MD13G1216700.v1.1

Long-chain fatty alcohol oxidase involved in the omega- oxidation pathway of lipid degradation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010757
Physical Location & Seq
Forward (+)
20707140 .. 20712396
5257 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1216700.v1.1.491

Sequence Viewer

Length: 2184 bp
ATGAGAAAGGTCTGTCATCCTTTGTTGAATGGGGGAAGAGGATATGCAGGAGATGACAGAAAATACAGTCATGGATTCTCTTCAGCTGAGATGCAGTCTTTGGCAAGTATCTGTGAGGCTTTATTACCTCCTTTTTATGAAACCAAAGGTGAACAACCGACAATTAACAAGGCTGTTCAGTCCTTCTACAAAACTTCTGCGGCTCAAACTCCCATCCCTGATGAGGTTGCACAGCTAATGGTGAAAAGGGCCTTGATAGAAGCTGTGGTATTGGTTAGAGTGGTTTTGTGGATATTGGGAACTAAGTTGGGAACCCTACTCCTCTGTGGCTCTCTTTGTTTTGGTAAGACATGGCCCTTCGTTCACAAGTTTTCAAGTTTGTCGTTGGAGAAAAGAGAAAAGGTCCTGCAGAAGTGGTTCAAGCACAAGTTTCTTACCCCCATTAGGCTTGCCTTTGTTTACATCAAGTTTCTTTGCCTCTACATTTTCTTCTCTCGGGTTGGTGAGAATGCAGAAAACCCAATATGGCAAGCCATTGGATACCATGTAGAAACTGATGACCAAGATGATCAGAAGAACCACTCAAAAATGCAAGAACAAAAGCGGCCTCTTCAGAAGGGAATCATAGAAACAAGGCTTGAAACTGATACCAGCCTTCTGAATTCCCTTGAACAGAAAGGCTTCCAAGTCATACATAACTCCACAAAAAATATTTACAACGTCAAATGTGACGTTATAATTGTTGGCTCTGGTTGTGGAGGAGGTGTCGCAGCTGCTGCTCTTGCAAGCTCAGGCCACAAAGTCATCGTTCTCGAAAAAGGAAACTACTTCACTCCCTCAGATTACTCTTCTTTGGAAGCTCCCTCCCATGATCACCTTTATGAATCAGGAGGCATTCTTGTAACTGTTGATGGGAAAATTGTGCTTCAGGCTGGATCAACGGTTGGTGGTGGCTCTGCTATTAATTGGTCAGCCTGTATTAAAACACCAAATTATGTGCTTCAGGAATGGAATAAAGATCATAAGATCAAGTTCTTCGGGGGATCGGAGTATCTTTCTGCAATGGATACTGTATGCGAGAGAATTGGTGTTACTGAGAATTGTGTAGAAGAAGGGTTTCAAAATCAAGTACTAAGGAAAGGGTGTGAGAATCTAGGCCTTGGGAAAGGAGAGAAAAAGGGGACAGATTCTACATGGCTAATGGATGCAGTGGATTATGGTGCCGTGATCATAACTGGATGTAAAGCTGAGAGATTTGTATTGGAAACCAACAAGAGTGAAAGTAAAAGAAAAAAGAAATGTTTGGGAGTAATGGCAAAACCTTTGAGCAATAACATTACGAAGAGGCTGCAAATTGAGGCCAAAGTTACAATCTCAGCTTGTGGAGCTCTTTTGACACCCCCTTTAATGCTTTCTAGTGGACTGAAGAACAAGAACATTGGTCGGAATCTTCATCTCCACCCTGTTTTAATGGCTTGGGGATATTTCCCTGACTCAAATTCAGAGTTCAAGGGTAAAAACTATGAGGGTGGAATAATTACATCAGTGTATAAGGTGGTATCAGCAGACTCTAAGGTAAAAGCAATCATAGAAACACCGGCATTAGGGCCGGGAACATTTTCTGCTTTGAGCCCTTGGGTGTCTGGGGAAGACATAAAGAACAGAATGCTGAAGTTTTCAAGAACTGCCCATTTGATTTCAATTATCAGGGATAAGGGTTCCGGGGTAGTTACGAAGGGTGGAAGAGTAAGTTATGAGTTTTCTGCCTTAGACAAAGAGAACATAAAGGGAGGTTTGAGGCAGGCATTAAGGATTCTAATAGCAGCAGGAGCAATTGAAGTTGGCACACATCGGAGTGATGGGCTGAGACTCAAATGTAAAGGGATTGATAAGGAGGAACTGGAGGAGTTTCTTGACATGGTTACTGCAGATGAAGGGCCGCAGTCACTGGTAGAGAATTGGACAATCTATTCCTCAGCTCATCAGATGGGGAGCTGTAGAATGGGAATCAATGCAAAGGAAGGGGCGGTTGATGAGAATGGGGAGAGCTGGGAAGCAGAAAGCCTCTTCGTATGTGATGGGAGTGTTCTACCTAGTGCTGTTGGTGTTAATCCCATGATCACCATCCAATCAACTGCATACTGTCTCTCAAAGAGAATAGCTGATTCTCTAAGAAAGTGTTAG

