MD16G1101200.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007325
Physical Location & Seq
Forward (+)
7020075 .. 7024024
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1101200.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGAAGTTTATGAAACTTGGATCCCGGCCGGATACTTTCTACACTACTGAGGCTGTAAGGTCAGTCTCCTCTGAAGTTTCAAGTGATCTCATAATTCAAGTGAAAGGAAGCAGATATTTGCTTCACAAGTTTCCTCTGTTATCCAAGTGTTTGAGGCTGCAGAGGCTCTGCTCTGAGTACCAAGAATCTTCAAACCACCACATAATCCAGCTCCATGACTTTCCCAGTAGCGTCGAGGCTTTCGAGCTCTGCGCAAAGTTCTGCTACGGCATCACTATCACTCTCAGCGCCTACAACATTGTCGCTTCTCGCTGTGCCGCTGAGTACCTGCAGATGACCGAGGACGTCGAGAAGGGAAACCTTATATACAAACTGGAGGTGTTCTTCAACTCCTGCATCCTCCACGGTTGGAGGGACTCAATTGCCACGCTTCAGAGCACAAAGGCATTTCCTCTGTGGTCGGAGGAGCTTGGAATCACATCGAGGTGCATTGAAGCAATTGCCACAAAAGTCCTCACAAACCCTTCCAAAGTTAGCCTGTCGCACAGCTATTCCAGGAGGGGCAGAGATGACATTGTGTCGTGCAATGGCTCCGAGAGCCTCCGGCATAATGGTAGGCCTGCAGGCAGGGGATGGTGGGCTGAGGACATTGCAGAATTGGGGATTGATTTGTATTGGAGAACTATGATTGCTATAAAATCTGGTGGGAAAATTCCCTCTAGTCTCATTGGAGATGCATTGAAAATTTATGCGGCTCGATGGTTGCCTAAAATATCAAAAAACGAATGTGATCATCGACAAGCAGCGTCGGATTCGGATTCAGACTCTGCCAATGATTTAACCTCAAAGCATAGGTTGATCTTGGAGTCCATCGTCAGTTTAGTTCCAGTCGAAAAAGGTGCTGCTTCCACTAGCTTTCTGCTTAAACTTTTAAAGGCAGCCAACATTCTCAATGCTTCTTCTTCTTCAAAAATGGAGTTGGCTAGAAGGGTAGGGCTTCAATTGGATGATGCAACAGTCAATGACTTGCTAATCCCCTCTGCGTCGTGCGAAAGCGACACGCGATATGATGTGGACATAGTCATGACAATTTTGGAGCACTTCTTGCAGCAGGGGCAGAGCCCCCCAACTAGTCCTCCGAGGTCCAAGCTTGGTTTCGAAAGGAGGAGGTCTCGCTCTGCGGAGAACATTGATTTTGAGTTTCAGGAGAGCCGGAGATCTTCCTCGGCGTCACATAGCTCGAAGCTGAAGGTGGCCAAGATTATAGATGTATATCTTCAGGAGATTGCCCGGGATGTCAATCTGTCCCTCTCAAAATTCATTGCAATTGCTGAGGCAATACCAGAGTTTGCAAGGCTTGACCATGATGATCTCTACAGAGCAATTGACATTTACCTCAAGGCACATCCGGACCTAAACAAAAGTGAAAGGAAGAGGCTTTGCAGAATTCTAGACTGCAAAAAGCTCTCAATGGAGGTCTGCATGCATGCTGCACAAAATGAGCTGCTCCCACTAAGAGTGGTGGTCCAAGTTCTTTTCTTTGAGCAAGCTAAAGCCGCCATGGCTGGCGGCAAAGTGACCGAGCTGCCTAGCAACATTAGGGCACTGCTAGCCACACATAACATTGATCCATCGAGGCCTCCGCCCTCTATAAGCAACGCTAGTATTGTTCCGGCGGAGGATCAATGGAGCGTTTCAGGCCTGAAATCGCCAAAGTCAAAGTCGTCAACTTTACGAATGAAACTGGATGAAGACGATGATTTGGACGACAATGATATGCATCCTGATGGAATTGGGAGAAATTCTAAGTTCAAATCTCTTTGCTCACTTCCTGCTAAACCCAAAAGAATGTTCAGTAAGTTGTGGTCCATAAACAGAACTAGCAGTGAAAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

633

Amino Acids

70.62

Weight (kDa)

8.59

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 212 - 494 3.9e-93 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
Acc16I TGCGCA 1 cut(s) 253
Acc36I ACCTGC 1 cut(s) 336
AccII CGCG 1 cut(s) 1063
AccIII TCCGGA 1 cut(s) 1408
AciI CCGC 7 cut(s) 318, 752, 1181, 1557, 1569, 1643, 1676
AclWI GGATC 4 cut(s) 15, 28, 1622, 1689
AcoI YGGCCR 2 cut(s) 26, 1254
AcsI RAATTY 5 cut(s) 711, 744, 1316, 1446, 1801
AcuI CTGAAG 4 cut(s) 93, 416, 1262, 1268
