Rw0G016420

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00751
OG0015607
Physical Location & Seq
Reverse (-)
318376 .. 319890
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G016420.1

Sequence Viewer

Length: 915 bp
ATGCCGTCTGCCTCAGAAACCTTGCTCCTCTCTACCATTTTCCTCTTGCTAACTCCTCCGATCCACACCGCCTCTTCCATAATCTACAAGACATGCCGTGACACATGCGGCACAATCCCTGTCAAGTTCCCATTCGGCACCGGATTCGGCTGCGGCCACCCGGACTTCACCAGGTACATCAAATGCACCAACCTAGACACTCTTCAGTTCACCACCGGAACCGGCATTTACACCATTTCCTCCATAGACTACACCAGCAACACTATCATAGTCGCAGACCCGCTAATGTCAACATGCTCCTCCATGCAAAACTCCGGTAGCTTCAGCTTGGACAAGGCCAGCCCTTTCACTATAAGAGATGAGAACATCTTTGTTCTTCTCGGCTGCTCCACAACATCTCCTGTTTTCGATCCAAGTGAAGATCTGTGTAACACCGGCTCGGCTTCTCGGGTTTGTAGAGGGTTATACTCCTGCAAAGGGGTGTCTGGAATAGGGTTGCAGCAGAATGCGCCGATATCTACTTGTTGTGTCTATGATGATTCTGTTGCTGGGTTCGGGTCGGGTTATGCGTTGGATCTTCCCAAGCTGCAATGCTCGTCTTATACATCGGTTTATGAGTTTGGAGACGAAGGGGATCCGACGAAATGGAAATTTGGGATTTCTTTGCAGTATAATGATTCTTATTATACTAGACCCTGCAAGGATTGTGAGGCGAGTGGAGGGTTGTGTGGCTTTGCTGCTACGGATCAGTCTTTTGCTTGTATATGCCGGAGTGGGATGAACACCACCACAAATTGCTTTGGTCAAGGGTCTGCTTGGAGTGGGAGTGGGACATTGGAGCCCAGAGTTCAAATTAAAACCATTATTGGAGGATTTTTGGTCCCATGGGTCTTACTATTTGTAAGGAGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

304

Amino Acids

32.64

Weight (kDa)

