MD14G1009500.v1.1
ERF Family

DnaJ Hsp40 cysteine-rich domain superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014243
Physical Location & Seq
Forward (+)
948945 .. 956259
7315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1009500.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGGTTCTGCTTCTGCTGCTGCAAATACGGCGTCTTTGATTACTGTCGTCCCTGTTTCTTCGACAAAGTTGGATATCCATGACAAATTTTCATATGGGTTTCATCTCAGGCGTGGTGGGTCGATCGCCAAATTTACTGCCGCCATCTCTCCCAACGGCTCTGTTTCTCCTTCTTCTTCACATGGGGCTGGGTGCTCAGCTGGAGATGCGGATAAACAAAGAAGTAGTCTTGAATCTCTGTTTTGTTATGATAAGTCTGTACCGGAAGAGAGAATCGAGAAGCCTATCGGGATATCTTTGCCTGAGAAAATCATTGGAAACAATCCCCGATGCTTTGACTGTGAAGCTAAAGGCGCAGTCCTTTGCACCACTTGTTCTGGTTCAGGCCTGTATGTTGATTCAGTACTGGAGAGCCAGGGTATTCTTGTCAACATTCGCTGCCTCGGTTGTGGGGGAAGTGGCAAGATTATGTGTTCAGAATGTGGTGGGCGCGGGCATCTAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

17.25

Weight (kDa)