AcyI GRCGYC 2 cut(s) 345, 1229
AfaI GTAC 2 cut(s) 179, 326
AgsI TTSAA 9 cut(s) 81, 98, 192, 388, 494, 742, 969, 1001, 1813
AhdI GACNNNNNGTC 1 cut(s) 577
AhlI ACTAGT 1 cut(s) 1130
AjnI CCWGG 1 cut(s) 554
AjuI GAANNNNNNNTTGG 4 cut(s) 1139, 1171, 1835, 1867
Alw21I GWGCWC 3 cut(s) 249, 440, 1101
Alw26I GTCTC 3 cut(s) 70, 728, 1176
AlwI GGATC 4 cut(s) 15, 28, 1622, 1689
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 1 cut(s) 1290
Aor13HI TCCGGA 1 cut(s) 1408
AoxI GGCC 5 cut(s) 26, 617, 1254, 1637, 1699
ApeKI GCWGC 8 cut(s) 157, 803, 902, 938, 1108, 1490, 1504, 1585
ApoI RAATTY 5 cut(s) 711, 744, 1316, 1446, 1801
ArsI GACNNNNNNTTYG 2 cut(s) 1706, 1738
AspLEI GCGC 2 cut(s) 254, 290
AspS9I GGNCC 4 cut(s) 1143, 1411, 1525, 1866
AsuC2I CCSGG 3 cut(s) 25, 1291, 1292
AsuII TTCGAA 1 cut(s) 1158
AsuNHI GCTAGC 1 cut(s) 1609
AvaI CYCGRG 1 cut(s) 1290
AvaII GGWCC 4 cut(s) 1143, 1411, 1525, 1866
BaeGI GKGCMC 1 cut(s) 1606
BalI TGGCCA 1 cut(s) 1256
BamHI GGATCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 249, 1124
BbsI GAAGAC 1 cut(s) 1758
Bbv12I GWGCWC 3 cut(s) 249, 440, 1101
BbvCI CCTCAGC 2 cut(s) 642, 1332
BbvI GCAGC 8 cut(s) 144, 815, 889, 950, 1120, 1477, 1491, 1572
BccI CCATC 5 cut(s) 627, 753, 878, 1639, 1781
BceAI ACGGC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 881, 915
BciT130I CCWGG 1 cut(s) 556
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 3 cut(s) 25, 1291, 1292
BcoDI GTCTC 3 cut(s) 70, 728, 1176
BcuI ACTAGT 1 cut(s) 1130
BfaI CTAG 9 cut(s) 720, 912, 984, 1131, 1451, 1590, 1610, 1662, 1881
BfmI CTRYAG 4 cut(s) 158, 329, 621, 1375
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 336
BfuI GTATCC 1 cut(s) 25
BglI GCCNNNNNGGC 1 cut(s) 1562
BglII AGATCT 1 cut(s) 1217
Bme1390I CCNGG 4 cut(s) 25, 556, 1291, 1292
Bme18I GGWCC 4 cut(s) 1143, 1411, 1525, 1866
BmeRI GACNNNNNGTC 1 cut(s) 577
BmeT110I CYCGRG 1 cut(s) 1290
BmgT120I GGNCC 4 cut(s) 1143, 1411, 1525, 1866
BmiI GGNNCC 2 cut(s) 22, 592
BmrFI CCNGG 4 cut(s) 25, 556, 1291, 1292
BmrI ACTGGG 1 cut(s) 219
BmsI GCATC 5 cut(s) 279, 405, 724, 1000, 1789
BmtI GCTAGC 1 cut(s) 1613
BmuI ACTGGG 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 1758
BplI GAGNNNNNCTC 2 cut(s) 1156, 1188
BpmI CTGGAG 1 cut(s) 395
Bpu10I CCTNAGC 2 cut(s) 642, 1332
Bpu14I TTCGAA 1 cut(s) 1158
BpuEI CTTGAG 1 cut(s) 1382
BpuMI CCSGG 3 cut(s) 25, 1291, 1292
BsaBI GATNNNNATC 3 cut(s) 1272, 1299, 1779
BsaHI GRCGYC 2 cut(s) 345, 1229
BsaI GGTCTC 1 cut(s) 1176
BsaJI CCNNGG 6 cut(s) 339, 403, 1139, 1224, 1290, 1560
BsaWI WCCGGW 1 cut(s) 1408
BsaXI ACNNNNNCTCC 2 cut(s) 1120, 1150
Bse1I ACTGG 4 cut(s) 225, 378, 887, 1749
Bse3DI GCAATG 3 cut(s) 592, 648, 1320
