MD16G1165600.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014383
Physical Location & Seq
Forward (+)
13796450 .. 13798358
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1165600.v1.1.491

Sequence Viewer

Length: 1557 bp
ATGGTTGAAGCAGCAGAGCCATCCCACAAGTGCCCGACAATGCCAGAGGTGGTGGAAGAACTTCACAGAATGTCAAATATTGGCTTCCCAATTGCGGCCATGAGTTTAGTGGGTTACCTCAAAAACATGGTCCTAGTTGTATGCATGGGAAGGCTTGGAAGTCTTGAGCTAGCAGGTGGAGCTTTAGGCATTGGCTTCACCAACATCACTGGCTACTCAGTCCTCTCTGGCTTGGCCATGGGTATGGAGCCTCTATGCAGCCAAGCTTTTGGTTCACAGAACTTCTCCATAGCTTTCCACACTTTACAAAGAACCATTCTCTTATTGCTTCTCACTTCTCTTCCTATTGGTTTGCTTTGGGCCAATCTTGAACCTCTCATGCTCCTCCTCCATCAAAACCAAGATATAACAAAGATTGCTAGCTTGTATTGCCAATTTGCCATCCCGGATCTTCTTGCCAATAGCCTTCTTCATCCCATACGTATTTTTTTACGTAGTCAAAGCACAACATGGCCATTGATGTGGTCCACTTTACTAGCAACCATTCTACACATTCCTCTCACAATCTTCTTAACCTTCAATCTTTCCCTTGGGATCAAAGGAATAGCAATTTCCACTTCTCTTACCAATTTTATCACTCTATTTTTTCTTTTGGGCTATGTGGTCTACGCCCATGTCACAAAAAAATCTATTTATTGTTTGGTAACGGACCAGTGTGAACCCACTACTAAGTATGAAACAACACAATTATTGTCCCAGCCATTATTACCCAAAAAAATAATACCACTTTCGTTGAGGAAAACACTAGGAGCTGACGAATGGAGGATCCTAATTCGACTTGCTTTCCAAAGTTGCTTAGGAGTTTGCTTAGAATGGTGGTGGTACGAGTTCATGACAATTCTAACTGGTTACCTTCAAAAACCTCACATTGCCCTGGCAGCATCAGCCATAGTGATACAAACCACATCTCTCATGTACACATTACCAGCGGCACTAAGTGCATCAGTGTCTACACGAGTAGGCAACGAGCTAGGGGCAGGCCAACCCAAAAAGGCACATTTAGCGGCAGTGGTGGCCGTGGGGATGGCACTAGTGAGCTCATTGTTGGGCTTGTCATTGACCACTCTAGGGAGAGGAGTATGGGGGAGAATCTTCACAAATGATAGTGAGGTTTTGGAGCTAACAATGAGTGTGCTACCAATTATTGGACTATGTGAGATTGCTAATTGTCCACAAACTACAAGTTGTGGGATTTTGAGAGGAAGTGCTAGGCCAGGCATTGGGGCATGGATTAACTTTTACTCATTTTACATGGTGGGTACGCCTGTGGCAATAGTCTTGACGTTTGTGTGGAGGTTAGGGTTTAAAGGGCTTTGTTATGGACTTCTAGCAGCTCAAATTACATGTGTGGTGTCCATATTAACAGTGGTTCATAAGACAGATTGGGAGAGAGAATGTCTCAAGACGAAAGAGCTTGTAGGAAAGAATAATGACAGAATGGCTGCATTTGCACATGCAGATGAAACAGTCAAATGTGAAGAGGGTGCTGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

519

Amino Acids

56.58

Weight (kDa)

8.3

Isoelectric Point (pI)

