Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006981

Physical Location & Seq

Genomic Coordinates Reverse (-)

28926967 .. 28934231

Gene Structure 7265 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1254200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2868 bp

ATGGGGAGTACGGACAAGGCCATCAGCCTGGAGGAGATCAAGAACGAGACTGTCGATCTGGAGCGGATTCCGATCGAGGAAGTATTTGGACAGTTGAAATGCTCCAGAGAAGGTTTGAGCTCCGAGGAAGGAGGCCAAAGGCTGGAGATTTTTGGTCCCAACAAGCTAGAAGAGAAAAAGGAAAGCAAATTTCTCAAGTTCCTGGGGTTTATGTGGAATCCTCTATCATGGGTCATGGAAGCTGCAGCCATCATGGCAATTGCTCTGGCCAACGGCGATGGGAAGCCACCAGATTGGCAAGATTTTGTCGGTATTCTCTGCCTGTTGGTCATCAACTCTAGCATCAGTTTTATTGAAGAAAACAATGCTGGAAATGCAGCTGCTGCTCTTATGGCTGGTCTCGCTCCTAAGACTAAGGTCCTTAGAGATGGAAAATGGAGTGAGCAGGATGCTGCCATTTTGGTTCCAGGGGACATCATCAGTATCAAATTGGGAGATATTGTCCCAGCTGATGCTCGTCTTCTTGAGGGTGATCCTTTAAAGATTGATCAATCTGCTCTCACAGGAGAATCACTTCCAGTGACCAAGCATCCAGGTGATGAGGTTTTCTCCGGTTCTACTTGCAAGCAGGGTGAAATTGAAGCTGTTGTTATTGCCACCGGTGTCCATACCTTCTTCGGGAAGGCTGCACATCTTGTGGACAGCACTAACCAAGTCGGACACTTCCAGAAGGTGCTCACTGCCATCGGGAACTTCTGCATCTGTTCTATTGCCATTGGAATGCTGATTGAGATCGTAGTCATGTACCCAATTCAGCACCGCAAGTACCGGAGTGGAATTGACAATCTGTTGGTGCTCTTGATTGGAGGCATCCCCATTGCCATGCCCACTGTCTTGTCTGTTACCATAGCCATTGGTTCTCACAGGCTATCTCAGCAGGGTGCCATCACCAAGAGGATGACTGCCATTGAGGAACTGGCGGGTATGGATGTGCTTTGCAGTGATAAGACAGGAACACTAACTCTTAACAAGCTTAGTGTTGATAAAAACTTGATTGAGGTGTTTGCAAAGGGTGTGGAGAATGAACATGTCATTCTTCTAGCTGCAAGGTCATCCAGAACTGAAAACCAGGATGCCATTGATGCTGCTATGGTTGGAATGCTTGCCGACCCAAAGGAGGCCCGGGCTGGTATTAGAGAGGTGCACTTCTTACCTTTTAATCCCGTCGACAAGAGAACTGCTTTGACATACATTGATAACGATGGAAACTGGCATAGAGCAAGCAAAGGTGCTCCTGAGCAGATTTTGGAACTTTGCAACTGCAAAGAGGATTTCAAGAAGAAGGCTTTTGCCATTATTGACAAGTATGCTGAACGTGGTCTTCGGTCATTGGCTGTTGCTAGACAGGAAGTGCCTGAAAAAGCAAAAGAAAGTGCTGGTGGTCCATGGCAGTTGGTTGGATTGTTGCCTCTTTTCGACCCTCCAAGACATGATAGTGCAGAAACTATCCGCCAGGCTCTTAACCTTGGTGTAAATGTTAAGATGATTACTGGCGATCAACTTGCCATTGCCAAGGAAACTGGTCGGAGACTTGGAATGGGAACAAACATGTACCCATCTGCTTCCTTACTTGGTCAAGGCAAGGATGCTAGCATAGCTGCCCTTCCAATTGAAGAGTTAATTGAGAGGGCTGACGGGTTCGCTGGAGTGTTTCCAGAGCACAAATACGAAATTGTGAAGAAGTTGCAGGAAAAAAAGCACATTTGTGGAATGACGGGAGATGGTGTCAATGATGCCCCTGCATTGAAGAAGGCAGATATTGGAATTGCTGTTGCTGATGCTACAGATGCTGCACGAAGTGCTTCCGACATTGTTCTTACAGAACCCGGATTGAGTGTCATCATTAGTGCAGTGTTGACCAGTAGAGCTATTTTCCAGAGAATGAAGAACTACACAATCTATGCAGTTTCGATCACCATTCGTATTGTGTTCGGTTTCATGTTCATTGCTCTCATATGGAAGTTCGACTTCTCACCTTTCATGGTTTTGATTATTGCCATATTGAACGATGGCACAATTATGACAATATCAAAGGATAGAGTAAAGCCATCTCCCCTGCCTGATAGCTGGAAATTGAAAGAGATTTTTGCTACCGGGATTGTGCTTGGCGGTTACTTGGCATTGATGACTGTTATCTTCTTCTGGTTAATCAAAGAAACTGACTTCTTCTCTGACCAATTTGGTGTAAGATCCTTAAGGGAGACTCCTGAGGAACTAATGGCTGCCTTGTACCTGCAAGTGAGTATTGTAAGCCAGGCCCTCATTTTTGTGACTCGATCCCGCAGCTTCTCCTTTATGGAACGCCCTGGAATGCTTCTACTCGGTGCTTTCTTGATTGCGCAGCTGATTGCAACTTTGATAGCAGTATATGCCAACTGGAGTTTTGCAAGAATACATGGATGTGGTTGGGGATGGGCCGGTGTTATCTGGATTTACAGCATTGTCTTCTATTTCCCACTAGACTTGATGAAATTCGCCATCCGTTACATCTTGAGTGGAAAGGCGTGGCTCAACTTGCTAGAGAACAAGACTGCCTTTACCACCAAGAAAGATTATGGAAAAGAAGAGAGAGAAGCTCAATGGGCTCATGCTCAAAGGACCCTTCACGGTCTCGAAGCACCAGAATCTTCTAGTATCTTCAATGACAAGAGCAGCTACAGAGAGCTGTCTGAGATTGCTGAGCAGGCCAAGAGACGAGCTGAAGTTGCAAGACTTCGGGAGCTTCACACACTCAAGGGACATGTGGAATCAGTTGTGAAGCTAAAAGGCCTGGACATTGACACAATCCAGCAGCATTATACAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

956

Amino Acids

105.01

Weight (kDa)

6.13

Isoelectric Point (pI)

36.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	22 - 85	2.7e-14	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	135 - 233	2.1e-31	P-type ATPase actuator domain
Hydrolase	PF00702	329 - 606	6e-16	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006981)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18960 AT2G18960 AT2G18960 AT4G30190 AT4G30190 AT5G57350 AT5G57350 AT5G57350 AT5G57350
fragaria_vesca	FvH4_4g07810 FvH4_4g07810
malus_domestica	MD13G1247200.v1.1 MD16G1254200.v1.1
prunus_persica	Prupe.1G082000_v2.0.a1
pyrus_communis	pycom12g18290
rosa_chinensis	RchiOBHm_Chr4g0400911
rosa_laevigata	RLG00000009165
rosa_multiflora	Rmu_sc0002026.1_g000039
rosa_roxburghii	Rroxscaffold_5G00345810
rosa_rugosa	Rorug04G0027100
rosa_samantha	Rh4AG104700 Rh4BG099900 Rh4CG113100 Rh4DG096900
rosa_wichuraiana	Rw4G008480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2400
Acc36I	ACCTGC	1 cut(s)	2301
AccB1I	GGYRCC	1 cut(s)	941
AccB7I	CCANNNNNTGG	1 cut(s)	142
AccBSI	CCGCTC	1 cut(s)	64