Bse8I GATNNNNATC 3 cut(s) 1272, 1299, 1779
BseAI TCCGGA 1 cut(s) 1408
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 6 cut(s) 339, 403, 1139, 1224, 1290, 1560
BseGI GGATG 7 cut(s) 396, 638, 1012, 1300, 1405, 1753, 1780
BseJI GATNNNNATC 3 cut(s) 1272, 1299, 1779
BseMI GCAATG 3 cut(s) 592, 648, 1320
BseMII CTCAG 6 cut(s) 39, 165, 298, 312, 633, 1323
BseNI ACTGG 4 cut(s) 225, 378, 887, 1749
BseRI GAGGAG 3 cut(s) 58, 479, 1180
BseSI GKGCMC 1 cut(s) 1606
BseX3I CGGCCG 1 cut(s) 26
BseXI GCAGC 8 cut(s) 144, 815, 889, 950, 1120, 1477, 1491, 1572
BsgI GTGCAG 1 cut(s) 1476
Bsh1236I CGCG 1 cut(s) 1063
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 5 cut(s) 28, 619, 1256, 1639, 1701
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 3 cut(s) 249, 440, 1101
BsiHKCI CYCGRG 1 cut(s) 1290
BsiSI CCGG 7 cut(s) 25, 29, 604, 1213, 1291, 1409, 1673
BslFI GGGAC 2 cut(s) 428, 1291
BsmAI GTCTC 3 cut(s) 70, 728, 1176
BsmFI GGGAC 2 cut(s) 428, 1291
BsnI GGCC 5 cut(s) 28, 619, 1256, 1639, 1701
Bso31I GGTCTC 1 cut(s) 1176
BsoBI CYCGRG 1 cut(s) 1290
Bsp119I TTCGAA 1 cut(s) 1158
Bsp1286I GDGCHC 5 cut(s) 249, 440, 1101, 1124, 1606
Bsp13I TCCGGA 1 cut(s) 1408
Bsp143I GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
Bsp19I CCATGG 1 cut(s) 1560
BspACI CCGC 7 cut(s) 318, 752, 1181, 1557, 1569, 1643, 1676
BspANI GGCC 5 cut(s) 28, 619, 1256, 1639, 1701
BspCNI CTCAG 6 cut(s) 40, 166, 297, 313, 634, 1324
BspEI TCCGGA 1 cut(s) 1408
BspFNI CGCG 1 cut(s) 1063
BspHI TCATGA 1 cut(s) 1083
BspLI GGNNCC 2 cut(s) 22, 592
BspMAI CTGCAG 3 cut(s) 162, 333, 625
BspMI ACCTGC 1 cut(s) 336
BspOI GCTAGC 1 cut(s) 1613
BspPI GGATC 4 cut(s) 15, 28, 1622, 1689
BspT104I TTCGAA 1 cut(s) 1158
BspTNI GGTCTC 1 cut(s) 1176
BsrDI GCAATG 3 cut(s) 592, 648, 1320
BsrI ACTGG 4 cut(s) 225, 378, 887, 1749
BssECI CCNNGG 6 cut(s) 339, 403, 1139, 1224, 1290, 1560
BssMI GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
BssNI GRCGYC 2 cut(s) 345, 1229
BssT1I CCWWGG 1 cut(s) 1560
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 2 cut(s) 407, 1018
Bst6I CTCTTC 1 cut(s) 1427
BstACI GRCGYC 2 cut(s) 345, 1229
BstAPI GCANNNNNTGC 1 cut(s) 1105
BstBI TTCGAA 1 cut(s) 1158
BstC8I GCNNGC 7 cut(s) 621, 625, 1484, 1488, 1548, 1567, 1611
BstDEI CTNAG 8 cut(s) 48, 174, 284, 321, 642, 1332, 1514, 1806
BstDSI CCRYGG 2 cut(s) 403, 1560
BstF5I GGATG 7 cut(s) 396, 638, 1012, 1300, 1405, 1753, 1780
BstFNI CGCG 1 cut(s) 1063
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 254, 290
BstKTI GATC 8 cut(s) 23, 88, 793, 861, 1220, 1372, 1630, 1684
BstMAI GTCTC 3 cut(s) 70, 728, 1176
BstMBI GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1556, 1562, 1610, 1698
