MD13G1100800.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007325
Physical Location & Seq
Forward (+)
7133912 .. 7137568
3657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1100800.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGAAGTTTATGAAACTTGGATCCCGGCAGGATACTTTCTACACTACTGAGGCTGTAAGGTCAGTTTCCTCTGAAGTTTCAAGCGATCTCATAATTCAAGTGAAAGGAAGCAGATATTTGCTTCACAAGTTTCCTCTGTTATCCAAGTGTCTGCGGCTGCAGAGGCTTTGCTCTGAGTACCAAGAATCTTCAAACCACCACATAATCCATCTCCACGACTTCCCCTGTGGCGTCGAGGCTTTCGAGCTCTGCGCAAAGTTCTGCTACGGCATCACAATCACTCTCAGCGCCTACAACATTGTCGCTTCTCGCTGTGCCGCCGAGTACCTGCAGATGACCGAGGATGTCGAGAAGGGAAACCTTATATACAAACTCGAGGTCTTCTTCAACTCCTGCATCCTCCACGGTTGGAGGGATTCAATTGCCACGCTTCAGAGCACAAAGGCATTTCCTCTGTGGTCGGAGGAGCTCGGAATCACATCCAGGTGCATTGAAGCAATTGCCACAAAAGTCCTCACAAACCCTTCCAAAGTTAGCCTGTCACACAGCTACTCCAGGAGGGGCAGAGACGACATTGTGTCTTGCAATGGCGCCGAGAGCCTCCGGCATAACAGCAGGCCAGAAGGCAGGGGATGGTGGGCTGAGGACATTGCAGAATTGGGGATTGACTTGTACTGGAGAACTATGATTGCAATAAAATCTGGTGGGAAAATTCCCTCTAGTCTCATTGGAGATGCATTGAAAATTTATGCTGCTCGATGGTTGCCTAAAATATCGAAAAACGACAGTGATCATCGACAAGCAGCGTTGGATTCCGATTCAGACTCTGCCAATGATTTAACCTCAAAGCATAGGTTGATCTTGGAGTCCATTGTAAGTTTAGTTCCGGTTGAAAAATGTGCTGCTTCCACTAGCTTTCTGCTTAAACTTTTAAAGGCAGCCAACATTCTCAATGCTTCTTCTTCTTCAAAAATGGAGTTGGCCAGAAGGGTAGGGCTTCAATTGGATGATGCAACAGTCAGTGACTTGCTAATCCCCTCTGAGTCATACGAAAGCACCACGCGATATGATGTGGACATAGTCATGACAATTTTGGAGCAGTTCTTGCTGCAGGGGCAGAGTCCCCCAACTAGTCCTCCGAGGTCCAAGCTTGGCTTTGAAAGGAGGAGGAGGTCTCGCTCTGCGGAGAACATTGATTTTGAGTTTCAGGAAAGCCGGAGATCTTCCTCGGCGTCACATAGCTCGAAGCTGAAGGTGGCAAAGATTATGGATGTATATCTTAAGGAGATTGCCAGGGATGTCAATTTGTCCCTCTCAAAGTTCATTGCAATTGCTGAAGCAATACCAGAGTTTGCAAGGCTTGACCATGACGATCTCTACAGAGCAATTGACATTTACCTCAAGGCACATCCAGACCTAAACAAAAGTGAAAGGAAGAGGCTTTGCAGAATTCTAGACTGCAAAAAGCTCTCGATGGAGGCCTGCATGCATGCTGCACAAAATGAGCTACTCCCACTAAGAGTGGTGGTCCAAGTTCTCTTCTTTGAGCAAGCCCGAGCCGCCATGGCCAGCGGCAAAGTGACCGAGCTGCCTAGCAACATTAGGGAACTGCTAGCGTCACATAACATTGATCCGTCGAGGCCTCCGCCCTCGCTAAGCAACGCTAGCATTGTTCCGGCAGAGGACCAATGGAGCGCTTCAGGCCTGAAATCACCAAAGTCGAGGATGTCAACTTTACAAATGAAGCTGGCGGAAGATGATGATTTGGACGAAAATAATATGCACCCGGATGGAATTGGGAGAAACTCAAAGATGAAATCTCTTTGCTCACTTCCTACTAGACCCAAAAGAATGTTGAGTAAGTTGTGGTCCATCAACAGAAGTAGCTGTGAAAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

634

Amino Acids

71.05

Weight (kDa)

8.65

Isoelectric Point (pI)

61.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 212 - 495 2.7e-92 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 336
AccB1I GGYRCC 1 cut(s) 590
AccII CGCG 1 cut(s) 1063
AciI CCGC 7 cut(s) 154, 318, 1184, 1560, 1572, 1646, 1751
AclWI GGATC 3 cut(s) 15, 28, 1625
AcoI YGGCCR 2 cut(s) 981, 1566
AcsI RAATTY 3 cut(s) 711, 744, 1449
AcuI CTGAAG 5 cut(s) 93, 416, 1271, 1356, 1683
