Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008808

Physical Location & Seq

Genomic Coordinates Reverse (-)

12188221 .. 12199318

Gene Structure 11098 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1156500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGGGCGCGCCAGATAAAGCGCCGGTCAATTCGAATTCGATGCAGCGTGTTAAGGTTTACCGTCTGAGTGAGGATGGAAAATGGGATGATCAGGGAACTGGTCATGTCACGGTTGACTATATGGAGCGTTCAGAAGAGCTGGGTTTGTTTGTTATTGATGAAGAAGACAATGAGACATTACTTCTGCATCGAATCAGCTCGGATGACATTTACAGAAAGCAAGAAGATACAATTATCTCATGGAGGGATCCAGAATATTCTACTGAATTAGCTCTCAGTTTTCAGGAGCCTACAGGCTGCTCTTACATATGGGACCACATATGCAGCGTTCAAAGGAGTATACATTTTAATTCTCTTAACAATGAGACATTTCACAGTGCAAACAGTGAGTTGAGGGAGCTCCCTACGGTTGAGCTATCCACACTTCCTTTAATCCTTAAGACTGTGATCGAGAGTGGCATTGCTGATCAGATGCGACTTACAGAATTGATATTGACTGATCAAGAGTTTTTTCGAAAGCTCATGGGCCTATTTAGAATCTGTGAAGACTTAAAAAATGTTGATGGTCTTCACATGATATACAAAATAGTAAGGGGGATCATTCTGCTGAATAGTCCTCAGATCTTTGAGAAAATATTCGGTGATGATTTAATCATGGATATCATCGGTTCCCTTGAGTATGACCCTGAAGTACCTAATGTCCAACACCATCGTAACTTTTTGAAAGAACATGTTGTTTTTAAGGAGGCCATACCGATTAAAGATGCTATTGTGTTGTCAAAGATACACCAGACGTATAAAGTTGGATATTTAAAGGATGTTGTTTTGGCTAGAGTTCTGGATGAGGCCACAGTTGCAAATCTCAATTCCATTATTCATGCAAACAACGCTATTGTTGTTTCTTTGCTAAAGGATGACAGTACCTTTATTCAAGAATTGTTTGCTAGATTACGGTCGCCATCCACCTCTGCAGAATCTAAAAAGAATTTGGTATATTTCTTGCACGAGTTTTGTAGTTTAAGCAAAAGCCTGCAGATGGTCCAGCAGCTTCGGCTATTTAGGGATCTCATGAATGAAGGAATCTTTGACATTGTAACTGATGTGTTGCAGAGTGAAGACAAAAAGCTTGTATTAACTGGGACAGATATCCTGAGTCTTTTCATGAATCAGGATCCAAACCTTCTGCGCTCATATGTTGTTCGTCAGGAAGGAACTCCACTTTTTGGACTCTTGGTTAAAGGAATGATTACAGATTTCGGAGAGGACATGCACTGCCAGTTTCTTGAAATTCTTCGTAGTCTACTAGATTCATACACATTGTCAGGAGCTCAGAGAGATGCAATTATCGAGATATTCTATGAGAAGCATCTGGGGCAGTTAATTGATGTTATAACAGCGTCATGTTCTTTGGAAGGCAATGGTCAATCATTTGATAAATCTTCTATCTCTGGTGGAAGAGTTGAAAGTCAGAGTGTTGCGAAGCCTGAAATATTATCAAATATATGCGAATTGCTTTGTTTTTGTGTCCTTCACCATCCATTCAGAATAAAGTGTAACTTTCTGCTTAGTAATGTGATGGATAAAGTTTTGTTGGTTACACAAAGAAGAGAAAAGTATTTAGTTGTTGCTGCAGTTCGATTTGTTCGCACTATTCTTTCACGTCATGATGAGCATCTGATAAATCATATTGTTAAGAACAACCTTCTGAAGCCAATTGTCGATGCATTTGTTGGTAATGGAAATCGATATAACCTGCTGAACTCGGCTATTTTAGAACTTTTTGAGTTCATCCGGAAGGAAAACTTGAAGTCTTTGGTTAAATACTTGGTTGATTCATTCTGGGATCAGTTGGTCAGATTTGAGAATTTTGCATCGGTTATCTCCCTCAAAGTAAAATATGAGCAGTGCCTAGAACATTTTGGAACCAAAGGCACCGCTAATGTGTCAGACCCTAGAAAACGAAACGATGAGCGTGCTTTGGAGAAAGAAGAAGAAGAGTATTTCAACGAGGACAGTGATGAAGAAGATACAGCATCTGCATCCATGCCAAATACCCAAAAAGTACAGACCCAACCGCAACCTGTTTTATCCAATGGAGTTGCTGCAAGTTATCCACCATCCAGTCCAAGGTCTGTCGGGCTGGTGGATTATGATGACGATGAGGACGACGAAGACTACAAGCCACCGCCCAAGAAACATCCTGAACCTTCTGATGAAGATGAAGGAACAATGGAGTCCCTTAGGTTGAAGCGGAAATTGGCTTCTAAGGATAGGGAGACCGAGCTAGCAAAGAGGCAACGGTTGGGTAAAAACTCCAAGCCAAAAGAAAGTGTGTTTGCTGCTTTGTGTACAACTCTAAGCCAGGCAGTGCTACCGAATAAGAAAATAACTCGCACAGCTGAGGGGGTCAACTCAGGTGAAGGGAAAAATCAAGAGAATGATGAGCATGCTGTTTCTAAGAGCTGTTCTGAGAACAACAGTTGCGATGATGCTGACAATAGGGAAAATGAGCCTACTACTTCTAGAGGCTGCTCGGATCACTTGCATGGCACTCCGGATGATAGACAGTTGGGTGGAGAGGACTGCCCGTTGATACCACCAAAATCTTCACCGGAAATGGCTGTAAATGGGTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04212 ko04922 map04212 map04922

