MD14G1019500.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016884
Physical Location & Seq
Reverse (-)
1833021 .. 1834559
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1019500.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGTCCCATTCCTTGATTATATTTCCAATGGAACTTCAAGTTTCTGAGCTCTTGCAATTCTTTTACACCTTCCTTTTCTTTCTTCTATTCTCCATCTTCAGCCTTTTCCTATTGAGATCAAACCGATGGTGCAGTTGTGAAATTTGCCAGACCTATGTCACCTCAAGCTGGTCTTTAAAATTCATAAATCTTTGTGATTGGTATGCACATCTTCTTAGAGATTCTCCAAGCAAAACCATCCACATACATGTTCTAAAAAACACCATCACAGCCAACCCTGAAAACGTCGAGTACATGCTCAAAACTCGATTTGAAAACTACCCGAAAGGCAAACCCTTCTCTACAATCCTTGGTGACTTTCTAGGTCAAGGCATATTCAACGTGGATGGCCACTCATGGAGGTTCCAGAGGAAGATGGCAAGCCTCGAGCTCGACAGACACTCGGTACGATCGTATGCTTACGAGATTGTCAATCACGAAATCGAACACCGTCTCATCCCTCTTTTATCATCCGTTTGTTCTAATTCGGATGATGGTAACGGAGTATTCCTCGATTTGCAAGACGTGTTCAAGAGATTTTCGTTCGACAGCATATGCAAGTTCTCTTTCGGGTTGGACCCTATGTGCCTAGAGTTGTCACTGCCCTTGTCCAAATTCACCATCGCCTTCGACATGGCCTCGAAGTTGTCCGCCGAGAGAGCCATGACGCCATCTCCCATAATTTGGAAGATCAAGAGAATTTTAAATTTGGGGAGCGAGAAGCAACTAAAAGAATCCATAAACATCATCGACGGTTTAGCCAAAGAGGTGATCGCCAGAAAGCGCGAAGTGGGATTTTCAACCCAAAAAGATCTCTTGTCAAGATTCATGAGAAGTGTAGAAGATGAATCCTACTTGAGAGACATTGTTGTAAGTTTTTTATTGGCTGGGCGTGACACTGTTGCGTCCGCTTTGACAAGTTTCTTTTGGCTCATTGGGACCCACCCGAACGTTGCGTCAGCGATTCGGTTGGAAGCTGATAGAGTGCTTGGACTCAATCAGGGCCTCAAGAGCTTTGATCAAATGCAAGAGCTTCACTATTTACAATCCGCAATTTATGAGAGCATGAGGCTCTACCCTCCTGTACAGTTTGACTCAAAGTTTTGTGAACAAGATGATGTTTTACCCGATGGCACATTTGTGAGGAAAGGGACTAGGGTTACTTACCATCCGTACGCAATGGGTCGGATCCAGGAGATTTGGGGCTCTGACTGCATGGAATTTAAGCCCGACAGATGGTTGAAGGATGGCGTTTTCTTCAAGGAAAACCCCTTCCAGTACCCAGTGTTTCAACCGGGACATAGGGTTTGTCTGGGGAAAGAGATGGCTGTGATGGAGATGAAAACCGTTGTGCTTTCGCTGCTCAGACGATTTAACATCGAACATGCAGCACACGAATCAAGTAGTGTGCCTCGGTTCTCTCCCGGACTCACTGCGACCTTAAGCGGTGGGCTTCCAGTGCTGGTGAAAGAAAGGACTAGCACCACCATCGAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

513

Amino Acids

58.83

Weight (kDa)

7.21

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 89 - 476 3e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016884)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g18280
malus_domestica MD12G1022700.v1.1 MD14G1019500.v1.1
prunus_persica Prupe.7G114100_v2.0.a1
pyrus_communis pycom12g02100
rosa_chinensis RchiOBHm_Chr3g0472721
rosa_laevigata RLG00000024060
rosa_multiflora Rmu_co8458407.1_g000001
rosa_rugosa Rorug03G0127600
rosa_samantha Rh3AG180000 Rh3BG206100 Rh3CG203100 Rh3DG169600
rosa_wichuraiana Rw3G016260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 723
AccII CGCG 1 cut(s) 825
AciI CCGC 4 cut(s) 690, 948, 1089, 1485
AclI AACGTT 1 cut(s) 990
AclWI GGATC 2 cut(s) 1222, 1235
AcoI YGGCCR 1 cut(s) 388
AcsI RAATTY 6 cut(s) 141, 179, 653, 738, 745, 1259
