Rorug03G0127600

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001140
Physical Location & Seq
Reverse (-)
10311727 .. 10313721
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0127600.1

Sequence Viewer

Length: 243 bp
ATGGCTGAGGGTTTGAGCAATATGTCTATCAAGGAGCTCAAGGGCGTGGAGACCAAACTTGAGAAAGCAATTAGCAGAATTAGATCCAAGAAGGATTGGAAGACATGTTCTCTTAAAGAAAAGCAGAAGGGCAAAGGAGGCATGGTGAAAAAGAAAAGGAGAGAGCTGCTGACCAATTGCATCAAGGTGGGGAAGATGATGAAAGCCTGGGAGAACATCTCATTTCATCTTCTCATACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

80

Amino Acids

9.25

Weight (kDa)

10.26

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 1 - 58 8.6e-09 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 104
AjnI CCWGG 1 cut(s) 206
AloI GAACNNNNNNTCC 2 cut(s) 91, 123
AluBI AGCT 2 cut(s) 37, 166
AluI AGCT 2 cut(s) 37, 166
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 44
AlwI GGATC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 157
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 39
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 1 cut(s) 153
BciT130I CCWGG 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 44
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 203, 235
Bpu10I CCTNAGC 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 23, 80
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 39
BsmAI GTCTC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 1 cut(s) 83
BspPI GGATC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 6
BstKTI GATC 1 cut(s) 86
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 1 cut(s) 83
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
CviAII CATG 2 cut(s) 105, 142
CviJI RGCY 4 cut(s) 5, 37, 166, 206
CviKI_1 RGCY 4 cut(s) 5, 37, 166, 206
DdeI CTNAG 1 cut(s) 6
DpnI GATC 1 cut(s) 85
DpnII GATC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 206
EcoT38I GRGCYC 1 cut(s) 39
FaeI CATG 2 cut(s) 108, 145
FaiI YATR 4 cut(s) 23, 106, 143, 236
FatI CATG 2 cut(s) 104, 141
Fnu4HI GCNGC 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 1 cut(s) 167
GluI GCNGC 1 cut(s) 167
Hin1II CATG 2 cut(s) 108, 145
HphI GGTGA 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 85, 121
HpyCH4V TGCA 1 cut(s) 180
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 2 cut(s) 108, 145
Kzo9I GATC 1 cut(s) 83
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 2 cut(s) 193, 220
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 1 cut(s) 189
MalI GATC 1 cut(s) 85
MboI GATC 1 cut(s) 83
MboII GAAGA 3 cut(s) 112, 205, 221
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 83
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 3 cut(s) 69, 78, 175
MnlI CCTC 1 cut(s) 131
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 208
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 138
NdeII GATC 1 cut(s) 83
NlaIII CATG 2 cut(s) 108, 145
NspI RCATGY 1 cut(s) 108
PciI ACATGT 1 cut(s) 104
PkrI GCNGC 1 cut(s) 168
PscI ACATGT 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 208
SduI GDGCHC 1 cut(s) 39
SetI ASST 3 cut(s) 39, 168, 189
SfaNI GCATC 1 cut(s) 189
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 38, 59
SmoI CTYRAG 2 cut(s) 38, 59
Sse9I AATT 3 cut(s) 69, 78, 175
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 206
TasI AATT 3 cut(s) 69, 78, 175
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TseI GCWGC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 215, 215
XceI RCATGY 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.