AcyI GRCGYC 3 cut(s) 231, 591, 1232
AfaI GTAC 3 cut(s) 179, 326, 674
AfeI AGCGCT 1 cut(s) 1696
AflII CTTAAG 1 cut(s) 1280
AhdI GACNNNNNGTC 1 cut(s) 577
AhlI ACTAGT 1 cut(s) 1130
AjnI CCWGG 3 cut(s) 482, 554, 1292
Alw21I GWGCWC 3 cut(s) 249, 440, 471
Alw26I GTCTC 3 cut(s) 561, 728, 1179
AlwI GGATC 3 cut(s) 15, 28, 1625
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 2 cut(s) 374, 1554
Aor51HI AGCGCT 1 cut(s) 1696
AoxI GGCC 6 cut(s) 617, 981, 1479, 1566, 1640, 1702
ApeKI GCWGC 8 cut(s) 157, 752, 803, 902, 938, 1108, 1493, 1588
ApoI RAATTY 3 cut(s) 711, 744, 1449
ArsI GACNNNNNNTTYG 2 cut(s) 363, 395
AspLEI GCGC 4 cut(s) 254, 290, 593, 1697
AspS9I GGNCC 4 cut(s) 1143, 1528, 1684, 1869
AsuC2I CCSGG 2 cut(s) 25, 1787
AsuHPI GGTGA 1 cut(s) 1704
AsuNHI GCTAGC 2 cut(s) 1612, 1664
AvaI CYCGRG 2 cut(s) 374, 1554
AvaII GGWCC 4 cut(s) 1143, 1528, 1684, 1869
BalI TGGCCA 2 cut(s) 983, 1568
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 590
BanII GRGCYC 2 cut(s) 249, 471
BbsI GAAGAC 1 cut(s) 373
Bbv12I GWGCWC 3 cut(s) 249, 440, 471
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 8 cut(s) 144, 739, 815, 889, 950, 1095, 1480, 1575
BccI CCATC 6 cut(s) 216, 627, 753, 1468, 1784, 1880
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 3 cut(s) 484, 556, 1294
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 2 cut(s) 25, 1787
BcoDI GTCTC 3 cut(s) 561, 728, 1179
BcuI ACTAGT 1 cut(s) 1130
BfaI CTAG 8 cut(s) 720, 912, 1131, 1454, 1593, 1613, 1665, 1839
BfmI CTRYAG 4 cut(s) 158, 329, 1109, 1378
BfoI RGCGCY 3 cut(s) 291, 594, 1698
BfrI CTTAAG 1 cut(s) 1280
BfuAI ACCTGC 1 cut(s) 336
BfuI GTATCC 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 1565
BglII AGATCT 1 cut(s) 1220
BlpI GCTNAGC 1 cut(s) 1655
Bme1390I CCNGG 5 cut(s) 25, 484, 556, 1294, 1787
Bme18I GGWCC 4 cut(s) 1143, 1528, 1684, 1869
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 2 cut(s) 374, 1554
BmgT120I GGNCC 4 cut(s) 1143, 1528, 1684, 1869
BmiI GGNNCC 2 cut(s) 22, 592
BmrFI CCNGG 5 cut(s) 25, 484, 556, 1294, 1787
BmsI GCATC 4 cut(s) 279, 405, 724, 1000
BmtI GCTAGC 2 cut(s) 1616, 1668
BpiI GAAGAC 1 cut(s) 373
BplI GAGNNNNNCTC 2 cut(s) 1159, 1191
BpmI CTGGAG 2 cut(s) 538, 697
Bpu10I CCTNAGC 1 cut(s) 642
Bpu1102I GCTNAGC 1 cut(s) 1655
BpuEI CTTGAG 1 cut(s) 1385
BpuMI CCSGG 2 cut(s) 25, 1787
BsaBI GATNNNNATC 1 cut(s) 1275
BsaHI GRCGYC 3 cut(s) 231, 591, 1232
BsaI GGTCTC 1 cut(s) 1179
BsaJI CCNNGG 6 cut(s) 339, 403, 1139, 1227, 1293, 1563
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 4 cut(s) 536, 566, 1120, 1150
Bse1I ACTGG 1 cut(s) 680
Bse3DI GCAATG 3 cut(s) 592, 648, 1323
Bse8I GATNNNNATC 1 cut(s) 1275
