RLG00000022571
ERF Family

DnaJ Hsp40 cysteine-rich domain superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014243
Physical Location & Seq
Forward (+)
4068432 .. 4070843
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022571

Sequence Viewer

Length: 504 bp
ATGGGTTCTGCTGCTGCTCTGAATATGGCGTCTTCCATTACTGTAATCAGCTCAGCTTGTTCGACGAAATCAAAGCTCAATGACAAATTCACCTGCGGGTTTCATAAATTCAATCCCATCACCAAAATCTGTGCCATATCTCCAAACGGTTCCGTTTCTGGTTTTGCTTCTGCTTCACACGGGGCTGAGTGCTCAGCTGGATACGTGCATAAACAAAGAAGTAGTCTTGAATCTTTTTTTTGTTATGATAAGGCTATTCCGGAGGAGATTATTGAGAAGCCCATAGGGATATCTTTGGTGGAAAAATTCATTGGAAACAATCCCCGATGCATAGATTGTGAAGCAAAAGGCGCTGTCCTCTGCACAACTTGCTCTGGATCAGGCCTGTATGTTGATTCAATACTGGAGAGCCAGGGCATTATTGTCAAAGTCCGCTGCCTAGGTTGTGGGGGAACTGGTAACATTATGTGTTCAGAGTGTGGTGGCCGCGGTCATCGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

17.37

Weight (kDa)

