Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006981

Physical Location & Seq

Genomic Coordinates Reverse (-)

26054386 .. 26062754

Gene Structure 8369 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1247200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2868 bp

ATGGGGAGTACGGGCAAGGCTATCAGCCTGGAGGAGATCAAGAACGAGACTGTTGATCTGGAGCGGATTCCGGTCGAGGAAGTGTTTGAACAGTTGAAATGTACCAAAGAAGGCTTGAGCTCGGAGGAAGGAGCCCAAAGGCTAGAGATTTTTGGTCCGAACAAGCTAGAAGAGAAAAAGGAAAGCAAATTTCTCAAGTTCCTGGGGTTTATGTGGAATCCTCTATCATGGGTCATGGAAGCTGCAGCCATCATGGCAATTGCTCTGGCCAACGGCGATGGGAAGCCGCCAGATTGGCAAGATTTTGTCGGTATTCTCTGCCTGTTGGTCATCAACTCTACCATCAGTTTTATTGAAGAAAACAATGCCGGAAATGCAGCTGCTGCTCTTATGGCTGGTCTTGCTCCTAAGACTAAGGTGCTTAGAGATGGAAAATGGAGCGAGGAGGATGCTGCGATCTTGGTTCCAGGGGACATCATCAGTATCAAATTGGGAGATATCGTCCCAGCTGATGCTCGTCTTCTCGAGGGTGATCCTTTAAAGATTGATCAATCTGCCCTCACAGGAGAATCACTTCCAGTGACCAAGCATCCAGGTGATGAGGTTTTCTCCGGTTCAACTTGCAAGCAGGGTGAAATTGAAGCTGTTGTTATTGCCACCGGTGTCCATACCTTCTTCGGGAAGGCTGCACATCTTGTGGACAGCACTAACCAAGTCGGACACTTCCAGAAGGTGCTCACTGCCATCGGGAACTTCTGTATCTGTTCCATTGCCATTGGAATGGTGATTGAGATCATAGTCATGTACCCAATTCAGCACCGCAAGTACAGGAATGGAATTGACAATCTCTTGGTGCTCTTGATTGGAGGCATTCCCATTGCCATGCCCACTGTCTTGTCCGTGACAATGGCTATTGGTTCTCACAGGCTATCTCAGCAGGGTGCCATCACCAAGAGGATGACTGCCATTGAGGAAATGGCGGGTATGGATGTACTTTGCAGTGATAAGACAGGAACACTAACTCTTAACAAGCTTAGTGTCGATAAAAACTTGATTGAGGTGTTTGCAAAGGGTGTGGAGAAAGATCATGTCATTCTTCTAGCTGCAAGGTCGTCCAGAACTGAAAACCAGGATGCCATTGATGCTGCTATGGTTGGAATGCTTGCCGACCCAAAGGAGGCCCGAGCTGGTATTAGAGAGGTGCACTTCTTACCATTTAATCCCGTCGACAAGAGAACTGCTTTGACTTACATTGATTCCGATGGAAACTGGCATAGAGCAAGCAAAGGTGCCCCTGAGCAGATTTTGGACCTCTGCCTCTGCAAAGAAGATTTCAAGAAGAAGGCTTTTGCCATCATTGACAAATATGCTGAACGTGGGCTTCGGTCATTGGCTGTTGCCAGACAGGAAGTGCCTGCGAAAACAAAAGAAAGTTCTGGTGGTCCATGGCAGTTTGTTGGATTGTTGCCTCTTTTCGACCCTCCAAGACATGATAGTGCAGAAACCATCCGCCAGGCTCTTAACCTTGGTGTAAATGTTAAGATGATTACTGGCGATCAACTTGCCATTGCCAAGGAAACTGGTCGGAGACTTGGAATGGGAACAAACATGTACCCATCTGCTTCCTTACTTGGTCAACACAAGGATGCAAGCATCGCTGCCCTTCCTATTGAAGAGTTAATTGAGAAGGCTGACGGGTTCGCTGGAGTGTTTCCAGAGCACAAATACGAAATTGTGAAGAAGTTGCAGGAAAGAAAGCACATTTGTGGAATGACTGGAGATGGTGTCAATGATGCCCCTGCACTGAAGAAGGCAGATATCGGAATTGCTGTTGCCGATGCTACCGATGCTGCACGAAGTGCTTCCGACATTGTTCTTACAGAACCCGGATTGAGTGTCATCATTAGTGCAGTGTTGACCAGTAGAGCTATTTTCCAGAGAATGAAGAACTACACTATCTATGCAGTTTCTATCACCATTCGTATTGTGTTCGGTTTCATGTTCATTGCTCTCATATGGAAGTTCGACTTCTCACCCTTCATGGTTTTGATTATTGCCATATTGAACGATGGCACAATTATGACAATCTCAAAGGATAGAGTAAAGCCATCTCCCCTGCCTGATAGCTGGAAATTGAAAGAGATTTTTGCTACTGGGATTGTGCTTGGAGGTTACTTGGCACTGATGACTGTTATCTTCTTCTGGTTAATCAAAGAAACCGACTTCTTCTCTGACTCATTTGGTGTAAGATCCATAAGGGAGAAACCTGAGGAACTAATGGCTGCCTTGTACCTGCAAGTGAGTATTGTGAGCCAGGCCCTCATTTTTGTGACTCGATCCCGTGGCTTCTCCTTTATGGAACGACCTGGAATGCTTCTAATGAGTGCCTTCATGATCGCGCAACTGATTGCAACTTTGCTAGCAGTATATGCCAAATGGGGTTTCGCAAAAATACACGGAGTTGGTTGGGGATGGGCTGGTGTCATCTGGATTTACAGCATTGTCTTCTATTTCCCACTAGACTTTATGAAATTCGCCATTCGGTATATCTTGAGTGGAAAGGCGTGGCTCAACTTGCTAGAGAACAAGACTGCCTTTACCACCAAGAAAGATTATGGAAAAGAAGAAAGAGAAGCTCAATGGGCTCATGCTCAAAGGACACTTCACGGTCTCCAAGCACCGGAATCTTCTAGTATCTTCCATGACAAGAACAGCTACAGAGAGCTGTCTGAGATTGCTGAGCAGGCCAAGAGACGAGCCGAAGTTGCAAGACTTCGGGAGCTTCACACTCTCAAGGGACATGTGGAATCAGTGGTGAAACTGAAGGGCCTGGACATTGACACAATCCAGCAGCATTATACAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

956

Amino Acids

105.21

Weight (kDa)

6.51

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	22 - 85	5.5e-16	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	135 - 233	3.7e-31	P-type ATPase actuator domain
Hydrolase	PF00702	329 - 606	4.4e-15	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18960 AT2G18960 AT2G18960 AT4G30190 AT4G30190 AT5G57350 AT5G57350 AT5G57350 AT5G57350
fragaria_vesca	FvH4_4g07810 FvH4_4g07810
malus_domestica	MD13G1247200.v1.1 MD16G1254200.v1.1
prunus_persica	Prupe.1G082000_v2.0.a1
pyrus_communis	pycom12g18290
rosa_chinensis	RchiOBHm_Chr4g0400911
rosa_laevigata	RLG00000009165
rosa_multiflora	Rmu_sc0002026.1_g000039
rosa_roxburghii	Rroxscaffold_5G00345810
rosa_rugosa	Rorug04G0027100
rosa_samantha	Rh4AG104700 Rh4BG099900 Rh4CG113100 Rh4DG096900
rosa_wichuraiana	Rw4G008480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2301
AccB1I	GGYRCC	2 cut(s)	941, 1289
AccBSI	CCGCTC	1 cut(s)	64
AccI	GTMKAC	1 cut(s)	1227
AccII	CGCG	1 cut(s)	2399
AciI	CCGC	5 cut(s)	64, 287, 820, 980, 1510
AclWI	GGATC	3 cut(s)	527, 2244, 2331
AcoI	YGGCCR	1 cut(s)	267
AcsI	RAATTY	2 cut(s)	188, 2531
AcuI	CTGAAG	2 cut(s)	1826, 2843
AdeI	CACNNNGTG	1 cut(s)	1859
AfaI	GTAC	7 cut(s)	10, 103, 806, 827, 993, 1613, 2291
AfiI	CCNNNNNNNGG	3 cut(s)	678, 1177, 2680
AflIII	ACRYGT	2 cut(s)	1608, 2800
AgeI	ACCGGT	1 cut(s)	659
AgsI	TTSAA	9 cut(s)	89, 97, 356, 618, 641, 1336, 1673, 2065, 2137
AjnI	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
AjuI	GAANNNNNNNTTGG	2 cut(s)	2426, 2458
AleI	CACNNNNGTG	1 cut(s)	1764
Alw21I	GWGCWC	5 cut(s)	122, 738, 858, 1206, 1722
