MD13G1166100.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014383
Physical Location & Seq
Forward (+)
13250956 .. 13252641
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1166100.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGTTGAAGCAGCAGAGCAATCCCAAAAGTACCCGACAATGGCAGAGGTGGTGGAAGAACTTCACAGAATGACACATATTGGCTTCCCAATTGCGGCCATAAATTTAGCGGGTTACCTCAAAAACATGGTCCTAGTTGTGTGCATGGGAAGGCTTGGAAGTCTTGAGCTAGCAGGTGGGGCTTTAGCCATTGGCTTCACCAACATCACTGGCTACTCAGTCCTCTCTGGCTTGGCCATGGGCATGGAGCCTCTATGCAGCCAAGCTTTTGGTTCACAAAACTTCTCCATAGCTTTCCATACTTTACAAAGAACTATTCTCTTATTGCTTCTCACTTCTCTTCTAATTGCTTTGCTTTGGTCCAATCTTGAACCTCTATTGCTCCTTCTCCATCAAAACCAAGATATAACAAGGATTGCAAGCTTGTATTGCCAATTTGCCATCCCCGATCTTGTTACCAATAGCTTTCTTCACCCCATACGTATTTTTTTACGTAGTCAAAGCACAACATGGCCATTGATGTGGTCCACTTTACTAGCAATCATTCTACACATTCCTCTCACAATCTTCTTAACCTTCACTCTTACCCTTGGGGTCAAAGGAATAGCAATTTCCACTTCTCTTACCAATTTCATAACTCTATTTTTTCTTGTGGGTTACATGGTCTATGACCATATCACGAGAAAGTCTTTTTATTATTTGGTTACGGACCGGTGTGAACCCACTACTAAGTATGATACATCGCAAACATTGTACCAACCCTTATTACCTAAAAAAGTAGTACCACTTTCGTTGAGGAAAGTGCTAGGAGATGACGAATGGAGGATGCTAATTCGACTTGCTTTGCAAAGTTGCCTAGGAGTTTGCTTAGAATGGTGGTGGTACGAGTTCATGACAATTCTAGCCGGTTACCTTCACAAACCTCACATTGCCCTAGCCACATCAGCCATAGTGATACAAACCACTTCTCTCATGTACACATTGCCAACGACACTAAGTGCATCAGTGTCTACACGAGTAGGCAACGAGCTAGGGGCAGGCCAACCCAAAAAGGCACGTTTGGCGGCGGTGGTGGCGGTGGGGATGGCACTAGTGAGCTCATTGTTGGGCTTGTTATTGACCACTCTGGGGAGAGAAGCATGGGGGAGAATTTTCACAGATGATAATGAGGTTTTGGAGCTAACAATGGGTGTGCTACCAATTGTTGGACTATGTGAGATTGCAAATTGTCCACAAACTACAAGTTGTGGGATTTTGAGAGGAAGTGCTAGGCCAGGCATTGGGGCATGGATTAACTTTTACTCATTTTACATGGTAGGTGCGCCTATTGCAATAGTCTTGACGTTTGTTTGGAGATTAGGGTTCAAAGGGCTTTGTTATGGGCTTCTAGCAGCTCAAGTTACTTGTGTGGTGTCTATATTAACAGTGGTCCATAAGACAGATTGGGAGAAAGAATGGCTTAAGACGAAAGAGTTTGTAGGAAAGAATAATGATGGAATGGCTGCATTTGCACATGCAGATGAAACAGTCAAATGTGAAGAGGGTGTTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

519

Amino Acids

57.16

Weight (kDa)

7.55

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 30 - 188 6.7e-32 MatE
MatE PF01554 285 - 445 2.3e-32 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49130
fragaria_vesca FvH4_4g18550
malus_domestica MD13G1166100.v1.1 MD16G1165600.v1.1
prunus_persica Prupe.1G132600_v2.0.a1
pyrus_communis pycom16g14030
rosa_chinensis RchiOBHm_Chr4g0423121
rosa_laevigata RLG00000007582
rosa_multiflora Rmu_sc0000744.1_g000050 Rmu_sc0003669.1_g000032
rosa_roxburghii Rroxscaffold_5G00365200