Pfam Domains

Protein Families

SMK-1

Protein Analysis

879

Amino Acids

99.42

Weight (kDa)

5.1

Isoelectric Point (pI)

38.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EVH1_PP4R3	PF22972	15 - 113	8.7e-17	EVH1-like domain in PP4R3 protein
PP4R3	PF04802	152 - 635	2.7e-154	Phosphatase 4 regulatory subunit 3

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06670 AT3G06670
fragaria_vesca	FvH4_4g19450 FvH4_4g19450 FvH4_4g19450 FvH4_4g19450
malus_domestica	MD13G1156500.v1.1 MD16G1157400.v1.1
prunus_persica	Prupe.1G122300_v2.0.a1 Prupe.1G122300_v2.0.a1
pyrus_communis	pycom13g13390 pycom16g13230
rosa_chinensis	RchiOBHm_Chr4g0424501
rosa_laevigata	RLG00000007468
rosa_multiflora	Rmu_sc0002603.1_g000008 Rmu_sc0003942.1_g000025
rosa_roxburghii	Rroxscaffold_5G00366430
rosa_rugosa	Rorug04G0198000
rosa_samantha	Rh4AG254400 Rh4BG259800 Rh4CG270900 Rh4DG254200
rosa_wichuraiana	Rw4G021970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1391
Acc36I	ACCTGC	1 cut(s)	1761
AccB1I	GGYRCC	1 cut(s)	1931
AccB7I	CCANNNNNTGG	1 cut(s)	1223
AccI	GTMKAC	2 cut(s)	340, 1300
AccII	CGCG	1 cut(s)	8
AccIII	TCCGGA	2 cut(s)	1791, 2554
AciI	CCGC	4 cut(s)	1935, 2075, 2186, 2251
AclWI	GGATC	8 cut(s)	242, 255, 605, 1071, 1166, 1179, 1851, 2544
AcsI	RAATTY	4 cut(s)	34, 985, 1287, 1864
AcuI	CTGAAG	2 cut(s)	708, 1727
AfaI	GTAC	4 cut(s)	693, 922, 2064, 2350
AfiI	CCNNNNNNNGG	3 cut(s)	1036, 1223, 2127
AflII	CTTAAG	1 cut(s)	437
AflIII	ACRYGT	1 cut(s)	730
AgsI	TTSAA	8 cut(s)	332, 724, 932, 1286, 1463, 1807, 2005, 2248
AjiI	CACGTC	1 cut(s)	1661
AjnI	CCWGG	1 cut(s)	2361
AjuI	GAANNNNNNNTTGG	2 cut(s)	2240, 2272
Alw21I	GWGCWC	2 cut(s)	402, 1330
Alw26I	GTCTC	3 cut(s)	167, 359, 2270
AlwI	GGATC	8 cut(s)	242, 255, 605, 1071, 1166, 1179, 1851, 2544
AlwNI	CAGNNNCTG	1 cut(s)	2036
Aor13HI	TCCGGA	2 cut(s)	1791, 2554
AoxI	GGCC	3 cut(s)	526, 747, 846
ApeKI	GCWGC	8 cut(s)	43, 297, 324, 1045, 1628, 2102, 2339, 2529
ApoI	RAATTY	4 cut(s)	34, 985, 1287, 1864
AscI	GGCGCGCC	1 cut(s)	6
Asp700I	GAANNNNTTC	2 cut(s)	635, 1290
AspLEI	GCGC	4 cut(s)	8, 10, 22, 1188
AspS9I	GGNCC	3 cut(s)	313, 526, 1039
AsuHPI	GGTGA	4 cut(s)	653, 1523, 2429, 2601
AsuII	TTCGAA	2 cut(s)	32, 514