32.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 30 - 188 1e-32 MatE
MatE PF01554 285 - 445 1.1e-31 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49130
fragaria_vesca FvH4_4g18550
malus_domestica MD13G1166100.v1.1 MD16G1165600.v1.1
prunus_persica Prupe.1G132600_v2.0.a1
pyrus_communis pycom16g14030
rosa_chinensis RchiOBHm_Chr4g0423121
rosa_laevigata RLG00000007582
rosa_multiflora Rmu_sc0000744.1_g000050 Rmu_sc0003669.1_g000032
rosa_roxburghii Rroxscaffold_5G00365200
rosa_rugosa Rorug04G0184100
rosa_samantha Rh4AG244000 Rh4BG249100 Rh4CG258300 Rh4DG243600
rosa_wichuraiana Rw4G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 164
Acc36I ACCTGC 1 cut(s) 164
AccB7I CCANNNNNTGG 2 cut(s) 1205, 1280
AccI GTMKAC 2 cut(s) 666, 1010
AciI CCGC 3 cut(s) 95, 989, 1064
AclWI GGATC 4 cut(s) 456, 602, 820, 833
AcoI YGGCCR 4 cut(s) 96, 234, 512, 1074
AdeI CACNNNGTG 1 cut(s) 998
AfaI GTAC 3 cut(s) 884, 977, 1321
AfiI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
AflIII ACRYGT 1 cut(s) 1403
AgsI TTSAA 4 cut(s) 8, 371, 580, 917
AhlI ACTAGT 1 cut(s) 1090
AjnI CCWGG 2 cut(s) 933, 1273
Alw21I GWGCWC 1 cut(s) 1100
Alw26I GTCTC 1 cut(s) 1463
AlwI GGATC 4 cut(s) 456, 602, 820, 833
AoxI GGCC 7 cut(s) 96, 234, 360, 512, 1039, 1074, 1271
ApeKI GCWGC 6 cut(s) 11, 258, 938, 1391, 1502, 1547
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
Asp700I GAANNNNTTC 1 cut(s) 60
AspS9I GGNCC 4 cut(s) 130, 360, 525, 709
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 1 cut(s) 190
AsuNHI GCTAGC 2 cut(s) 169, 419
AvaII GGWCC 3 cut(s) 130, 525, 709
BaeGI GKGCMC 1 cut(s) 35
BaeI ACNNNNGTAYC 2 cut(s) 1311, 1344
BalI TGGCCA 2 cut(s) 236, 514
BamHI GGATCC 1 cut(s) 825
BanII GRGCYC 1 cut(s) 1100
BauI CACGAG 1 cut(s) 1014
Bbv12I GWGCWC 1 cut(s) 1100
BbvI GCAGC 6 cut(s) 23, 270, 950, 1403, 1489, 1534
BccI CCATC 4 cut(s) 28, 399, 449, 1078
BceAI ACGGC 1 cut(s) 1061
BciT130I CCWGG 2 cut(s) 935, 1275
BcnI CCSGG 1 cut(s) 446
BcoDI GTCTC 1 cut(s) 1463
BcuI ACTAGT 1 cut(s) 1090
BfuAI ACCTGC 1 cut(s) 164
BisI GCNGC 9 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503, 1548
BlsI GCNGC 9 cut(s) 13, 97, 260, 940, 991, 1066, 1393, 1504, 1549
Bme1390I CCNGG 3 cut(s) 446, 935, 1275
Bme18I GGWCC 3 cut(s) 130, 525, 709
BmgT120I GGNCC 4 cut(s) 130, 360, 525, 709
BmiI GGNNCC 2 cut(s) 249, 827
BmrFI CCNGG 3 cut(s) 446, 935, 1275
BmsI GCATC 2 cut(s) 950, 1010
BmtI GCTAGC 2 cut(s) 173, 423
BplI GAGNNNNNCTC 2 cut(s) 1443, 1475
Bpu10I CCTNAGC 1 cut(s) 856
BpuEI CTTGAG 2 cut(s) 185, 1445
BpuMI CCSGG 1 cut(s) 446
BsaAI YACGTR 2 cut(s) 482, 494
BsaJI CCNNGG 4 cut(s) 237, 589, 933, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