Alw26I	GTCTC	4 cut(s)	41, 1582, 2675, 2746
Alw44I	GTGCAC	1 cut(s)	1202
AlwI	GGATC	3 cut(s)	527, 2244, 2331
AlwNI	CAGNNNCTG	1 cut(s)	383
Ama87I	CYCGRG	2 cut(s)	524, 1182
AoxI	GGCC	5 cut(s)	267, 1179, 2316, 2745, 2827
ApaLI	GTGCAC	1 cut(s)	1202
ApoI	RAATTY	2 cut(s)	188, 2531
AsiGI	ACCGGT	1 cut(s)	659
Asp700I	GAANNNNTTC	2 cut(s)	573, 1861
AspLEI	GCGC	1 cut(s)	2401
AspS9I	GGNCC	6 cut(s)	155, 1180, 1309, 1442, 2317, 2827
AsuC2I	CCSGG	1 cut(s)	1887
AsuHPI	GGTGA	8 cut(s)	542, 608, 644, 796, 940, 1966, 2025, 2827
AsuNHI	GCTAGC	1 cut(s)	2419
AvaI	CYCGRG	2 cut(s)	524, 1182
AvaII	GGWCC	3 cut(s)	155, 1309, 1442
AxyI	CCTNAGG	1 cut(s)	2268
BaeGI	GKGCMC	2 cut(s)	1206, 1294
BaeI	ACNNNNGTAYC	2 cut(s)	742, 775
BalI	TGGCCA	1 cut(s)	269
BanI	GGYRCC	2 cut(s)	941, 1289
BanII	GRGCYC	3 cut(s)	122, 136, 2647
BbsI	GAAGAC	2 cut(s)	512, 2497
Bbv12I	GWGCWC	5 cut(s)	122, 738, 858, 1206, 1722
BceAI	ACGGC	1 cut(s)	289
BcgI	CGANNNNNNTGC	4 cut(s)	431, 465, 1544, 1578
BciT130I	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BclI	TGATCA	1 cut(s)	547
BcnI	CCSGG	1 cut(s)	1887
BcoDI	GTCTC	4 cut(s)	41, 1582, 2675, 2746
BfaI	CTAG	7 cut(s)	143, 167, 1100, 2420, 2519, 2579, 2691
BfmI	CTRYAG	2 cut(s)	243, 2716
BfuAI	ACCTGC	1 cut(s)	2301
BglI	GCCNNNNNGGC	2 cut(s)	254, 295
BlpI	GCTNAGC	1 cut(s)	2739
Bme18I	GGWCC	3 cut(s)	155, 1309, 1442
BmeT110I	CYCGRG	2 cut(s)	524, 1182
BmgT120I	GGNCC	6 cut(s)	155, 1180, 1309, 1442, 2317, 2827
BmiI	GGNNCC	4 cut(s)	133, 465, 943, 1291
BmrI	ACTGGG	1 cut(s)	2163
BmtI	GCTAGC	1 cut(s)	2423
BmuI	ACTGGG	1 cut(s)	2163
BpiI	GAAGAC	2 cut(s)	512, 2497
BplI	GAGNNNNNCTC	2 cut(s)	593, 625
BpmI	CTGGAG	4 cut(s)	50, 80, 1725, 1797
Bpu10I	CCTNAGC	1 cut(s)	1296
Bpu1102I	GCTNAGC	1 cut(s)	2739
BpuEI	CTTGAG	4 cut(s)	136, 179, 2572, 2777
BpuMI	CCSGG	1 cut(s)	1887
BsaBI	GATNNNNATC	1 cut(s)	791
BsaI	GGTCTC	1 cut(s)	2675
BsaJI	CCNNGG	6 cut(s)	202, 467, 1445, 1525, 1572, 2341
BsaWI	WCCGGW	4 cut(s)	70, 611, 659, 2680
BsaXI	ACNNNNNCTCC	4 cut(s)	486, 516, 1770, 1800
Bsc4I	CCNNNNNNNGG	3 cut(s)	678, 1177, 2680
Bse118I	RCCGGY	1 cut(s)	659
Bse1I	ACTGG	7 cut(s)	578, 1274, 1555, 1585, 1780, 1920, 2158
Bse21I	CCTNAGG	1 cut(s)	2268
Bse3DI	GCAATG	4 cut(s)	768, 876, 1566, 2004
Bse8I	GATNNNNATC	1 cut(s)	791
BseBI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BseDI	CCNNGG	6 cut(s)	202, 467, 1445, 1525, 1572, 2341
BseGI	GGATG	8 cut(s)	454, 589, 963, 994, 1138, 1506, 1651, 2477