AccI	GTMKAC	1 cut(s)	1227
AciI	CCGC	6 cut(s)	64, 820, 980, 1510, 2169, 2341
AclWI	GGATC	3 cut(s)	527, 2244, 2331
AcoI	YGGCCR	1 cut(s)	267
AcsI	RAATTY	2 cut(s)	188, 2531
AcuI	CTGAAG	1 cut(s)	2781
AdeI	CACNNNGTG	1 cut(s)	1859
AfaI	GTAC	5 cut(s)	10, 806, 827, 1613, 2291
AfiI	CCNNNNNNNGG	3 cut(s)	142, 678, 1177
AflII	CTTAAG	1 cut(s)	2254
AflIII	ACRYGT	3 cut(s)	1087, 1608, 2800
AgeI	ACCGGT	1 cut(s)	659
AgsI	TTSAA	9 cut(s)	97, 356, 641, 1336, 1673, 1807, 2065, 2137, 2701
AjnI	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
AleI	CACNNNNGTG	1 cut(s)	1764
Alw21I	GWGCWC	6 cut(s)	122, 738, 858, 1206, 1294, 1722
Alw26I	GTCTC	6 cut(s)	41, 404, 1582, 2255, 2675, 2746
Alw44I	GTGCAC	1 cut(s)	1202
AlwI	GGATC	3 cut(s)	527, 2244, 2331
AlwNI	CAGNNNCTG	2 cut(s)	383, 1850
Ama87I	CYCGRG	1 cut(s)	1182
AoxI	GGCC	8 cut(s)	18, 133, 267, 1179, 2316, 2475, 2745, 2827
ApaLI	GTGCAC	1 cut(s)	1202
ApoI	RAATTY	2 cut(s)	188, 2531
AsiGI	ACCGGT	1 cut(s)	659
Asp700I	GAANNNNTTC	2 cut(s)	573, 1861
AspLEI	GCGC	1 cut(s)	2401
AspS9I	GGNCC	7 cut(s)	155, 418, 1180, 1442, 2317, 2475, 2658
AsuC2I	CCSGG	4 cut(s)	1183, 1184, 1887, 2155
AsuHPI	GGTGA	6 cut(s)	542, 608, 644, 940, 1966, 2025
AsuNHI	GCTAGC	1 cut(s)	1649
AvaI	CYCGRG	1 cut(s)	1182
AvaII	GGWCC	4 cut(s)	155, 418, 1442, 2658
AxyI	CCTNAGG	1 cut(s)	2268
BaeGI	GKGCMC	1 cut(s)	1206
BalI	TGGCCA	1 cut(s)	269
BanI	GGYRCC	1 cut(s)	941
BanII	GRGCYC	2 cut(s)	122, 2647
BbsI	GAAGAC	3 cut(s)	512, 1373, 2497
Bbv12I	GWGCWC	6 cut(s)	122, 738, 858, 1206, 1294, 1722
BceAI	ACGGC	1 cut(s)	289
BcgI	CGANNNNNNTGC	2 cut(s)	1544, 1578
BciT130I	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BclI	TGATCA	1 cut(s)	547
BcnI	CCSGG	4 cut(s)	1183, 1184, 1887, 2155
BcoDI	GTCTC	6 cut(s)	41, 404, 1582, 2255, 2675, 2746
BfaI	CTAG	8 cut(s)	167, 339, 1100, 1401, 1650, 2519, 2579, 2691
BfmI	CTRYAG	3 cut(s)	243, 1842, 2716
BfrI	CTTAAG	1 cut(s)	2254
BfuAI	ACCTGC	1 cut(s)	2301
BglI	GCCNNNNNGGC	1 cut(s)	254
BlpI	GCTNAGC	1 cut(s)	2739
Bme18I	GGWCC	4 cut(s)	155, 418, 1442, 2658
BmeT110I	CYCGRG	1 cut(s)	1182
BmgT120I	GGNCC	7 cut(s)	155, 418, 1180, 1442, 2317, 2475, 2658
BmiI	GGNNCC	4 cut(s)	157, 465, 943, 2660
BmtI	GCTAGC	1 cut(s)	1653