AcuI CTGAAG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 707
AfaI GTAC 5 cut(s) 293, 447, 1125, 1214, 1319
AfiI CCNNNNNNNGG 3 cut(s) 168, 723, 1275
AflII CTTAAG 1 cut(s) 1480
AflIII ACRYGT 2 cut(s) 247, 564
AgsI TTSAA 8 cut(s) 38, 314, 379, 571, 840, 1282, 1300, 1331
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 1230
AleI CACNNNNGTG 1 cut(s) 1178
AluBI AGCT 6 cut(s) 49, 168, 430, 1016, 1053, 1072
AluI AGCT 6 cut(s) 49, 168, 430, 1016, 1053, 1072
Alw21I GWGCWC 2 cut(s) 51, 432
Alw26I GTCTC 2 cut(s) 497, 894
AlwI GGATC 2 cut(s) 1222, 1235
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 3 cut(s) 388, 675, 1042
ApeKI GCWGC 2 cut(s) 1399, 1427
ApoI RAATTY 6 cut(s) 141, 179, 653, 738, 745, 1259
ArsI GACNNNNNNTTYG 4 cut(s) 980, 1012, 1329, 1361
AspLEI GCGC 1 cut(s) 825
AspS9I GGNCC 3 cut(s) 616, 978, 1042
AsuC2I CCSGG 2 cut(s) 1335, 1464
AsuHPI GGTGA 5 cut(s) 151, 365, 649, 820, 1516
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 616, 978
BaeI ACNNNNGTAYC 2 cut(s) 437, 470
BalI TGGCCA 1 cut(s) 390
BamHI GGATCC 1 cut(s) 1227
BanII GRGCYC 3 cut(s) 51, 432, 1247
Bbv12I GWGCWC 2 cut(s) 51, 432
BbvI GCAGC 2 cut(s) 1386, 1439
BcgI CGANNNNNNTGC 1 cut(s) 1510
BciT130I CCWGG 1 cut(s) 1232
BclI TGATCA 1 cut(s) 1057
BcnI CCSGG 2 cut(s) 1335, 1464
BcoDI GTCTC 2 cut(s) 497, 894
BfaI CTAG 4 cut(s) 362, 629, 1194, 1518
BfrI CTTAAG 1 cut(s) 1480
BglII AGATCT 1 cut(s) 850
BisI GCNGC 2 cut(s) 1400, 1428
BlsI GCNGC 2 cut(s) 1401, 1429
Bme1390I CCNGG 3 cut(s) 1232, 1335, 1464
Bme18I GGWCC 2 cut(s) 616, 978
BmeT110I CYCGRG 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 3 cut(s) 616, 978, 1042
BmiI GGNNCC 5 cut(s) 404, 618, 979, 980, 1229
BmrFI CCNGG 3 cut(s) 1232, 1335, 1464
BmrI ACTGGG 1 cut(s) 1316
BmuI ACTGGG 1 cut(s) 1316
BoxI GACNNNNGTC 1 cut(s) 155
BplI GAGNNNNNCTC 2 cut(s) 534, 566
BpuEI CTTGAG 3 cut(s) 148, 916, 1031
BpuMI CCSGG 2 cut(s) 1335, 1464
BsaHI GRCGYC 1 cut(s) 707
BsaJI CCNNGG 2 cut(s) 349, 1451
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 723, 1275
Bse1I ACTGG 3 cut(s) 1315, 1322, 1496
Bse3DI GCAATG 1 cut(s) 1224
BseBI CCWGG 1 cut(s) 1232
BseDI CCNNGG 2 cut(s) 349, 1451
BseGI GGATG 7 cut(s) 237, 391, 495, 509, 535, 1207, 1291
BseLI CCNNNNNNNGG 3 cut(s) 168, 723, 1275
BseMI GCAATG 1 cut(s) 1224
BseMII CTCAG 2 cut(s) 36, 1417
BseNI ACTGG 3 cut(s) 1315, 1322, 1496
BseXI GCAGC 2 cut(s) 1386, 1439
BseYI CCCAGC 1 cut(s) 926
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 452
BshFI GGCC 3 cut(s) 390, 677, 1044
BsiEI CGRYCG 1 cut(s) 452
BsiHKAI GWGCWC 2 cut(s) 51, 432
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 2 cut(s) 1334, 1464
BsiWI CGTACG 1 cut(s) 1212
BslFI GGGAC 3 cut(s) 991, 1204, 1350