BseJI	GATNNNNATC	1 cut(s)	791
BseLI	CCNNNNNNNGG	3 cut(s)	678, 1177, 2680
BseMI	GCAATG	4 cut(s)	768, 876, 1566, 2004
BseMII	CTCAG	5 cut(s)	947, 1287, 2259, 2721, 2730
BseNI	ACTGG	7 cut(s)	578, 1274, 1555, 1585, 1780, 1920, 2158
BseRI	GAGGAG	2 cut(s)	47, 458
BseSI	GKGCMC	2 cut(s)	1206, 1294
BseYI	CCCAGC	1 cut(s)	505
BsgI	GTGCAG	5 cut(s)	672, 1518, 1785, 1836, 1929
Bsh1236I	CGCG	1 cut(s)	2399
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	5 cut(s)	269, 1181, 2318, 2747, 2829
BshNI	GGYRCC	2 cut(s)	941, 1289
BshTI	ACCGGT	1 cut(s)	659
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	5 cut(s)	122, 738, 858, 1206, 1722
BsiHKCI	CYCGRG	2 cut(s)	524, 1182
BsiSI	CCGG	6 cut(s)	71, 369, 612, 660, 1887, 2681
BslFI	GGGAC	3 cut(s)	485, 488, 2811
BslI	CCNNNNNNNGG	3 cut(s)	678, 1177, 2680
BsmAI	GTCTC	4 cut(s)	41, 1582, 2675, 2746
BsmBI	CGTCTC	1 cut(s)	2746
BsmFI	GGGAC	3 cut(s)	485, 488, 2811
BsmI	GAATGC	3 cut(s)	870, 1164, 2376
BsnI	GGCC	5 cut(s)	269, 1181, 2318, 2747, 2829
Bso31I	GGTCTC	1 cut(s)	2675
BsoBI	CYCGRG	2 cut(s)	524, 1182
Bsp1286I	GDGCHC	8 cut(s)	122, 136, 738, 858, 1206, 1294, 1722, 2647
Bsp1720I	GCTNAGC	1 cut(s)	2739
Bsp19I	CCATGG	1 cut(s)	1445
BspACI	CCGC	5 cut(s)	64, 287, 820, 980, 1510
BspANI	GGCC	5 cut(s)	269, 1181, 2318, 2747, 2829
BspCNI	CTCAG	5 cut(s)	946, 1288, 2260, 2722, 2731
BspFNI	CGCG	1 cut(s)	2399
BspHI	TCATGA	1 cut(s)	2391
BspLI	GGNNCC	4 cut(s)	133, 465, 943, 1291
BspMAI	CTGCAG	1 cut(s)	247
BspMI	ACCTGC	1 cut(s)	2301
BspOI	GCTAGC	1 cut(s)	2423
BspPI	GGATC	3 cut(s)	527, 2244, 2331
BspT107I	GGYRCC	2 cut(s)	941, 1289
BspTNI	GGTCTC	1 cut(s)	2675
BsrBI	CCGCTC	1 cut(s)	64
BsrDI	GCAATG	4 cut(s)	768, 876, 1566, 2004
BsrFI	RCCGGY	1 cut(s)	659
BsrI	ACTGG	7 cut(s)	578, 1274, 1555, 1585, 1780, 1920, 2158
BssAI	RCCGGY	1 cut(s)	659
BssECI	CCNNGG	6 cut(s)	202, 467, 1445, 1525, 1572, 2341
BssT1I	CCWWGG	3 cut(s)	1445, 1525, 1572
Bst2UI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
Bst4CI	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
Bst6I	CTCTTC	2 cut(s)	165, 1668
BstAPI	GCANNNNNTGC	3 cut(s)	383, 1859, 2429
BstC8I	GCNNGC	7 cut(s)	626, 1164, 1282, 1416, 1651, 2421, 2745
BstDEI	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
BstDSI	CCRYGG	2 cut(s)	1445, 2341
BstF5I	GGATG	8 cut(s)	454, 589, 963, 994, 1138, 1506, 1651, 2477
BstFNI	CGCG	1 cut(s)	2399
BstHHI	GCGC	1 cut(s)	2401
BstMAI	GTCTC	4 cut(s)	41, 1582, 2675, 2746
BstMCI	CGRYCG	1 cut(s)	75