5.18

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 32 - 93 1.1e-11 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 219 - 258 1.3e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015607)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30030 FvH4_4g30030
malus_domestica MD13G1062800.v1.1
prunus_persica Prupe.1G286900_v2.0.a1
pyrus_communis pycom13g05520
rosa_chinensis RchiOBHm_Chr4g0438321
rosa_laevigata RLG00000006370
rosa_multiflora Rmu_sc0011129.1_g000001
rosa_roxburghii Rroxscaffold_5G00379410
rosa_rugosa Rorug04G0309000
rosa_samantha Rh4AG360100 Rh4BG371100 Rh4CG385100 Rh4DG366000
rosa_wichuraiana Rw0G006470 Rw0G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AciI CCGC 4 cut(s) 69, 108, 153, 281
AclWI GGATC 6 cut(s) 55, 404, 582, 629, 642, 753
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 1 cut(s) 650
AcuI CTGAAG 2 cut(s) 188, 307
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 1 cut(s) 477
AgsI TTSAA 1 cut(s) 851
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 3 cut(s) 321, 327, 586
AluI AGCT 3 cut(s) 321, 327, 586
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 6 cut(s) 55, 404, 582, 629, 642, 753
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 2 cut(s) 154, 336
ApeKI GCWGC 5 cut(s) 150, 384, 499, 586, 737
ApoI RAATTY 1 cut(s) 650
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 1 cut(s) 880
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 160, 202
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 1 cut(s) 880
BamHI GGATCC 1 cut(s) 634
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 843
BbvI GCAGC 5 cut(s) 137, 371, 511, 573, 724
BceAI ACGGC 1 cut(s) 81
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 172
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 618
BfaI CTAG 2 cut(s) 194, 690
BglII AGATCT 1 cut(s) 421
BisI GCNGC 7 cut(s) 109, 151, 154, 385, 500, 587, 738
BlsI GCNGC 7 cut(s) 110, 152, 155, 386, 501, 588, 739
Bme1390I CCNGG 2 cut(s) 161, 172
Bme18I GGWCC 1 cut(s) 880
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 880
BmiI GGNNCC 5 cut(s) 139, 220, 636, 840, 882
BmrFI CCNGG 2 cut(s) 161, 172
BpuMI CCSGG 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 884
BsaWI WCCGGW 3 cut(s) 140, 215, 314
BsaXI ACNNNNNCTCC 4 cut(s) 382, 412, 811, 841
Bsc4I CCNNNNNNNGG 1 cut(s) 477
Bse118I RCCGGY 2 cut(s) 221, 434
Bse3DI GCAATG 1 cut(s) 596
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 884
BseGI GGATG 1 cut(s) 783
BseLI CCNNNNNNNGG 1 cut(s) 477
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 3 cut(s) 17, 45, 289
BseXI GCAGC 5 cut(s) 137, 371, 511, 573, 724
BseYI CCCAGC 1 cut(s) 548
BshFI GGCC 2 cut(s) 156, 338
BshNI GGYRCC 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 7 cut(s) 141, 161, 216, 222, 315, 435, 769
BslFI GGGAC 2 cut(s) 844, 866
BslI CCNNNNNNNGG 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 618
BsmBI CGTCTC 1 cut(s) 618
BsmFI GGGAC 2 cut(s) 844, 866
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 2 cut(s) 156, 338
BsoBI CYCGRG 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 6 cut(s) 60, 409, 421, 574, 634, 745
Bsp19I CCATGG 1 cut(s) 884
BspACI CCGC 4 cut(s) 69, 108, 153, 281
BspANI GGCC 2 cut(s) 156, 338
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 5 cut(s) 139, 220, 636, 840, 882
BspPI GGATC 6 cut(s) 55, 404, 582, 629, 642, 753
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 2 cut(s) 221, 434
BssAI RCCGGY 2 cut(s) 221, 434
BssECI CCNNGG 1 cut(s) 884
BssMI GATC 6 cut(s) 60, 409, 421, 574, 634, 745
BssT1I CCWWGG 1 cut(s) 884
Bst2UI CCWGG 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 79, 207
BstC8I GCNNGC 1 cut(s) 340
BstDEI CTNAG 1 cut(s) 13
BstDSI CCRYGG 1 cut(s) 884
BstF5I GGATG 1 cut(s) 783
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 6 cut(s) 63, 412, 424, 577, 637, 748
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 6 cut(s) 60, 409, 421, 574, 634, 745
BstMWI GCNNNNNNNGC 1 cut(s) 508
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 3 cut(s) 96, 108, 297
BstSCI CCNGG 2 cut(s) 159, 170
BstV1I GCAGC 5 cut(s) 137, 371, 511, 573, 724
BstX2I RGATCY 3 cut(s) 421, 574, 634
BstYI RGATCY 3 cut(s) 421, 574, 634
BsuRI GGCC 2 cut(s) 156, 338
BtgI CCRYGG 1 cut(s) 884
BtsCI GGATG 1 cut(s) 783