AsuNHI	GCTAGC	1 cut(s)	2284
AvaII	GGWCC	2 cut(s)	313, 1039
AxyI	CCTNAGG	1 cut(s)	2240
BamHI	GGATCC	2 cut(s)	247, 1171
BanI	GGYRCC	1 cut(s)	1931
BanII	GRGCYC	2 cut(s)	402, 1330
BauI	CACGAG	1 cut(s)	1004
BbsI	GAAGAC	5 cut(s)	171, 552, 560, 1122, 2178
Bbv12I	GWGCWC	2 cut(s)	402, 1330
BbvCI	CCTCAGC	1 cut(s)	2400
BbvI	GCAGC	8 cut(s)	55, 284, 336, 1057, 1615, 2089, 2326, 2516
BccI	CCATC	8 cut(s)	68, 557, 717, 967, 1030, 1542, 1570, 2125
BcgI	CGANNNNNNTGC	4 cut(s)	22, 56, 1955, 1989
BciT130I	CCWGG	1 cut(s)	2363
BclI	TGATCA	3 cut(s)	88, 466, 499
BcoDI	GTCTC	3 cut(s)	167, 359, 2270
BfaI	CTAG	7 cut(s)	831, 945, 1304, 1910, 1953, 2285, 2523
BfmI	CTRYAG	4 cut(s)	291, 969, 1031, 1629
BfoI	RGCGCY	1 cut(s)	23
BfrI	CTTAAG	1 cut(s)	437
BfuAI	ACCTGC	1 cut(s)	1761
BglII	AGATCT	1 cut(s)	621
BisI	GCNGC	8 cut(s)	44, 298, 325, 1046, 1629, 2103, 2340, 2530
BlsI	GCNGC	8 cut(s)	45, 299, 326, 1047, 1630, 2104, 2341, 2531
Bme1390I	CCNGG	1 cut(s)	2363
Bme18I	GGWCC	2 cut(s)	313, 1039
BmgBI	CACGTC	1 cut(s)	1661
BmgT120I	GGNCC	3 cut(s)	313, 526, 1039
BmiI	GGNNCC	7 cut(s)	249, 288, 314, 670, 1173, 1924, 1933
BmrFI	CCNGG	1 cut(s)	2363
BmrI	ACTGGG	1 cut(s)	1146
BmtI	GCTAGC	1 cut(s)	2288
BmuI	ACTGGG	1 cut(s)	1146
BpiI	GAAGAC	5 cut(s)	171, 552, 560, 1122, 2178
Bpu10I	CCTNAGC	1 cut(s)	2400
Bpu14I	TTCGAA	2 cut(s)	32, 514
BpuEI	CTTGAG	1 cut(s)	695
Bsa29I	ATCGAT	1 cut(s)	1744
BsaBI	GATNNNNATC	1 cut(s)	1671
BsaI	GGTCTC	1 cut(s)	2270
BsaJI	CCNNGG	1 cut(s)	2126
BsaWI	WCCGGW	3 cut(s)	1791, 2554, 2611
Bsc4I	CCNNNNNNNGG	3 cut(s)	1036, 1223, 2127
Bse118I	RCCGGY	1 cut(s)	22
Bse1I	ACTGG	4 cut(s)	103, 1141, 1276, 2121
Bse21I	CCTNAGG	1 cut(s)	2240
Bse3DI	GCAATG	2 cut(s)	459, 1423
Bse8I	GATNNNNATC	1 cut(s)	1671
BseAI	TCCGGA	2 cut(s)	1791, 2554
BseBI	CCWGG	1 cut(s)	2363
BseCI	ATCGAT	1 cut(s)	1744
BseDI	CCNNGG	1 cut(s)	2126
BseJI	GATNNNNATC	1 cut(s)	1671
BseLI	CCNNNNNNNGG	3 cut(s)	1036, 1223, 2127
BseMI	GCAATG	2 cut(s)	459, 1423
BseMII	CTCAG	8 cut(s)	56, 289, 632, 1142, 1343, 2391, 2427, 2460
BseNI	ACTGG	4 cut(s)	103, 1141, 1276, 2121
BsePI	GCGCGC	1 cut(s)	6
BseXI	GCAGC	8 cut(s)	55, 284, 336, 1057, 1615, 2089, 2326, 2516
BseYI	CCCAGC	1 cut(s)	139
