MD13G1062800.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015607
Physical Location & Seq
Reverse (-)
4367180 .. 4369118
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1062800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGCCGCCGTCGGCCTCAGAAACCCTACTTCTCATCGCCATTTTCTTCTTGCTTCCTCTGGACACCCTCTCGTCCGTCATCCACGCAGGCCATGCCATCCCTCTCACTGGTATGTGTGAGGACGCATGCGGTGCAATCCCAGTAAAGTTCCCATTCGGTACCGGATTTGGTTGCGGCCACCCTGAATTTATCAGATACATCAAATGCAGCTCAGGAACACTTCAATTCTCCACCGGAACCGGCATCTACACCATTTCCTCCATAGACTACAACAGCAGCACCATCATTGTCACAGATCCACTCATGTCAACATGTTCCTCCATGCAAAATTCAGGTAGTTTTAGCTTGGACAAGTCCAGTCCTTTCACTATAGGAGAGCAAAACATCTTCGTTTTGCTCGGCTGCTCAACGACATCCCCGGTGTTTGATCCAAACGAAGACTTGTGTGACACTGGATCCGGTACCCGGGTGTGTAGAGGGTTGTACTCTTGCAAGGGGGTTGCCGGCATTGGACTGCCACAGAATGCGCCGATTTCCACCTGTTGTGTTTATGATTCTGAAATCGTGGTTGGCTCAGGTTATGCACTGGATCTTCCAAAGCTCCAGTGCTCTTCTTATACATCAGTTTACGAGTTTGGAGACGAAGGCGATCCGACGAAATGGAGATTTGGGATTTCTTTGCAATATAATGATTCTTACCACACAAATAAATGCAAGGATTGTGAAACCAGTGGAGGGATTTGTGGATTTGCTGGTTTGGATCAGTCATTTGCTTGTATTTGCAGGAGTGGTGTTAACACCACCACAAATTGTTTTGGGCGAGGGTACGCTTGGAGTGGGGCAGTGGATCTGGATTTTCAAGTTAACACCATTATTGGCGGACTTTTGCTCTCGTGGGCCTTGCTATTTATATGTAATCAGCAGATAGAGCTCCAAGACCTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

316

Amino Acids

33.6

Weight (kDa)

4.46

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 99 7e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 224 - 263 6e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015607)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30030 FvH4_4g30030
malus_domestica MD13G1062800.v1.1
prunus_persica Prupe.1G286900_v2.0.a1
pyrus_communis pycom13g05520
rosa_chinensis RchiOBHm_Chr4g0438321
rosa_laevigata RLG00000006370
rosa_multiflora Rmu_sc0011129.1_g000001
rosa_roxburghii Rroxscaffold_5G00379410
rosa_rugosa Rorug04G0309000
rosa_samantha Rh4AG360100 Rh4BG371100 Rh4CG385100 Rh4DG366000
rosa_wichuraiana Rw0G006470 Rw0G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 158, 461
AccB1I GGYRCC 2 cut(s) 158, 461
AciI CCGC 4 cut(s) 5, 129, 174, 879
AclWI GGATC 8 cut(s) 290, 422, 450, 463, 597, 644, 768, 855
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 2 cut(s) 185, 328
AfaI GTAC 4 cut(s) 160, 463, 485, 827
AfiI CCNNNNNNNGG 3 cut(s) 107, 465, 735
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 2 cut(s) 224, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 4 cut(s) 210, 345, 601, 931
AluI AGCT 4 cut(s) 210, 345, 601, 931
Alw21I GWGCWC 2 cut(s) 611, 933
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 8 cut(s) 290, 422, 450, 463, 597, 644, 768, 855
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 4 cut(s) 12, 88, 175, 897