Cac8I GCNNGC 1 cut(s) 340
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 2 cut(s) 221, 434
Cfr13I GGNCC 1 cut(s) 880
CsiI ACCWGGT 1 cut(s) 170
Csp6I GTAC 1 cut(s) 175
CviAII CATG 5 cut(s) 93, 105, 294, 304, 885
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 13
DpnI GATC 6 cut(s) 62, 411, 423, 576, 636, 747
DpnII GATC 6 cut(s) 60, 409, 421, 574, 634, 745
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 2 cut(s) 79, 207
EarI CTCTTC 2 cut(s) 79, 207
Eco130I CCWWGG 1 cut(s) 884
Eco24I GRGCYC 1 cut(s) 843
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 880
Eco57I CTGAAG 2 cut(s) 188, 307
Eco88I CYCGRG 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 170
EcoRV GATATC 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 884
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 884
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 5 cut(s) 96, 108, 297, 307, 888
FaqI GGGAC 2 cut(s) 844, 866
FatI CATG 5 cut(s) 92, 104, 293, 303, 884
FauI CCCGC 1 cut(s) 288
Fnu4HI GCNGC 7 cut(s) 109, 151, 154, 385, 500, 587, 738
FokI GGATG 1 cut(s) 790
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 7 cut(s) 109, 151, 154, 385, 500, 587, 738
FspBI CTAG 2 cut(s) 194, 690
GlaI GCGC 1 cut(s) 510
GluI GCNGC 7 cut(s) 109, 151, 154, 385, 500, 587, 738
GsaI CCCAGC 1 cut(s) 552
HaeIII GGCC 2 cut(s) 156, 338
HapII CCGG 7 cut(s) 141, 161, 216, 222, 315, 435, 769
HhaI GCGC 1 cut(s) 511
Hin1II CATG 5 cut(s) 96, 108, 297, 307, 888
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 3 cut(s) 144, 539, 677
HpaII CCGG 7 cut(s) 141, 161, 216, 222, 315, 435, 769
HphI GGTGA 2 cut(s) 160, 202
Hpy166II GTNNAC 2 cut(s) 210, 291
Hpy188I TCNGA 3 cut(s) 16, 60, 639
Hpy188III TCNNGA 1 cut(s) 486
Hpy8I GTNNAC 2 cut(s) 210, 291
Hpy99I CGWCG 1 cut(s) 643
HpyAV CCTTC 1 cut(s) 623
HpyCH4V TGCA 7 cut(s) 186, 307, 474, 499, 589, 667, 699
HpyF10VI GCNNNNNNNGC 1 cut(s) 508
HpyF3I CTNAG 1 cut(s) 13
Hsp92II CATG 5 cut(s) 96, 108, 297, 307, 888
HspAI GCGC 1 cut(s) 509
Kzo9I GATC 6 cut(s) 60, 409, 421, 574, 634, 745
LmnI GCTCC 5 cut(s) 30, 302, 392, 838, 906
Lsp1109I GCAGC 5 cut(s) 137, 371, 511, 573, 724
MabI ACCWGGT 1 cut(s) 170
MaeI CTAG 2 cut(s) 194, 690
MaeIII GTNAC 2 cut(s) 98, 428
MalI GATC 6 cut(s) 62, 411, 423, 576, 636, 747
MboI GATC 6 cut(s) 60, 409, 421, 574, 634, 745
MboII GAAGA 5 cut(s) 66, 194, 368, 431, 569
MflI RGATCY 3 cut(s) 421, 574, 634
MhlI GDGCHC 1 cut(s) 843
MluCI AATT 3 cut(s) 650, 793, 852
MmeI TCCRAC 2 cut(s) 552, 662
MseI TTAA 1 cut(s) 855
MspI CCGG 7 cut(s) 141, 161, 216, 222, 315, 435, 769
MspR9I CCNGG 2 cut(s) 161, 172
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 508
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 1 cut(s) 884
NdeII GATC 6 cut(s) 60, 409, 421, 574, 634, 745
NlaIII CATG 5 cut(s) 96, 108, 297, 307, 888
NlaIV GGNNCC 5 cut(s) 139, 220, 636, 840, 882
NmeAIII GCCGAG 2 cut(s) 360, 419
NmuCI GTSAC 1 cut(s) 98
NspI RCATGY 3 cut(s) 96, 108, 297
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PctI GAATGC 1 cut(s) 511
PfeI GAWTC 3 cut(s) 144, 539, 677
PkrI GCNGC 7 cut(s) 110, 152, 155, 386, 501, 588, 739
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 5 cut(s) 139, 220, 636, 840, 882
PspPI GGNCC 1 cut(s) 880
PsuI RGATCY 3 cut(s) 421, 574, 634
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 855
SatI GCNGC 7 cut(s) 109, 151, 154, 385, 500, 587, 738
Sau3AI GATC 6 cut(s) 60, 409, 421, 574, 634, 745
Sau96I GGNCC 1 cut(s) 880
ScrFI CCNGG 2 cut(s) 161, 172
SduI GDGCHC 1 cut(s) 843
SetI ASST 6 cut(s) 23, 176, 195, 323, 329, 588
SexAI ACCWGGT 1 cut(s) 170
SinI GGWCC 1 cut(s) 880
Sse9I AATT 3 cut(s) 650, 793, 852
SsiI CCGC 4 cut(s) 69, 108, 153, 281
SspMI CTAG 2 cut(s) 194, 690
StyD4I CCNGG 2 cut(s) 159, 170
StyI CCWWGG 1 cut(s) 884
TaqI TCGA 1 cut(s) 408
TasI AATT 3 cut(s) 650, 793, 852
TauI GCSGC 2 cut(s) 111, 156
TfiI GAWTC 3 cut(s) 144, 539, 677
Tru1I TTAA 1 cut(s) 855
Tru9I TTAA 1 cut(s) 855
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 5 cut(s) 150, 384, 499, 586, 737
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 1 cut(s) 794
TspGWI ACGGA 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 880
XapI RAATTY 1 cut(s) 650
XceI RCATGY 3 cut(s) 96, 108, 297
XspI CTAG 2 cut(s) 194, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.