTGTAACTGGTGGAGCCTGGATTATTGGTGTGGCTGAGAGTTACAAAGCATGGGGTTCTCTTTTAGGACAGCAGTTATCAGAAAGAGACATCATAGTGGCATGCATAGATTACAGAAATTTTCCTCAGGGTACTATCAGTGATATGGTAAAGGATGCTTCTCAAGGCATCTCATTTGTGTGCAATCATATTGCTGATTATGGAGGCGACCCAAATAGGGTTTATGTAATGGGACAATCGGCTGGTGCACATATTGGTGCCTGCACCCTCGTTGACCAGGCAATTAAGGAGGCCGGCGAAGGAGAGAGCTCTTCTTGGAGTCTCTCCCAGATAAAGGCTTACTTTGGTTTGTCTGGAGGGTACAATTTGCTTAACTTGGTGGATCACTTCCACCGCCGAGGTCTGTATCGTTCAATCTTTTTAGGCATAATGGAAGGAGAACAATCTTTGCAACGTTTTTCTCCAGAACTCATGATTCAGGACCAGAATGTTAGAAATGCTGCTTCTCTCCTACCTCCTATTATTTTGTTTCATGGTACTGCAGATTACTCCATACCATCAGATGCCAGCAAGAATTTTGCAGAAACTCTTCAGAGACTTGGAGTGACAGCTGAGTCGATTCTTTATGAAGGGAAGACTCATACGGATTTGTTTCTTCAGGATCCAATGCGTGGTGGTAGAGATGATATGTTTGAAGATTTAGTAGCTATCATTCATGAAGGTGATTTAGAGGCTCTCGCCAAAGATGCTGTGGCTCCGCCAAGAAGGCGCCTTGTGCCTGAGTGCATGTTGAAGTTGGCTCACCGTGTCAGCCCGTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0008150 GO:0008152 GO:0009056 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010296 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0031090 GO:0031984 GO:0033993 GO:0042175 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051723 GO:0052689 GO:0097305 GO:0098588 GO:0098791 GO:0098827 GO:0140096 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00900 ko01120 ko01130 map00900 map01120 map01130

Pfam Domains

Protein Families

Abhydrolase_3 COesterase

Protein Analysis

458

Amino Acids

50.78

Weight (kDa)

6.24

Isoelectric Point (pI)

46.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BD-FAE	PF20434	168 - 381	3.6e-29	BD-FAE
COesterase	PF00135	168 - 269	3.1e-12	Carboxylesterase family
Abhydrolase_3	PF07859	185 - 398	1.4e-15	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G26120
fragaria_vesca	FvH4_4g28400 FvH4_4g28400 FvH4_4g28400
malus_domestica	MD13G1049700.v1.1 MD16G1050700.v1.1
prunus_persica	Prupe.1G302000_v2.0.a1
pyrus_communis	pycom13g04400 pycom16g04440
rosa_chinensis	RchiOBHm_Chr4g0436331
rosa_laevigata	RLG00000006526
rosa_multiflora	Rmu_sc0007205.1_g000008
rosa_roxburghii	Rroxscaffold_5G00377510
rosa_rugosa	Rorug04G0293200
rosa_samantha	Rh4AG347300 Rh4BG356200 Rh4CG370800 Rh4DG349900