Protein Analysis

728

Amino Acids

79.7

Weight (kDa)

8.69

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_2 PF00890 244 - 277 2.5e-06 FAD binding domain
GMC_oxred_N PF00732 291 - 387 3.6e-31 GMC oxidoreductase
GMC_oxred_N PF00732 389 - 489 4.8e-28 GMC oxidoreductase
GMC_oxred_C PF05199 585 - 713 6.3e-20 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23410 AT3G23410
fragaria_vesca FvH4_4g04030 FvH4_4g04030 FvH4_4g04030 FvH4_4g04030
malus_domestica MD13G1216700.v1.1
prunus_persica Prupe.1G042400_v2.0.a1
pyrus_communis pycom13g19020
rosa_chinensis RchiOBHm_Chr4g0394301
rosa_laevigata RLG00000009705 RLG00000009706
rosa_multiflora Rmu_ssc0000423.1_g000004
rosa_roxburghii Rroxscaffold_5G00339330
rosa_rugosa Rorug03G0367500.1
rosa_samantha Rh4AG051500 Rh4BG048700 Rh4CG055400 Rh4DG048800
rosa_wichuraiana Rw4G004110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 1220
AciI CCGC 4 cut(s) 200, 604, 1940, 2027
AclWI GGATC 2 cut(s) 943, 1051
AcsI RAATTY 2 cut(s) 661, 1498
AcuI CTGAAG 6 cut(s) 66, 596, 911, 986, 1445, 1691
AfaI GTAC 1 cut(s) 1131
AfiI CCNNNNNNNGG 3 cut(s) 223, 444, 1604
Alw21I GWGCWC 1 cut(s) 1390
Alw26I GTCTC 2 cut(s) 1861, 2150
AlwI GGATC 2 cut(s) 943, 1051
AlwNI CAGNNNCTG 2 cut(s) 776, 1948
Ama87I CYCGRG 1 cut(s) 495
AoxI GGCC 8 cut(s) 249, 353, 605, 793, 1156, 1359, 1607, 1937
ApeKI GCWGC 5 cut(s) 770, 773, 776, 1348, 1823
ApoI RAATTY 2 cut(s) 661, 1498
AseI ATTAAT 1 cut(s) 963
Asp700I GAANNNNTTC 4 cut(s) 416, 680, 1116, 1618
AspS9I GGNCC 5 cut(s) 249, 354, 403, 1607, 1937
AsuC2I CCSGG 2 cut(s) 1611, 1723
AsuHPI GGTGA 5 cut(s) 161, 253, 515, 866, 2113
AvaI CYCGRG 1 cut(s) 495
AvaII GGWCC 1 cut(s) 403
BanI GGYRCC 1 cut(s) 1220
BanII GRGCYC 2 cut(s) 1390, 1634
BbsI GAAGAC 1 cut(s) 1656
Bbv12I GWGCWC 1 cut(s) 1390
BbvCI CCTCAGC 1 cut(s) 1975
BbvI GCAGC 5 cut(s) 760, 763, 782, 1335, 1835
BccI CCATC 6 cut(s) 221, 905, 1853, 1981, 2072, 2132
BceAI ACGGC 1 cut(s) 1208
BcgI CGANNNNNNTGC 2 cut(s) 1330, 1364
BciVI GTATCC 2 cut(s) 533, 1060