8.22

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17840
fragaria_vesca FvH4_1g29880
malus_domestica MD12G1011900.v1.1 MD14G1009500.v1.1
prunus_persica Prupe.7G015800_v2.0.a1
pyrus_communis pycom12g00920
rosa_chinensis RchiOBHm_Chr3g0496031
rosa_laevigata RLG00000022571
rosa_multiflora Rmu_sc0000607.1_g000055 Rmu_sc0005781.1_g000028
rosa_roxburghii Rroxscaffold_6G00389640
rosa_rugosa Rorug03G0279200
rosa_samantha Rh3AG325900 Rh3BG362300 Rh3CG358300 Rh3DG361700
rosa_wichuraiana Rw3G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 101
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 4 cut(s) 96, 433, 487, 489
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 3 cut(s) 86, 107, 305
AcyI GRCGYC 1 cut(s) 29
AgsI TTSAA 3 cut(s) 112, 230, 399
AjnI CCWGG 1 cut(s) 411
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AluBI AGCT 4 cut(s) 51, 56, 76, 197
AluI AGCT 4 cut(s) 51, 56, 76, 197
Alw21I GWGCWC 1 cut(s) 194
AlwI GGATC 1 cut(s) 385
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 2 cut(s) 382, 484
ApeKI GCWGC 3 cut(s) 11, 14, 435
ApoI RAATTY 3 cut(s) 86, 107, 305
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 1 cut(s) 353
AsuHPI GGTGA 2 cut(s) 82, 112
AvrII CCTAGG 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 422
BccI CCATC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 413
BciVI GTATCC 1 cut(s) 194
BfaI CTAG 1 cut(s) 440
BfoI RGCGCY 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 4 cut(s) 12, 15, 436, 487
BlnI CCTAGG 1 cut(s) 439
BlpI GCTNAGC 2 cut(s) 52, 193
BlsI GCNGC 4 cut(s) 13, 16, 437, 488
Bme1390I CCNGG 1 cut(s) 413
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 425
Bpu1102I GCTNAGC 2 cut(s) 52, 193
BsaAI YACGTR 1 cut(s) 205
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 412, 439, 487
BsaWI WCCGGW 1 cut(s) 259
Bse1I ACTGG 2 cut(s) 408, 460
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 3 cut(s) 412, 439, 487
BseMII CTCAG 3 cut(s) 66, 177, 207
BseNI ACTGG 2 cut(s) 408, 460
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 384, 486
BsiHKAI GWGCWC 1 cut(s) 194
BsiSI CCGG 1 cut(s) 260
BsnI GGCC 2 cut(s) 384, 486
Bsp1286I GDGCHC 1 cut(s) 194
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 1 cut(s) 377
Bsp1720I GCTNAGC 2 cut(s) 52, 193
BspACI CCGC 4 cut(s) 96, 433, 487, 489
BspANI GGCC 2 cut(s) 384, 486
BspCNI CTCAG 3 cut(s) 65, 178, 206
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 1 cut(s) 489
BspLI GGNNCC 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 385
BsrI ACTGG 2 cut(s) 408, 460
BssECI CCNNGG 3 cut(s) 412, 439, 487
BssMI GATC 1 cut(s) 377
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 2 cut(s) 43, 149
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 369
BstBAI YACGTR 1 cut(s) 205
BstDEI CTNAG 3 cut(s) 52, 186, 193
BstDSI CCRYGG 1 cut(s) 487
BstFNI CGCG 1 cut(s) 489
BstH2I RGCGCY 1 cut(s) 354
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 1 cut(s) 380
BstMBI GATC 1 cut(s) 377
BstMWI GCNNNNNNNGC 2 cut(s) 350, 369
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 411
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 422
BstV2I GAAGAC 1 cut(s) 24
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 2 cut(s) 384, 486
BtgI CCRYGG 1 cut(s) 487
BveI ACCTGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 353
Cfr42I CCGCGG 1 cut(s) 490
CseI GACGC 1 cut(s) 18
DdeI CTNAG 3 cut(s) 52, 186, 193
DpnI GATC 1 cut(s) 379
DpnII GATC 1 cut(s) 377
EaeI YGGCCR 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 439
Eco147I AGGCCT 1 cut(s) 384
Eco32I GATATC 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 411
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 439
EcoT22I ATGCAT 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 439
FaiI YATR 9 cut(s) 26, 105, 137, 210, 246, 284, 332, 390, 467
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 4 cut(s) 12, 15, 436, 487
Fsp4HI GCNGC 4 cut(s) 12, 15, 436, 487
FspBI CTAG 1 cut(s) 440
GlaI GCGC 1 cut(s) 352
GluI GCNGC 4 cut(s) 12, 15, 436, 487
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 354
HaeIII GGCC 2 cut(s) 384, 486
HapII CCGG 1 cut(s) 260
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 353
Hin1I GRCGYC 1 cut(s) 29
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 2 cut(s) 230, 395
HpaII CCGG 1 cut(s) 260
HphI GGTGA 2 cut(s) 82, 112
Hpy188I TCNGA 2 cut(s) 21, 475
Hpy188III TCNNGA 3 cut(s) 227, 260, 375
Hpy99I CGWCG 1 cut(s) 67
HpyCH4III ACNGT 2 cut(s) 43, 149
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 3 cut(s) 208, 330, 363
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 369
HpyF3I CTNAG 3 cut(s) 52, 186, 193
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 29
HspAI GCGC 1 cut(s) 351
Kpn2I TCCGGA 1 cut(s) 259
KspI CCGCGG 1 cut(s) 490
Kzo9I GATC 1 cut(s) 377
Lsp1109I GCAGC 1 cut(s) 422
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 440
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 458
MalI GATC 1 cut(s) 379
MboI GATC 1 cut(s) 377
MboII GAAGA 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 3 cut(s) 86, 107, 305
MnlI CCTC 2 cut(s) 256, 368
Mph1103I ATGCAT 1 cut(s) 332
MroI TCCGGA 1 cut(s) 259
MspA1I CMGCKG 3 cut(s) 197, 435, 489
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 350, 369
NdeII GATC 1 cut(s) 377
NlaIV GGNNCC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 332
PaqCI CACCTGC 1 cut(s) 101
PceI AGGCCT 1 cut(s) 384
PfeI GAWTC 2 cut(s) 230, 395
PkrI GCNGC 4 cut(s) 13, 16, 437, 488
Ppu21I YACGTR 1 cut(s) 205
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 197
SacII CCGCGG 1 cut(s) 490
SatI GCNGC 4 cut(s) 12, 15, 436, 487
Sau3AI GATC 1 cut(s) 377
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 1 cut(s) 194
SetI ASST 7 cut(s) 53, 58, 78, 95, 199, 207, 445
SfaNI GCATC 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 490
SgrBI CCGCGG 1 cut(s) 490
Sse9I AATT 3 cut(s) 86, 107, 305
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 4 cut(s) 96, 433, 487, 489
SspMI CTAG 1 cut(s) 440
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 2 cut(s) 43, 149
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 62
TasI AATT 3 cut(s) 86, 107, 305
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 2 cut(s) 230, 395
TseI GCWGC 3 cut(s) 11, 14, 435
TspDTI ATGAA 2 cut(s) 92, 298
TspGWI ACGGA 1 cut(s) 142
XapI RAATTY 3 cut(s) 86, 107, 305
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 1 cut(s) 440
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.