rosa_wichuraiana	Rw4G030360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1165
AccB1I	GGYRCC	3 cut(s)	213, 809, 1320
AccB7I	CCANNNNNTGG	2 cut(s)	274, 1223
AciI	CCGC	6 cut(s)	186, 211, 222, 265, 946, 1310
AclI	AACGTT	1 cut(s)	1006
AclWI	GGATC	3 cut(s)	942, 1208, 1221
AcoI	YGGCCR	1 cut(s)	276
AcsI	RAATTY	3 cut(s)	106, 670, 1126
AcuI	CTGAAG	3 cut(s)	27, 1127, 1193
AcyI	GRCGYC	2 cut(s)	214, 1321
AfaI	GTAC	4 cut(s)	101, 685, 914, 1090
AfeI	AGCGCT	1 cut(s)	181
AfiI	CCNNNNNNNGG	5 cut(s)	86, 274, 769, 886, 1223
AgsI	TTSAA	5 cut(s)	334, 428, 966, 1247, 1345
AjnI	CCWGG	4 cut(s)	152, 415, 569, 828
AluBI	AGCT	6 cut(s)	165, 234, 337, 861, 1163, 1259
AluI	AGCT	6 cut(s)	165, 234, 337, 861, 1163, 1259
Alw21I	GWGCWC	3 cut(s)	294, 802, 863
Alw26I	GTCTC	5 cut(s)	163, 299, 633, 878, 1141
Alw44I	GTGCAC	1 cut(s)	798
AlwI	GGATC	3 cut(s)	942, 1208, 1221
AlwNI	CAGNNNCTG	1 cut(s)	19
Aor51HI	AGCGCT	1 cut(s)	181
AoxI	GGCC	3 cut(s)	276, 533, 843
ApaLI	GTGCAC	1 cut(s)	798
ApeKI	GCWGC	6 cut(s)	10, 182, 188, 296, 299, 1052
ApoI	RAATTY	3 cut(s)	106, 670, 1126
Asp700I	GAANNNNTTC	1 cut(s)	1140
AspLEI	GCGC	3 cut(s)	182, 216, 1323
AspS9I	GGNCC	3 cut(s)	219, 533, 1033
AsuC2I	CCSGG	2 cut(s)	88, 322
AsuHPI	GGTGA	5 cut(s)	26, 328, 494, 1286, 1346
AvaII	GGWCC	2 cut(s)	219, 1033
AxyI	CCTNAGG	1 cut(s)	678
BaeGI	GKGCMC	1 cut(s)	802
BamHI	GGATCC	1 cut(s)	1213
BanI	GGYRCC	3 cut(s)	213, 809, 1320
BanII	GRGCYC	1 cut(s)	863
BbsI	GAAGAC	1 cut(s)	1193
Bbv12I	GWGCWC	3 cut(s)	294, 802, 863
BbvI	GCAGC	6 cut(s)	22, 169, 175, 283, 286, 1039
BccI	CCATC	4 cut(s)	13, 360, 524, 1117
BceAI	ACGGC	1 cut(s)	224
BciT130I	CCWGG	4 cut(s)	154, 417, 571, 830
BclI	TGATCA	1 cut(s)	484
BcnI	CCSGG	2 cut(s)	88, 322
BcoDI	GTCTC	5 cut(s)	163, 299, 633, 878, 1141
BfaI	CTAG	2 cut(s)	503, 1372
BfmI	CTRYAG	2 cut(s)	361, 1092
BfoI	RGCGCY	3 cut(s)	183, 217, 1324
BglI	GCCNNNNNGGC	1 cut(s)	1318
BglII	AGATCT	1 cut(s)	505
BisI	GCNGC	9 cut(s)	11, 183, 186, 189, 212, 265, 297, 300, 1053
BlsI	GCNGC	9 cut(s)	12, 184, 187, 190, 213, 266, 298, 301, 1054
Bme1390I	CCNGG	6 cut(s)	88, 154, 322, 417, 571, 830
Bme18I	GGWCC	2 cut(s)	219, 1033
BmgT120I	GGNCC	3 cut(s)	219, 533, 1033