BclI TGATCA 4 cut(s) 568, 871, 1227, 2118
BcnI CCSGG 2 cut(s) 1611, 1723
BcoDI GTCTC 2 cut(s) 1861, 2150
BfaI CTAG 3 cut(s) 1154, 1416, 2094
BfmI CTRYAG 3 cut(s) 407, 1926, 1996
BfuI GTATCC 2 cut(s) 533, 1060
BisI GCNGC 8 cut(s) 201, 605, 771, 774, 777, 1349, 1824, 1940
BlsI GCNGC 8 cut(s) 202, 606, 772, 775, 778, 1350, 1825, 1941
BmcAI AGTACT 1 cut(s) 1131
Bme1390I CCNGG 2 cut(s) 1611, 1723
Bme18I GGWCC 1 cut(s) 403
BmeT110I CYCGRG 1 cut(s) 495
BmgT120I GGNCC 5 cut(s) 249, 354, 403, 1607, 1937
BmiI GGNNCC 3 cut(s) 313, 1222, 1720
BmrFI CCNGG 2 cut(s) 1611, 1723
BmsI GCATC 2 cut(s) 81, 1195
BpiI GAAGAC 1 cut(s) 1656
BpmI CTGGAG 1 cut(s) 1922
Bpu10I CCTNAGC 2 cut(s) 790, 1975
BpuMI CCSGG 2 cut(s) 1611, 1723
BsaBI GATNNNNATC 1 cut(s) 2123
BsaJI CCNNGG 3 cut(s) 1159, 1634, 1722
BsaXI ACNNNNNCTCC 2 cut(s) 750, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 444, 1604
Bse118I RCCGGY 1 cut(s) 1597
Bse1I ACTGG 3 cut(s) 1240, 1905, 1953
Bse3DI GCAATG 1 cut(s) 1068
Bse8I GATNNNNATC 1 cut(s) 2123
BseDI CCNNGG 3 cut(s) 1159, 1634, 1722
BseGI GGATG 5 cut(s) 16, 213, 1210, 1244, 2124
BseJI GATNNNNATC 1 cut(s) 2123
BseLI CCNNNNNNNGG 3 cut(s) 223, 444, 1604
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 8 cut(s) 78, 804, 852, 1086, 1239, 1389, 1856, 1989
BseNI ACTGG 3 cut(s) 1240, 1905, 1953
BseRI GAGGAG 3 cut(s) 311, 774, 1919
BseXI GCAGC 5 cut(s) 760, 763, 782, 1335, 1835
BseYI CCCAGC 1 cut(s) 2049
BshFI GGCC 8 cut(s) 251, 355, 607, 795, 1158, 1361, 1609, 1939
BshNI GGYRCC 1 cut(s) 1220
BsiHKAI GWGCWC 1 cut(s) 1390
BsiHKCI CYCGRG 1 cut(s) 495
BsiSI CCGG 3 cut(s) 1598, 1610, 1722
BslFI GGGAC 1 cut(s) 1195
BslI CCNNNNNNNGG 3 cut(s) 223, 444, 1604
BsmAI GTCTC 2 cut(s) 1861, 2150
BsmFI GGGAC 1 cut(s) 1195
BsmI GAATGC 3 cut(s) 514, 894, 1671
BsnI GGCC 8 cut(s) 251, 355, 607, 795, 1158, 1361, 1609, 1939
BsoBI CYCGRG 1 cut(s) 495
Bsp1286I GDGCHC 2 cut(s) 1390, 1634
Bsp143I GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
BspACI CCGC 4 cut(s) 200, 604, 1940, 2027
BspANI GGCC 8 cut(s) 251, 355, 607, 795, 1158, 1361, 1609, 1939
BspCNI CTCAG 8 cut(s) 79, 803, 851, 1087, 1240, 1388, 1857, 1988
BspLI GGNNCC 3 cut(s) 313, 1222, 1720
BspMAI CTGCAG 2 cut(s) 411, 1930
BspPI GGATC 2 cut(s) 943, 1051
BspT107I GGYRCC 1 cut(s) 1220
BsrDI GCAATG 1 cut(s) 1068