BstNI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BstNSI	RCATGY	2 cut(s)	1612, 2804
BstSFI	CTRYAG	2 cut(s)	243, 2716
BstSLI	GKGCMC	2 cut(s)	1206, 1294
BstUI	CGCG	1 cut(s)	2399
BstV2I	GAAGAC	2 cut(s)	512, 2497
BstX2I	RGATCY	1 cut(s)	2249
BstXI	CCANNNNNNTGG	1 cut(s)	781
BstYI	RGATCY	1 cut(s)	2249
Bsu36I	CCTNAGG	1 cut(s)	2268
BsuRI	GGCC	5 cut(s)	269, 1181, 2318, 2747, 2829
BtgI	CCRYGG	2 cut(s)	1445, 2341
BtgZI	GCGATG	2 cut(s)	291, 1639
BtsCI	GGATG	8 cut(s)	454, 589, 963, 994, 1138, 1506, 1651, 2477
BtsI	GCAGTG	3 cut(s)	738, 1006, 1917
BtsIMutI	CAGTG	9 cut(s)	585, 738, 888, 1006, 1802, 1917, 2180, 2817, 2868
BveI	ACCTGC	1 cut(s)	2301
Cac8I	GCNNGC	7 cut(s)	626, 1164, 1282, 1416, 1651, 2421, 2745
CaiI	CAGNNNCTG	1 cut(s)	383
CciI	TCATGA	1 cut(s)	2391
CfoI	GCGC	1 cut(s)	2401
Cfr10I	RCCGGY	1 cut(s)	659
Cfr13I	GGNCC	6 cut(s)	155, 1180, 1309, 1442, 2317, 2827
Csp6I	GTAC	7 cut(s)	9, 102, 805, 826, 992, 1612, 2290
CspAI	ACCGGT	1 cut(s)	659
CspCI	CAANNNNNGTGG	2 cut(s)	1056, 1091
CviQI	GTAC	7 cut(s)	9, 102, 805, 826, 992, 1612, 2290
DdeI	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
DraI	TTTAAA	1 cut(s)	540
DraIII	CACNNNGTG	1 cut(s)	1859
EaeI	YGGCCR	1 cut(s)	267
Eam1104I	CTCTTC	2 cut(s)	165, 1668
EarI	CTCTTC	2 cut(s)	165, 1668
EciI	GGCGGA	1 cut(s)	1499
Ecl136II	GAGCTC	1 cut(s)	120
Eco130I	CCWWGG	3 cut(s)	1445, 1525, 1572
Eco24I	GRGCYC	3 cut(s)	122, 136, 2647
Eco31I	GGTCTC	1 cut(s)	2675
Eco32I	GATATC	2 cut(s)	499, 1819
Eco47I	GGWCC	3 cut(s)	155, 1309, 1442
Eco53kI	GAGCTC	1 cut(s)	120
Eco57I	CTGAAG	2 cut(s)	1826, 2843
Eco81I	CCTNAGG	1 cut(s)	2268
Eco88I	CYCGRG	2 cut(s)	524, 1182
EcoICRI	GAGCTC	1 cut(s)	120
EcoO109I	RGGNCCY	2 cut(s)	2317, 2827
EcoRII	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
EcoRV	GATATC	2 cut(s)	499, 1819
EcoT14I	CCWWGG	3 cut(s)	1445, 1525, 1572
EcoT38I	GRGCYC	3 cut(s)	122, 136, 2647
ErhI	CCWWGG	3 cut(s)	1445, 1525, 1572
Esp3I	CGTCTC	1 cut(s)	2746
FalI	AAGNNNNNCTT	4 cut(s)	2012, 2044, 2579, 2611
FaqI	GGGAC	3 cut(s)	485, 488, 2811
FauI	CCCGC	1 cut(s)	973
FauNDI	CATATG	1 cut(s)	2015
FbaI	TGATCA	1 cut(s)	547
FblI	GTMKAC	1 cut(s)	1227
FokI	GGATG	8 cut(s)	461, 576, 970, 1001, 1145, 1493, 1658, 2484
FriOI	GRGCYC	3 cut(s)	122, 136, 2647
FspBI	CTAG	7 cut(s)	143, 167, 1100, 2420, 2519, 2579, 2691
GlaI	GCGC	1 cut(s)	2400
GsaI	CCCAGC	1 cut(s)	509
GsuI	CTGGAG	4 cut(s)	50, 80, 1725, 1797
HaeIII	GGCC	5 cut(s)	269, 1181, 2318, 2747, 2829