ApeKI GCWGC 3 cut(s) 207, 276, 402
ApoI RAATTY 2 cut(s) 185, 328
Asp718I GGTACC 2 cut(s) 158, 461
AspLEI GCGC 1 cut(s) 529
AspS9I GGNCC 1 cut(s) 897
AsuC2I CCSGG 3 cut(s) 419, 466, 467
AvaI CYCGRG 1 cut(s) 465
BaeI ACNNNNGTAYC 2 cut(s) 453, 486
BamHI GGATCC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 158, 461
BanII GRGCYC 1 cut(s) 933
BauI CACGAG 1 cut(s) 892
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 2 cut(s) 611, 933
BbvI GCAGC 3 cut(s) 219, 288, 389
BccI CCATC 2 cut(s) 104, 290
BcnI CCSGG 3 cut(s) 419, 466, 467
BcoDI GTCTC 1 cut(s) 633
BfmI CTRYAG 2 cut(s) 369, 944
BisI GCNGC 5 cut(s) 5, 175, 208, 277, 403
BlsI GCNGC 5 cut(s) 6, 176, 209, 278, 404
Bme1390I CCNGG 3 cut(s) 419, 466, 467
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 897
BmiI GGNNCC 4 cut(s) 160, 238, 457, 463
BmrFI CCNGG 3 cut(s) 419, 466, 467
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 2 cut(s) 211, 574
BpuMI CCSGG 3 cut(s) 419, 466, 467
BsaJI CCNNGG 2 cut(s) 417, 465
BsaWI WCCGGW 3 cut(s) 161, 233, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 465, 735
Bse118I RCCGGY 2 cut(s) 239, 503
Bse1I ACTGG 7 cut(s) 112, 140, 357, 457, 591, 604, 729
BseDI CCNNGG 2 cut(s) 417, 465
BseGI GGATG 3 cut(s) 78, 96, 413
BseLI CCNNNNNNNGG 3 cut(s) 107, 465, 735
BseMII CTCAG 3 cut(s) 30, 225, 588
BseNI ACTGG 7 cut(s) 112, 140, 357, 457, 591, 604, 729
BseXI GCAGC 3 cut(s) 219, 288, 389
BshFI GGCC 4 cut(s) 14, 90, 177, 899
BshNI GGYRCC 2 cut(s) 158, 461
BsiHKAI GWGCWC 2 cut(s) 611, 933
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 7 cut(s) 162, 234, 240, 419, 459, 466, 504
BslI CCNNNNNNNGG 3 cut(s) 107, 465, 735
BsmAI GTCTC 1 cut(s) 633
BsmBI CGTCTC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 4 cut(s) 14, 90, 177, 899
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 2 cut(s) 611, 933
Bsp143I GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
BspACI CCGC 4 cut(s) 5, 129, 174, 879
BspANI GGCC 4 cut(s) 14, 90, 177, 899
BspCNI CTCAG 3 cut(s) 29, 224, 587
BspLI GGNNCC 4 cut(s) 160, 238, 457, 463
BspPI GGATC 8 cut(s) 290, 422, 450, 463, 597, 644, 768, 855
BspQI GCTCTTC 1 cut(s) 616
BspT107I GGYRCC 2 cut(s) 158, 461
BsrFI RCCGGY 2 cut(s) 239, 503
BsrI ACTGG 7 cut(s) 112, 140, 357, 457, 591, 604, 729
BssAI RCCGGY 2 cut(s) 239, 503
BssECI CCNNGG 2 cut(s) 417, 465
BssMI GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
BssSI CACGAG 1 cut(s) 892
Bst2BI CACGAG 1 cut(s) 892
Bst6I CTCTTC 1 cut(s) 616
BstAPI GCANNNNNTGC 2 cut(s) 92, 131
BstC8I GCNNGC 3 cut(s) 88, 127, 505
BstDEI CTNAG 3 cut(s) 16, 211, 574
BstF5I GGATG 3 cut(s) 78, 96, 413