BsrFI RCCGGY 1 cut(s) 1597
BsrI ACTGG 3 cut(s) 1240, 1905, 1953
BssAI RCCGGY 1 cut(s) 1597
BssECI CCNNGG 3 cut(s) 1159, 1634, 1722
BssMI GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
BssT1I CCWWGG 2 cut(s) 1159, 1634
Bst4CI ACNGT 5 cut(s) 68, 907, 943, 1072, 2144
Bst6I CTCTTC 7 cut(s) 31, 85, 615, 853, 1337, 1738, 2072
BstAPI GCANNNNNTGC 1 cut(s) 776
BstC8I GCNNGC 4 cut(s) 450, 531, 787, 1803
BstF5I GGATG 5 cut(s) 16, 213, 1210, 1244, 2124
BstKTI GATC 8 cut(s) 571, 874, 938, 1021, 1029, 1046, 1230, 2121
BstMAI GTCTC 2 cut(s) 1861, 2150
BstMBI GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
BstMWI GCNNNNNNNGC 4 cut(s) 776, 782, 1385, 1829
BstSCI CCNGG 2 cut(s) 1609, 1721
BstSFI CTRYAG 3 cut(s) 407, 1926, 1996
BstV1I GCAGC 5 cut(s) 760, 763, 782, 1335, 1835
BstV2I GAAGAC 1 cut(s) 1656
BsuI GTATCC 2 cut(s) 533, 1060
BsuRI GGCC 8 cut(s) 251, 355, 607, 795, 1158, 1361, 1609, 1939
BtsCI GGATG 5 cut(s) 16, 213, 1210, 1244, 2124
BtsI GCAGTG 1 cut(s) 1215
BtsIMutI CAGTG 3 cut(s) 1215, 1551, 1946
Cac8I GCNNGC 4 cut(s) 450, 531, 787, 1803
CaiI CAGNNNCTG 2 cut(s) 776, 1948
Cfr10I RCCGGY 1 cut(s) 1597
Cfr13I GGNCC 5 cut(s) 249, 354, 403, 1607, 1937
Csp6I GTAC 1 cut(s) 1130
CviAII CATG 7 cut(s) 71, 351, 545, 869, 1194, 1918, 2116
CviQI GTAC 1 cut(s) 1130
DpnI GATC 8 cut(s) 570, 873, 937, 1020, 1028, 1045, 1229, 2120
DpnII GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
Eam1104I CTCTTC 7 cut(s) 31, 85, 615, 853, 1337, 1738, 2072
EarI CTCTTC 7 cut(s) 31, 85, 615, 853, 1337, 1738, 2072
Ecl136II GAGCTC 1 cut(s) 1388
Eco130I CCWWGG 2 cut(s) 1159, 1634
Eco147I AGGCCT 1 cut(s) 1158
Eco24I GRGCYC 2 cut(s) 1390, 1634
Eco47I GGWCC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 1388
Eco57I CTGAAG 6 cut(s) 66, 596, 911, 986, 1445, 1691
Eco88I CYCGRG 1 cut(s) 495
EcoICRI GAGCTC 1 cut(s) 1388
EcoO109I RGGNCCY 2 cut(s) 249, 403
EcoRI GAATTC 1 cut(s) 661
EcoT14I CCWWGG 2 cut(s) 1159, 1634
EcoT38I GRGCYC 2 cut(s) 1390, 1634
ErhI CCWWGG 2 cut(s) 1159, 1634
FaeI CATG 7 cut(s) 74, 354, 548, 872, 1197, 1921, 2119
FaqI GGGAC 1 cut(s) 1195
FatI CATG 7 cut(s) 70, 350, 544, 868, 1193, 1917, 2115
FbaI TGATCA 4 cut(s) 568, 871, 1227, 2118
Fnu4HI GCNGC 8 cut(s) 201, 605, 771, 774, 777, 1349, 1824, 1940
FokI GGATG 5 cut(s) 3, 200, 1217, 1251, 2111