BseBI CCWGG 3 cut(s) 484, 556, 1294
BseDI CCNNGG 6 cut(s) 339, 403, 1139, 1227, 1293, 1563
BseJI GATNNNNATC 1 cut(s) 1275
BseMI GCAATG 3 cut(s) 592, 648, 1323
BseMII CTCAG 5 cut(s) 39, 165, 298, 633, 1032
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 3 cut(s) 479, 1180, 1183
BseXI GCAGC 8 cut(s) 144, 739, 815, 889, 950, 1095, 1480, 1575
BsgI GTGCAG 1 cut(s) 1479
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 6 cut(s) 619, 983, 1481, 1568, 1642, 1704
BshNI GGYRCC 1 cut(s) 590
BsiHKAI GWGCWC 3 cut(s) 249, 440, 471
BsiHKCI CYCGRG 2 cut(s) 374, 1554
BsiSI CCGG 6 cut(s) 25, 604, 887, 1216, 1676, 1787
BslFI GGGAC 2 cut(s) 1107, 1294
BsmAI GTCTC 3 cut(s) 561, 728, 1179
BsmBI CGTCTC 1 cut(s) 561
BsmFI GGGAC 2 cut(s) 1107, 1294
BsnI GGCC 6 cut(s) 619, 983, 1481, 1568, 1642, 1704
Bso31I GGTCTC 1 cut(s) 1179
BsoBI CYCGRG 2 cut(s) 374, 1554
Bsp1286I GDGCHC 3 cut(s) 249, 440, 471
Bsp143I GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
Bsp1720I GCTNAGC 1 cut(s) 1655
Bsp19I CCATGG 1 cut(s) 1563
BspACI CCGC 7 cut(s) 154, 318, 1184, 1560, 1572, 1646, 1751
BspANI GGCC 6 cut(s) 619, 983, 1481, 1568, 1642, 1704
BspCNI CTCAG 5 cut(s) 40, 166, 297, 634, 1033
BspFNI CGCG 1 cut(s) 1063
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 2 cut(s) 22, 592
BspMAI CTGCAG 3 cut(s) 162, 333, 1113
BspMI ACCTGC 1 cut(s) 336
BspOI GCTAGC 2 cut(s) 1616, 1668
BspPI GGATC 3 cut(s) 15, 28, 1625
BspT107I GGYRCC 1 cut(s) 590
BspTI CTTAAG 1 cut(s) 1280
BspTNI GGTCTC 1 cut(s) 1179
BsrDI GCAATG 3 cut(s) 592, 648, 1323
BsrI ACTGG 1 cut(s) 680
BssECI CCNNGG 6 cut(s) 339, 403, 1139, 1227, 1293, 1563
BssMI GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
BssNI GRCGYC 3 cut(s) 231, 591, 1232
BssT1I CCWWGG 1 cut(s) 1563
Bst2UI CCWGG 3 cut(s) 484, 556, 1294
Bst4CI ACNGT 3 cut(s) 407, 788, 1018
Bst6I CTCTTC 2 cut(s) 1430, 1544
BstACI GRCGYC 3 cut(s) 231, 591, 1232
BstAFI CTTAAG 1 cut(s) 1280
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstC8I GCNNGC 9 cut(s) 617, 1483, 1487, 1491, 1551, 1570, 1614, 1666, 1749
BstDEI CTNAG 7 cut(s) 48, 174, 284, 642, 1041, 1517, 1655
BstDSI CCRYGG 2 cut(s) 403, 1563
BstFNI CGCG 1 cut(s) 1063
BstH2I RGCGCY 3 cut(s) 291, 594, 1698
BstHHI GCGC 4 cut(s) 254, 290, 593, 1697
BstKTI GATC 7 cut(s) 23, 88, 793, 861, 1223, 1375, 1633
BstMAI GTCTC 3 cut(s) 561, 728, 1179
BstMBI GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
BstMWI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1559, 1565, 1665, 1701
BstNI CCWGG 3 cut(s) 484, 556, 1294
BstNSI RCATGY 2 cut(s) 1489, 1493
BstSCI CCNGG 5 cut(s) 23, 482, 554, 1292, 1785
BstSFI CTRYAG 4 cut(s) 158, 329, 1109, 1378