BmiI	GGNNCC	6 cut(s)	215, 568, 811, 1215, 1308, 1322
BmrFI	CCNGG	6 cut(s)	88, 154, 322, 417, 571, 830
BmsI	GCATC	4 cut(s)	697, 729, 1105, 1288
BpiI	GAAGAC	1 cut(s)	1193
BpmI	CTGGAG	3 cut(s)	145, 927, 999
BpuEI	CTTGAG	2 cut(s)	122, 699
BpuMI	CCSGG	2 cut(s)	88, 322
BsaBI	GATNNNNATC	1 cut(s)	510
BsaHI	GRCGYC	2 cut(s)	214, 1321
BsaI	GGTCTC	1 cut(s)	163
BsaJI	CCNNGG	5 cut(s)	152, 153, 273, 388, 949
Bsc4I	CCNNNNNNNGG	5 cut(s)	86, 274, 769, 886, 1223
Bse118I	RCCGGY	2 cut(s)	278, 845
Bse1I	ACTGG	2 cut(s)	542, 565
Bse21I	CCTNAGG	1 cut(s)	678
Bse3DI	GCAATG	1 cut(s)	546
Bse8I	GATNNNNATC	1 cut(s)	510
BseBI	CCWGG	4 cut(s)	154, 417, 571, 830
BseDI	CCNNGG	5 cut(s)	152, 153, 273, 388, 949
BseGI	GGATG	2 cut(s)	121, 712
BseJI	GATNNNNATC	1 cut(s)	510
BseLI	CCNNNNNNNGG	5 cut(s)	86, 274, 769, 886, 1223
BseMI	GCAATG	1 cut(s)	546
BseMII	CTCAG	4 cut(s)	579, 692, 1155, 1323
BseNI	ACTGG	2 cut(s)	542, 565
BseSI	GKGCMC	1 cut(s)	802
BseXI	GCAGC	6 cut(s)	22, 169, 175, 283, 286, 1039
BsgI	GTGCAG	1 cut(s)	799
BshFI	GGCC	3 cut(s)	278, 535, 845
BshNI	GGYRCC	3 cut(s)	213, 809, 1320
BsiHKAI	GWGCWC	3 cut(s)	294, 802, 863
BsiSI	CCGG	5 cut(s)	87, 217, 279, 322, 846
BslFI	GGGAC	1 cut(s)	798
BslI	CCNNNNNNNGG	5 cut(s)	86, 274, 769, 886, 1223
BsmAI	GTCTC	5 cut(s)	163, 299, 633, 878, 1141
BsmBI	CGTCTC	1 cut(s)	299
BsmFI	GGGAC	1 cut(s)	798
BsnI	GGCC	3 cut(s)	278, 535, 845
Bso31I	GGTCTC	1 cut(s)	163
Bsp1286I	GDGCHC	3 cut(s)	294, 802, 863
Bsp143I	GATC	4 cut(s)	484, 505, 934, 1213
BspACI	CCGC	6 cut(s)	186, 211, 222, 265, 946, 1310
BspANI	GGCC	3 cut(s)	278, 535, 845
BspCNI	CTCAG	4 cut(s)	580, 691, 1156, 1324
BspHI	TCATGA	2 cut(s)	1023, 1267
BspLI	GGNNCC	6 cut(s)	215, 568, 811, 1215, 1308, 1322
BspMAI	CTGCAG	1 cut(s)	1096
BspPI	GGATC	3 cut(s)	942, 1208, 1221
BspQI	GCTCTTC	1 cut(s)	868
BspT107I	GGYRCC	3 cut(s)	213, 809, 1320
BspTNI	GGTCTC	1 cut(s)	163
BsrDI	GCAATG	1 cut(s)	546
BsrFI	RCCGGY	2 cut(s)	278, 845
BsrI	ACTGG	2 cut(s)	542, 565
BssAI	RCCGGY	2 cut(s)	278, 845
BssECI	CCNNGG	5 cut(s)	152, 153, 273, 388, 949
BssMI	GATC	4 cut(s)	484, 505, 934, 1213
BssNI	GRCGYC	2 cut(s)	214, 1321
BssT1I	CCWWGG	2 cut(s)	273, 388