BoxI	GACNNNNGTC	1 cut(s)	416
BpiI	GAAGAC	3 cut(s)	512, 1373, 2497
BplI	GAGNNNNNCTC	4 cut(s)	593, 625, 2620, 2652
BpmI	CTGGAG	6 cut(s)	50, 80, 88, 164, 1725, 2458
Bpu10I	CCTNAGC	1 cut(s)	1296
Bpu1102I	GCTNAGC	1 cut(s)	2739
BpuEI	CTTGAG	4 cut(s)	179, 545, 2572, 2777
BpuMI	CCSGG	4 cut(s)	1183, 1184, 1887, 2155
BsaBI	GATNNNNATC	2 cut(s)	71, 791
BsaI	GGTCTC	2 cut(s)	404, 2675
BsaJI	CCNNGG	8 cut(s)	123, 202, 467, 1182, 1445, 1525, 1572, 2365
BsaWI	WCCGGW	3 cut(s)	611, 659, 828
BsaXI	ACNNNNNCTCC	4 cut(s)	486, 516, 1770, 1800
Bsc4I	CCNNNNNNNGG	3 cut(s)	142, 678, 1177
Bse118I	RCCGGY	2 cut(s)	659, 2477
Bse1I	ACTGG	7 cut(s)	578, 981, 1274, 1555, 1585, 1920, 2441
Bse21I	CCTNAGG	1 cut(s)	2268
Bse3DI	GCAATG	3 cut(s)	876, 1566, 2004
Bse8I	GATNNNNATC	2 cut(s)	71, 791
BseBI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BseDI	CCNNGG	8 cut(s)	123, 202, 467, 1182, 1445, 1525, 1572, 2365
BseJI	GATNNNNATC	2 cut(s)	71, 791
BseLI	CCNNNNNNNGG	3 cut(s)	142, 678, 1177
BseMI	GCAATG	3 cut(s)	876, 1566, 2004
BseMII	CTCAG	5 cut(s)	947, 1287, 2259, 2721, 2730
BseNI	ACTGG	7 cut(s)	578, 981, 1274, 1555, 1585, 1920, 2441
BseRI	GAGGAG	1 cut(s)	47
BseSI	GKGCMC	1 cut(s)	1206
BseYI	CCCAGC	1 cut(s)	505
BsgI	GTGCAG	4 cut(s)	672, 1518, 1836, 1929
Bsh1285I	CGRYCG	1 cut(s)	75
BshFI	GGCC	8 cut(s)	20, 135, 269, 1181, 2318, 2477, 2747, 2829
BshNI	GGYRCC	1 cut(s)	941
BshTI	ACCGGT	1 cut(s)	659
BsiEI	CGRYCG	1 cut(s)	75
BsiHKAI	GWGCWC	6 cut(s)	122, 738, 858, 1206, 1294, 1722
BsiHKCI	CYCGRG	1 cut(s)	1182
BsiSI	CCGG	7 cut(s)	612, 660, 829, 1183, 1887, 2154, 2478
BslFI	GGGAC	4 cut(s)	141, 485, 488, 2811
BslI	CCNNNNNNNGG	3 cut(s)	142, 678, 1177
BsmAI	GTCTC	6 cut(s)	41, 404, 1582, 2255, 2675, 2746
BsmBI	CGTCTC	1 cut(s)	2746
BsmFI	GGGAC	4 cut(s)	141, 485, 488, 2811
BsmI	GAATGC	3 cut(s)	786, 1164, 2376
BsnI	GGCC	8 cut(s)	20, 135, 269, 1181, 2318, 2477, 2747, 2829
Bso31I	GGTCTC	2 cut(s)	404, 2675
BsoBI	CYCGRG	1 cut(s)	1182
Bsp1286I	GDGCHC	7 cut(s)	122, 738, 858, 1206, 1294, 1722, 2647
Bsp1720I	GCTNAGC	1 cut(s)	2739
Bsp19I	CCATGG	1 cut(s)	1445
BspACI	CCGC	6 cut(s)	64, 820, 980, 1510, 2169, 2341
BspANI	GGCC	8 cut(s)	20, 135, 269, 1181, 2318, 2477, 2747, 2829
