Rorug04G0184100

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014383
Physical Location & Seq
Reverse (-)
32111039 .. 32119220
8182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0184100.1

Sequence Viewer

Length: 2319 bp
ATGTCGGAGATGGCGAACACCGACCCTGAGGGGATCGACGGAGTCCGCATGACGTGGAACGTCTGGCCCCGCACAAAAGTCGAGGCCAGCAAGTGCGTGATCCCACTCGCCGCCTGCATCGCCCCGATCCGCTTCCACACCGACATCCCCACGCTCCCCTACGCGCCTCTCCGCTGCAAGACCTGCTCCGCCGTCCTCAACGCCTTCTCGCGCGTCGACTTCAACGCCAAGATCTGGATCTGCCCTTTCTGCTACCAGCGCAACCACTTCCCTCCCCACTACTCTATGATCTCCGAGACCAACCTCCCCGGCGAGCTCTACCCTCAGTACACCACCGTCCAGTACTCCCTTCCCCCCGATTCGACGCCGCACTCGCTGCCGGCGCCGGTCTTCGTCTTCGTGCTCGACACGTGTATGATCGAGGAGGAGATGGGGTATGTCAAATCGGCGCTGAAGCAGGCGATTGGGCTGCTGCCGGAGAATGCTTTGGTAGGGTTCATCTCGTTCGGGACTCAGGTGCAGGTGCATGAATTGGGGTTCCCCGAATTGAGCAAGGTCTATGTGTTTAGGGGGACTAAGGAGATTTCTAAAGATCAGATTCTGGAGCAATTGGGCCTGGTTAGTTCCGGCCGGAGGCCCGGTCCTGTTGGAGGAGGGTACAACCAGCCAAAGGGGCTGCAGAATGGGTTTCCTAATTCGGGGGTCACCCGATTTCTCTTGCCCGCTTCGGAATGCGAATACACGCTCGATGCGTTGTTGGATGAATTGCAAAGCGACCAGTGGCCTGTGCCGCCTGGTAATAGGTCGTCAAGGTGTACCGGAGTGGCCGTGAGTGTTGCAGCCGGTTTGCTTGGGGCTTGTGTGGCTGGAACTGGGGCACGGATTGTAGCCTTGGTTGGTGGTCCGTGTACTGAAGGGCCAGGAGCGATTGTTTCAAAAGATCTATCAGAACCAGTACGTTCCCATAAAGATCTGGATAAGGATGCAGCCCCATACTTTAAGAAAGCAGTCAAGTTTTATGATAGTCTTGCGAAGCAGCTAGTTAGCCAGGGTCATGTCTTAGACCTCTTCGCATCTGCACTTGATCAGGTTGGAGTTGCAGAAATGAAAGTTGCAATTGAAAAGACAGGTGGACTTGTTGTTCTGGCAGAAAGTTTTGGTCATTCTGTATTCAAAGATTCTTTCAGGCGTGTCTTTGAACAAGGGGAGCAGTCTCTTGGCCTTTGTTTTAATGGCACCCTTGAGATAAACTGTTCAAAGGACATCAAAATTCAGGGGGTCATTGGACCTTGCACGTCATTGGAGAAGAAAGGACCTGCTGTTGCCGATACTTCCATAGGAGAAGGGAATACAACAGCGTGGAAAATGTGTAGCCTTGACAGGAGTACTTGCTTGACAGTTTTCTTTGATTTATCATCAAGTGATCAGTCAAATACACCAGGAACAGTAAACCCACAATTGTATTTGCAGTTCCTTACAAGTTTTCAAAACCCTCAAGGTCAAATGATGCTTCGAGTAACAACAATTACTAGGCGATGGATAGACAGTTCTGTTAGCTCAGAGGAATTGGTTCAAGGATTTGACCAAGAGACTGCTGCTGTAGTAATGGCAAGAAAAACGTCTTTGAAAATGGAGACGGAGGAGTCATTTGATGCTACCCGATGGCTGGATCGGGGACTTATTCGTCTCTGTTCCAAATTTGGTGACTATCGGAAAGATGATCCAGCATCCTTTACTTTAAATCCATGTTTTTCATTATTCCCTCAATTTATGTTCAATCTCCGGCGCTCGCAATTTGTACAGGTTTTCAACAACAGTCCAGATGAAACTGCCTATTTTCGCATGCTGTTGAATCGGGAAAATATCACCAATGCTGCTGTCATGATTCAACCATCATTAATATCATACTCATTTAATTCTCTACCTCAGCCAGCATTGTTGGATGTTGCTTCCATTGCAGCTGACCGTATTCTGTTGTTGGATTCATATTTTAGTATAGTCATTTTCCATGGAATGACAATAGCACAGTGGCGTAACATGGGATACCAGAATCAGCCAGAACACCTGGCATTTGCACAATTATTGCAAGCTCCTCATGATGATACACAGATGATCATTCGTGAACGTTTCCCTGTCCCTAGGTTGGTGGTTTGCGATCAGCATGGTTCCCAGGCAAGGTTCTTATTAGCAAAGTTGAACCCTTCGGCAACATACAACAATGCACATGAGATGGCTGCTGGATCAGATGTGATCTTCACAGATGATGTAAGTCTTCAGGTCTTTTTCGAGCATCTTCAGAGGCTGGCAGTGCAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0006810 GO:0006855 GO:0008150 GO:0015238 GO:0015893 GO:0022857 GO:0042221 GO:0042493 GO:0050896 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

