Rh4DG243600

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014383
Physical Location & Seq
Reverse (-)
45484093 .. 45486497
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG243600.1

Sequence Viewer

Length: 1608 bp
ATGGTTGCAGAAGCAGCAGAGCAATCCCAAAAGTACCCGACAATGCCAGAGGTGGTGGAGGAGCTTCACAGGATGACAGATATTGGCTTCCCCATAGCAGCCATGAGCTTGGTTGGCTACCTCAAAAACATGGTCTTAGTTGTATGCATGGGAAGACTAGGAAGTTTGGAGCTAGCAGGAGGAGCTTTAGCCATTGGCTTCACCAATATCACAGGCTACTCGGTCCTCTCTGGCTTAGCCATGGGTATGGAACCCCTCTGTAGCCAAGCCTTTGGATCCCACAACTTCTCCATGGCTTTCCTCACTTTGCAAAGAACAATTCTCTTGTTACTACTTGCTTCTCTTCCTATTTCCTTGCTTTGGGTCAATCTCGAACCTCTCATGCTCATTCTCCATCAGAACCCCGATATAACCCGAATTGCAAGCTTGTATTGCCGGTTTGCCATCCCTGATCTCCTAGCCAATAGTCTTCTTCACCCTTTGCGTATTTTCTTGCGCAGTCGAAGCATAACTTGGCCAATGATGTGGTGCACTTTACTAGCTACCATTCTTCACCTTCCTCTCACCTTTTTCTTGACCTTCACTCTTTCTCTTGGAGTCAAAGGAATAGCCATTTCCACTTTCCTCACCAATTTCCTCACTCTCTTTTTTCTTTTGGGTTACATATTGTATACCTACACCACTAAAGCACGTTTGTATTGCTGGGTAACGGACGAGCGTGAACCCACTACTAGTTCTGAACGAGCATTGTATCATAACATAAGAAATAAACCTGAACTTACAAGTAGGACTAATTCTGAACCAGCACCCATATTGTCACCATTGTTACCCGAACCGTTAGTTCCACTTTCATTGAAGAGTTCACTACTCGGAGACGGATTGGGGATGCTAATCAGACTATCAATACAAAGTTGTCTAGCAGTTTGCCTAGAATGGTGGTGGTATGAGTTCATGACAATTCTAGCTGGTTACCTTGACAAACCTCATATTGCCCTTGCAACATCAGCCATAGTAATCCAAACCACCTCTCTCATGTACACATTGCCTGCCGCCCTAAGCGCGTCGGTGTCTACAAGAGTAGGAAACGAGCTTGGGGCAGGCCAACCCGAGAAGGCCCGTTTAGCTGCGGTGGTGGCAGTGGGGATGGCACTAGTGAGCTCATTGTCGGGCTTGTCACTTACAACACTAGGAAGAGAGACATGGGGGAGAATCTTCACAAAAGATAATAAGGTTCTTGAGCTAACCGTGGCTGTCCTACCCATTATCGGTCTGTGCGAGCTCGCCAATTGTCCACAAACCACAAGCTGTGGGATATTGAGAGGTAGTGCTAGGCCTGGAATTGGGGCATGGATCAATTTCTTCTCATTCTACGTGGTGGGTGCGCCTGTGGCCATAGTGATGGGATTTGTTTGGAGTTTAGGGTTCAAGGGACTTTGTTACGGGCTTCTAGCGGCTCAAATCACATGCGTCGTGTCGATATTGACAGTGGTGCACAAGACAGATTGGGAGAGGGAGTGGCTCAAGGCTAAGGGCTCTGTGGGAAAGAATGGAAGGGATGGATTTGCACATGCAGATGATCATCAAGCAATCAAATCTGAAGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

535

Amino Acids

58.47

Weight (kDa)