FriOI GRGCYC 2 cut(s) 1390, 1634
Fsp4HI GCNGC 8 cut(s) 201, 605, 771, 774, 777, 1349, 1824, 1940
FspBI CTAG 3 cut(s) 1154, 1416, 2094
GluI GCNGC 8 cut(s) 201, 605, 771, 774, 777, 1349, 1824, 1940
GsaI CCCAGC 1 cut(s) 2053
GsuI CTGGAG 1 cut(s) 1922
HaeIII GGCC 8 cut(s) 251, 355, 607, 795, 1158, 1361, 1609, 1939
HapII CCGG 3 cut(s) 1598, 1610, 1722
Hin1II CATG 7 cut(s) 74, 354, 548, 872, 1197, 1921, 2119
HpaII CCGG 3 cut(s) 1598, 1610, 1722
HphI GGTGA 5 cut(s) 161, 253, 515, 866, 2113
Hpy166II GTNNAC 4 cut(s) 152, 364, 460, 1421
Hpy188I TCNGA 9 cut(s) 573, 615, 660, 841, 1048, 1446, 1504, 1854, 1986
Hpy188III TCNNGA 5 cut(s) 812, 888, 1004, 1680, 1913
Hpy8I GTNNAC 4 cut(s) 152, 364, 460, 1421
HpyAV CCTTC 8 cut(s) 193, 367, 610, 665, 1106, 1729, 1928, 2015
HpyCH4III ACNGT 5 cut(s) 68, 907, 943, 1072, 2144
HpyCH4IV ACGT 2 cut(s) 720, 732
HpyF10VI GCNNNNNNNGC 4 cut(s) 776, 782, 1385, 1829
HpySE526I ACGT 2 cut(s) 720, 732
Hsp92II CATG 7 cut(s) 74, 354, 548, 872, 1197, 1921, 2119
Ksp22I TGATCA 4 cut(s) 568, 871, 1227, 2118
Kzo9I GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
LmnI GCTCC 4 cut(s) 865, 1385, 1829, 1992
Lsp1109I GCAGC 5 cut(s) 760, 763, 782, 1335, 1835
LweI GCATC 2 cut(s) 81, 1195
MaeI CTAG 3 cut(s) 1154, 1416, 2094
MaeII ACGT 2 cut(s) 720, 732
MaeIII GTNAC 7 cut(s) 728, 901, 1090, 1366, 1729, 1921, 1944
MalI GATC 8 cut(s) 570, 873, 937, 1020, 1028, 1045, 1229, 2120
MboI GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
MfeI CAATTG 1 cut(s) 1833
MhlI GDGCHC 2 cut(s) 1390, 1634
MlyI GAGTC 3 cut(s) 1487, 1562, 1863
MmeI TCCRAC 2 cut(s) 366, 1424
MroXI GAANNNNTTC 4 cut(s) 416, 680, 1116, 1618
MseI TTAA 7 cut(s) 165, 963, 981, 1406, 1469, 1808, 2109
MslI CAYNNNNRTG 2 cut(s) 879, 1854
MspA1I CMGCKG 2 cut(s) 86, 773
MspI CCGG 3 cut(s) 1598, 1610, 1722
MspR9I CCNGG 2 cut(s) 1611, 1723
MunI CAATTG 1 cut(s) 1833
Mva1269I GAATGC 3 cut(s) 514, 894, 1671
MwoI GCNNNNNNNGC 4 cut(s) 776, 782, 1385, 1829
NciI CCSGG 2 cut(s) 1611, 1723
NdeII GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
NlaIII CATG 7 cut(s) 74, 354, 548, 872, 1197, 1921, 2119
NlaIV GGNNCC 3 cut(s) 313, 1222, 1720
NmuCI GTSAC 2 cut(s) 728, 1944
PceI AGGCCT 1 cut(s) 1158
PctI GAATGC 3 cut(s) 514, 894, 1671
PdmI GAANNNNTTC 4 cut(s) 416, 680, 1116, 1618