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 8 cut(s) 144, 739, 815, 889, 950, 1095, 1480, 1575
BstV2I GAAGAC 1 cut(s) 373
BstX2I RGATCY 2 cut(s) 20, 1220
BstYI RGATCY 2 cut(s) 20, 1220
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 6 cut(s) 619, 983, 1481, 1568, 1642, 1704
BtgI CCRYGG 2 cut(s) 403, 1563
BtsIMutI CAGTG 2 cut(s) 793, 1027
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 9 cut(s) 617, 1483, 1487, 1491, 1551, 1570, 1614, 1666, 1749
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 1083
CfoI GCGC 4 cut(s) 254, 290, 593, 1697
Cfr13I GGNCC 4 cut(s) 1143, 1528, 1684, 1869
CseI GACGC 3 cut(s) 220, 1221, 1605
Csp6I GTAC 3 cut(s) 178, 325, 673
CviAII CATG 5 cut(s) 1084, 1367, 1486, 1490, 1564
CviQI GTAC 3 cut(s) 178, 325, 673
DdeI CTNAG 7 cut(s) 48, 174, 284, 642, 1041, 1517, 1655
DinI GGCGCC 1 cut(s) 592
DpnI GATC 7 cut(s) 22, 87, 792, 860, 1222, 1374, 1632
DpnII GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
DraI TTTAAA 1 cut(s) 933
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 2 cut(s) 981, 1566
Eam1104I CTCTTC 2 cut(s) 1430, 1544
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 2 cut(s) 1430, 1544
EciI GGCGGA 2 cut(s) 1635, 1766
Ecl136II GAGCTC 2 cut(s) 247, 469
Eco130I CCWWGG 1 cut(s) 1563
Eco147I AGGCCT 3 cut(s) 1481, 1642, 1704
Eco24I GRGCYC 2 cut(s) 249, 471
Eco31I GGTCTC 1 cut(s) 1179
Eco47I GGWCC 4 cut(s) 1143, 1528, 1684, 1869
Eco47III AGCGCT 1 cut(s) 1696
Eco53kI GAGCTC 2 cut(s) 247, 469
Eco57I CTGAAG 5 cut(s) 93, 416, 1271, 1356, 1683
Eco88I CYCGRG 2 cut(s) 374, 1554
EcoICRI GAGCTC 2 cut(s) 247, 469
EcoRI GAATTC 1 cut(s) 1449
EcoRII CCWGG 3 cut(s) 482, 554, 1292
EcoT14I CCWWGG 1 cut(s) 1563
EcoT22I ATGCAT 2 cut(s) 739, 1491
EcoT38I GRGCYC 2 cut(s) 249, 471
EgeI GGCGCC 1 cut(s) 592
EheI GGCGCC 1 cut(s) 592
ErhI CCWWGG 1 cut(s) 1563
Esp3I CGTCTC 1 cut(s) 561
FaeI CATG 5 cut(s) 1087, 1370, 1489, 1493, 1567
FalI AAGNNNNNCTT 2 cut(s) 1139, 1171
FaqI GGGAC 2 cut(s) 1107, 1294
FatI CATG 5 cut(s) 1083, 1366, 1485, 1489, 1563
FbaI TGATCA 1 cut(s) 790
FriOI GRGCYC 2 cut(s) 249, 471
FspBI CTAG 8 cut(s) 720, 912, 1131, 1454, 1593, 1613, 1665, 1839
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 4 cut(s) 253, 289, 592, 1696
GsuI CTGGAG 2 cut(s) 538, 697
HaeII RGCGCY 3 cut(s) 291, 594, 1698
HaeIII GGCC 6 cut(s) 619, 983, 1481, 1568, 1642, 1704
HapII CCGG 6 cut(s) 25, 604, 887, 1216, 1676, 1787
HgaI GACGC 3 cut(s) 220, 1221, 1605
HhaI GCGC 4 cut(s) 254, 290, 593, 1697
Hin1I GRCGYC 3 cut(s) 231, 591, 1232
Hin1II CATG 5 cut(s) 1087, 1370, 1489, 1493, 1567
Hin6I GCGC 4 cut(s) 252, 288, 591, 1695
HinP1I GCGC 4 cut(s) 252, 288, 591, 1695
HincII GTYRAC 1 cut(s) 1731