BslI CCNNNNNNNGG 3 cut(s) 168, 723, 1275
BsmAI GTCTC 2 cut(s) 497, 894
BsmBI CGTCTC 1 cut(s) 497
BsmFI GGGAC 3 cut(s) 991, 1204, 1350
BsnI GGCC 3 cut(s) 390, 677, 1044
BsoBI CYCGRG 1 cut(s) 425
Bsp1286I GDGCHC 3 cut(s) 51, 432, 1247
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
BspACI CCGC 4 cut(s) 690, 948, 1089, 1485
BspANI GGCC 3 cut(s) 390, 677, 1044
BspCNI CTCAG 2 cut(s) 37, 1416
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 2 cut(s) 867, 1535
BspLI GGNNCC 5 cut(s) 404, 618, 979, 980, 1229
BspPI GGATC 2 cut(s) 1222, 1235
BspTI CTTAAG 1 cut(s) 1480
BsrDI GCAATG 1 cut(s) 1224
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 3 cut(s) 1315, 1322, 1496
BssECI CCNNGG 2 cut(s) 349, 1451
BssMI GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
BssNI GRCGYC 1 cut(s) 707
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 1232
Bst4CI ACNGT 5 cut(s) 491, 794, 940, 1128, 1387
BstACI GRCGYC 1 cut(s) 707
BstAFI CTTAAG 1 cut(s) 1480
BstAUI TGTACA 1 cut(s) 1123
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 3 cut(s) 45, 215, 1403
BstF5I GGATG 7 cut(s) 237, 391, 495, 509, 535, 1207, 1291
BstFNI CGCG 1 cut(s) 825
BstHHI GCGC 1 cut(s) 825
BstKTI GATC 7 cut(s) 119, 452, 732, 813, 853, 1060, 1230
BstMAI GTCTC 2 cut(s) 497, 894
BstMBI GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
BstMCI CGRYCG 1 cut(s) 452
BstMWI GCNNNNNNNGC 5 cut(s) 698, 1050, 1251, 1399, 1498
BstNI CCWGG 1 cut(s) 1232
BstNSI RCATGY 3 cut(s) 251, 298, 1427
BstPAI GACNNNNGTC 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 1230, 1333, 1462
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 2 cut(s) 1386, 1439
BstX2I RGATCY 2 cut(s) 850, 1227
BstYI RGATCY 2 cut(s) 850, 1227
BsuRI GGCC 3 cut(s) 390, 677, 1044
BtgZI GCGATG 1 cut(s) 646
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 7 cut(s) 237, 391, 495, 509, 535, 1207, 1291
BtsI GCAGTG 2 cut(s) 638, 1470
BtsIMutI CAGTG 5 cut(s) 638, 936, 1329, 1470, 1503
Cac8I GCNNGC 1 cut(s) 421
CciI TCATGA 2 cut(s) 867, 1535
CfoI GCGC 1 cut(s) 825
Cfr13I GGNCC 3 cut(s) 616, 978, 1042
CseI GACGC 3 cut(s) 715, 933, 984
Csp6I GTAC 5 cut(s) 292, 446, 1124, 1213, 1318
CviQI GTAC 5 cut(s) 292, 446, 1124, 1213, 1318
DdeI CTNAG 3 cut(s) 45, 215, 1403
DpnI GATC 7 cut(s) 118, 451, 731, 812, 852, 1059, 1229
DpnII GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
DraI TTTAAA 2 cut(s) 177, 744
EaeI YGGCCR 1 cut(s) 388
EciI GGCGGA 1 cut(s) 679
Ecl136II GAGCTC 2 cut(s) 49, 430
Eco130I CCWWGG 1 cut(s) 349
Eco24I GRGCYC 3 cut(s) 51, 432, 1247
Eco47I GGWCC 2 cut(s) 616, 978
Eco53kI GAGCTC 2 cut(s) 49, 430
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 425
EcoICRI GAGCTC 2 cut(s) 49, 430