7.94

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 31 - 190 1.2e-32 MatE
MatE PF01554 305 - 465 6.2e-33 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49130
fragaria_vesca FvH4_4g18550
malus_domestica MD13G1166100.v1.1 MD16G1165600.v1.1
prunus_persica Prupe.1G132600_v2.0.a1
pyrus_communis pycom16g14030
rosa_chinensis RchiOBHm_Chr4g0423121
rosa_laevigata RLG00000007582
rosa_multiflora Rmu_sc0000744.1_g000050 Rmu_sc0003669.1_g000032
rosa_roxburghii Rroxscaffold_5G00365200
rosa_rugosa Rorug04G0184100
rosa_samantha Rh4AG244000 Rh4BG249100 Rh4CG258300 Rh4DG243600
rosa_wichuraiana Rw4G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 497
AccI GTMKAC 2 cut(s) 671, 1070
AccII CGCG 1 cut(s) 1061
AciI CCGC 3 cut(s) 1050, 1127, 1451
AclWI GGATC 3 cut(s) 270, 283, 1358
AcoI YGGCCR 2 cut(s) 515, 1389
AfaI GTAC 2 cut(s) 35, 1037
AfiI CCNNNNNNNGG 3 cut(s) 360, 1265, 1340
AgsI TTSAA 2 cut(s) 856, 1426
AhlI ACTAGT 2 cut(s) 731, 1150
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 496, 528
Alw21I GWGCWC 4 cut(s) 533, 1160, 1281, 1494
Alw26I GTCTC 2 cut(s) 867, 1190
Alw44I GTGCAC 2 cut(s) 529, 1490
AlwI GGATC 3 cut(s) 270, 283, 1358
Ama87I CYCGRG 1 cut(s) 1106
AoxI GGCC 6 cut(s) 515, 1099, 1113, 1331, 1389, 1602
ApaLI GTGCAC 2 cut(s) 529, 1490
ApeKI GCWGC 3 cut(s) 14, 98, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
AspLEI GCGC 3 cut(s) 498, 1061, 1384
AspS9I GGNCC 2 cut(s) 223, 1114
AsuHPI GGTGA 6 cut(s) 193, 467, 545, 556, 619, 810
AsuNHI GCTAGC 1 cut(s) 172
AvaI CYCGRG 1 cut(s) 1106
AvaII GGWCC 1 cut(s) 223
BaeGI GKGCMC 2 cut(s) 533, 1494
BalI TGGCCA 2 cut(s) 517, 1391
BamHI GGATCC 1 cut(s) 275
BanII GRGCYC 3 cut(s) 1160, 1281, 1535
BbsI GAAGAC 2 cut(s) 160, 461
Bbv12I GWGCWC 4 cut(s) 533, 1160, 1281, 1494
BbvI GCAGC 3 cut(s) 26, 110, 1111
BccI CCATC 5 cut(s) 402, 452, 1138, 1393, 1550
BciT130I CCWGG 1 cut(s) 1335
BclI TGATCA 1 cut(s) 1576
BcoDI GTCTC 2 cut(s) 867, 1190
BcuI ACTAGT 2 cut(s) 731, 1150
BfmI CTRYAG 1 cut(s) 259
BisI GCNGC 5 cut(s) 15, 99, 1050, 1125, 1452
BlpI GCTNAGC 1 cut(s) 235
BlsI GCNGC 5 cut(s) 16, 100, 1051, 1126, 1453
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 1 cut(s) 1106
BmgT120I GGNCC 2 cut(s) 223, 1114
BmiI GGNNCC 2 cut(s) 252, 277
BmrFI CCNGG 1 cut(s) 1335
BmsI GCATC 1 cut(s) 876
BmtI GCTAGC 1 cut(s) 176
BpiI GAAGAC 2 cut(s) 160, 461
Bpu10I CCTNAGC 2 cut(s) 1055, 1527
Bpu1102I GCTNAGC 1 cut(s) 235
BpuEI CTTGAG 2 cut(s) 1256, 1505
BsaAI YACGTR 1 cut(s) 1372
BsaBI GATNNNNATC 2 cut(s) 890, 1578
BsaJI CCNNGG 3 cut(s) 240, 291, 1245
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 1499, 1529
Bsc4I CCNNNNNNNGG 3 cut(s) 360, 1265, 1340
Bse118I RCCGGY 1 cut(s) 435
Bse3DI GCAATG 1 cut(s) 1040
Bse8I GATNNNNATC 2 cut(s) 890, 1578
BseBI CCWGG 1 cut(s) 1335
BseDI CCNNGG 3 cut(s) 240, 291, 1245