Bsh1236I	CGCG	1 cut(s)	8
Bsh1285I	CGRYCG	1 cut(s)	956
BshFI	GGCC	3 cut(s)	528, 749, 848
BshNI	GGYRCC	1 cut(s)	1931
BshVI	ATCGAT	1 cut(s)	1744
BsiEI	CGRYCG	1 cut(s)	956
BsiHKAI	GWGCWC	2 cut(s)	402, 1330
BsiSI	CCGG	4 cut(s)	23, 1792, 2555, 2612
BslFI	GGGAC	3 cut(s)	326, 1153, 2221
BslI	CCNNNNNNNGG	3 cut(s)	1036, 1223, 2127
BsmAI	GTCTC	3 cut(s)	167, 359, 2270
BsmFI	GGGAC	3 cut(s)	326, 1153, 2221
BsnI	GGCC	3 cut(s)	528, 749, 848
Bso31I	GGTCTC	1 cut(s)	2270
Bsp119I	TTCGAA	2 cut(s)	32, 514
Bsp1286I	GDGCHC	2 cut(s)	402, 1330
Bsp13I	TCCGGA	2 cut(s)	1791, 2554
Bsp1407I	TGTACA	1 cut(s)	2348
BspACI	CCGC	4 cut(s)	1935, 2075, 2186, 2251
BspANI	GGCC	3 cut(s)	528, 749, 848
BspCNI	CTCAG	8 cut(s)	57, 288, 631, 1143, 1342, 2392, 2426, 2461
BspDI	ATCGAT	1 cut(s)	1744
BspEI	TCCGGA	2 cut(s)	1791, 2554
BspFNI	CGCG	1 cut(s)	8
BspHI	TCATGA	3 cut(s)	1068, 1161, 1663
BspLI	GGNNCC	7 cut(s)	249, 288, 314, 670, 1173, 1924, 1933
BspMAI	CTGCAG	3 cut(s)	973, 1035, 1633
BspMI	ACCTGC	1 cut(s)	1761
BspOI	GCTAGC	1 cut(s)	2288
BspPI	GGATC	8 cut(s)	242, 255, 605, 1071, 1166, 1179, 1851, 2544
BspQI	GCTCTTC	1 cut(s)	129
BspT104I	TTCGAA	2 cut(s)	32, 514
BspT107I	GGYRCC	1 cut(s)	1931
BspTI	CTTAAG	1 cut(s)	437
BspTNI	GGTCTC	1 cut(s)	2270
BsrDI	GCAATG	2 cut(s)	459, 1423
BsrFI	RCCGGY	1 cut(s)	22
BsrGI	TGTACA	1 cut(s)	2348
BsrI	ACTGG	4 cut(s)	103, 1141, 1276, 2121
BssAI	RCCGGY	1 cut(s)	22
BssECI	CCNNGG	1 cut(s)	2126
BssHII	GCGCGC	1 cut(s)	6
BssNAI	GTATAC	1 cut(s)	341
BssSI	CACGAG	1 cut(s)	1004
BssT1I	CCWWGG	1 cut(s)	2126
Bst1107I	GTATAC	1 cut(s)	341
Bst2BI	CACGAG	1 cut(s)	1004
Bst2UI	CCWGG	1 cut(s)	2363
Bst6I	CTCTTC	4 cut(s)	129, 1450, 1600, 1989
BstAFI	CTTAAG	1 cut(s)	437
BstAUI	TGTACA	1 cut(s)	2348
BstBI	TTCGAA	2 cut(s)	32, 514
BstC8I	GCNNGC	5 cut(s)	8, 1031, 1974, 2286, 2448
BstFNI	CGCG	1 cut(s)	8
BstH2I	RGCGCY	1 cut(s)	23
BstHHI	GCGC	4 cut(s)	8, 10, 22, 1188
BstMAI	GTCTC	3 cut(s)	167, 359, 2270
BstMCI	CGRYCG	1 cut(s)	956
BstMWI	GCNNNNNNNGC	4 cut(s)	854, 887, 1051, 1372
BstNI	CCWGG	1 cut(s)	2363
BstNSI	RCATGY	3 cut(s)	734, 1270, 2450
BstSCI	CCNGG	1 cut(s)	2361
BstSFI	CTRYAG	4 cut(s)	291, 969, 1031, 1629
BstUI	CGCG	1 cut(s)	8