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 2 cut(s) 1486, 1490
BstSCI CCNGG 4 cut(s) 23, 554, 1289, 1290
BstSFI CTRYAG 4 cut(s) 158, 329, 621, 1375
BstSLI GKGCMC 1 cut(s) 1606
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 8 cut(s) 144, 815, 889, 950, 1120, 1477, 1491, 1572
BstV2I GAAGAC 1 cut(s) 1758
BstX2I RGATCY 2 cut(s) 20, 1217
BstYI RGATCY 2 cut(s) 20, 1217
BstZI CGGCCG 1 cut(s) 26
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 5 cut(s) 28, 619, 1256, 1639, 1701
BtgI CCRYGG 2 cut(s) 403, 1560
BtsCI GGATG 7 cut(s) 396, 638, 1012, 1300, 1405, 1753, 1780
BtsI GCAGTG 2 cut(s) 1604, 1891
BtsIMutI CAGTG 2 cut(s) 1604, 1891
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 7 cut(s) 621, 625, 1484, 1488, 1548, 1567, 1611
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 1083
CfoI GCGC 2 cut(s) 254, 290
Cfr13I GGNCC 4 cut(s) 1143, 1411, 1525, 1866
Cfr9I CCCGGG 1 cut(s) 1290
CseI GACGC 4 cut(s) 220, 795, 1032, 1218
Csp6I GTAC 2 cut(s) 178, 325
CviAII CATG 6 cut(s) 215, 1084, 1364, 1483, 1487, 1561
CviQI GTAC 2 cut(s) 178, 325
DdeI CTNAG 8 cut(s) 48, 174, 284, 321, 642, 1332, 1514, 1806
DpnI GATC 8 cut(s) 22, 87, 792, 860, 1219, 1371, 1629, 1683
DpnII GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
DraI TTTAAA 1 cut(s) 933
DriI GACNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 2 cut(s) 26, 1254
EagI CGGCCG 1 cut(s) 26
Eam1104I CTCTTC 1 cut(s) 1427
Eam1105I GACNNNNNGTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 1427
EciI GGCGGA 2 cut(s) 1632, 1691
Ecl136II GAGCTC 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 1560
Eco147I AGGCCT 3 cut(s) 619, 1639, 1701
Eco24I GRGCYC 2 cut(s) 249, 1124
Eco31I GGTCTC 1 cut(s) 1176
Eco47I GGWCC 4 cut(s) 1143, 1411, 1525, 1866
Eco52I CGGCCG 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 4 cut(s) 93, 416, 1262, 1268
Eco88I CYCGRG 1 cut(s) 1290
EcoICRI GAGCTC 1 cut(s) 247
EcoRI GAATTC 1 cut(s) 1446
EcoRII CCWGG 1 cut(s) 554
EcoT14I CCWWGG 1 cut(s) 1560
EcoT22I ATGCAT 3 cut(s) 739, 1488, 1782
EcoT38I GRGCYC 2 cut(s) 249, 1124
ErhI CCWWGG 1 cut(s) 1560
FaeI CATG 6 cut(s) 218, 1087, 1367, 1486, 1490, 1564
FaqI GGGAC 2 cut(s) 428, 1291
FatI CATG 6 cut(s) 214, 1083, 1363, 1482, 1486, 1560
FbaI TGATCA 1 cut(s) 790
FokI GGATG 7 cut(s) 383, 645, 1019, 1307, 1392, 1760, 1767
FriOI GRGCYC 2 cut(s) 249, 1124
FspBI CTAG 9 cut(s) 720, 912, 984, 1131, 1451, 1590, 1610, 1662, 1881
FspI TGCGCA 1 cut(s) 253
GlaI GCGC 2 cut(s) 253, 289
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 5 cut(s) 28, 619, 1256, 1639, 1701
HapII CCGG 7 cut(s) 25, 29, 604, 1213, 1291, 1409, 1673
HgaI GACGC 4 cut(s) 220, 795, 1032, 1218
HhaI GCGC 2 cut(s) 254, 290
Hin1I GRCGYC 2 cut(s) 345, 1229