BseGI GGATG 5 cut(s) 78, 444, 891, 1149, 1561
BseJI GATNNNNATC 2 cut(s) 890, 1578
BseLI CCNNNNNNNGG 3 cut(s) 360, 1265, 1340
BseMI GCAATG 1 cut(s) 1040
BseRI GAGGAG 2 cut(s) 74, 195
BseSI GKGCMC 2 cut(s) 533, 1494
BseXI GCAGC 3 cut(s) 26, 110, 1111
BseYI CCCAGC 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 1061
BshFI GGCC 6 cut(s) 517, 1101, 1115, 1333, 1391, 1604
BsiHKAI GWGCWC 4 cut(s) 533, 1160, 1281, 1494
BsiHKCI CYCGRG 1 cut(s) 1106
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 1 cut(s) 1443
BslI CCNNNNNNNGG 3 cut(s) 360, 1265, 1340
BsmAI GTCTC 2 cut(s) 867, 1190
BsmBI CGTCTC 1 cut(s) 867
BsmFI GGGAC 1 cut(s) 1443
BsnI GGCC 6 cut(s) 517, 1101, 1115, 1333, 1391, 1604
BsoBI CYCGRG 1 cut(s) 1106
Bsp1286I GDGCHC 5 cut(s) 533, 1160, 1281, 1494, 1535
Bsp1407I TGTACA 1 cut(s) 1035
Bsp143I GATC 4 cut(s) 275, 451, 1350, 1576
Bsp1720I GCTNAGC 1 cut(s) 235
Bsp19I CCATGG 2 cut(s) 240, 291
BspACI CCGC 3 cut(s) 1050, 1127, 1451
BspANI GGCC 6 cut(s) 517, 1101, 1115, 1333, 1391, 1604
BspFNI CGCG 1 cut(s) 1061
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 2 cut(s) 252, 277
BspOI GCTAGC 1 cut(s) 176
BspPI GGATC 3 cut(s) 270, 283, 1358
BsrDI GCAATG 1 cut(s) 1040
BsrFI RCCGGY 1 cut(s) 435
BsrGI TGTACA 1 cut(s) 1035
BssAI RCCGGY 1 cut(s) 435
BssECI CCNNGG 3 cut(s) 240, 291, 1245
BssMI GATC 4 cut(s) 275, 451, 1350, 1576
BssNAI GTATAC 1 cut(s) 672
BssT1I CCWWGG 2 cut(s) 240, 291
Bst1107I GTATAC 1 cut(s) 672
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 3 cut(s) 837, 1246, 1486
Bst6I CTCTTC 4 cut(s) 348, 851, 1186, 1593
BstAUI TGTACA 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 1372
BstC8I GCNNGC 6 cut(s) 174, 424, 1047, 1099, 1277, 1281
BstDEI CTNAG 4 cut(s) 136, 235, 1055, 1527
BstDSI CCRYGG 3 cut(s) 240, 291, 1245
BstEII GGTNACC 1 cut(s) 968
BstF5I GGATG 5 cut(s) 78, 444, 891, 1149, 1561
BstFNI CGCG 1 cut(s) 1061
BstHHI GCGC 3 cut(s) 498, 1061, 1384
BstKTI GATC 4 cut(s) 278, 454, 1353, 1579
BstMAI GTCTC 2 cut(s) 867, 1190
BstMBI GATC 4 cut(s) 275, 451, 1350, 1576
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 2 cut(s) 1467, 1571
BstPI GGTNACC 1 cut(s) 968
BstSCI CCNGG 1 cut(s) 1333
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 2 cut(s) 533, 1494
BstUI CGCG 1 cut(s) 1061
BstV1I GCAGC 3 cut(s) 26, 110, 1111
BstV2I GAAGAC 2 cut(s) 160, 461
BstX2I RGATCY 1 cut(s) 275
BstXI CCANNNNNNTGG 5 cut(s) 109, 247, 272, 525, 1399
BstYI RGATCY 1 cut(s) 275
BstZ17I GTATAC 1 cut(s) 672
BsuRI GGCC 6 cut(s) 517, 1101, 1115, 1333, 1391, 1604
BtgI CCRYGG 3 cut(s) 240, 291, 1245
BtsCI GGATG 5 cut(s) 78, 444, 891, 1149, 1561
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 2 cut(s) 1143, 1491
Cac8I GCNNGC 6 cut(s) 174, 424, 1047, 1099, 1277, 1281
CciI TCATGA 1 cut(s) 951
CfoI GCGC 3 cut(s) 498, 1061, 1384