BstV1I	GCAGC	8 cut(s)	55, 284, 336, 1057, 1615, 2089, 2326, 2516
BstV2I	GAAGAC	5 cut(s)	171, 552, 560, 1122, 2178
BstX2I	RGATCY	4 cut(s)	247, 621, 1063, 1171
BstYI	RGATCY	4 cut(s)	247, 621, 1063, 1171
BstZ17I	GTATAC	1 cut(s)	341
Bsu15I	ATCGAT	1 cut(s)	1744
Bsu36I	CCTNAGG	1 cut(s)	2240
BsuRI	GGCC	3 cut(s)	528, 749, 848
BsuTUI	ATCGAT	1 cut(s)	1744
BtgZI	GCGATG	1 cut(s)	2499
BtrI	CACGTC	1 cut(s)	1661
BtsI	GCAGTG	3 cut(s)	1270, 1910, 2373
BtsIMutI	CAGTG	6 cut(s)	382, 391, 1270, 1910, 2020, 2373
BveI	ACCTGC	1 cut(s)	1761
Cac8I	GCNNGC	5 cut(s)	8, 1031, 1974, 2286, 2448
CaiI	CAGNNNCTG	1 cut(s)	2036
CciI	TCATGA	3 cut(s)	1068, 1161, 1663
CfoI	GCGC	4 cut(s)	8, 10, 22, 1188
Cfr10I	RCCGGY	1 cut(s)	22
Cfr13I	GGNCC	3 cut(s)	313, 526, 1039
ClaI	ATCGAT	1 cut(s)	1744
CseI	GACGC	1 cut(s)	1386
Csp6I	GTAC	4 cut(s)	692, 921, 2063, 2349
CviQI	GTAC	4 cut(s)	692, 921, 2063, 2349
DraI	TTTAAA	1 cut(s)	813
Eam1104I	CTCTTC	4 cut(s)	129, 1450, 1600, 1989
EarI	CTCTTC	4 cut(s)	129, 1450, 1600, 1989
Ecl136II	GAGCTC	2 cut(s)	400, 1328
Eco130I	CCWWGG	1 cut(s)	2126
Eco24I	GRGCYC	2 cut(s)	402, 1330
Eco31I	GGTCTC	1 cut(s)	2270
Eco32I	GATATC	2 cut(s)	661, 1147
Eco47I	GGWCC	2 cut(s)	313, 1039
Eco53kI	GAGCTC	2 cut(s)	400, 1328
Eco57I	CTGAAG	2 cut(s)	708, 1727
Eco81I	CCTNAGG	1 cut(s)	2240
EcoICRI	GAGCTC	2 cut(s)	400, 1328
EcoRI	GAATTC	1 cut(s)	34
EcoRII	CCWGG	1 cut(s)	2361
EcoRV	GATATC	2 cut(s)	661, 1147
EcoT14I	CCWWGG	1 cut(s)	2126
EcoT22I	ATGCAT	1 cut(s)	1726
EcoT38I	GRGCYC	2 cut(s)	402, 1330
ErhI	CCWWGG	1 cut(s)	2126
FalI	AAGNNNNNCTT	4 cut(s)	1541, 1573, 1787, 1819
FaqI	GGGAC	3 cut(s)	326, 1153, 2221
FauNDI	CATATG	3 cut(s)	308, 320, 1192
FbaI	TGATCA	3 cut(s)	88, 466, 499
FblI	GTMKAC	2 cut(s)	340, 1300
Fnu4HI	GCNGC	8 cut(s)	44, 298, 325, 1046, 1629, 2103, 2340, 2530
FriOI	GRGCYC	2 cut(s)	402, 1330
Fsp4HI	GCNGC	8 cut(s)	44, 298, 325, 1046, 1629, 2103, 2340, 2530
FspBI	CTAG	7 cut(s)	831, 945, 1304, 1910, 1953, 2285, 2523
GlaI	GCGC	4 cut(s)	7, 9, 21, 1187
GluI	GCNGC	8 cut(s)	44, 298, 325, 1046, 1629, 2103, 2340, 2530
GsaI	CCCAGC	1 cut(s)	143
HaeII	RGCGCY	1 cut(s)	23
HaeIII	GGCC	3 cut(s)	528, 749, 848
HapII	CCGG	4 cut(s)	23, 1792, 2555, 2612
HgaI	GACGC	1 cut(s)	1386