rosa_rugosa Rorug04G0184100
rosa_samantha Rh4AG244000 Rh4BG249100 Rh4CG258300 Rh4DG243600
rosa_wichuraiana Rw4G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
AccB7I CCANNNNNTGG 2 cut(s) 1205, 1280
AccI GTMKAC 1 cut(s) 1010
AciI CCGC 5 cut(s) 95, 110, 1064, 1067, 1076
AcoI YGGCCR 3 cut(s) 96, 234, 512
AcsI RAATTY 2 cut(s) 103, 1149
AdeI CACNNNGTG 1 cut(s) 998
AfaI GTAC 5 cut(s) 32, 755, 783, 884, 977
AfiI CCNNNNNNNGG 5 cut(s) 40, 94, 1128, 1205, 1280
AflII CTTAAG 1 cut(s) 1460
AgeI ACCGGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 8, 371, 1366
AhlI ACTAGT 1 cut(s) 1090
AjnI CCWGG 1 cut(s) 1273
AluBI AGCT 9 cut(s) 169, 266, 293, 423, 465, 1030, 1098, 1180, 1394
AluI AGCT 9 cut(s) 169, 266, 293, 423, 465, 1030, 1098, 1180, 1394
Alw21I GWGCWC 1 cut(s) 1100
AoxI GGCC 5 cut(s) 96, 234, 512, 1039, 1271
ApeKI GCWGC 4 cut(s) 11, 258, 1391, 1502
ApoI RAATTY 2 cut(s) 103, 1149
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AsiGI ACCGGT 1 cut(s) 711
Asp700I GAANNNNTTC 1 cut(s) 60
AspA2I CCTAGG 1 cut(s) 856
AspLEI GCGC 1 cut(s) 1324
AspS9I GGNCC 5 cut(s) 130, 360, 525, 709, 1429
AsuHPI GGTGA 2 cut(s) 190, 464
AsuNHI GCTAGC 1 cut(s) 169
AvaII GGWCC 5 cut(s) 130, 360, 525, 709, 1429
AvrII CCTAGG 1 cut(s) 856
BalI TGGCCA 2 cut(s) 236, 514
BanII GRGCYC 1 cut(s) 1100
BauI CACGAG 2 cut(s) 679, 1014
Bbv12I GWGCWC 1 cut(s) 1100
BbvI GCAGC 4 cut(s) 23, 270, 1403, 1489
BccI CCATC 4 cut(s) 399, 449, 1078, 1487
BciT130I CCWGG 1 cut(s) 1275
BcuI ACTAGT 1 cut(s) 1090
BfrI CTTAAG 1 cut(s) 1460
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 6 cut(s) 12, 96, 259, 1065, 1392, 1503
BlnI CCTAGG 1 cut(s) 856
BlsI GCNGC 6 cut(s) 13, 97, 260, 1066, 1393, 1504
Bme1390I CCNGG 1 cut(s) 1275
Bme18I GGWCC 5 cut(s) 130, 360, 525, 709, 1429
BmgT120I GGNCC 5 cut(s) 130, 360, 525, 709, 1429
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 1275
BmsI GCATC 2 cut(s) 816, 1010
BmtI GCTAGC 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 185, 1380
BsaAI YACGTR 2 cut(s) 482, 494
BsaJI CCNNGG 3 cut(s) 237, 589, 856
BsaWI WCCGGW 1 cut(s) 711
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 94, 1128, 1205, 1280
Bse118I RCCGGY 2 cut(s) 711, 905
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 2 cut(s) 927, 980
BseBI CCWGG 1 cut(s) 1275
BseDI CCNNGG 3 cut(s) 237, 589, 856
BseGI GGATG 3 cut(s) 441, 831, 1089
BseLI CCNNNNNNNGG 5 cut(s) 40, 94, 1128, 1205, 1280