Bst2UI	CCWGG	4 cut(s)	154, 417, 571, 830
Bst4CI	ACNGT	1 cut(s)	1358
Bst6I	CTCTTC	2 cut(s)	868, 1146
BstACI	GRCGYC	2 cut(s)	214, 1321
BstAPI	GCANNNNNTGC	1 cut(s)	19
BstC8I	GCNNGC	7 cut(s)	226, 280, 294, 655, 814, 847, 1120
BstDEI	CTNAG	4 cut(s)	588, 678, 1164, 1332
BstEII	GGTNACC	1 cut(s)	316
BstF5I	GGATG	2 cut(s)	121, 712
BstH2I	RGCGCY	3 cut(s)	183, 217, 1324
BstHHI	GCGC	3 cut(s)	182, 216, 1323
BstKTI	GATC	4 cut(s)	487, 508, 937, 1216
BstMAI	GTCTC	5 cut(s)	163, 299, 633, 878, 1141
BstMBI	GATC	4 cut(s)	484, 505, 934, 1213
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 19, 139, 188, 211, 1298, 1318, 1327
BstNI	CCWGG	4 cut(s)	154, 417, 571, 830
BstNSI	RCATGY	2 cut(s)	657, 1342
BstPI	GGTNACC	1 cut(s)	316
BstSCI	CCNGG	6 cut(s)	86, 152, 320, 415, 569, 828
BstSFI	CTRYAG	2 cut(s)	361, 1092
BstSLI	GKGCMC	1 cut(s)	802
BstV1I	GCAGC	6 cut(s)	22, 169, 175, 283, 286, 1039
BstV2I	GAAGAC	1 cut(s)	1193
BstX2I	RGATCY	2 cut(s)	505, 1213
BstYI	RGATCY	2 cut(s)	505, 1213
Bsu36I	CCTNAGG	1 cut(s)	678
BsuRI	GGCC	3 cut(s)	278, 535, 845
BtgZI	GCGATG	2 cut(s)	297, 543
BtsCI	GGATG	2 cut(s)	121, 712
BtsIMutI	CAGTG	1 cut(s)	697
Cac8I	GCNNGC	7 cut(s)	226, 280, 294, 655, 814, 847, 1120
CaiI	CAGNNNCTG	1 cut(s)	19
CciI	TCATGA	2 cut(s)	1023, 1267
CfoI	GCGC	3 cut(s)	182, 216, 1323
Cfr10I	RCCGGY	2 cut(s)	278, 845
Cfr13I	GGNCC	3 cut(s)	219, 533, 1033
CpoI	CGGWCCG	1 cut(s)	219
Csp6I	GTAC	4 cut(s)	100, 684, 913, 1089
CspI	CGGWCCG	1 cut(s)	219
CviAII	CATG	6 cut(s)	603, 654, 1024, 1085, 1268, 1339
CviQI	GTAC	4 cut(s)	100, 684, 913, 1089
DdeI	CTNAG	4 cut(s)	588, 678, 1164, 1332
DinI	GGCGCC	2 cut(s)	215, 1322
DpnI	GATC	4 cut(s)	486, 507, 936, 1215
DpnII	GATC	4 cut(s)	484, 505, 934, 1213
DrdI	GACNNNNNNGTC	1 cut(s)	1165
DseDI	GACNNNNNNGTC	1 cut(s)	1165
EaeI	YGGCCR	1 cut(s)	276
Eam1104I	CTCTTC	2 cut(s)	868, 1146
EarI	CTCTTC	2 cut(s)	868, 1146
EciI	GGCGGA	1 cut(s)	1299
Ecl136II	GAGCTC	1 cut(s)	861
Eco130I	CCWWGG	2 cut(s)	273, 388
Eco24I	GRGCYC	1 cut(s)	863
Eco31I	GGTCTC	1 cut(s)	163
Eco47I	GGWCC	2 cut(s)	219, 1033
Eco47III	AGCGCT	1 cut(s)	181
Eco53kI	GAGCTC	1 cut(s)	861
Eco57I	CTGAAG	3 cut(s)	27, 1127, 1193
Eco81I	CCTNAGG	1 cut(s)	678