HhaI	GCGC	4 cut(s)	8, 10, 22, 1188
Hin6I	GCGC	4 cut(s)	6, 8, 20, 1186
HinP1I	GCGC	4 cut(s)	6, 8, 20, 1186
HincII	GTYRAC	2 cut(s)	115, 2410
HindII	GTYRAC	2 cut(s)	115, 2410
HindIII	AAGCTT	1 cut(s)	1124
HpaII	CCGG	4 cut(s)	23, 1792, 2555, 2612
HphI	GGTGA	4 cut(s)	653, 1523, 2429, 2601
Hpy166II	GTNNAC	6 cut(s)	58, 115, 341, 1301, 2349, 2410
Hpy8I	GTNNAC	6 cut(s)	58, 115, 341, 1301, 2349, 2410
Hpy99I	CGWCG	1 cut(s)	2171
HpyCH4IV	ACGT	2 cut(s)	794, 1660
HpyF10VI	GCNNNNNNNGC	4 cut(s)	854, 887, 1051, 1372
HpySE526I	ACGT	2 cut(s)	794, 1660
HspAI	GCGC	4 cut(s)	6, 8, 20, 1186
Kpn2I	TCCGGA	2 cut(s)	1791, 2554
Ksp22I	TGATCA	3 cut(s)	88, 466, 499
LguI	GCTCTTC	1 cut(s)	129
LmnI	GCTCC	5 cut(s)	124, 286, 397, 405, 1325
Lsp1109I	GCAGC	8 cut(s)	55, 284, 336, 1057, 1615, 2089, 2326, 2516
MaeI	CTAG	7 cut(s)	831, 945, 1304, 1910, 1953, 2285, 2523
MaeII	ACGT	2 cut(s)	794, 1660
MaeIII	GTNAC	5 cut(s)	106, 713, 1093, 1553, 1594
MfeI	CAATTG	1 cut(s)	1713
MflI	RGATCY	4 cut(s)	247, 621, 1063, 1171
MhlI	GDGCHC	2 cut(s)	402, 1330
MlyI	GAGTC	3 cut(s)	1162, 1221, 2243
MmeI	TCCRAC	2 cut(s)	727, 784
Mph1103I	ATGCAT	1 cut(s)	1726
MroI	TCCGGA	2 cut(s)	1791, 2554
MroXI	GAANNNNTTC	2 cut(s)	635, 1290
MslI	CAYNNNNRTG	1 cut(s)	2544
MspA1I	CMGCKG	1 cut(s)	2399
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	4 cut(s)	23, 1792, 2555, 2612
MspR9I	CCNGG	1 cut(s)	2363
MunI	CAATTG	1 cut(s)	1713
MvaI	CCWGG	1 cut(s)	2363
MvnI	CGCG	1 cut(s)	8
MwoI	GCNNNNNNNGC	4 cut(s)	854, 887, 1051, 1372
NdeI	CATATG	3 cut(s)	308, 320, 1192
NheI	GCTAGC	1 cut(s)	2284
NlaIV	GGNNCC	7 cut(s)	249, 288, 314, 670, 1173, 1924, 1933
NmeAIII	GCCGAG	1 cut(s)	1742
NmuCI	GTSAC	1 cut(s)	106
NsiI	ATGCAT	1 cut(s)	1726
NspI	RCATGY	3 cut(s)	734, 1270, 2450
NspV	TTCGAA	2 cut(s)	32, 514
PaeI	GCATGC	1 cut(s)	2450
PagI	TCATGA	3 cut(s)	1068, 1161, 1663
PalAI	GGCGCGCC	1 cut(s)	6
PauI	GCGCGC	1 cut(s)	6
PciI	ACATGT	1 cut(s)	730
PciSI	GCTCTTC	1 cut(s)	129
PcsI	WCGNNNNNNNCGW	1 cut(s)	2163
PdmI	GAANNNNTTC	2 cut(s)	635, 1290
PfeI	GAWTC	7 cut(s)	192, 537, 974, 1080, 1165, 1307, 1832
PflMI	CCANNNNNTGG	1 cut(s)	1223
PkrI	GCNGC	8 cut(s)	45, 299, 326, 1047, 1630, 2104, 2341, 2531