HindII GTYRAC 1 cut(s) 1731
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 9 cut(s) 185, 416, 474, 812, 818, 824, 866, 1043, 1120
HpaII CCGG 6 cut(s) 25, 604, 887, 1216, 1676, 1787
HphI GGTGA 1 cut(s) 1704
Hpy166II GTNNAC 2 cut(s) 1075, 1731
Hpy188I TCNGA 9 cut(s) 73, 175, 435, 463, 473, 817, 823, 1042, 1140
Hpy188III TCNNGA 6 cut(s) 349, 1084, 1208, 1412, 1454, 1471
Hpy8I GTNNAC 2 cut(s) 1075, 1731
Hpy99I CGWCG 2 cut(s) 236, 1639
HpyAV CCTTC 5 cut(s) 346, 534, 617, 981, 1246
HpyCH4III ACNGT 3 cut(s) 407, 788, 1018
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1559, 1565, 1665, 1701
HpyF3I CTNAG 7 cut(s) 48, 174, 284, 642, 1041, 1517, 1655
Hsp92I GRCGYC 3 cut(s) 231, 591, 1232
Hsp92II CATG 5 cut(s) 1087, 1370, 1489, 1493, 1567
HspAI GCGC 4 cut(s) 252, 288, 591, 1695
KasI GGCGCC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
LmnI GCTCC 3 cut(s) 466, 1096, 1692
Lsp1109I GCAGC 8 cut(s) 144, 739, 815, 889, 950, 1095, 1480, 1575
LweI GCATC 4 cut(s) 279, 405, 724, 1000
MaeI CTAG 8 cut(s) 720, 912, 1131, 1454, 1593, 1613, 1665, 1839
MaeIII GTNAC 5 cut(s) 540, 1022, 1233, 1579, 1617
MalI GATC 7 cut(s) 22, 87, 792, 860, 1222, 1374, 1632
MboI GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
MfeI CAATTG 5 cut(s) 420, 498, 1001, 1329, 1386
MflI RGATCY 2 cut(s) 20, 1220
MhlI GDGCHC 3 cut(s) 249, 440, 471
MlsI TGGCCA 2 cut(s) 983, 1568
MluNI TGGCCA 2 cut(s) 983, 1568
Mly113I GGCGCC 1 cut(s) 591
MlyI GAGTC 4 cut(s) 818, 875, 1052, 1129
MmeI TCCRAC 3 cut(s) 389, 441, 789
Mox20I TGGCCA 2 cut(s) 983, 1568
Mph1103I ATGCAT 2 cut(s) 739, 1491
MscI TGGCCA 2 cut(s) 983, 1568
MseI TTAA 4 cut(s) 839, 924, 932, 1281
MslI CAYNNNNRTG 3 cut(s) 484, 1082, 1788
Msp20I TGGCCA 2 cut(s) 983, 1568
MspA1I CMGCKG 1 cut(s) 1572
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 6 cut(s) 25, 604, 887, 1216, 1676, 1787
MspR9I CCNGG 5 cut(s) 25, 484, 556, 1294, 1787
MunI CAATTG 5 cut(s) 420, 498, 1001, 1329, 1386
MvaI CCWGG 3 cut(s) 484, 556, 1294
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1559, 1565, 1665, 1701
NarI GGCGCC 1 cut(s) 591
NciI CCSGG 2 cut(s) 25, 1787
NcoI CCATGG 1 cut(s) 1563
NdeII GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
NheI GCTAGC 2 cut(s) 1612, 1664
NlaIII CATG 5 cut(s) 1087, 1370, 1489, 1493, 1567
NlaIV GGNNCC 2 cut(s) 22, 592
NmeAIII GCCGAG 3 cut(s) 346, 619, 1208
NmuCI GTSAC 5 cut(s) 540, 1022, 1233, 1579, 1617
NsbI TGCGCA 1 cut(s) 253
NsiI ATGCAT 2 cut(s) 739, 1491
NspI RCATGY 2 cut(s) 1489, 1493
PaeI GCATGC 2 cut(s) 1489, 1493
PaeR7I CTCGAG 1 cut(s) 374
PagI TCATGA 1 cut(s) 1083
PceI AGGCCT 3 cut(s) 1481, 1642, 1704