Hin1II CATG 6 cut(s) 218, 1087, 1367, 1486, 1490, 1564
Hin6I GCGC 2 cut(s) 252, 288
HinP1I GCGC 2 cut(s) 252, 288
HincII GTYRAC 1 cut(s) 1728
HindII GTYRAC 1 cut(s) 1728
HindIII AAGCTT 1 cut(s) 1148
HinfI GANTC 7 cut(s) 185, 416, 474, 812, 818, 824, 866
HpaII CCGG 7 cut(s) 25, 29, 604, 1213, 1291, 1409, 1673
Hpy166II GTNNAC 2 cut(s) 1075, 1728
Hpy188I TCNGA 9 cut(s) 73, 175, 435, 463, 595, 811, 817, 823, 1140
Hpy188III TCNNGA 7 cut(s) 349, 1084, 1205, 1280, 1409, 1451, 1784
Hpy8I GTNNAC 2 cut(s) 1075, 1728
Hpy99I CGWCG 4 cut(s) 236, 350, 811, 1048
HpyAV CCTTC 4 cut(s) 346, 534, 981, 1243
HpyCH4III ACNGT 2 cut(s) 407, 1018
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1556, 1562, 1610, 1698
HpyF3I CTNAG 8 cut(s) 48, 174, 284, 321, 642, 1332, 1514, 1806
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 2 cut(s) 345, 1229
Hsp92II CATG 6 cut(s) 218, 1087, 1367, 1486, 1490, 1564
HspAI GCGC 2 cut(s) 252, 288
Kpn2I TCCGGA 1 cut(s) 1408
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
LmnI GCTCC 6 cut(s) 216, 466, 596, 1096, 1512, 1689
Lsp1109I GCAGC 8 cut(s) 144, 815, 889, 950, 1120, 1477, 1491, 1572
LweI GCATC 5 cut(s) 279, 405, 724, 1000, 1789
MaeI CTAG 9 cut(s) 720, 912, 984, 1131, 1451, 1590, 1610, 1662, 1881
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 1230, 1576
MalI GATC 8 cut(s) 22, 87, 792, 860, 1219, 1371, 1629, 1683
MboI GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
MboII GAAGA 9 cut(s) 180, 376, 951, 954, 957, 1212, 1268, 1444, 1763
MfeI CAATTG 5 cut(s) 420, 498, 1001, 1326, 1383
MflI RGATCY 2 cut(s) 20, 1217
MhlI GDGCHC 5 cut(s) 249, 440, 1101, 1124, 1606
MlsI TGGCCA 1 cut(s) 1256
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 3 cut(s) 410, 818, 875
MmeI TCCRAC 3 cut(s) 389, 441, 789
Mox20I TGGCCA 1 cut(s) 1256
Mph1103I ATGCAT 3 cut(s) 739, 1488, 1782
MroI TCCGGA 1 cut(s) 1408
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 3 cut(s) 839, 924, 932
MslI CAYNNNNRTG 3 cut(s) 484, 1082, 1785
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 7 cut(s) 25, 29, 604, 1213, 1291, 1409, 1673
MspR9I CCNGG 4 cut(s) 25, 556, 1291, 1292
MunI CAATTG 5 cut(s) 420, 498, 1001, 1326, 1383
MvaI CCWGG 1 cut(s) 556
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 8 cut(s) 163, 597, 1105, 1114, 1556, 1562, 1610, 1698
NciI CCSGG 3 cut(s) 25, 1291, 1292
NcoI CCATGG 1 cut(s) 1560
NdeII GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
NheI GCTAGC 1 cut(s) 1609
NlaIII CATG 6 cut(s) 218, 1087, 1367, 1486, 1490, 1564
NlaIV GGNNCC 2 cut(s) 22, 592
NmeAIII GCCGAG 1 cut(s) 1205
NmuCI GTSAC 2 cut(s) 1230, 1576
NsbI TGCGCA 1 cut(s) 253
NsiI ATGCAT 3 cut(s) 739, 1488, 1782