Bse1I ACTGG 3 cut(s) 214, 712, 910
Bse3DI GCAATG 1 cut(s) 927
BseBI CCWGG 2 cut(s) 935, 1275
BseDI CCNNGG 4 cut(s) 237, 589, 933, 1077
BseGI GGATG 4 cut(s) 20, 441, 472, 1089
BseLI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 3 cut(s) 214, 712, 910
BseRI GAGGAG 3 cut(s) 374, 377, 1149
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 6 cut(s) 23, 270, 950, 1403, 1489, 1534
BseYI CCCAGC 1 cut(s) 756
BshFI GGCC 7 cut(s) 98, 236, 362, 514, 1041, 1076, 1273
BsiHKAI GWGCWC 1 cut(s) 1100
BsiSI CCGG 1 cut(s) 446
BslFI GGGAC 1 cut(s) 739
BslI CCNNNNNNNGG 4 cut(s) 94, 1128, 1205, 1280
BsmAI GTCTC 1 cut(s) 1463
BsmFI GGGAC 1 cut(s) 739
BsnI GGCC 7 cut(s) 98, 236, 362, 514, 1041, 1076, 1273
Bsp1286I GDGCHC 2 cut(s) 35, 1100
Bsp1407I TGTACA 1 cut(s) 975
Bsp143I GATC 3 cut(s) 448, 594, 825
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 3 cut(s) 95, 989, 1064
BspANI GGCC 7 cut(s) 98, 236, 362, 514, 1041, 1076, 1273
BspCNI CTCAG 1 cut(s) 230
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 2 cut(s) 249, 827
BspMI ACCTGC 1 cut(s) 164
BspOI GCTAGC 2 cut(s) 173, 423
BspPI GGATC 4 cut(s) 456, 602, 820, 833
BsrDI GCAATG 1 cut(s) 927
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 3 cut(s) 214, 712, 910
BssECI CCNNGG 4 cut(s) 237, 589, 933, 1077
BssMI GATC 3 cut(s) 448, 594, 825
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 2 cut(s) 237, 589
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 2 cut(s) 935, 1275
Bst4CI ACNGT 2 cut(s) 1426, 1528
Bst6I CTCTTC 2 cut(s) 345, 1533
BstAPI GCANNNNNTGC 1 cut(s) 998
BstAUI TGTACA 1 cut(s) 975
BstBAI YACGTR 2 cut(s) 482, 494
BstC8I GCNNGC 3 cut(s) 171, 421, 1039
BstDEI CTNAG 5 cut(s) 217, 729, 856, 868, 995
BstDSI CCRYGG 2 cut(s) 237, 1077
BstEII GGTNACC 2 cut(s) 113, 908
BstF5I GGATG 4 cut(s) 20, 441, 472, 1089
BstKTI GATC 3 cut(s) 451, 597, 828
BstMAI GTCTC 1 cut(s) 1463
BstMBI GATC 3 cut(s) 448, 594, 825
BstMWI GCNNNNNNNGC 8 cut(s) 179, 429, 938, 944, 998, 1061, 1073, 1508
BstNI CCWGG 2 cut(s) 935, 1275
BstNSI RCATGY 2 cut(s) 1407, 1517
BstPI GGTNACC 2 cut(s) 113, 908
BstSCI CCNGG 3 cut(s) 444, 933, 1273
BstSLI GKGCMC 1 cut(s) 35
BstSNI TACGTA 2 cut(s) 482, 494
BstV1I GCAGC 6 cut(s) 23, 270, 950, 1403, 1489, 1534
BstX2I RGATCY 2 cut(s) 448, 825
BstXI CCANNNNNNTGG 3 cut(s) 244, 269, 522
BstYI RGATCY 2 cut(s) 448, 825
BsuRI GGCC 7 cut(s) 98, 236, 362, 514, 1041, 1076, 1273
BtgI CCRYGG 2 cut(s) 237, 1077
BtsCI GGATG 4 cut(s) 20, 441, 472, 1089
BtsI GCAGTG 1 cut(s) 1074
BtsIMutI CAGTG 5 cut(s) 207, 719, 1011, 1074, 1431
BveI ACCTGC 1 cut(s) 164
Cac8I GCNNGC 3 cut(s) 171, 421, 1039
CciI TCATGA 1 cut(s) 891
Cfr13I GGNCC 4 cut(s) 130, 360, 525, 709