HapII	CCGG	6 cut(s)	71, 369, 612, 660, 1887, 2681
HhaI	GCGC	1 cut(s)	2401
Hin6I	GCGC	1 cut(s)	2399
HinP1I	GCGC	1 cut(s)	2399
HincII	GTYRAC	3 cut(s)	1228, 1637, 1917
HindII	GTYRAC	3 cut(s)	1228, 1637, 1917
HindIII	AAGCTT	1 cut(s)	1031
HinfI	GANTC	8 cut(s)	67, 217, 569, 1256, 2234, 2332, 2684, 2807
HpaII	CCGG	6 cut(s)	71, 369, 612, 660, 1887, 2681
HphI	GGTGA	8 cut(s)	542, 608, 644, 796, 940, 1966, 2025, 2827
Hpy166II	GTNNAC	5 cut(s)	700, 1204, 1228, 1637, 1917
Hpy188I	TCNGA	9 cut(s)	124, 159, 719, 1261, 1587, 1823, 1867, 2233, 2731
Hpy8I	GTNNAC	5 cut(s)	700, 1204, 1228, 1637, 1917
Hpy99I	CGWCG	1 cut(s)	1229
HpyCH4III	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
HpyCH4IV	ACGT	1 cut(s)	1375
HpyF3I	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
HpySE526I	ACGT	1 cut(s)	1375
HspAI	GCGC	1 cut(s)	2399
Ksp22I	TGATCA	1 cut(s)	547
LmnI	GCTCC	5 cut(s)	61, 131, 409, 438, 2779
MaeI	CTAG	7 cut(s)	143, 167, 1100, 2420, 2519, 2579, 2691
MaeII	ACGT	1 cut(s)	1375
MaeIII	GTNAC	4 cut(s)	580, 901, 2171, 2329
MbiI	CCGCTC	1 cut(s)	64
MfeI	CAATTG	1 cut(s)	258
MflI	RGATCY	1 cut(s)	2249
MhlI	GDGCHC	8 cut(s)	122, 136, 738, 858, 1206, 1294, 1722, 2647
MlsI	TGGCCA	1 cut(s)	269
MluNI	TGGCCA	1 cut(s)	269
MlyI	GAGTC	2 cut(s)	2228, 2326
MmeI	TCCRAC	5 cut(s)	697, 1135, 1438, 1565, 1890
Mox20I	TGGCCA	1 cut(s)	269
MroXI	GAANNNNTTC	2 cut(s)	573, 1861
MscI	TGGCCA	1 cut(s)	269
MseI	TTAA	7 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207
MslI	CAYNNNNRTG	9 cut(s)	594, 779, 800, 881, 1494, 1644, 1764, 2078, 2327
Msp20I	TGGCCA	1 cut(s)	269
MspA1I	CMGCKG	2 cut(s)	380, 509
MspI	CCGG	6 cut(s)	71, 369, 612, 660, 1887, 2681
MunI	CAATTG	1 cut(s)	258
Mva1269I	GAATGC	3 cut(s)	870, 1164, 2376
MvaI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
MvnI	CGCG	1 cut(s)	2399
NciI	CCSGG	1 cut(s)	1887
NcoI	CCATGG	1 cut(s)	1445
NdeI	CATATG	1 cut(s)	2015
NheI	GCTAGC	1 cut(s)	2419
NlaIV	GGNNCC	4 cut(s)	133, 465, 943, 1291
NmuCI	GTSAC	3 cut(s)	580, 901, 2329
NspI	RCATGY	2 cut(s)	1612, 2804
OliI	CACNNNNGTG	1 cut(s)	1764
PaeR7I	CTCGAG	1 cut(s)	524
PagI	TCATGA	1 cut(s)	2391
PciI	ACATGT	2 cut(s)	1608, 2800
PctI	GAATGC	3 cut(s)	870, 1164, 2376
PdmI	GAANNNNTTC	2 cut(s)	573, 1861
PfeI	GAWTC	6 cut(s)	67, 217, 569, 1256, 2684, 2807
PinAI	ACCGGT	1 cut(s)	659
PleI	GAGTC	2 cut(s)	2228, 2326
PpsI	GAGTC	2 cut(s)	2228, 2326
PscI	ACATGT	2 cut(s)	1608, 2800
Psp124BI	GAGCTC	1 cut(s)	122