772

Amino Acids

85.24

Weight (kDa)

5.7

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 56 - 94 5.5e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 128 - 399 3.2e-66 Sec23/Sec24 trunk domain
Sec23_BS PF08033 410 - 513 1e-23 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 528 - 625 9.9e-26 Sec23/Sec24 helical domain
Gelsolin PF00626 640 - 728 1.4e-14 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49130
fragaria_vesca FvH4_4g18550
malus_domestica MD13G1166100.v1.1 MD16G1165600.v1.1
prunus_persica Prupe.1G132600_v2.0.a1
pyrus_communis pycom16g14030
rosa_chinensis RchiOBHm_Chr4g0423121
rosa_laevigata RLG00000007582
rosa_multiflora Rmu_sc0000744.1_g000050 Rmu_sc0003669.1_g000032
rosa_roxburghii Rroxscaffold_5G00365200
rosa_rugosa Rorug04G0184100
rosa_samantha Rh4AG244000 Rh4BG249100 Rh4CG258300 Rh4DG243600
rosa_wichuraiana Rw4G021030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
AasI GACNNNNNNGTC 2 cut(s) 1642, 1683
Acc36I ACCTGC 3 cut(s) 191, 511, 1324
AccB1I GGYRCC 2 cut(s) 382, 1235
AccB7I CCANNNNNTGG 1 cut(s) 234
AccI GTMKAC 1 cut(s) 216
AccII CGCG 3 cut(s) 164, 211, 213
AciI CCGC 9 cut(s) 46, 70, 111, 130, 172, 189, 368, 723, 791
AclI AACGTT 1 cut(s) 2125
AclWI GGATC 7 cut(s) 41, 94, 121, 245, 1677, 1715, 2248
AcoI YGGCCR 2 cut(s) 628, 825
AcsI RAATTY 2 cut(s) 1269, 1697
AcuI CTGAAG 4 cut(s) 473, 933, 2258, 2279
AcvI CACGTG 1 cut(s) 411
AcyI GRCGYC 2 cut(s) 365, 383
AfaI GTAC 8 cut(s) 329, 344, 659, 817, 910, 957, 1387, 1800
AfiI CCNNNNNNNGG 8 cut(s) 234, 633, 650, 670, 698, 1666, 2143, 2175
AflIII ACRYGT 2 cut(s) 408, 410
AjiI CACGTC 2 cut(s) 54, 1296
AjnI CCWGG 7 cut(s) 615, 793, 919, 1047, 1438, 2064, 2169
AloI GAACNNNNNNTCC 4 cut(s) 1125, 1157, 1531, 1563
AluBI AGCT 5 cut(s) 316, 1039, 1557, 1961, 2090
AluI AGCT 5 cut(s) 316, 1039, 1557, 1961, 2090
Alw21I GWGCWC 2 cut(s) 318, 405
Alw26I GTCTC 5 cut(s) 290, 1218, 1583, 1628, 1691
AlwI GGATC 7 cut(s) 41, 94, 121, 245, 1677, 1715, 2248
AlwNI CAGNNNCTG 2 cut(s) 601, 2302
AoxI GGCC 9 cut(s) 65, 84, 613, 628, 635, 782, 825, 917, 1219
ApoI RAATTY 2 cut(s) 1269, 1697
AseI ATTAAT 1 cut(s) 1898
Asp700I GAANNNNTTC 1 cut(s) 1569
AspA2I CCTAGG 1 cut(s) 2138
AspLEI GCGC 6 cut(s) 166, 213, 261, 385, 451, 1788
AspS9I GGNCC 8 cut(s) 66, 613, 636, 641, 902, 917, 1286, 1313
AsuC2I CCSGG 2 cut(s) 309, 639
AsuHPI GGTGA 3 cut(s) 697, 1715, 1858
AvaII GGWCC 4 cut(s) 641, 902, 1286, 1313