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 5 cut(s) 185, 416, 474, 812, 818
PflFI GACNNNGTC 1 cut(s) 1079
PfoI TCCNGGA 1 cut(s) 554
PleI GAGTC 4 cut(s) 818, 874, 1051, 1128
PluTI GGCGCC 1 cut(s) 594
PpsI GAGTC 4 cut(s) 818, 874, 1051, 1128
Psp124BI GAGCTC 2 cut(s) 249, 471
Psp6I CCWGG 3 cut(s) 482, 554, 1292
PspGI CCWGG 3 cut(s) 482, 554, 1292
PspN4I GGNNCC 2 cut(s) 22, 592
PspPI GGNCC 4 cut(s) 1143, 1528, 1684, 1869
PspXI VCTCGAGB 1 cut(s) 374
PsrI GAACNNNNNNTAC 2 cut(s) 867, 899
PstI CTGCAG 3 cut(s) 162, 333, 1113
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 20, 1220
PsyI GACNNNGTC 1 cut(s) 1079
RsaI GTAC 3 cut(s) 179, 326, 674
RsaNI GTAC 3 cut(s) 178, 325, 673
RseI CAYNNNNRTG 3 cut(s) 484, 1082, 1788
SacI GAGCTC 2 cut(s) 249, 471
SaqAI TTAA 4 cut(s) 839, 924, 932, 1281
Sau3AI GATC 7 cut(s) 20, 85, 790, 858, 1220, 1372, 1630
Sau96I GGNCC 4 cut(s) 1143, 1528, 1684, 1869
SchI GAGTC 4 cut(s) 818, 875, 1052, 1129
ScrFI CCNGG 5 cut(s) 25, 484, 556, 1294, 1787
SduI GDGCHC 3 cut(s) 249, 440, 471
SfaNI GCATC 4 cut(s) 279, 405, 724, 1000
SfcI CTRYAG 4 cut(s) 158, 329, 1109, 1378
SfoI GGCGCC 1 cut(s) 592
Sfr274I CTCGAG 1 cut(s) 374
SinI GGWCC 4 cut(s) 1143, 1528, 1684, 1869
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 3 cut(s) 484, 1082, 1788
SmlI CTYRAG 3 cut(s) 374, 1280, 1400
SmoI CTYRAG 3 cut(s) 374, 1280, 1400
SpeI ACTAGT 1 cut(s) 1130
SphI GCATGC 2 cut(s) 1489, 1493
SseBI AGGCCT 3 cut(s) 1481, 1642, 1704
SsiI CCGC 7 cut(s) 154, 318, 1184, 1560, 1572, 1646, 1751
SspDI GGCGCC 1 cut(s) 590
SspMI CTAG 8 cut(s) 720, 912, 1131, 1454, 1593, 1613, 1665, 1839
SstI GAGCTC 2 cut(s) 249, 471
StuI AGGCCT 3 cut(s) 1481, 1642, 1704
StyD4I CCNGG 5 cut(s) 23, 482, 554, 1292, 1785
StyI CCWWGG 1 cut(s) 1563
TaaI ACNGT 3 cut(s) 407, 788, 1018
TaqII GACCGA 2 cut(s) 353, 1598
TatI WGTACW 1 cut(s) 672
TauI GCSGC 4 cut(s) 157, 320, 1562, 1575
TfiI GAWTC 5 cut(s) 185, 416, 474, 812, 818
Tru1I TTAA 4 cut(s) 839, 924, 932, 1281
Tru9I TTAA 4 cut(s) 839, 924, 932, 1281
TscAI CASTG 2 cut(s) 793, 1027
TseFI GTSAC 5 cut(s) 540, 1022, 1233, 1579, 1617
TseI GCWGC 8 cut(s) 157, 752, 803, 902, 938, 1108, 1493, 1588
Tsp45I GTSAC 5 cut(s) 540, 1022, 1233, 1579, 1617
TspDTI ATGAA 5 cut(s) 17, 26, 1312, 1757, 1829
TspGWI ACGGA 1 cut(s) 1623
TspRI CASTG 2 cut(s) 793, 1027
Tth111I GACNNNGTC 1 cut(s) 1079
Vha464I CTTAAG 1 cut(s) 1280
VpaK11BI GGWCC 4 cut(s) 1143, 1528, 1684, 1869
XapI RAATTY 3 cut(s) 711, 744, 1449
XbaI TCTAGA 1 cut(s) 1453
XceI RCATGY 2 cut(s) 1489, 1493
XhoI CTCGAG 1 cut(s) 374
XspI CTAG 8 cut(s) 720, 912, 1131, 1454, 1593, 1613, 1665, 1839
Zsp2I ATGCAT 2 cut(s) 739, 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.