Psp6I	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
PspFI	CCCAGC	1 cut(s)	505
PspGI	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
PspN4I	GGNNCC	4 cut(s)	133, 465, 943, 1291
PspPI	GGNCC	6 cut(s)	155, 1180, 1309, 1442, 2317, 2827
PstI	CTGCAG	1 cut(s)	247
PstNI	CAGNNNCTG	1 cut(s)	383
PsuI	RGATCY	1 cut(s)	2249
PvuII	CAGCTG	2 cut(s)	380, 509
RsaI	GTAC	7 cut(s)	10, 103, 806, 827, 993, 1613, 2291
RsaNI	GTAC	7 cut(s)	9, 102, 805, 826, 992, 1612, 2290
RseI	CAYNNNNRTG	9 cut(s)	594, 779, 800, 881, 1494, 1644, 1764, 2078, 2327
SacI	GAGCTC	1 cut(s)	122
SalI	GTCGAC	1 cut(s)	1226
SaqAI	TTAA	7 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207
Sau96I	GGNCC	6 cut(s)	155, 1180, 1309, 1442, 2317, 2827
SchI	GAGTC	2 cut(s)	2228, 2326
SduI	GDGCHC	8 cut(s)	122, 136, 738, 858, 1206, 1294, 1722, 2647
SfcI	CTRYAG	2 cut(s)	243, 2716
Sfr274I	CTCGAG	1 cut(s)	524
SgrAI	CRCCGGYG	1 cut(s)	659
SinI	GGWCC	3 cut(s)	155, 1309, 1442
SlaI	CTCGAG	1 cut(s)	524
SmiMI	CAYNNNNRTG	9 cut(s)	594, 779, 800, 881, 1494, 1644, 1764, 2078, 2327
SmlI	CTYRAG	5 cut(s)	115, 194, 524, 2551, 2792
SmoI	CTYRAG	5 cut(s)	115, 194, 524, 2551, 2792
SsiI	CCGC	5 cut(s)	64, 287, 820, 980, 1510
SspMI	CTAG	7 cut(s)	143, 167, 1100, 2420, 2519, 2579, 2691
SstI	GAGCTC	1 cut(s)	122
StyI	CCWWGG	3 cut(s)	1445, 1525, 1572
TaaI	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
TaiI	ACGT	1 cut(s)	1378
TaqI	TCGA	7 cut(s)	75, 525, 1041, 1227, 1476, 2025, 2335
TaqII	GACCGA	1 cut(s)	1374
TatI	WGTACW	2 cut(s)	825, 991
TauI	GCSGC	1 cut(s)	289
TfiI	GAWTC	6 cut(s)	67, 217, 569, 1256, 2684, 2807
Tru1I	TTAA	7 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207
Tru9I	TTAA	7 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207
TscAI	CASTG	9 cut(s)	585, 745, 895, 1006, 1809, 1917, 2187, 2817, 2868
TseFI	GTSAC	3 cut(s)	580, 901, 2329
Tsp45I	GTSAC	3 cut(s)	580, 901, 2329
TspDTI	ATGAA	6 cut(s)	1958, 1987, 1993, 2029, 2380, 2543
TspGWI	ACGGA	2 cut(s)	889, 2472
TspRI	CASTG	9 cut(s)	585, 745, 895, 1006, 1809, 1917, 2187, 2817, 2868
VneI	GTGCAC	1 cut(s)	1202
VpaK11BI	GGWCC	3 cut(s)	155, 1309, 1442
XapI	RAATTY	2 cut(s)	188, 2531
XceI	RCATGY	2 cut(s)	1612, 2804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	973
XhoI	CTCGAG	1 cut(s)	524
XmiI	GTMKAC	1 cut(s)	1227
XmnI	GAANNNNTTC	2 cut(s)	573, 1861
XspI	CTAG	7 cut(s)	143, 167, 1100, 2420, 2519, 2579, 2691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.