PfeI GAWTC 9 cut(s) 75, 621, 884, 1150, 1187, 1447, 1813, 2007, 2165
PkrI GCNGC 8 cut(s) 202, 606, 772, 775, 778, 1350, 1825, 1941
PleI GAGTC 3 cut(s) 1487, 1562, 1863
PpsI GAGTC 3 cut(s) 1487, 1562, 1863
PpuMI RGGWCCY 1 cut(s) 403
PshBI ATTAAT 1 cut(s) 963
PsiI TTATAA 1 cut(s) 737
Psp124BI GAGCTC 1 cut(s) 1390
Psp5II RGGWCCY 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 2049
PspN4I GGNNCC 3 cut(s) 313, 1222, 1720
PspPI GGNCC 5 cut(s) 249, 354, 403, 1607, 1937
PspPPI RGGWCCY 1 cut(s) 403
PstI CTGCAG 2 cut(s) 411, 1930
PstNI CAGNNNCTG 2 cut(s) 776, 1948
PvuII CAGCTG 2 cut(s) 86, 773
RsaI GTAC 1 cut(s) 1131
RsaNI GTAC 1 cut(s) 1130
RseI CAYNNNNRTG 2 cut(s) 879, 1854
SacI GAGCTC 1 cut(s) 1390
SaqAI TTAA 7 cut(s) 165, 963, 981, 1406, 1469, 1808, 2109
SatI GCNGC 8 cut(s) 201, 605, 771, 774, 777, 1349, 1824, 1940
Sau3AI GATC 8 cut(s) 568, 871, 935, 1018, 1026, 1043, 1227, 2118
Sau96I GGNCC 5 cut(s) 249, 354, 403, 1607, 1937
ScaI AGTACT 1 cut(s) 1131
SchI GAGTC 3 cut(s) 1487, 1562, 1863
ScrFI CCNGG 2 cut(s) 1611, 1723
SduI GDGCHC 2 cut(s) 1390, 1634
SfaNI GCATC 2 cut(s) 81, 1195
SfcI CTRYAG 3 cut(s) 407, 1926, 1996
SinI GGWCC 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 879, 1854
SseBI AGGCCT 1 cut(s) 1158
SsiI CCGC 4 cut(s) 200, 604, 1940, 2027
SspI AATATT 1 cut(s) 712
SspMI CTAG 3 cut(s) 1154, 1416, 2094
SstI GAGCTC 1 cut(s) 1390
StuI AGGCCT 1 cut(s) 1158
StyD4I CCNGG 2 cut(s) 1609, 1721
StyI CCWWGG 2 cut(s) 1159, 1634
TaaI ACNGT 5 cut(s) 68, 907, 943, 1072, 2144
TaiI ACGT 2 cut(s) 723, 735
TaqI TCGA 1 cut(s) 813
TatI WGTACW 1 cut(s) 1129
TauI GCSGC 3 cut(s) 203, 607, 1942
TfiI GAWTC 9 cut(s) 75, 621, 884, 1150, 1187, 1447, 1813, 2007, 2165
Tru1I TTAA 7 cut(s) 165, 963, 981, 1406, 1469, 1808, 2109
Tru9I TTAA 7 cut(s) 165, 963, 981, 1406, 1469, 1808, 2109
TscAI CASTG 3 cut(s) 1215, 1551, 1953
TseFI GTSAC 2 cut(s) 728, 1944
TseI GCWGC 5 cut(s) 770, 773, 776, 1348, 1823
Tsp45I GTSAC 2 cut(s) 728, 1944
TspDTI ATGAA 4 cut(s) 153, 897, 1442, 1947
TspRI CASTG 3 cut(s) 1215, 1551, 1953
VpaK11BI GGWCC 1 cut(s) 403
VspI ATTAAT 1 cut(s) 963
XapI RAATTY 2 cut(s) 661, 1498
XmnI GAANNNNTTC 4 cut(s) 416, 680, 1116, 1618
XspI CTAG 3 cut(s) 1154, 1416, 2094
ZrmI AGTACT 1 cut(s) 1131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.