AvrII CCTAGG 1 cut(s) 2138
AxyI CCTNAGG 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 880
BanI GGYRCC 2 cut(s) 382, 1235
BanII GRGCYC 1 cut(s) 318
BbrPI CACGTG 1 cut(s) 411
BbsI GAAGAC 3 cut(s) 382, 388, 2264
Bbv12I GWGCWC 2 cut(s) 318, 405
BbvCI CCTCAGC 1 cut(s) 1926
BccI CCATC 6 cut(s) 4, 424, 1530, 1656, 1900, 2224
BceAI ACGGC 2 cut(s) 176, 812
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 451, 485
BciT130I CCWGG 7 cut(s) 617, 795, 921, 1049, 1440, 2066, 2171
BciVI GTATCC 1 cut(s) 2036
BclI TGATCA 3 cut(s) 1084, 1423, 2112
BcnI CCSGG 2 cut(s) 309, 639
BcoDI GTCTC 5 cut(s) 290, 1218, 1583, 1628, 1691
BfaI CTAG 3 cut(s) 1040, 1530, 2139
BfmI CTRYAG 2 cut(s) 677, 1599
BfoI RGCGCY 3 cut(s) 386, 452, 1789
BfuAI ACCTGC 3 cut(s) 191, 511, 1324
BfuI GTATCC 1 cut(s) 2036
BglI GCCNNNNNGGC 1 cut(s) 673
BglII AGATCT 3 cut(s) 231, 940, 970
BlnI CCTAGG 1 cut(s) 2138
BmcAI AGTACT 2 cut(s) 344, 1387
Bme1390I CCNGG 9 cut(s) 309, 617, 639, 795, 921, 1049, 1440, 2066, 2171
Bme18I GGWCC 4 cut(s) 641, 902, 1286, 1313
BmgBI CACGTC 2 cut(s) 54, 1296
BmgT120I GGNCC 8 cut(s) 66, 613, 636, 641, 902, 917, 1286, 1313
BmiI GGNNCC 5 cut(s) 68, 384, 539, 1237, 2167
BmrFI CCNGG 9 cut(s) 309, 617, 639, 795, 921, 1049, 1440, 2066, 2171
BmrI ACTGGG 1 cut(s) 882
BmsI GCATC 8 cut(s) 126, 739, 973, 1082, 1497, 1642, 1736, 2299
BmuI ACTGGG 1 cut(s) 882
BpiI GAAGAC 3 cut(s) 382, 388, 2264
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 1926
BpuEI CTTGAG 2 cut(s) 1262, 1479
BpuMI CCSGG 2 cut(s) 309, 639
BsaAI YACGTR 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 365, 383
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 6 cut(s) 307, 891, 1048, 2008, 2138, 2169
BsaWI WCCGGW 1 cut(s) 818
BsaXI ACNNNNNCTCC 8 cut(s) 419, 449, 625, 655, 813, 843, 915, 945
Bsc4I CCNNNNNNNGG 8 cut(s) 234, 633, 650, 670, 698, 1666, 2143, 2175
Bse118I RCCGGY 3 cut(s) 379, 385, 842
Bse1I ACTGG 4 cut(s) 340, 778, 877, 953
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 1953
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 7 cut(s) 617, 795, 921, 1049, 1440, 2066, 2171
BseDI CCNNGG 6 cut(s) 307, 891, 1048, 2008, 2138, 2169
BseGI GGATG 5 cut(s) 144, 766, 988, 1727, 1948
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 8 cut(s) 234, 633, 650, 670, 698, 1666, 2143, 2175
BseMI GCAATG 1 cut(s) 1953
BseMII CTCAG 5 cut(s) 18, 338, 527, 1572, 1940
BseNI ACTGG 4 cut(s) 340, 778, 877, 953
BseRI GAGGAG 5 cut(s) 437, 440, 666, 1655, 2082
BseSI GKGCMC 1 cut(s) 880
BseX3I CGGCCG 1 cut(s) 628
BsgI GTGCAG 2 cut(s) 539, 1062
Bsh1236I CGCG 3 cut(s) 164, 211, 213