BseMI GCAATG 2 cut(s) 927, 980
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 4 cut(s) 23, 270, 1403, 1489
BshFI GGCC 5 cut(s) 98, 236, 514, 1041, 1273
BshTI ACCGGT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 1100
BsiSI CCGG 2 cut(s) 712, 906
BslI CCNNNNNNNGG 5 cut(s) 40, 94, 1128, 1205, 1280
BsnI GGCC 5 cut(s) 98, 236, 514, 1041, 1273
Bsp1286I GDGCHC 1 cut(s) 1100
Bsp1407I TGTACA 1 cut(s) 975
Bsp143I GATC 1 cut(s) 448
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 5 cut(s) 95, 110, 1064, 1067, 1076
BspANI GGCC 5 cut(s) 98, 236, 514, 1041, 1273
BspCNI CTCAG 1 cut(s) 230
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 249
BspMI ACCTGC 1 cut(s) 164
BspOI GCTAGC 1 cut(s) 173
BspTI CTTAAG 1 cut(s) 1460
BsrDI GCAATG 2 cut(s) 927, 980
BsrFI RCCGGY 2 cut(s) 711, 905
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 2 cut(s) 711, 905
BssECI CCNNGG 3 cut(s) 237, 589, 856
BssMI GATC 1 cut(s) 448
BssSI CACGAG 2 cut(s) 679, 1014
BssT1I CCWWGG 3 cut(s) 237, 589, 856
Bst2BI CACGAG 2 cut(s) 679, 1014
Bst2UI CCWGG 1 cut(s) 1275
Bst4CI ACNGT 2 cut(s) 1426, 1528
Bst6I CTCTTC 2 cut(s) 345, 1533
BstAFI CTTAAG 1 cut(s) 1460
BstAUI TGTACA 1 cut(s) 975
BstBAI YACGTR 2 cut(s) 482, 494
BstC8I GCNNGC 3 cut(s) 171, 421, 1039
BstDEI CTNAG 4 cut(s) 217, 729, 868, 995
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 113, 908
BstF5I GGATG 3 cut(s) 441, 831, 1089
BstHHI GCGC 1 cut(s) 1324
BstKTI GATC 1 cut(s) 451
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 7 cut(s) 179, 429, 944, 1061, 1073, 1328, 1508
BstNI CCWGG 1 cut(s) 1275
BstNSI RCATGY 1 cut(s) 1517
BstPI GGTNACC 2 cut(s) 113, 908
BstSCI CCNGG 1 cut(s) 1273
BstSNI TACGTA 2 cut(s) 482, 494
BstV1I GCAGC 4 cut(s) 23, 270, 1403, 1489
BstXI CCANNNNNNTGG 3 cut(s) 244, 269, 522
BsuRI GGCC 5 cut(s) 98, 236, 514, 1041, 1273
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 3 cut(s) 441, 831, 1089
BtsIMutI CAGTG 3 cut(s) 207, 1011, 1431
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 171, 421, 1039
CciI TCATGA 1 cut(s) 891
CfoI GCGC 1 cut(s) 1324
Cfr10I RCCGGY 2 cut(s) 711, 905
Cfr13I GGNCC 5 cut(s) 130, 360, 525, 709, 1429
CpoI CGGWCCG 1 cut(s) 709
Csp6I GTAC 5 cut(s) 31, 754, 782, 883, 976
CspAI ACCGGT 1 cut(s) 711
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CspI CGGWCCG 1 cut(s) 709
CviQI GTAC 5 cut(s) 31, 754, 782, 883, 976