Cfr10I RCCGGY 1 cut(s) 435
Cfr13I GGNCC 2 cut(s) 223, 1114
CseI GACGC 2 cut(s) 1050, 1456
Csp6I GTAC 2 cut(s) 34, 1036
CspCI CAANNNNNGTGG 2 cut(s) 834, 869
CviQI GTAC 2 cut(s) 34, 1036
DdeI CTNAG 4 cut(s) 136, 235, 1055, 1527
DpnI GATC 4 cut(s) 277, 453, 1352, 1578
DpnII GATC 4 cut(s) 275, 451, 1350, 1576
EaeI YGGCCR 2 cut(s) 515, 1389
Eam1104I CTCTTC 4 cut(s) 348, 851, 1186, 1593
EarI CTCTTC 4 cut(s) 348, 851, 1186, 1593
Ecl136II GAGCTC 2 cut(s) 1158, 1279
Eco130I CCWWGG 2 cut(s) 240, 291
Eco147I AGGCCT 2 cut(s) 1333, 1604
Eco24I GRGCYC 3 cut(s) 1160, 1281, 1535
Eco47I GGWCC 1 cut(s) 223
Eco53kI GAGCTC 2 cut(s) 1158, 1279
Eco88I CYCGRG 1 cut(s) 1106
Eco91I GGTNACC 1 cut(s) 968
EcoICRI GAGCTC 2 cut(s) 1158, 1279
EcoO65I GGTNACC 1 cut(s) 968
EcoRII CCWGG 1 cut(s) 1333
EcoT14I CCWWGG 2 cut(s) 240, 291
EcoT22I ATGCAT 1 cut(s) 149
EcoT38I GRGCYC 3 cut(s) 1160, 1281, 1535
ErhI CCWWGG 2 cut(s) 240, 291
Esp3I CGTCTC 1 cut(s) 867
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FaqI GGGAC 1 cut(s) 1443
FbaI TGATCA 1 cut(s) 1576
FblI GTMKAC 2 cut(s) 671, 1070
Fnu4HI GCNGC 5 cut(s) 15, 99, 1050, 1125, 1452
FokI GGATG 5 cut(s) 85, 431, 898, 1156, 1568
FriOI GRGCYC 3 cut(s) 1160, 1281, 1535
Fsp4HI GCNGC 5 cut(s) 15, 99, 1050, 1125, 1452
FspI TGCGCA 1 cut(s) 497
GlaI GCGC 3 cut(s) 497, 1060, 1383
GluI GCNGC 5 cut(s) 15, 99, 1050, 1125, 1452
GsaI CCCAGC 1 cut(s) 706
HaeIII GGCC 6 cut(s) 517, 1101, 1115, 1333, 1391, 1604
HapII CCGG 1 cut(s) 436
HgaI GACGC 2 cut(s) 1050, 1456
HhaI GCGC 3 cut(s) 498, 1061, 1384
Hin6I GCGC 3 cut(s) 496, 1059, 1382
HinP1I GCGC 3 cut(s) 496, 1059, 1382
HindIII AAGCTT 1 cut(s) 424
HinfI GANTC 2 cut(s) 597, 1209
HpaII CCGG 1 cut(s) 436
HphI GGTGA 6 cut(s) 193, 467, 545, 556, 619, 810
Hpy166II GTNNAC 8 cut(s) 531, 672, 722, 863, 1038, 1071, 1292, 1492
Hpy188I TCNGA 6 cut(s) 399, 739, 799, 872, 896, 1597
Hpy188III TCNNGA 4 cut(s) 371, 574, 952, 1235
Hpy8I GTNNAC 8 cut(s) 531, 672, 722, 863, 1038, 1071, 1292, 1492
Hpy99I CGWCG 2 cut(s) 1066, 1472
HpyAV CCTTC 4 cut(s) 566, 589, 1105, 1545
HpyCH4III ACNGT 3 cut(s) 837, 1246, 1486
HpyCH4IV ACGT 2 cut(s) 691, 1371
HpyCH4V TGCA 9 cut(s) 8, 147, 310, 422, 531, 998, 1492, 1565, 1571
HpyF3I CTNAG 4 cut(s) 136, 235, 1055, 1527
HpySE526I ACGT 2 cut(s) 691, 1371
HspAI GCGC 3 cut(s) 496, 1059, 1382
Ksp22I TGATCA 1 cut(s) 1576
Kzo9I GATC 4 cut(s) 275, 451, 1350, 1576
LmnI GCTCC 3 cut(s) 61, 169, 182
Lsp1109I GCAGC 3 cut(s) 26, 110, 1111
LweI GCATC 1 cut(s) 876
MaeII ACGT 2 cut(s) 691, 1371
MaeIII GTNAC 8 cut(s) 327, 659, 706, 816, 825, 968, 1173, 1436
MalI GATC 4 cut(s) 277, 453, 1352, 1578
MboI GATC 4 cut(s) 275, 451, 1350, 1576
MboII GAAGA 9 cut(s) 165, 335, 461, 464, 542, 868, 1203, 1204, 1351
MfeI CAATTG 1 cut(s) 1285
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 5 cut(s) 533, 1160, 1281, 1494, 1535