PleI	GAGTC	3 cut(s)	1161, 1221, 2242
PpsI	GAGTC	3 cut(s)	1161, 1221, 2242
PscI	ACATGT	1 cut(s)	730
PsiI	TTATAA	1 cut(s)	1391
Psp124BI	GAGCTC	2 cut(s)	402, 1330
Psp6I	CCWGG	1 cut(s)	2361
PspFI	CCCAGC	1 cut(s)	139
PspGI	CCWGG	1 cut(s)	2361
PspN4I	GGNNCC	7 cut(s)	249, 288, 314, 670, 1173, 1924, 1933
PspPI	GGNCC	3 cut(s)	313, 526, 1039
PstI	CTGCAG	3 cut(s)	973, 1035, 1633
PstNI	CAGNNNCTG	1 cut(s)	2036
PsuI	RGATCY	4 cut(s)	247, 621, 1063, 1171
PteI	GCGCGC	1 cut(s)	6
PvuII	CAGCTG	1 cut(s)	2399
RsaI	GTAC	4 cut(s)	693, 922, 2064, 2350
RsaNI	GTAC	4 cut(s)	692, 921, 2063, 2349
RseI	CAYNNNNRTG	1 cut(s)	2544
SacI	GAGCTC	2 cut(s)	402, 1330
SapI	GCTCTTC	1 cut(s)	129
SatI	GCNGC	8 cut(s)	44, 298, 325, 1046, 1629, 2103, 2340, 2530
Sau96I	GGNCC	3 cut(s)	313, 526, 1039
SchI	GAGTC	3 cut(s)	1162, 1221, 2243
ScrFI	CCNGG	1 cut(s)	2363
SduI	GDGCHC	2 cut(s)	402, 1330
SfcI	CTRYAG	4 cut(s)	291, 969, 1031, 1629
SfuI	TTCGAA	2 cut(s)	32, 514
SgsI	GGCGCGCC	1 cut(s)	6
SinI	GGWCC	2 cut(s)	313, 1039
SmiMI	CAYNNNNRTG	1 cut(s)	2544
SmlI	CTYRAG	2 cut(s)	437, 674
SmoI	CTYRAG	2 cut(s)	437, 674
SphI	GCATGC	1 cut(s)	2450
SsiI	CCGC	4 cut(s)	1935, 2075, 2186, 2251
SspI	AATATT	3 cut(s)	257, 636, 1491
SspMI	CTAG	7 cut(s)	831, 945, 1304, 1910, 1953, 2285, 2523
SstI	GAGCTC	2 cut(s)	402, 1330
StyD4I	CCNGG	1 cut(s)	2361
StyI	CCWWGG	1 cut(s)	2126
TaiI	ACGT	2 cut(s)	797, 1663
TaqI	TCGA	9 cut(s)	32, 38, 190, 450, 514, 1347, 1636, 1719, 1744
TaqII	GACCGA	1 cut(s)	2294
TatI	WGTACW	2 cut(s)	2062, 2348
TfiI	GAWTC	7 cut(s)	192, 537, 974, 1080, 1165, 1307, 1832
TscAI	CASTG	6 cut(s)	382, 391, 1277, 1910, 2020, 2373
TseFI	GTSAC	1 cut(s)	106
TseI	GCWGC	8 cut(s)	43, 297, 324, 1045, 1628, 2102, 2339, 2529
Tsp45I	GTSAC	1 cut(s)	106
TspRI	CASTG	6 cut(s)	382, 391, 1277, 1910, 2020, 2373
Van91I	CCANNNNNTGG	1 cut(s)	1223
Vha464I	CTTAAG	1 cut(s)	437
VpaK11BI	GGWCC	2 cut(s)	313, 1039
XapI	RAATTY	4 cut(s)	34, 985, 1287, 1864
XbaI	TCTAGA	1 cut(s)	2522
XceI	RCATGY	3 cut(s)	734, 1270, 2450
XmiI	GTMKAC	2 cut(s)	340, 1300
XmnI	GAANNNNTTC	2 cut(s)	635, 1290
XspI	CTAG	7 cut(s)	831, 945, 1304, 1910, 1953, 2285, 2523
Zsp2I	ATGCAT	1 cut(s)	1726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.