BspCNI	CTCAG	5 cut(s)	946, 1288, 2260, 2722, 2731
BspLI	GGNNCC	4 cut(s)	157, 465, 943, 2660
BspMAI	CTGCAG	1 cut(s)	247
BspMI	ACCTGC	1 cut(s)	2301
BspOI	GCTAGC	1 cut(s)	1653
BspPI	GGATC	3 cut(s)	527, 2244, 2331
BspT107I	GGYRCC	1 cut(s)	941
BspTI	CTTAAG	1 cut(s)	2254
BspTNI	GGTCTC	2 cut(s)	404, 2675
BsrBI	CCGCTC	1 cut(s)	64
BsrDI	GCAATG	3 cut(s)	876, 1566, 2004
BsrFI	RCCGGY	2 cut(s)	659, 2477
BsrI	ACTGG	7 cut(s)	578, 981, 1274, 1555, 1585, 1920, 2441
BssAI	RCCGGY	2 cut(s)	659, 2477
BssECI	CCNNGG	8 cut(s)	123, 202, 467, 1182, 1445, 1525, 1572, 2365
BssT1I	CCWWGG	3 cut(s)	1445, 1525, 1572
Bst2UI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
Bst4CI	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
Bst6I	CTCTTC	3 cut(s)	165, 1668, 2619
BstAFI	CTTAAG	1 cut(s)	2254
BstAPI	GCANNNNNTGC	3 cut(s)	383, 1859, 2429
BstC8I	GCNNGC	5 cut(s)	626, 1164, 1282, 1651, 2745
BstDEI	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
BstDSI	CCRYGG	1 cut(s)	1445
BstHHI	GCGC	1 cut(s)	2401
BstMAI	GTCTC	6 cut(s)	41, 404, 1582, 2255, 2675, 2746
BstMCI	CGRYCG	1 cut(s)	75
BstNI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
BstNSI	RCATGY	3 cut(s)	1091, 1612, 2804
BstPAI	GACNNNNGTC	1 cut(s)	416
BstSFI	CTRYAG	3 cut(s)	243, 1842, 2716
BstSLI	GKGCMC	1 cut(s)	1206
BstV2I	GAAGAC	3 cut(s)	512, 1373, 2497
BstX2I	RGATCY	1 cut(s)	2249
BstXI	CCANNNNNNTGG	2 cut(s)	28, 294
BstYI	RGATCY	1 cut(s)	2249
Bsu36I	CCTNAGG	1 cut(s)	2268
BsuRI	GGCC	8 cut(s)	20, 135, 269, 1181, 2318, 2477, 2747, 2829
BtgI	CCRYGG	1 cut(s)	1445
BtgZI	GCGATG	1 cut(s)	291
BtsI	GCAGTG	3 cut(s)	738, 1006, 1917
BtsIMutI	CAGTG	6 cut(s)	585, 738, 888, 1006, 1917, 2868
BveI	ACCTGC	1 cut(s)	2301
Cac8I	GCNNGC	5 cut(s)	626, 1164, 1282, 1651, 2745
CaiI	CAGNNNCTG	2 cut(s)	383, 1850
CfoI	GCGC	1 cut(s)	2401
Cfr10I	RCCGGY	2 cut(s)	659, 2477
Cfr13I	GGNCC	7 cut(s)	155, 418, 1180, 1442, 2317, 2475, 2658
Cfr9I	CCCGGG	1 cut(s)	1182
Csp6I	GTAC	5 cut(s)	9, 805, 826, 1612, 2290
CspAI	ACCGGT	1 cut(s)	659
CspCI	CAANNNNNGTGG	4 cut(s)	1056, 1091, 2505, 2540
CviQI	GTAC	5 cut(s)	9, 805, 826, 1612, 2290
DdeI	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
DraI	TTTAAA	1 cut(s)	540