Csp6I GTAC 3 cut(s) 883, 976, 1320
CspCI CAANNNNNGTGG 2 cut(s) 774, 809
CviQI GTAC 3 cut(s) 883, 976, 1320
DdeI CTNAG 5 cut(s) 217, 729, 856, 868, 995
DpnI GATC 3 cut(s) 450, 596, 827
DpnII GATC 3 cut(s) 448, 594, 825
DraI TTTAAA 1 cut(s) 1366
DraIII CACNNNGTG 1 cut(s) 998
EaeI YGGCCR 4 cut(s) 96, 234, 512, 1074
Eam1104I CTCTTC 2 cut(s) 345, 1533
EarI CTCTTC 2 cut(s) 345, 1533
Ecl136II GAGCTC 1 cut(s) 1098
Eco105I TACGTA 2 cut(s) 482, 494
Eco130I CCWWGG 2 cut(s) 237, 589
Eco24I GRGCYC 1 cut(s) 1100
Eco47I GGWCC 3 cut(s) 130, 525, 709
Eco53kI GAGCTC 1 cut(s) 1098
Eco91I GGTNACC 2 cut(s) 113, 908
EcoICRI GAGCTC 1 cut(s) 1098
EcoO65I GGTNACC 2 cut(s) 113, 908
EcoRII CCWGG 2 cut(s) 933, 1273
EcoT14I CCWWGG 2 cut(s) 237, 589
EcoT22I ATGCAT 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 1100
ErhI CCWWGG 2 cut(s) 237, 589
FaqI GGGAC 1 cut(s) 739
FblI GTMKAC 2 cut(s) 666, 1010
Fnu4HI GCNGC 9 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503, 1548
FokI GGATG 4 cut(s) 7, 428, 459, 1096
FriOI GRGCYC 1 cut(s) 1100
Fsp4HI GCNGC 9 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503, 1548
GluI GCNGC 9 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503, 1548
GsaI CCCAGC 1 cut(s) 760
HaeIII GGCC 7 cut(s) 98, 236, 362, 514, 1041, 1076, 1273
HapII CCGG 1 cut(s) 446
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 1 cut(s) 1149
HpaII CCGG 1 cut(s) 446
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 7 cut(s) 275, 528, 667, 719, 978, 1011, 1232
Hpy188III TCNNGA 5 cut(s) 164, 368, 892, 1339, 1462
Hpy8I GTNNAC 7 cut(s) 275, 528, 667, 719, 978, 1011, 1232
HpyAV CCTTC 4 cut(s) 144, 476, 586, 923
HpyCH4III ACNGT 2 cut(s) 1426, 1528
HpyCH4IV ACGT 3 cut(s) 481, 493, 1343
HpyCH4V TGCA 7 cut(s) 144, 258, 1001, 1505, 1511, 1517, 1550
HpyF10VI GCNNNNNNNGC 8 cut(s) 179, 429, 938, 944, 998, 1061, 1073, 1508
HpyF3I CTNAG 5 cut(s) 217, 729, 856, 868, 995
HpySE526I ACGT 3 cut(s) 481, 493, 1343
Kzo9I GATC 3 cut(s) 448, 594, 825
LmnI GCTCC 5 cut(s) 179, 247, 387, 809, 1177
Lsp1109I GCAGC 6 cut(s) 23, 270, 950, 1403, 1489, 1534
LweI GCATC 2 cut(s) 950, 1010
MaeII ACGT 3 cut(s) 481, 493, 1343
MaeIII GTNAC 4 cut(s) 113, 676, 703, 908
MalI GATC 3 cut(s) 450, 596, 827
MboI GATC 3 cut(s) 448, 594, 825
MboII GAAGA 7 cut(s) 68, 332, 443, 461, 559, 1144, 1550
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 2 cut(s) 448, 825
MhlI GDGCHC 2 cut(s) 35, 1100
MlsI TGGCCA 2 cut(s) 236, 514
MluNI TGGCCA 2 cut(s) 236, 514
Mox20I TGGCCA 2 cut(s) 236, 514
Mph1103I ATGCAT 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 60
MscI TGGCCA 2 cut(s) 236, 514
MseI TTAA 4 cut(s) 572, 1293, 1365, 1421
MslI CAYNNNNRTG 3 cut(s) 242, 520, 1518