Bsh1285I CGRYCG 1 cut(s) 631
BshFI GGCC 9 cut(s) 67, 86, 615, 630, 637, 784, 827, 919, 1221
BshNI GGYRCC 2 cut(s) 382, 1235
BsiEI CGRYCG 1 cut(s) 631
BsiHKAI GWGCWC 2 cut(s) 318, 405
BslFI GGGAC 4 cut(s) 523, 586, 1689, 2120
BslI CCNNNNNNNGG 8 cut(s) 234, 633, 650, 670, 698, 1666, 2143, 2175
BsmAI GTCTC 5 cut(s) 290, 1218, 1583, 1628, 1691
BsmBI CGTCTC 2 cut(s) 1628, 1691
BsmFI GGGAC 4 cut(s) 523, 586, 1689, 2120
BsmI GAATGC 2 cut(s) 487, 737
BsnI GGCC 9 cut(s) 67, 86, 615, 630, 637, 784, 827, 919, 1221
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 3 cut(s) 318, 405, 880
Bsp1407I TGTACA 1 cut(s) 1798
Bsp19I CCATGG 1 cut(s) 2008
BspACI CCGC 9 cut(s) 46, 70, 111, 130, 172, 189, 368, 723, 791
BspANI GGCC 9 cut(s) 67, 86, 615, 630, 637, 784, 827, 919, 1221
BspCNI CTCAG 5 cut(s) 19, 337, 526, 1571, 1939
BspFNI CGCG 3 cut(s) 164, 211, 213
BspHI TCATGA 2 cut(s) 1881, 2095
BspLI GGNNCC 5 cut(s) 68, 384, 539, 1237, 2167
BspMAI CTGCAG 1 cut(s) 681
BspMI ACCTGC 3 cut(s) 191, 511, 1324
BspPI GGATC 7 cut(s) 41, 94, 121, 245, 1677, 1715, 2248
BspT107I GGYRCC 2 cut(s) 382, 1235
BspTNI GGTCTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 1953
BsrFI RCCGGY 3 cut(s) 379, 385, 842
BsrGI TGTACA 1 cut(s) 1798
BsrI ACTGG 4 cut(s) 340, 778, 877, 953
BssAI RCCGGY 3 cut(s) 379, 385, 842
BssECI CCNNGG 6 cut(s) 307, 891, 1048, 2008, 2138, 2169
BssNI GRCGYC 2 cut(s) 365, 383
BssT1I CCWWGG 3 cut(s) 891, 2008, 2138
Bst2UI CCWGG 7 cut(s) 617, 795, 921, 1049, 1440, 2066, 2171
Bst4CI ACNGT 8 cut(s) 337, 1253, 1399, 1447, 1547, 1817, 1967, 2028
Bst6I CTCTTC 1 cut(s) 1073
BstACI GRCGYC 2 cut(s) 365, 383
BstAPI GCANNNNNTGC 2 cut(s) 183, 376
BstAUI TGTACA 1 cut(s) 1798
BstBAI YACGTR 1 cut(s) 411
BstDEI CTNAG 8 cut(s) 27, 324, 513, 576, 1060, 1558, 1926, 2316
BstDSI CCRYGG 1 cut(s) 2008
BstEII GGTNACC 1 cut(s) 703
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 5 cut(s) 144, 766, 988, 1727, 1948
BstFNI CGCG 3 cut(s) 164, 211, 213
BstH2I RGCGCY 3 cut(s) 386, 452, 1789
BstHHI GCGC 6 cut(s) 166, 213, 261, 385, 451, 1788
BstMAI GTCTC 5 cut(s) 290, 1218, 1583, 1628, 1691
BstMCI CGRYCG 1 cut(s) 631
BstNI CCWGG 7 cut(s) 617, 795, 921, 1049, 1440, 2066, 2171
BstNSI RCATGY 1 cut(s) 1846
BstPI GGTNACC 1 cut(s) 703
BstSCI CCNGG 9 cut(s) 307, 615, 637, 793, 919, 1047, 1438, 2064, 2169
BstSFI CTRYAG 2 cut(s) 677, 1599
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 3 cut(s) 164, 211, 213
BstV2I GAAGAC 3 cut(s) 382, 388, 2264
BstX2I RGATCY 4 cut(s) 231, 237, 940, 970
BstYI RGATCY 4 cut(s) 231, 237, 940, 970
BstZI CGGCCG 1 cut(s) 628