MlsI TGGCCA 2 cut(s) 517, 1391
MluCI AATT 8 cut(s) 318, 417, 631, 793, 957, 1285, 1338, 1354
MluNI TGGCCA 2 cut(s) 517, 1391
MlyI GAGTC 1 cut(s) 606
Mox20I TGGCCA 2 cut(s) 517, 1391
Mph1103I ATGCAT 1 cut(s) 149
MscI TGGCCA 2 cut(s) 517, 1391
MslI CAYNNNNRTG 3 cut(s) 245, 1397, 1572
Msp20I TGGCCA 2 cut(s) 517, 1391
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 1335
MunI CAATTG 1 cut(s) 1285
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 1 cut(s) 1061
NcoI CCATGG 2 cut(s) 240, 291
NdeII GATC 4 cut(s) 275, 451, 1350, 1576
NheI GCTAGC 1 cut(s) 172
NlaIV GGNNCC 2 cut(s) 252, 277
NmuCI GTSAC 2 cut(s) 816, 1173
NsbI TGCGCA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 149
NspI RCATGY 2 cut(s) 1467, 1571
PagI TCATGA 1 cut(s) 951
PceI AGGCCT 2 cut(s) 1333, 1604
PcsI WCGNNNNNNNCGW 1 cut(s) 1272
PfeI GAWTC 1 cut(s) 1209
PkrI GCNGC 5 cut(s) 16, 100, 1051, 1126, 1453
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Ppu21I YACGTR 1 cut(s) 1372
Psp124BI GAGCTC 2 cut(s) 1160, 1281
Psp6I CCWGG 1 cut(s) 1333
PspEI GGTNACC 1 cut(s) 968
PspFI CCCAGC 1 cut(s) 702
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 2 cut(s) 252, 277
PspPI GGNCC 2 cut(s) 223, 1114
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 2 cut(s) 35, 1037
RsaNI GTAC 2 cut(s) 34, 1036
RseI CAYNNNNRTG 3 cut(s) 245, 1397, 1572
SacI GAGCTC 2 cut(s) 1160, 1281
SatI GCNGC 5 cut(s) 15, 99, 1050, 1125, 1452
Sau3AI GATC 4 cut(s) 275, 451, 1350, 1576
Sau96I GGNCC 2 cut(s) 223, 1114
SchI GAGTC 1 cut(s) 606
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 5 cut(s) 533, 1160, 1281, 1494, 1535
SfaNI GCATC 1 cut(s) 876
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 3 cut(s) 245, 1397, 1572
SmlI CTYRAG 2 cut(s) 1235, 1520
SmoI CTYRAG 2 cut(s) 1235, 1520
SpeI ACTAGT 2 cut(s) 731, 1150
Sse9I AATT 8 cut(s) 318, 417, 631, 793, 957, 1285, 1338, 1354
SseBI AGGCCT 2 cut(s) 1333, 1604
SsiI CCGC 3 cut(s) 1050, 1127, 1451
SstI GAGCTC 2 cut(s) 1160, 1281
StuI AGGCCT 2 cut(s) 1333, 1604
StyD4I CCNGG 1 cut(s) 1333
StyI CCWWGG 2 cut(s) 240, 291
TaaI ACNGT 3 cut(s) 837, 1246, 1486
TaiI ACGT 2 cut(s) 694, 1374
TaqI TCGA 3 cut(s) 372, 502, 1475
TaqII GACCGA 2 cut(s) 211, 1256
TasI AATT 8 cut(s) 318, 417, 631, 793, 957, 1285, 1338, 1354
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 2 cut(s) 1052, 1454
TfiI GAWTC 1 cut(s) 1209
TscAI CASTG 2 cut(s) 1143, 1491
TseFI GTSAC 2 cut(s) 816, 1173
TseI GCWGC 3 cut(s) 14, 98, 1124
Tsp45I GTSAC 2 cut(s) 816, 1173
TspDTI ATGAA 2 cut(s) 840, 940
TspGWI ACGGA 2 cut(s) 725, 891
TspRI CASTG 2 cut(s) 1143, 1491
VneI GTGCAC 2 cut(s) 529, 1490
VpaK11BI GGWCC 1 cut(s) 223
XceI RCATGY 2 cut(s) 1467, 1571
XmiI GTMKAC 2 cut(s) 671, 1070
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.