DraIII	CACNNNGTG	1 cut(s)	1859
EaeI	YGGCCR	1 cut(s)	267
Eam1104I	CTCTTC	3 cut(s)	165, 1668, 2619
EarI	CTCTTC	3 cut(s)	165, 1668, 2619
EciI	GGCGGA	1 cut(s)	1499
Ecl136II	GAGCTC	1 cut(s)	120
Eco130I	CCWWGG	3 cut(s)	1445, 1525, 1572
Eco147I	AGGCCT	1 cut(s)	2829
Eco24I	GRGCYC	2 cut(s)	122, 2647
Eco31I	GGTCTC	2 cut(s)	404, 2675
Eco47I	GGWCC	4 cut(s)	155, 418, 1442, 2658
Eco53kI	GAGCTC	1 cut(s)	120
Eco57I	CTGAAG	1 cut(s)	2781
Eco81I	CCTNAGG	1 cut(s)	2268
Eco88I	CYCGRG	1 cut(s)	1182
EcoICRI	GAGCTC	1 cut(s)	120
EcoO109I	RGGNCCY	3 cut(s)	418, 2317, 2658
EcoRII	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
EcoT14I	CCWWGG	3 cut(s)	1445, 1525, 1572
EcoT38I	GRGCYC	2 cut(s)	122, 2647
ErhI	CCWWGG	3 cut(s)	1445, 1525, 1572
Esp3I	CGTCTC	1 cut(s)	2746
FalI	AAGNNNNNCTT	4 cut(s)	2012, 2044, 2579, 2611
FaqI	GGGAC	4 cut(s)	141, 485, 488, 2811
FauI	CCCGC	2 cut(s)	973, 2348
FauNDI	CATATG	1 cut(s)	2015
FbaI	TGATCA	1 cut(s)	547
FblI	GTMKAC	1 cut(s)	1227
FriOI	GRGCYC	2 cut(s)	122, 2647
FspBI	CTAG	8 cut(s)	167, 339, 1100, 1401, 1650, 2519, 2579, 2691
FspI	TGCGCA	1 cut(s)	2400
GlaI	GCGC	1 cut(s)	2400
GsaI	CCCAGC	1 cut(s)	509
GsuI	CTGGAG	6 cut(s)	50, 80, 88, 164, 1725, 2458
HaeIII	GGCC	8 cut(s)	20, 135, 269, 1181, 2318, 2477, 2747, 2829
HapII	CCGG	7 cut(s)	612, 660, 829, 1183, 1887, 2154, 2478
HhaI	GCGC	1 cut(s)	2401
Hin6I	GCGC	1 cut(s)	2399
HinP1I	GCGC	1 cut(s)	2399
HincII	GTYRAC	2 cut(s)	1228, 1917
HindII	GTYRAC	2 cut(s)	1228, 1917
HindIII	AAGCTT	1 cut(s)	1031
HinfI	GANTC	7 cut(s)	67, 217, 569, 2263, 2332, 2684, 2807
HpaII	CCGG	7 cut(s)	612, 660, 829, 1183, 1887, 2154, 2478
HphI	GGTGA	6 cut(s)	542, 608, 644, 940, 1966, 2025
Hpy166II	GTNNAC	4 cut(s)	700, 1204, 1228, 1917
Hpy188I	TCNGA	7 cut(s)	72, 124, 719, 1587, 1867, 2233, 2731
Hpy8I	GTNNAC	4 cut(s)	700, 1204, 1228, 1917
Hpy99I	CGWCG	1 cut(s)	1229
HpyAV	CCTTC	9 cut(s)	104, 122, 676, 682, 724, 1336, 1673, 1804, 2672
HpyCH4III	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
HpyCH4IV	ACGT	1 cut(s)	1375
HpyF3I	CTNAG	9 cut(s)	408, 414, 422, 933, 1034, 1296, 2268, 2730, 2739
HpySE526I	ACGT	1 cut(s)	1375
HspAI	GCGC	1 cut(s)	2399
Ksp22I	TGATCA	1 cut(s)	547
LmnI	GCTCC	6 cut(s)	61, 107, 125, 409, 1297, 2779