Bsu36I CCTNAGG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 2036
BsuRI GGCC 9 cut(s) 67, 86, 615, 630, 637, 784, 827, 919, 1221
BtgI CCRYGG 1 cut(s) 2008
BtgZI GCGATG 2 cut(s) 103, 1549
BtrI CACGTC 2 cut(s) 54, 1296
BtsCI GGATG 5 cut(s) 144, 766, 988, 1727, 1948
BtsI GCAGTG 1 cut(s) 2313
BtsIMutI CAGTG 3 cut(s) 785, 2033, 2313
BveI ACCTGC 3 cut(s) 191, 511, 1324
CaiI CAGNNNCTG 2 cut(s) 601, 2302
CciI TCATGA 2 cut(s) 1881, 2095
CfoI GCGC 6 cut(s) 166, 213, 261, 385, 451, 1788
Cfr10I RCCGGY 3 cut(s) 379, 385, 842
Cfr13I GGNCC 8 cut(s) 66, 613, 636, 641, 902, 917, 1286, 1313
CseI GACGC 2 cut(s) 202, 373
Csp6I GTAC 8 cut(s) 328, 343, 658, 816, 909, 956, 1386, 1799
CviQI GTAC 8 cut(s) 328, 343, 658, 816, 909, 956, 1386, 1799
DdeI CTNAG 8 cut(s) 27, 324, 513, 576, 1060, 1558, 1926, 2316
DinI GGCGCC 1 cut(s) 384
DraI TTTAAA 1 cut(s) 1740
DrdI GACNNNNNNGTC 2 cut(s) 1642, 1683
DseDI GACNNNNNNGTC 2 cut(s) 1642, 1683
EaeI YGGCCR 2 cut(s) 628, 825
EagI CGGCCG 1 cut(s) 628
Eam1104I CTCTTC 1 cut(s) 1073
EarI CTCTTC 1 cut(s) 1073
EciI GGCGGA 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 891, 2008, 2138
Eco24I GRGCYC 1 cut(s) 318
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 4 cut(s) 641, 902, 1286, 1313
Eco52I CGGCCG 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 4 cut(s) 473, 933, 2258, 2279
Eco72I CACGTG 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 27
Eco91I GGTNACC 1 cut(s) 703
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 1313
EcoO65I GGTNACC 1 cut(s) 703
EcoRII CCWGG 7 cut(s) 615, 793, 919, 1047, 1438, 2064, 2169
EcoT14I CCWWGG 3 cut(s) 891, 2008, 2138
EcoT38I GRGCYC 1 cut(s) 318
EgeI GGCGCC 1 cut(s) 384
EheI GGCGCC 1 cut(s) 384
ErhI CCWWGG 3 cut(s) 891, 2008, 2138
Esp3I CGTCTC 2 cut(s) 1628, 1691
FaqI GGGAC 4 cut(s) 523, 586, 1689, 2120
FauI CCCGC 2 cut(s) 77, 730
FbaI TGATCA 3 cut(s) 1084, 1423, 2112
FblI GTMKAC 1 cut(s) 216
FokI GGATG 5 cut(s) 131, 773, 995, 1714, 1955
FriOI GRGCYC 1 cut(s) 318
FspBI CTAG 3 cut(s) 1040, 1530, 2139
GlaI GCGC 6 cut(s) 165, 212, 260, 384, 450, 1787
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 3 cut(s) 386, 452, 1789
HaeIII GGCC 9 cut(s) 67, 86, 615, 630, 637, 784, 827, 919, 1221
HgaI GACGC 2 cut(s) 202, 373
HhaI GCGC 6 cut(s) 166, 213, 261, 385, 451, 1788
Hin1I GRCGYC 2 cut(s) 365, 383
Hin6I GCGC 6 cut(s) 164, 211, 259, 383, 449, 1786
HinP1I GCGC 6 cut(s) 164, 211, 259, 383, 449, 1786
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HphI GGTGA 3 cut(s) 697, 1715, 1858
Hpy166II GTNNAC 7 cut(s) 217, 330, 816, 909, 1133, 1450, 2123