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 7 cut(s) 298, 430, 458, 592, 652, 763, 850
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
BstMWI GCNNNNNNNGC 3 cut(s) 92, 131, 928
BstNSI RCATGY 2 cut(s) 129, 315
BstSCI CCNGG 3 cut(s) 417, 464, 465
BstSFI CTRYAG 2 cut(s) 369, 944
BstV1I GCAGC 3 cut(s) 219, 288, 389
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 4 cut(s) 295, 455, 589, 847
BstYI RGATCY 4 cut(s) 295, 455, 589, 847
BsuRI GGCC 4 cut(s) 14, 90, 177, 899
BtgZI GCGATG 1 cut(s) 19
BtsCI GGATG 3 cut(s) 78, 96, 413
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 6 cut(s) 105, 450, 584, 611, 736, 849
Cac8I GCNNGC 3 cut(s) 88, 127, 505
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 2 cut(s) 239, 503
Cfr13I GGNCC 1 cut(s) 897
Cfr9I CCCGGG 1 cut(s) 465
CseI GACGC 1 cut(s) 131
Csp6I GTAC 4 cut(s) 159, 462, 484, 826
CviAII CATG 5 cut(s) 92, 126, 304, 312, 322
CviQI GTAC 4 cut(s) 159, 462, 484, 826
DdeI CTNAG 3 cut(s) 16, 211, 574
DpnI GATC 7 cut(s) 297, 429, 457, 591, 651, 762, 849
DpnII GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
EciI GGCGGA 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 933
Eco53kI GAGCTC 1 cut(s) 931
Eco88I CYCGRG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 933
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 5 cut(s) 95, 129, 307, 315, 325
FatI CATG 5 cut(s) 91, 125, 303, 311, 321
Fnu4HI GCNGC 5 cut(s) 5, 175, 208, 277, 403
FokI GGATG 3 cut(s) 65, 83, 400
FriOI GRGCYC 1 cut(s) 933
Fsp4HI GCNGC 5 cut(s) 5, 175, 208, 277, 403
GlaI GCGC 1 cut(s) 528
GluI GCNGC 5 cut(s) 5, 175, 208, 277, 403
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 4 cut(s) 14, 90, 177, 899
HapII CCGG 7 cut(s) 162, 234, 240, 419, 459, 466, 504
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 529
Hin1II CATG 5 cut(s) 95, 129, 307, 315, 325
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 3 cut(s) 309, 796, 865
HindII GTYRAC 3 cut(s) 309, 796, 865
HinfI GANTC 2 cut(s) 554, 692
HpaI GTTAAC 2 cut(s) 796, 865
HpaII CCGG 7 cut(s) 162, 234, 240, 419, 459, 466, 504
Hpy166II GTNNAC 4 cut(s) 309, 628, 796, 865
Hpy188I TCNGA 4 cut(s) 19, 194, 559, 654
Hpy188III TCNNGA 3 cut(s) 59, 213, 851
Hpy8I GTNNAC 4 cut(s) 309, 628, 796, 865
Hpy99I CGWCG 2 cut(s) 13, 658
HpyAV CCTTC 1 cut(s) 638
HpyCH4V TGCA 8 cut(s) 134, 207, 325, 492, 584, 682, 714, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 131, 928
HpyF3I CTNAG 3 cut(s) 16, 211, 574
Hsp92II CATG 5 cut(s) 95, 129, 307, 315, 325
HspAI GCGC 1 cut(s) 527
KpnI GGTACC 2 cut(s) 162, 465
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
KspAI GTTAAC 2 cut(s) 796, 865