MaeI	CTAG	8 cut(s)	167, 339, 1100, 1401, 1650, 2519, 2579, 2691
MaeII	ACGT	1 cut(s)	1375
MaeIII	GTNAC	5 cut(s)	580, 901, 2171, 2329, 2543
MbiI	CCGCTC	1 cut(s)	64
MfeI	CAATTG	2 cut(s)	258, 1668
MflI	RGATCY	1 cut(s)	2249
MhlI	GDGCHC	7 cut(s)	122, 738, 858, 1206, 1294, 1722, 2647
MlsI	TGGCCA	1 cut(s)	269
MluNI	TGGCCA	1 cut(s)	269
MlyI	GAGTC	2 cut(s)	2257, 2326
MmeI	TCCRAC	5 cut(s)	697, 1135, 1438, 1565, 1890
Mox20I	TGGCCA	1 cut(s)	269
MroXI	GAANNNNTTC	2 cut(s)	573, 1861
MscI	TGGCCA	1 cut(s)	269
MseI	TTAA	8 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207, 2255
MslI	CAYNNNNRTG	8 cut(s)	594, 779, 881, 1494, 1764, 2078, 2327, 2460
Msp20I	TGGCCA	1 cut(s)	269
MspA1I	CMGCKG	3 cut(s)	380, 509, 2404
MspCI	CTTAAG	1 cut(s)	2254
MspI	CCGG	7 cut(s)	612, 660, 829, 1183, 1887, 2154, 2478
MunI	CAATTG	2 cut(s)	258, 1668
Mva1269I	GAATGC	3 cut(s)	786, 1164, 2376
MvaI	CCWGG	9 cut(s)	29, 203, 468, 594, 1130, 1514, 2315, 2367, 2831
NciI	CCSGG	4 cut(s)	1183, 1184, 1887, 2155
NcoI	CCATGG	1 cut(s)	1445
NdeI	CATATG	1 cut(s)	2015
NheI	GCTAGC	1 cut(s)	1649
NlaIV	GGNNCC	4 cut(s)	157, 465, 943, 2660
NmuCI	GTSAC	2 cut(s)	580, 2329
NsbI	TGCGCA	1 cut(s)	2400
NspI	RCATGY	3 cut(s)	1091, 1612, 2804
OliI	CACNNNNGTG	1 cut(s)	1764
PceI	AGGCCT	1 cut(s)	2829
PciI	ACATGT	3 cut(s)	1087, 1608, 2800
PcsI	WCGNNNNNNNCGW	1 cut(s)	51
PctI	GAATGC	3 cut(s)	786, 1164, 2376
PdmI	GAANNNNTTC	2 cut(s)	573, 1861
PfeI	GAWTC	5 cut(s)	67, 217, 569, 2684, 2807
PflMI	CCANNNNNTGG	1 cut(s)	142
PinAI	ACCGGT	1 cut(s)	659
Ple19I	CGATCG	1 cut(s)	75
PleI	GAGTC	2 cut(s)	2257, 2326
PpsI	GAGTC	2 cut(s)	2257, 2326
PpuMI	RGGWCCY	2 cut(s)	418, 2658
PscI	ACATGT	3 cut(s)	1087, 1608, 2800
PshAI	GACNNNNGTC	1 cut(s)	416
Psp124BI	GAGCTC	1 cut(s)	122
Psp5II	RGGWCCY	2 cut(s)	418, 2658
Psp6I	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
PspFI	CCCAGC	1 cut(s)	505
PspGI	CCWGG	9 cut(s)	27, 201, 466, 592, 1128, 1512, 2313, 2365, 2829
PspN4I	GGNNCC	4 cut(s)	157, 465, 943, 2660
PspPI	GGNCC	7 cut(s)	155, 418, 1180, 1442, 2317, 2475, 2658
PspPPI	RGGWCCY	2 cut(s)	418, 2658
PstI	CTGCAG	1 cut(s)	247
PstNI	CAGNNNCTG	2 cut(s)	383, 1850
PsuI	RGATCY	1 cut(s)	2249