Hpy188I TCNGA 9 cut(s) 7, 295, 597, 730, 949, 1561, 1713, 2245, 2298
Hpy188III TCNNGA 9 cut(s) 235, 508, 602, 974, 1820, 1856, 1882, 2096, 2120
Hpy8I GTNNAC 7 cut(s) 217, 330, 816, 909, 1133, 1450, 2123
Hpy99I CGWCG 3 cut(s) 41, 218, 367
HpyAV CCTTC 5 cut(s) 214, 359, 908, 1337, 2211
HpyCH4III ACNGT 8 cut(s) 337, 1253, 1399, 1447, 1547, 1817, 1967, 2028
HpyCH4IV ACGT 7 cut(s) 53, 60, 410, 958, 1295, 1619, 2125
HpyF3I CTNAG 8 cut(s) 27, 324, 513, 576, 1060, 1558, 1926, 2316
HpySE526I ACGT 7 cut(s) 53, 60, 410, 958, 1295, 1619, 2125
Hsp92I GRCGYC 2 cut(s) 365, 383
HspAI GCGC 6 cut(s) 164, 211, 259, 383, 449, 1786
KasI GGCGCC 1 cut(s) 382
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 3 cut(s) 1084, 1423, 2112
LmnI GCTCC 6 cut(s) 159, 191, 604, 923, 1207, 2095
LweI GCATC 8 cut(s) 126, 739, 973, 1082, 1497, 1642, 1736, 2299
MaeI CTAG 3 cut(s) 1040, 1530, 2139
MaeII ACGT 7 cut(s) 53, 60, 410, 958, 1295, 1619, 2125
MaeIII GTNAC 4 cut(s) 703, 1516, 1703, 2033
MboII GAAGA 7 cut(s) 382, 388, 1060, 1318, 2245, 2264, 2285
MfeI CAATTG 3 cut(s) 608, 1116, 1457
MflI RGATCY 4 cut(s) 231, 237, 940, 970
MhlI GDGCHC 3 cut(s) 318, 405, 880
Mly113I GGCGCC 1 cut(s) 383
MlyI GAGTC 3 cut(s) 51, 505, 1652
MmeI TCCRAC 5 cut(s) 628, 738, 1072, 1920, 1959
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 1 cut(s) 1569
MseI TTAA 5 cut(s) 999, 1230, 1739, 1898, 1914
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 2 cut(s) 174, 1961
MspR9I CCNGG 9 cut(s) 309, 617, 639, 795, 921, 1049, 1440, 2066, 2171
MunI CAATTG 3 cut(s) 608, 1116, 1457
Mva1269I GAATGC 2 cut(s) 487, 737
MvaI CCWGG 7 cut(s) 617, 795, 921, 1049, 1440, 2066, 2171
MvnI CGCG 3 cut(s) 164, 211, 213
NaeI GCCGGC 1 cut(s) 381
NarI GGCGCC 1 cut(s) 383
NciI CCSGG 2 cut(s) 309, 639
NcoI CCATGG 1 cut(s) 2008
NgoMIV GCCGGC 1 cut(s) 379
NlaIV GGNNCC 5 cut(s) 68, 384, 539, 1237, 2167
NmuCI GTSAC 2 cut(s) 703, 1703
NspI RCATGY 1 cut(s) 1846
PaeI GCATGC 1 cut(s) 1846
PagI TCATGA 2 cut(s) 1881, 2095
PaqCI CACCTGC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 749
PctI GAATGC 2 cut(s) 487, 737
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 1569
PfeI GAWTC 7 cut(s) 359, 598, 1178, 1852, 1885, 1982, 2050
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 234
PleI GAGTC 3 cut(s) 50, 505, 1651
PluTI GGCGCC 1 cut(s) 386
PmaCI CACGTG 1 cut(s) 411
PmlI CACGTG 1 cut(s) 411
PpsI GAGTC 3 cut(s) 50, 505, 1651
Ppu21I YACGTR 1 cut(s) 411
PpuMI RGGWCCY 1 cut(s) 1313
PshBI ATTAAT 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 318
Psp1406I AACGTT 1 cut(s) 2125
Psp5II RGGWCCY 1 cut(s) 1313