DdeI CTNAG 4 cut(s) 217, 729, 868, 995
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DraIII CACNNNGTG 1 cut(s) 998
EaeI YGGCCR 3 cut(s) 96, 234, 512
Eam1104I CTCTTC 2 cut(s) 345, 1533
EarI CTCTTC 2 cut(s) 345, 1533
Ecl136II GAGCTC 1 cut(s) 1098
Eco105I TACGTA 2 cut(s) 482, 494
Eco130I CCWWGG 3 cut(s) 237, 589, 856
Eco24I GRGCYC 1 cut(s) 1100
Eco47I GGWCC 5 cut(s) 130, 360, 525, 709, 1429
Eco53kI GAGCTC 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 113, 908
EcoICRI GAGCTC 1 cut(s) 1098
EcoO65I GGTNACC 2 cut(s) 113, 908
EcoRII CCWGG 1 cut(s) 1273
EcoT14I CCWWGG 3 cut(s) 237, 589, 856
EcoT38I GRGCYC 1 cut(s) 1100
ErhI CCWWGG 3 cut(s) 237, 589, 856
FalI AAGNNNNNCTT 2 cut(s) 1443, 1475
FauI CCCGC 1 cut(s) 103
FblI GTMKAC 1 cut(s) 1010
Fnu4HI GCNGC 6 cut(s) 12, 96, 259, 1065, 1392, 1503
FokI GGATG 3 cut(s) 428, 838, 1096
FriOI GRGCYC 1 cut(s) 1100
Fsp4HI GCNGC 6 cut(s) 12, 96, 259, 1065, 1392, 1503
GlaI GCGC 1 cut(s) 1323
GluI GCNGC 6 cut(s) 12, 96, 259, 1065, 1392, 1503
HaeIII GGCC 5 cut(s) 98, 236, 514, 1041, 1273
HapII CCGG 2 cut(s) 712, 906
HhaI GCGC 1 cut(s) 1324
Hin6I GCGC 1 cut(s) 1322
HinP1I GCGC 1 cut(s) 1322
HindIII AAGCTT 2 cut(s) 264, 421
HpaII CCGG 2 cut(s) 712, 906
HphI GGTGA 2 cut(s) 190, 464
Hpy166II GTNNAC 6 cut(s) 275, 528, 719, 978, 1011, 1232
Hpy188III TCNNGA 5 cut(s) 164, 368, 679, 892, 1339
Hpy8I GTNNAC 6 cut(s) 275, 528, 719, 978, 1011, 1232
HpyAV CCTTC 4 cut(s) 144, 395, 586, 923
HpyCH4III ACNGT 2 cut(s) 1426, 1528
HpyCH4IV ACGT 4 cut(s) 481, 493, 1057, 1343
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 429, 944, 1061, 1073, 1328, 1508
HpyF3I CTNAG 4 cut(s) 217, 729, 868, 995
HpySE526I ACGT 4 cut(s) 481, 493, 1057, 1343
HspAI GCGC 1 cut(s) 1322
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 3 cut(s) 247, 387, 1177
LpnPI CCDG 9 cut(s) 159, 195, 213, 725, 919, 1023, 1112, 1260, 1287
Lsp1109I GCAGC 4 cut(s) 23, 270, 1403, 1489
LweI GCATC 2 cut(s) 816, 1010
MaeII ACGT 4 cut(s) 481, 493, 1057, 1343
MaeIII GTNAC 6 cut(s) 113, 454, 656, 703, 908, 1399
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 5 cut(s) 68, 332, 461, 559, 1550
MfeI CAATTG 2 cut(s) 90, 1200
MhlI GDGCHC 1 cut(s) 1100
MlsI TGGCCA 2 cut(s) 236, 514
MluNI TGGCCA 2 cut(s) 236, 514
MmeI TCCRAC 1 cut(s) 1186
Mox20I TGGCCA 2 cut(s) 236, 514
MroXI GAANNNNTTC 1 cut(s) 60
MscI TGGCCA 2 cut(s) 236, 514
MseI TTAA 4 cut(s) 572, 1293, 1421, 1461