Eco91I	GGTNACC	1 cut(s)	316
EcoICRI	GAGCTC	1 cut(s)	861
EcoO65I	GGTNACC	1 cut(s)	316
EcoRII	CCWGG	4 cut(s)	152, 415, 569, 828
EcoT14I	CCWWGG	2 cut(s)	273, 388
EcoT22I	ATGCAT	1 cut(s)	659
EcoT38I	GRGCYC	1 cut(s)	863
EgeI	GGCGCC	2 cut(s)	215, 1322
EheI	GGCGCC	2 cut(s)	215, 1322
ErhI	CCWWGG	2 cut(s)	273, 388
Esp3I	CGTCTC	1 cut(s)	299
FaeI	CATG	6 cut(s)	606, 657, 1027, 1088, 1271, 1342
FalI	AAGNNNNNCTT	2 cut(s)	878, 910
FaqI	GGGAC	1 cut(s)	798
FatI	CATG	6 cut(s)	602, 653, 1023, 1084, 1267, 1338
FbaI	TGATCA	1 cut(s)	484
Fnu4HI	GCNGC	9 cut(s)	11, 183, 186, 189, 212, 265, 297, 300, 1053
FokI	GGATG	2 cut(s)	128, 719
FriOI	GRGCYC	1 cut(s)	863
Fsp4HI	GCNGC	9 cut(s)	11, 183, 186, 189, 212, 265, 297, 300, 1053
FspBI	CTAG	2 cut(s)	503, 1372
GlaI	GCGC	3 cut(s)	181, 215, 1322
GluI	GCNGC	9 cut(s)	11, 183, 186, 189, 212, 265, 297, 300, 1053
GsuI	CTGGAG	3 cut(s)	145, 927, 999
HaeII	RGCGCY	3 cut(s)	183, 217, 1324
HaeIII	GGCC	3 cut(s)	278, 535, 845
HapII	CCGG	5 cut(s)	87, 217, 279, 322, 846
HhaI	GCGC	3 cut(s)	182, 216, 1323
Hin1I	GRCGYC	2 cut(s)	214, 1321
Hin1II	CATG	6 cut(s)	606, 657, 1027, 1088, 1271, 1342
Hin6I	GCGC	3 cut(s)	180, 214, 1321
HinP1I	GCGC	3 cut(s)	180, 214, 1321
HincII	GTYRAC	1 cut(s)	826
HindII	GTYRAC	1 cut(s)	826
HinfI	GANTC	6 cut(s)	16, 871, 1027, 1166, 1171, 1189
HpaII	CCGG	5 cut(s)	87, 217, 279, 322, 846
HphI	GGTGA	5 cut(s)	26, 328, 494, 1286, 1346
Hpy166II	GTNNAC	2 cut(s)	800, 826
Hpy188I	TCNGA	5 cut(s)	46, 253, 634, 1114, 1146
Hpy188III	TCNNGA	6 cut(s)	906, 1016, 1024, 1031, 1211, 1268
Hpy8I	GTNNAC	2 cut(s)	800, 826
HpyAV	CCTTC	7 cut(s)	51, 155, 845, 980, 1175, 1265, 1311
HpyCH4III	ACNGT	1 cut(s)	1358
HpyCH4IV	ACGT	2 cut(s)	308, 1006
HpyCH4V	TGCA	9 cut(s)	551, 657, 735, 800, 816, 1003, 1094, 1133, 1338
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 19, 139, 188, 211, 1298, 1318, 1327
HpyF3I	CTNAG	4 cut(s)	588, 678, 1164, 1332
HpySE526I	ACGT	2 cut(s)	308, 1006
Hsp92I	GRCGYC	2 cut(s)	214, 1321
Hsp92II	CATG	6 cut(s)	606, 657, 1027, 1088, 1271, 1342
HspAI	GCGC	3 cut(s)	180, 214, 1321
KasI	GGCGCC	2 cut(s)	213, 1320
KroI	GCCGGC	2 cut(s)	278, 845