Psp6I CCWGG 7 cut(s) 615, 793, 919, 1047, 1438, 2064, 2169
PspCI CACGTG 1 cut(s) 411
PspEI GGTNACC 1 cut(s) 703
PspGI CCWGG 7 cut(s) 615, 793, 919, 1047, 1438, 2064, 2169
PspN4I GGNNCC 5 cut(s) 68, 384, 539, 1237, 2167
PspPI GGNCC 8 cut(s) 66, 613, 636, 641, 902, 917, 1286, 1313
PspPPI RGGWCCY 1 cut(s) 1313
PsrI GAACNNNNNNTAC 2 cut(s) 1454, 1486
PstI CTGCAG 1 cut(s) 681
PstNI CAGNNNCTG 2 cut(s) 601, 2302
PsuI RGATCY 4 cut(s) 231, 237, 940, 970
PsyI GACNNNGTC 1 cut(s) 41
PvuII CAGCTG 1 cut(s) 1961
RsaI GTAC 8 cut(s) 329, 344, 659, 817, 910, 957, 1387, 1800
RsaNI GTAC 8 cut(s) 328, 343, 658, 816, 909, 956, 1386, 1799
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 5 cut(s) 999, 1230, 1739, 1898, 1914
Sau96I GGNCC 8 cut(s) 66, 613, 636, 641, 902, 917, 1286, 1313
ScaI AGTACT 2 cut(s) 344, 1387
SchI GAGTC 3 cut(s) 51, 505, 1652
ScrFI CCNGG 9 cut(s) 309, 617, 639, 795, 921, 1049, 1440, 2066, 2171
SduI GDGCHC 3 cut(s) 318, 405, 880
SfaNI GCATC 8 cut(s) 126, 739, 973, 1082, 1497, 1642, 1736, 2299
SfcI CTRYAG 2 cut(s) 677, 1599
SfoI GGCGCC 1 cut(s) 384
SinI GGWCC 4 cut(s) 641, 902, 1286, 1313
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 1241, 1494
SmoI CTYRAG 2 cut(s) 1241, 1494
SphI GCATGC 1 cut(s) 1846
SsiI CCGC 9 cut(s) 46, 70, 111, 130, 172, 189, 368, 723, 791
SspDI GGCGCC 1 cut(s) 382
SspMI CTAG 3 cut(s) 1040, 1530, 2139
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 9 cut(s) 307, 615, 637, 793, 919, 1047, 1438, 2064, 2169
StyI CCWWGG 3 cut(s) 891, 2008, 2138
TaaI ACNGT 8 cut(s) 337, 1253, 1399, 1447, 1547, 1817, 1967, 2028
TaiI ACGT 7 cut(s) 56, 63, 413, 961, 1298, 1622, 2128
TaqI TCGA 9 cut(s) 36, 81, 216, 362, 405, 420, 747, 1513, 2286
TatI WGTACW 5 cut(s) 327, 342, 908, 1385, 1798
TauI GCSGC 3 cut(s) 113, 370, 793
TfiI GAWTC 7 cut(s) 359, 598, 1178, 1852, 1885, 1982, 2050
Tru1I TTAA 5 cut(s) 999, 1230, 1739, 1898, 1914
Tru9I TTAA 5 cut(s) 999, 1230, 1739, 1898, 1914
TscAI CASTG 3 cut(s) 785, 2033, 2313
TseFI GTSAC 2 cut(s) 703, 1703
Tsp45I GTSAC 2 cut(s) 703, 1703
TspDTI ATGAA 7 cut(s) 487, 543, 777, 1121, 1743, 1839, 1974
TspGWI ACGGA 4 cut(s) 54, 894, 895, 1652
TspRI CASTG 3 cut(s) 785, 2033, 2313
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 234
VpaK11BI GGWCC 4 cut(s) 641, 902, 1286, 1313
VspI ATTAAT 1 cut(s) 1898
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 2 cut(s) 1269, 1697
XceI RCATGY 1 cut(s) 1846
XmaJI CCTAGG 1 cut(s) 2138
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 1569
XspI CTAG 3 cut(s) 1040, 1530, 2139
ZrmI AGTACT 2 cut(s) 344, 1387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.