EcoO109I RGGNCCY 2 cut(s) 978, 1042
EcoRII CCWGG 1 cut(s) 1230
EcoT14I CCWWGG 1 cut(s) 349
EcoT38I GRGCYC 3 cut(s) 51, 432, 1247
ErhI CCWWGG 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 497
FalI AAGNNNNNCTT 2 cut(s) 157, 189
FaqI GGGAC 3 cut(s) 991, 1204, 1350
FauNDI CATATG 1 cut(s) 593
FbaI TGATCA 1 cut(s) 1057
Fnu4HI GCNGC 2 cut(s) 1400, 1428
FokI GGATG 7 cut(s) 224, 398, 482, 496, 542, 1194, 1298
FriOI GRGCYC 3 cut(s) 51, 432, 1247
Fsp4HI GCNGC 2 cut(s) 1400, 1428
FspBI CTAG 4 cut(s) 362, 629, 1194, 1518
GlaI GCGC 1 cut(s) 824
GluI GCNGC 2 cut(s) 1400, 1428
GsaI CCCAGC 1 cut(s) 930
HaeIII GGCC 3 cut(s) 390, 677, 1044
HapII CCGG 2 cut(s) 1334, 1464
HgaI GACGC 3 cut(s) 715, 933, 984
HhaI GCGC 1 cut(s) 825
Hin1I GRCGYC 1 cut(s) 707
Hin6I GCGC 1 cut(s) 823
HinP1I GCGC 1 cut(s) 823
HpaII CCGG 2 cut(s) 1334, 1464
HphI GGTGA 5 cut(s) 151, 365, 649, 820, 1516
Hpy166II GTNNAC 1 cut(s) 1148
Hpy188I TCNGA 5 cut(s) 46, 529, 1227, 1249, 1406
Hpy188III TCNNGA 8 cut(s) 406, 476, 571, 733, 861, 868, 1048, 1536
Hpy8I GTNNAC 1 cut(s) 1148
Hpy99I CGWCG 2 cut(s) 290, 794
HpyAV CCTTC 5 cut(s) 79, 346, 676, 1276, 1321
HpyCH4III ACNGT 5 cut(s) 491, 794, 940, 1128, 1387
HpyCH4IV ACGT 4 cut(s) 285, 381, 564, 990
HpyCH4V TGCA 8 cut(s) 55, 132, 206, 559, 597, 1066, 1254, 1427
HpyF10VI GCNNNNNNNGC 5 cut(s) 698, 1050, 1251, 1399, 1498
HpyF3I CTNAG 3 cut(s) 45, 215, 1403
HpySE526I ACGT 4 cut(s) 285, 381, 564, 990
Hsp92I GRCGYC 1 cut(s) 707
HspAI GCGC 1 cut(s) 823
KflI GGGWCCC 1 cut(s) 978
Ksp22I TGATCA 1 cut(s) 1057
Kzo9I GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 2 cut(s) 1386, 1439
MaeI CTAG 4 cut(s) 362, 629, 1194, 1518
MaeII ACGT 4 cut(s) 285, 381, 564, 990
MaeIII GTNAC 6 cut(s) 157, 353, 536, 636, 932, 1198
MalI GATC 7 cut(s) 118, 451, 731, 812, 852, 1059, 1229
MboI GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
MboII GAAGA 7 cut(s) 74, 88, 203, 424, 739, 893, 1288
MflI RGATCY 2 cut(s) 850, 1227
MhlI GDGCHC 3 cut(s) 51, 432, 1247
MlsI TGGCCA 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 3 cut(s) 1026, 1127, 1461
MmeI TCCRAC 3 cut(s) 594, 990, 1205
Mox20I TGGCCA 1 cut(s) 390
MscI TGGCCA 1 cut(s) 390
MseI TTAA 5 cut(s) 176, 743, 1263, 1413, 1481
MslI CAYNNNNRTG 2 cut(s) 246, 1178
Msp20I TGGCCA 1 cut(s) 390
MspCI CTTAAG 1 cut(s) 1480
MspI CCGG 2 cut(s) 1334, 1464
MspR9I CCNGG 3 cut(s) 1232, 1335, 1464
MvaI CCWGG 1 cut(s) 1232
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 5 cut(s) 698, 1050, 1251, 1399, 1498
NciI CCSGG 2 cut(s) 1335, 1464
NdeI CATATG 1 cut(s) 593
NdeII GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
NlaIV GGNNCC 5 cut(s) 404, 618, 979, 980, 1229
NmeAIII GCCGAG 1 cut(s) 718