Msp20I TGGCCA 2 cut(s) 236, 514
MspA1I CMGCKG 1 cut(s) 989
MspI CCGG 1 cut(s) 446
MspR9I CCNGG 3 cut(s) 446, 935, 1275
MunI CAATTG 1 cut(s) 90
MvaI CCWGG 2 cut(s) 935, 1275
MwoI GCNNNNNNNGC 8 cut(s) 179, 429, 938, 944, 998, 1061, 1073, 1508
NciI CCSGG 1 cut(s) 446
NcoI CCATGG 1 cut(s) 237
NdeII GATC 3 cut(s) 448, 594, 825
NheI GCTAGC 2 cut(s) 169, 419
NlaIV GGNNCC 2 cut(s) 249, 827
NmuCI GTSAC 1 cut(s) 676
NsiI ATGCAT 1 cut(s) 146
NspI RCATGY 2 cut(s) 1407, 1517
PagI TCATGA 1 cut(s) 891
PaqCI CACCTGC 1 cut(s) 164
PciI ACATGT 1 cut(s) 1403
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 1149
PflMI CCANNNNNTGG 2 cut(s) 1205, 1280
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 9 cut(s) 13, 97, 260, 940, 991, 1066, 1393, 1504, 1549
Ppu21I YACGTR 2 cut(s) 482, 494
PscI ACATGT 1 cut(s) 1403
Psp124BI GAGCTC 1 cut(s) 1100
Psp6I CCWGG 2 cut(s) 933, 1273
PspEI GGTNACC 2 cut(s) 113, 908
PspFI CCCAGC 1 cut(s) 756
PspGI CCWGG 2 cut(s) 933, 1273
PspN4I GGNNCC 2 cut(s) 249, 827
PspPI GGNCC 4 cut(s) 130, 360, 525, 709
PsuI RGATCY 2 cut(s) 448, 825
RsaI GTAC 3 cut(s) 884, 977, 1321
RsaNI GTAC 3 cut(s) 883, 976, 1320
RseI CAYNNNNRTG 3 cut(s) 242, 520, 1518
SacI GAGCTC 1 cut(s) 1100
SaqAI TTAA 4 cut(s) 572, 1293, 1365, 1421
SatI GCNGC 9 cut(s) 12, 96, 259, 939, 990, 1065, 1392, 1503, 1548
Sau3AI GATC 3 cut(s) 448, 594, 825
Sau96I GGNCC 4 cut(s) 130, 360, 525, 709
ScrFI CCNGG 3 cut(s) 446, 935, 1275
SduI GDGCHC 2 cut(s) 35, 1100
SfaNI GCATC 2 cut(s) 950, 1010
SinI GGWCC 3 cut(s) 130, 525, 709
SmiMI CAYNNNNRTG 3 cut(s) 242, 520, 1518
SmlI CTYRAG 2 cut(s) 164, 1460
SmoI CTYRAG 2 cut(s) 164, 1460
SnaBI TACGTA 2 cut(s) 482, 494
SpeI ACTAGT 1 cut(s) 1090
SsiI CCGC 3 cut(s) 95, 989, 1064
SspI AATATT 1 cut(s) 79
SstI GAGCTC 1 cut(s) 1100
StyD4I CCNGG 3 cut(s) 444, 933, 1273
StyI CCWWGG 2 cut(s) 237, 589
TaaI ACNGT 2 cut(s) 1426, 1528
TaiI ACGT 3 cut(s) 484, 496, 1346
TaqI TCGA 1 cut(s) 835
TatI WGTACW 1 cut(s) 975
TauI GCSGC 3 cut(s) 98, 992, 1067
TfiI GAWTC 1 cut(s) 1149
Tru1I TTAA 4 cut(s) 572, 1293, 1365, 1421
Tru9I TTAA 4 cut(s) 572, 1293, 1365, 1421
TscAI CASTG 5 cut(s) 214, 719, 1011, 1074, 1431
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 6 cut(s) 11, 258, 938, 1391, 1502, 1547
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 5 cut(s) 461, 750, 880, 1421, 1536
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 5 cut(s) 214, 719, 1011, 1074, 1431
Van91I CCANNNNNTGG 2 cut(s) 1205, 1280
VpaK11BI GGWCC 3 cut(s) 130, 525, 709
XceI RCATGY 2 cut(s) 1407, 1517
XcmI CCANNNNNNNNNTGG 2 cut(s) 106, 1423
XmiI GTMKAC 2 cut(s) 666, 1010
XmnI GAANNNNTTC 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.