KroNI	GCCGGC	2 cut(s)	280, 847
Ksp22I	TGATCA	1 cut(s)	484
Kzo9I	GATC	4 cut(s)	484, 505, 934, 1213
LguI	GCTCTTC	1 cut(s)	868
LmnI	GCTCC	4 cut(s)	239, 289, 566, 1312
Lsp1109I	GCAGC	6 cut(s)	22, 169, 175, 283, 286, 1039
LweI	GCATC	4 cut(s)	697, 729, 1105, 1288
MaeI	CTAG	2 cut(s)	503, 1372
MaeII	ACGT	2 cut(s)	308, 1006
MaeIII	GTNAC	4 cut(s)	316, 556, 593, 1156
MalI	GATC	4 cut(s)	486, 507, 936, 1215
MboI	GATC	4 cut(s)	484, 505, 934, 1213
MboII	GAAGA	8 cut(s)	131, 234, 439, 855, 1133, 1198, 1199, 1259
MflI	RGATCY	2 cut(s)	505, 1213
MhlI	GDGCHC	3 cut(s)	294, 802, 863
MluCI	AATT	5 cut(s)	106, 670, 834, 916, 1126
Mly113I	GGCGCC	2 cut(s)	214, 1321
MlyI	GAGTC	3 cut(s)	880, 1175, 1183
Mph1103I	ATGCAT	1 cut(s)	659
MroNI	GCCGGC	2 cut(s)	278, 845
MroXI	GAANNNNTTC	1 cut(s)	1140
MseI	TTAA	3 cut(s)	341, 837, 924
MslI	CAYNNNNRTG	5 cut(s)	252, 647, 730, 807, 1272
MspA1I	CMGCKG	2 cut(s)	188, 1163
MspI	CCGG	5 cut(s)	87, 217, 279, 322, 846
MspR9I	CCNGG	6 cut(s)	88, 154, 322, 417, 571, 830
MvaI	CCWGG	4 cut(s)	154, 417, 571, 830
MwoI	GCNNNNNNNGC	8 cut(s)	10, 19, 139, 188, 211, 1298, 1318, 1327
NaeI	GCCGGC	2 cut(s)	280, 847
NarI	GGCGCC	2 cut(s)	214, 1321
NciI	CCSGG	2 cut(s)	88, 322
NdeII	GATC	4 cut(s)	484, 505, 934, 1213
NgoMIV	GCCGGC	2 cut(s)	278, 845
NlaIII	CATG	6 cut(s)	606, 657, 1027, 1088, 1271, 1342
NlaIV	GGNNCC	6 cut(s)	215, 568, 811, 1215, 1308, 1322
NmeAIII	GCCGAG	2 cut(s)	328, 974
NmuCI	GTSAC	2 cut(s)	316, 1156
NsiI	ATGCAT	1 cut(s)	659
NspI	RCATGY	2 cut(s)	657, 1342
PaeI	GCATGC	1 cut(s)	657
PagI	TCATGA	2 cut(s)	1023, 1267
PasI	CCCWGGG	1 cut(s)	153
PciSI	GCTCTTC	1 cut(s)	868
PcsI	WCGNNNNNNNCGW	1 cut(s)	300
PdiI	GCCGGC	2 cut(s)	280, 847
PdmI	GAANNNNTTC	1 cut(s)	1140
PfeI	GAWTC	3 cut(s)	16, 1027, 1171
PflMI	CCANNNNNTGG	2 cut(s)	274, 1223
PkrI	GCNGC	9 cut(s)	12, 184, 187, 190, 213, 266, 298, 301, 1054
PleI	GAGTC	3 cut(s)	879, 1174, 1183
PluTI	GGCGCC	2 cut(s)	217, 1324
PpsI	GAGTC	3 cut(s)	879, 1174, 1183
Psp124BI	GAGCTC	1 cut(s)	863
Psp1406I	AACGTT	1 cut(s)	1006
Psp6I	CCWGG	4 cut(s)	152, 415, 569, 828
PspEI	GGTNACC	1 cut(s)	316
PspGI	CCWGG	4 cut(s)	152, 415, 569, 828
PspN4I	GGNNCC	6 cut(s)	215, 568, 811, 1215, 1308, 1322