7.52

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17840
fragaria_vesca FvH4_1g29880
malus_domestica MD12G1011900.v1.1 MD14G1009500.v1.1
prunus_persica Prupe.7G015800_v2.0.a1
pyrus_communis pycom12g00920
rosa_chinensis RchiOBHm_Chr3g0496031
rosa_laevigata RLG00000022571
rosa_multiflora Rmu_sc0000607.1_g000055 Rmu_sc0005781.1_g000028
rosa_roxburghii Rroxscaffold_6G00389640
rosa_rugosa Rorug03G0279200
rosa_samantha Rh3AG325900 Rh3BG362300 Rh3CG358300 Rh3DG361700
rosa_wichuraiana Rw3G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 492
AciI CCGC 3 cut(s) 141, 209, 492
AcsI RAATTY 2 cut(s) 86, 131
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 261, 405
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 2 cut(s) 200, 347
AluI AGCT 2 cut(s) 200, 347
Alw21I GWGCWC 1 cut(s) 197
AoxI GGCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 17, 20, 438
ApoI RAATTY 2 cut(s) 86, 131
ArsI GACNNNNNNTTYG 4 cut(s) 17, 49, 211, 243
AspLEI GCGC 2 cut(s) 356, 492
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 3 cut(s) 4, 7, 425
BccI CCATC 1 cut(s) 152
BceAI ACGGC 2 cut(s) 45, 172
BciT130I CCWGG 1 cut(s) 416
BfaI CTAG 1 cut(s) 500
BisI GCNGC 4 cut(s) 18, 21, 141, 439
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 4 cut(s) 19, 22, 142, 440
BmcAI AGTACT 1 cut(s) 405
Bme1390I CCNGG 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 2 cut(s) 196, 320
BpmI CTGGAG 2 cut(s) 222, 428
Bpu1102I GCTNAGC 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 415, 442
BsaWI WCCGGW 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 2 cut(s) 415, 442
BseMII CTCAG 3 cut(s) 121, 210, 294
BseNI ACTGG 1 cut(s) 411
BseXI GCAGC 3 cut(s) 4, 7, 425
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 492
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 1 cut(s) 123
Bsp1720I GCTNAGC 1 cut(s) 196
BspACI CCGC 3 cut(s) 141, 209, 492
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 3 cut(s) 120, 209, 295
BspFNI CGCG 1 cut(s) 492
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 2 cut(s) 415, 442
BssMI GATC 1 cut(s) 123
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 46, 341
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 3 cut(s) 107, 196, 303
BstFNI CGCG 1 cut(s) 492
BstHHI GCGC 2 cut(s) 356, 492
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 4 cut(s) 17, 29, 206, 353
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 4, 7, 425
BsuRI GGCC 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 494
CfoI GCGC 2 cut(s) 356, 492
CseI GACGC 1 cut(s) 21
Csp6I GTAC 2 cut(s) 260, 404
CviAII CATG 2 cut(s) 80, 182
CviJI RGCY 7 cut(s) 159, 188, 200, 283, 347, 387, 414
CviKI_1 RGCY 7 cut(s) 159, 188, 200, 283, 347, 387, 414
CviQI GTAC 2 cut(s) 260, 404
DdeI CTNAG 3 cut(s) 107, 196, 303
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 387
Eco32I GATATC 2 cut(s) 76, 294
EcoRII CCWGG 1 cut(s) 414
EcoRV GATATC 2 cut(s) 76, 294
FaeI CATG 2 cut(s) 83, 185
FaiI YATR 7 cut(s) 81, 94, 96, 183, 249, 393, 470
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 79, 181
FauI CCCGC 1 cut(s) 485
FauNDI CATATG 1 cut(s) 94
Fnu4HI GCNGC 4 cut(s) 18, 21, 141, 439
Fsp4HI GCNGC 4 cut(s) 18, 21, 141, 439
FspBI CTAG 1 cut(s) 500
GlaI GCGC 2 cut(s) 355, 491
GluI GCNGC 4 cut(s) 18, 21, 141, 439
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 2 cut(s) 222, 428
HaeIII GGCC 1 cut(s) 387
HapII CCGG 1 cut(s) 263
HgaI GACGC 1 cut(s) 21
HhaI GCGC 2 cut(s) 356, 492
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 2 cut(s) 83, 185
Hin6I GCGC 2 cut(s) 354, 490
HinP1I GCGC 2 cut(s) 354, 490
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 3 cut(s) 233, 273, 398
HpaII CCGG 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 1 cut(s) 478
Hpy188III TCNNGA 3 cut(s) 230, 277, 289
Hpy8I GTNNAC 1 cut(s) 430
HpyAV CCTTC 1 cut(s) 180
HpyCH4III ACNGT 2 cut(s) 46, 341
HpyCH4V TGCA 2 cut(s) 23, 366
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 29, 206, 353
HpyF3I CTNAG 3 cut(s) 107, 196, 303
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 2 cut(s) 83, 185
HspAI GCGC 2 cut(s) 354, 490
Kzo9I GATC 1 cut(s) 123
Lsp1109I GCAGC 3 cut(s) 4, 7, 425
LweI GCATC 2 cut(s) 196, 320
MaeI CTAG 1 cut(s) 500
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 4 cut(s) 51, 165, 168, 278
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 2 cut(s) 86, 131
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 4 cut(s) 17, 29, 206, 353
NdeI CATATG 1 cut(s) 94
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 83, 185
PceI AGGCCT 1 cut(s) 387
PfeI GAWTC 3 cut(s) 233, 273, 398
PkrI GCNGC 4 cut(s) 19, 22, 142, 440
Ple19I CGATCG 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 1 cut(s) 414
PvuI CGATCG 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 2 cut(s) 261, 405
RsaNI GTAC 2 cut(s) 260, 404
SatI GCNGC 4 cut(s) 18, 21, 141, 439
Sau3AI GATC 1 cut(s) 123
ScaI AGTACT 1 cut(s) 405
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 197
SetI ASST 2 cut(s) 202, 349
SfaNI GCATC 2 cut(s) 196, 320
Sse9I AATT 2 cut(s) 86, 131
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 3 cut(s) 141, 209, 492
SspMI CTAG 1 cut(s) 500
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 46, 341
TaqI TCGA 3 cut(s) 62, 122, 276
TasI AATT 2 cut(s) 86, 131
TatI WGTACW 1 cut(s) 403
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 233, 273, 398
TseI GCWGC 3 cut(s) 17, 20, 438
TspDTI ATGAA 2 cut(s) 81, 92
XapI RAATTY 2 cut(s) 86, 131
XspI CTAG 1 cut(s) 500
ZrmI AGTACT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.