PvuI	CGATCG	1 cut(s)	75
PvuII	CAGCTG	3 cut(s)	380, 509, 2404
RsaI	GTAC	5 cut(s)	10, 806, 827, 1613, 2291
RsaNI	GTAC	5 cut(s)	9, 805, 826, 1612, 2290
RseI	CAYNNNNRTG	8 cut(s)	594, 779, 881, 1494, 1764, 2078, 2327, 2460
SacI	GAGCTC	1 cut(s)	122
SalI	GTCGAC	1 cut(s)	1226
SaqAI	TTAA	8 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207, 2255
Sau96I	GGNCC	7 cut(s)	155, 418, 1180, 1442, 2317, 2475, 2658
SchI	GAGTC	2 cut(s)	2257, 2326
SduI	GDGCHC	7 cut(s)	122, 738, 858, 1206, 1294, 1722, 2647
SfcI	CTRYAG	3 cut(s)	243, 1842, 2716
SgrAI	CRCCGGYG	1 cut(s)	659
SinI	GGWCC	4 cut(s)	155, 418, 1442, 2658
SmaI	CCCGGG	1 cut(s)	1184
SmiMI	CAYNNNNRTG	8 cut(s)	594, 779, 881, 1494, 1764, 2078, 2327, 2460
SmlI	CTYRAG	5 cut(s)	194, 524, 2254, 2551, 2792
SmoI	CTYRAG	5 cut(s)	194, 524, 2254, 2551, 2792
SrfI	GCCCGGGC	1 cut(s)	1184
SseBI	AGGCCT	1 cut(s)	2829
SsiI	CCGC	6 cut(s)	64, 820, 980, 1510, 2169, 2341
SspMI	CTAG	8 cut(s)	167, 339, 1100, 1401, 1650, 2519, 2579, 2691
SstI	GAGCTC	1 cut(s)	122
StuI	AGGCCT	1 cut(s)	2829
StyI	CCWWGG	3 cut(s)	1445, 1525, 1572
TaaI	ACNGT	6 cut(s)	52, 93, 892, 2191, 2669, 2863
TaiI	ACGT	1 cut(s)	1378
TaqI	TCGA	8 cut(s)	54, 75, 1227, 1476, 1970, 2025, 2335, 2673
TaqII	GACCGA	1 cut(s)	1374
TfiI	GAWTC	5 cut(s)	67, 217, 569, 2684, 2807
Tru1I	TTAA	8 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207, 2255
Tru9I	TTAA	8 cut(s)	539, 1026, 1218, 1521, 1539, 1679, 2207, 2255
TscAI	CASTG	6 cut(s)	585, 745, 895, 1006, 1917, 2868
TseFI	GTSAC	2 cut(s)	580, 2329
Tsp45I	GTSAC	2 cut(s)	580, 2329
TspDTI	ATGAA	6 cut(s)	1098, 1958, 1987, 1993, 2029, 2543
TspGWI	ACGGA	2 cut(s)	26, 2531
TspMI	CCCGGG	1 cut(s)	1182
TspRI	CASTG	6 cut(s)	585, 745, 895, 1006, 1917, 2868
Van91I	CCANNNNNTGG	1 cut(s)	142
Vha464I	CTTAAG	1 cut(s)	2254
VneI	GTGCAC	1 cut(s)	1202
VpaK11BI	GGWCC	4 cut(s)	155, 418, 1442, 2658
XapI	RAATTY	2 cut(s)	188, 2531
XceI	RCATGY	3 cut(s)	1091, 1612, 2804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	973
XmaI	CCCGGG	1 cut(s)	1182
XmiI	GTMKAC	1 cut(s)	1227
XmnI	GAANNNNTTC	2 cut(s)	573, 1861
XspI	CTAG	8 cut(s)	167, 339, 1100, 1401, 1650, 2519, 2579, 2691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.