MslI CAYNNNNRTG 3 cut(s) 242, 520, 1518
Msp20I TGGCCA 2 cut(s) 236, 514
MspCI CTTAAG 1 cut(s) 1460
MspI CCGG 2 cut(s) 712, 906
MspR9I CCNGG 1 cut(s) 1275
MunI CAATTG 2 cut(s) 90, 1200
MvaI CCWGG 1 cut(s) 1275
MwoI GCNNNNNNNGC 7 cut(s) 179, 429, 944, 1061, 1073, 1328, 1508
NcoI CCATGG 1 cut(s) 237
NdeII GATC 1 cut(s) 448
NheI GCTAGC 1 cut(s) 169
NlaIV GGNNCC 1 cut(s) 249
NspI RCATGY 1 cut(s) 1517
PagI TCATGA 1 cut(s) 891
PaqCI CACCTGC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 60
PflMI CCANNNNNTGG 2 cut(s) 1205, 1280
PinAI ACCGGT 1 cut(s) 711
PkrI GCNGC 6 cut(s) 13, 97, 260, 1066, 1393, 1504
Ppu21I YACGTR 2 cut(s) 482, 494
Psp124BI GAGCTC 1 cut(s) 1100
Psp6I CCWGG 1 cut(s) 1273
PspEI GGTNACC 2 cut(s) 113, 908
PspGI CCWGG 1 cut(s) 1273
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 5 cut(s) 130, 360, 525, 709, 1429
RsaI GTAC 5 cut(s) 32, 755, 783, 884, 977
RsaNI GTAC 5 cut(s) 31, 754, 782, 883, 976
RseI CAYNNNNRTG 3 cut(s) 242, 520, 1518
Rsr2I CGGWCCG 1 cut(s) 709
RsrII CGGWCCG 1 cut(s) 709
SacI GAGCTC 1 cut(s) 1100
SaqAI TTAA 4 cut(s) 572, 1293, 1421, 1461
SatI GCNGC 6 cut(s) 12, 96, 259, 1065, 1392, 1503
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 5 cut(s) 130, 360, 525, 709, 1429
ScrFI CCNGG 1 cut(s) 1275
SduI GDGCHC 1 cut(s) 1100
SfaNI GCATC 2 cut(s) 816, 1010
SinI GGWCC 5 cut(s) 130, 360, 525, 709, 1429
SmiMI CAYNNNNRTG 3 cut(s) 242, 520, 1518
SmlI CTYRAG 3 cut(s) 164, 1395, 1460
SmoI CTYRAG 3 cut(s) 164, 1395, 1460
SnaBI TACGTA 2 cut(s) 482, 494
SpeI ACTAGT 1 cut(s) 1090
SsiI CCGC 5 cut(s) 95, 110, 1064, 1067, 1076
SstI GAGCTC 1 cut(s) 1100
StyD4I CCNGG 1 cut(s) 1273
StyI CCWWGG 3 cut(s) 237, 589, 856
TaaI ACNGT 2 cut(s) 1426, 1528
TaiI ACGT 4 cut(s) 484, 496, 1060, 1346
TaqI TCGA 1 cut(s) 835
TatI WGTACW 1 cut(s) 975
TauI GCSGC 2 cut(s) 98, 1067
Tru1I TTAA 4 cut(s) 572, 1293, 1421, 1461
Tru9I TTAA 4 cut(s) 572, 1293, 1421, 1461
TscAI CASTG 3 cut(s) 214, 1011, 1431
TseI GCWGC 4 cut(s) 11, 258, 1391, 1502
TspDTI ATGAA 3 cut(s) 622, 880, 1536
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 3 cut(s) 214, 1011, 1431
Van91I CCANNNNNTGG 2 cut(s) 1205, 1280
Vha464I CTTAAG 1 cut(s) 1460
VpaK11BI GGWCC 5 cut(s) 130, 360, 525, 709, 1429
XapI RAATTY 2 cut(s) 103, 1149
XceI RCATGY 1 cut(s) 1517
XmaJI CCTAGG 1 cut(s) 856
XmiI GTMKAC 1 cut(s) 1010
XmnI GAANNNNTTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.