NmuCI GTSAC 4 cut(s) 157, 353, 636, 932
NspI RCATGY 3 cut(s) 251, 298, 1427
OliI CACNNNNGTG 1 cut(s) 1178
PaeR7I CTCGAG 1 cut(s) 425
PagI TCATGA 2 cut(s) 867, 1535
PciI ACATGT 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PfeI GAWTC 6 cut(s) 221, 773, 864, 887, 1003, 1436
Pfl23II CGTACG 1 cut(s) 1212
PflMI CCANNNNNTGG 1 cut(s) 723
PfoI TCCNGGA 2 cut(s) 1230, 1462
PkrI GCNGC 2 cut(s) 1401, 1429
Ple19I CGATCG 1 cut(s) 452
PleI GAGTC 3 cut(s) 1026, 1127, 1461
PpsI GAGTC 3 cut(s) 1026, 1127, 1461
PpuMI RGGWCCY 1 cut(s) 978
PscI ACATGT 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 155
Psp124BI GAGCTC 2 cut(s) 51, 432
Psp1406I AACGTT 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 1230
PspFI CCCAGC 1 cut(s) 926
PspGI CCWGG 1 cut(s) 1230
PspLI CGTACG 1 cut(s) 1212
PspN4I GGNNCC 5 cut(s) 404, 618, 979, 980, 1229
PspPI GGNCC 3 cut(s) 616, 978, 1042
PspPPI RGGWCCY 1 cut(s) 978
PspXI VCTCGAGB 1 cut(s) 425
PsuI RGATCY 2 cut(s) 850, 1227
PvuI CGATCG 1 cut(s) 452
RsaI GTAC 5 cut(s) 293, 447, 1125, 1214, 1319
RsaNI GTAC 5 cut(s) 292, 446, 1124, 1213, 1318
RseI CAYNNNNRTG 2 cut(s) 246, 1178
SacI GAGCTC 2 cut(s) 51, 432
SaqAI TTAA 5 cut(s) 176, 743, 1263, 1413, 1481
SatI GCNGC 2 cut(s) 1400, 1428
Sau3AI GATC 7 cut(s) 116, 449, 729, 810, 850, 1057, 1227
Sau96I GGNCC 3 cut(s) 616, 978, 1042
SchI GAGTC 3 cut(s) 1026, 1127, 1461
ScrFI CCNGG 3 cut(s) 1232, 1335, 1464
SduI GDGCHC 3 cut(s) 51, 432, 1247
Sfr274I CTCGAG 1 cut(s) 425
SinI GGWCC 2 cut(s) 616, 978
SlaI CTCGAG 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 246, 1178
SmlI CTYRAG 5 cut(s) 163, 425, 895, 1046, 1480
SmoI CTYRAG 5 cut(s) 163, 425, 895, 1046, 1480
SsiI CCGC 4 cut(s) 690, 948, 1089, 1485
SspMI CTAG 4 cut(s) 362, 629, 1194, 1518
SstI GAGCTC 2 cut(s) 51, 432
StyD4I CCNGG 3 cut(s) 1230, 1333, 1462
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 5 cut(s) 491, 794, 940, 1128, 1387
TaiI ACGT 4 cut(s) 288, 384, 567, 993
TatI WGTACW 2 cut(s) 291, 1123
TfiI GAWTC 6 cut(s) 221, 773, 864, 887, 1003, 1436
Tru1I TTAA 5 cut(s) 176, 743, 1263, 1413, 1481
Tru9I TTAA 5 cut(s) 176, 743, 1263, 1413, 1481
TscAI CASTG 5 cut(s) 645, 943, 1329, 1477, 1503
TseFI GTSAC 4 cut(s) 157, 353, 636, 932
TseI GCWGC 2 cut(s) 1399, 1427
Tsp45I GTSAC 4 cut(s) 157, 353, 636, 932
TspDTI ATGAA 4 cut(s) 172, 856, 900, 1394
TspGWI ACGGA 3 cut(s) 502, 555, 1200
TspRI CASTG 5 cut(s) 645, 943, 1329, 1477, 1503
Van91I CCANNNNNTGG 1 cut(s) 723
Vha464I CTTAAG 1 cut(s) 1480
VpaK11BI GGWCC 2 cut(s) 616, 978
XapI RAATTY 6 cut(s) 141, 179, 653, 738, 745, 1259
XceI RCATGY 3 cut(s) 251, 298, 1427
XhoI CTCGAG 1 cut(s) 425
XspI CTAG 4 cut(s) 362, 629, 1194, 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.