PspPI	GGNCC	3 cut(s)	219, 533, 1033
PstI	CTGCAG	1 cut(s)	1096
PstNI	CAGNNNCTG	1 cut(s)	19
PsuI	RGATCY	2 cut(s)	505, 1213
PvuII	CAGCTG	1 cut(s)	1163
RsaI	GTAC	4 cut(s)	101, 685, 914, 1090
RsaNI	GTAC	4 cut(s)	100, 684, 913, 1089
RseI	CAYNNNNRTG	5 cut(s)	252, 647, 730, 807, 1272
Rsr2I	CGGWCCG	1 cut(s)	219
RsrII	CGGWCCG	1 cut(s)	219
SacI	GAGCTC	1 cut(s)	863
SapI	GCTCTTC	1 cut(s)	868
SaqAI	TTAA	3 cut(s)	341, 837, 924
SatI	GCNGC	9 cut(s)	11, 183, 186, 189, 212, 265, 297, 300, 1053
Sau3AI	GATC	4 cut(s)	484, 505, 934, 1213
Sau96I	GGNCC	3 cut(s)	219, 533, 1033
SchI	GAGTC	3 cut(s)	880, 1175, 1183
ScrFI	CCNGG	6 cut(s)	88, 154, 322, 417, 571, 830
SduI	GDGCHC	3 cut(s)	294, 802, 863
SfaNI	GCATC	4 cut(s)	697, 729, 1105, 1288
SfcI	CTRYAG	2 cut(s)	361, 1092
SfoI	GGCGCC	2 cut(s)	215, 1322
SinI	GGWCC	2 cut(s)	219, 1033
SmiMI	CAYNNNNRTG	5 cut(s)	252, 647, 730, 807, 1272
SmlI	CTYRAG	2 cut(s)	137, 714
SmoI	CTYRAG	2 cut(s)	137, 714
SphI	GCATGC	1 cut(s)	657
Sse9I	AATT	5 cut(s)	106, 670, 834, 916, 1126
SsiI	CCGC	6 cut(s)	186, 211, 222, 265, 946, 1310
SspDI	GGCGCC	2 cut(s)	213, 1320
SspMI	CTAG	2 cut(s)	503, 1372
SstI	GAGCTC	1 cut(s)	863
StyD4I	CCNGG	6 cut(s)	86, 152, 320, 415, 569, 828
StyI	CCWWGG	2 cut(s)	273, 388
TaaI	ACNGT	1 cut(s)	1358
TaiI	ACGT	2 cut(s)	311, 1009
TaqI	TCGA	3 cut(s)	104, 111, 1169
TasI	AATT	5 cut(s)	106, 670, 834, 916, 1126
TatI	WGTACW	1 cut(s)	99
TauI	GCSGC	3 cut(s)	188, 214, 267
TfiI	GAWTC	3 cut(s)	16, 1027, 1171
Tru1I	TTAA	3 cut(s)	341, 837, 924
Tru9I	TTAA	3 cut(s)	341, 837, 924
TscAI	CASTG	1 cut(s)	697
TseFI	GTSAC	2 cut(s)	316, 1156
TseI	GCWGC	6 cut(s)	10, 182, 188, 296, 299, 1052
Tsp45I	GTSAC	2 cut(s)	316, 1156
TspDTI	ATGAA	5 cut(s)	132, 1073, 1194, 1256, 1284
TspGWI	ACGGA	1 cut(s)	1211
TspRI	CASTG	1 cut(s)	697
Van91I	CCANNNNNTGG	2 cut(s)	274, 1223
VneI	GTGCAC	1 cut(s)	798
VpaK11BI	GGWCC	2 cut(s)	219, 1033
XapI	RAATTY	3 cut(s)	106, 670, 1126
XceI	RCATGY	2 cut(s)	657, 1342
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1300
XmnI	GAANNNNTTC	1 cut(s)	1140
XspI	CTAG	2 cut(s)	503, 1372
Zsp2I	ATGCAT	1 cut(s)	659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.