NspI RCATGY 2 cut(s) 1486, 1490
NspV TTCGAA 1 cut(s) 1158
PaeI GCATGC 2 cut(s) 1486, 1490
PagI TCATGA 1 cut(s) 1083
PceI AGGCCT 3 cut(s) 619, 1639, 1701
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PfeI GAWTC 4 cut(s) 185, 474, 812, 818
PflFI GACNNNGTC 1 cut(s) 1079
PfoI TCCNGGA 1 cut(s) 554
PleI GAGTC 3 cut(s) 410, 818, 874
PpsI GAGTC 3 cut(s) 410, 818, 874
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 554
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 22, 592
PspPI GGNCC 4 cut(s) 1143, 1411, 1525, 1866
PstI CTGCAG 3 cut(s) 162, 333, 625
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 20, 1217
PsyI GACNNNGTC 1 cut(s) 1079
RsaI GTAC 2 cut(s) 179, 326
RsaNI GTAC 2 cut(s) 178, 325
RseI CAYNNNNRTG 3 cut(s) 484, 1082, 1785
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 839, 924, 932
Sau3AI GATC 8 cut(s) 20, 85, 790, 858, 1217, 1369, 1627, 1681
Sau96I GGNCC 4 cut(s) 1143, 1411, 1525, 1866
SbfI CCTGCAGG 1 cut(s) 625
SchI GAGTC 3 cut(s) 410, 818, 875
ScrFI CCNGG 4 cut(s) 25, 556, 1291, 1292
SdaI CCTGCAGG 1 cut(s) 625
SduI GDGCHC 5 cut(s) 249, 440, 1101, 1124, 1606
SfaNI GCATC 5 cut(s) 279, 405, 724, 1000, 1789
SfcI CTRYAG 4 cut(s) 158, 329, 621, 1375
SfuI TTCGAA 1 cut(s) 1158
SinI GGWCC 4 cut(s) 1143, 1411, 1525, 1866
SmaI CCCGGG 1 cut(s) 1292
SmiMI CAYNNNNRTG 3 cut(s) 484, 1082, 1785
SmlI CTYRAG 1 cut(s) 1397
SmoI CTYRAG 1 cut(s) 1397
SpeI ACTAGT 1 cut(s) 1130
SphI GCATGC 2 cut(s) 1486, 1490
Sse8387I CCTGCAGG 1 cut(s) 625
SseBI AGGCCT 3 cut(s) 619, 1639, 1701
SsiI CCGC 7 cut(s) 318, 752, 1181, 1557, 1569, 1643, 1676
SspMI CTAG 9 cut(s) 720, 912, 984, 1131, 1451, 1590, 1610, 1662, 1881
SstI GAGCTC 1 cut(s) 249
StuI AGGCCT 3 cut(s) 619, 1639, 1701
StyD4I CCNGG 4 cut(s) 23, 554, 1289, 1290
StyI CCWWGG 1 cut(s) 1560
TaaI ACNGT 2 cut(s) 407, 1018
TaiI ACGT 1 cut(s) 348
TaqII GACCGA 2 cut(s) 353, 1595
TauI GCSGC 4 cut(s) 320, 755, 1559, 1572
TfiI GAWTC 4 cut(s) 185, 474, 812, 818
Tru1I TTAA 3 cut(s) 839, 924, 932
Tru9I TTAA 3 cut(s) 839, 924, 932
TscAI CASTG 2 cut(s) 1611, 1891
TseFI GTSAC 2 cut(s) 1230, 1576
TseI GCWGC 8 cut(s) 157, 803, 902, 938, 1108, 1490, 1504, 1585
Tsp45I GTSAC 2 cut(s) 1230, 1576
TspDTI ATGAA 5 cut(s) 17, 26, 1309, 1754, 1764
TspMI CCCGGG 1 cut(s) 1290
TspRI CASTG 2 cut(s) 1611, 1891
Tth111I GACNNNGTC 1 cut(s) 1079
VpaK11BI GGWCC 4 cut(s) 1143, 1411, 1525, 1866
XapI RAATTY 5 cut(s) 711, 744, 1316, 1446, 1801
XbaI TCTAGA 1 cut(s) 1450
XceI RCATGY 2 cut(s) 1486, 1490
XmaI CCCGGG 1 cut(s) 1290
XspI CTAG 9 cut(s) 720, 912, 984, 1131, 1451, 1590, 1610, 1662, 1881
ZraI GACGTC 1 cut(s) 346
Zsp2I ATGCAT 3 cut(s) 739, 1488, 1782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.