Kzo9I GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
LguI GCTCTTC 1 cut(s) 616
LmnI GCTCC 2 cut(s) 606, 936
Lsp1109I GCAGC 3 cut(s) 219, 288, 389
LweI GCATC 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 289, 446
MalI GATC 7 cut(s) 297, 429, 457, 591, 651, 762, 849
MboI GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
MboII GAAGA 5 cut(s) 37, 379, 449, 584, 603
MflI RGATCY 4 cut(s) 295, 455, 589, 847
MhlI GDGCHC 2 cut(s) 611, 933
MluCI AATT 4 cut(s) 185, 224, 328, 808
MmeI TCCRAC 1 cut(s) 677
MroNI GCCGGC 1 cut(s) 503
MseI TTAA 2 cut(s) 795, 864
MslI CAYNNNNRTG 1 cut(s) 110
MspI CCGG 7 cut(s) 162, 234, 240, 419, 459, 466, 504
MspR9I CCNGG 3 cut(s) 419, 466, 467
Mva1269I GAATGC 1 cut(s) 529
MwoI GCNNNNNNNGC 3 cut(s) 92, 131, 928
NaeI GCCGGC 1 cut(s) 505
NciI CCSGG 3 cut(s) 419, 466, 467
NdeII GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 5 cut(s) 95, 129, 307, 315, 325
NlaIV GGNNCC 4 cut(s) 160, 238, 457, 463
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 2 cut(s) 289, 446
NspI RCATGY 2 cut(s) 129, 315
PaeI GCATGC 1 cut(s) 129
PciI ACATGT 1 cut(s) 311
PciSI GCTCTTC 1 cut(s) 616
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 554, 692
PkrI GCNGC 5 cut(s) 6, 176, 209, 278, 404
PscI ACATGT 1 cut(s) 311
Psp124BI GAGCTC 1 cut(s) 933
PspN4I GGNNCC 4 cut(s) 160, 238, 457, 463
PspPI GGNCC 1 cut(s) 897
PsuI RGATCY 4 cut(s) 295, 455, 589, 847
RsaI GTAC 4 cut(s) 160, 463, 485, 827
RsaNI GTAC 4 cut(s) 159, 462, 484, 826
RseI CAYNNNNRTG 1 cut(s) 110
SacI GAGCTC 1 cut(s) 933
SapI GCTCTTC 1 cut(s) 616
SaqAI TTAA 2 cut(s) 795, 864
SatI GCNGC 5 cut(s) 5, 175, 208, 277, 403
Sau3AI GATC 7 cut(s) 295, 427, 455, 589, 649, 760, 847
Sau96I GGNCC 1 cut(s) 897
ScrFI CCNGG 3 cut(s) 419, 466, 467
SduI GDGCHC 2 cut(s) 611, 933
SetI ASST 8 cut(s) 212, 337, 347, 542, 580, 603, 933, 942
SfaNI GCATC 1 cut(s) 252
SfcI CTRYAG 2 cut(s) 369, 944
SmaI CCCGGG 1 cut(s) 467
SmiMI CAYNNNNRTG 1 cut(s) 110
SphI GCATGC 1 cut(s) 129
Sse9I AATT 4 cut(s) 185, 224, 328, 808
SsiI CCGC 4 cut(s) 5, 129, 174, 879
SstI GAGCTC 1 cut(s) 933
StyD4I CCNGG 3 cut(s) 417, 464, 465
TasI AATT 4 cut(s) 185, 224, 328, 808
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 7, 177
TfiI GAWTC 2 cut(s) 554, 692
Tru1I TTAA 2 cut(s) 795, 864
Tru9I TTAA 2 cut(s) 795, 864
TscAI CASTG 6 cut(s) 112, 457, 591, 611, 736, 849
TseFI GTSAC 2 cut(s) 289, 446
TseI GCWGC 3 cut(s) 207, 276, 402
Tsp45I GTSAC 2 cut(s) 289, 446
TspGWI ACGGA 1 cut(s) 64
TspMI CCCGGG 1 cut(s) 465
TspRI CASTG 6 cut(s) 112, 457, 591, 611, 736, 849
XapI RAATTY 2 cut(s) 185, 328
XceI RCATGY 2 cut(s) 129, 315
XmaI CCCGGG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.