Rh3DG169500

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001140
Physical Location & Seq
Forward (+)
14820031 .. 14826255
6225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG169500.1

Sequence Viewer

Length: 975 bp
ATGGAATTAGCTCAGACCCTCTTCCTCCCTCTCGTTCTGTGTCTTCTCTCTCTTTTAACACAACAAAGTGCGCTACTGGTCTCAGCTGGTCACCACCATGGTCACCATGGCCATCACCACCATGGTCACCATGGCCATCACCACCATCATACACACCGATACTTGTTCGATTGGCGTCCAACGAAACTGTTTGTTTTCGGAGACTCGTACTCCGACACAGGCAACAACATGAAGTCTGTGACCAAATCTTGGAAATATCCTTACGGAATCACCTTCCCGGGAAAACCCACCGGCCGTTTCTCCGATGGCCGCGTCCTCACTGATTTCCTTGCTAAGTTTGTAGGGGTGAAGTCTCCAATACCATACAAATTTAGAAATGTCGGAGTTAAACATTTGAAATACGGAATGAATTTCGCATATGGAGGCACCGGTGTGTTCAATACCTTGGTTGCGGCACCAAACATGACCACCCAAATCGATTTTTTTCAGCAATTCATCAAAGACTCCGTTTTCAATGCCCAAGACCTACACTCCTCCGTGGCATTGATCACTCTTGCTGGCAATGACTACGCATATTACCTGGCCCTGAATAATTCTTCTGAGGGTTTTAAACCTTTCATAACATCAGTTGTAAATCAACTAAGTGTGAACCTGAAACGTGTCTATGACTTGGGAGTGAAGAAAATAGTTGTGACAGCTCTACAACCTTTGGGATGTCTTCCTGGTAGCACAGCCGAATTATCATTCCAGAAGTGCAATGGAACACAAAACTTGCTGGTCGGTTTCCACAACCTCTTGTTGCAGCAAGCTATTGCCAAGTTGAACAATGAATCCAAGAGTTCTTTCATCGTTCTCGATCTATATACCTCATTCATGTCCGTGCTGAAGAATAAAGGAGACCATCTAGGTAACTATCTCTTACCAAAAAAAAAAAGAATTACATGGTTAACATTATTGCTCTTTTTAGTATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

324

Amino Acids

36.29

Weight (kDa)

9.62

Isoelectric Point (pI)

21.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 63 - 290 8.9e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 973
AccB1I GGYRCC 2 cut(s) 425, 454
AccB7I CCANNNNNTGG 1 cut(s) 249
AccII CGCG 1 cut(s) 312
AciI CCGC 2 cut(s) 310, 452
AcoI YGGCCR 4 cut(s) 109, 133, 292, 307
AcsI RAATTY 2 cut(s) 368, 409
AcuI CTGAAG 1 cut(s) 905
AcyI GRCGYC 1 cut(s) 175
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 249
AflIII ACRYGT 1 cut(s) 658
AgeI ACCGGT 1 cut(s) 428
AgsI TTSAA 4 cut(s) 397, 439, 514, 823
AjnI CCWGG 2 cut(s) 579, 721
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
AleI CACNNNNGTG 1 cut(s) 431
AloI GAACNNNNNNTCC 2 cut(s) 815, 847
AluBI AGCT 4 cut(s) 11, 86, 698, 809
AluI AGCT 4 cut(s) 11, 86, 698, 809
Alw26I GTCTC 4 cut(s) 85, 195, 357, 891
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 5 cut(s) 109, 133, 292, 307, 582
ApeKI GCWGC 1 cut(s) 802
ApoI RAATTY 2 cut(s) 368, 409
AsiGI ACCGGT 1 cut(s) 428
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 583
AsuC2I CCSGG 2 cut(s) 278, 279
AsuHPI GGTGA 7 cut(s) 83, 95, 107, 119, 131, 262, 358
AvaI CYCGRG 1 cut(s) 277
BalI TGGCCA 2 cut(s) 111, 135
BanI GGYRCC 2 cut(s) 425, 454
BbsI GAAGAC 2 cut(s) 35, 710
BbvI GCAGC 1 cut(s) 814
BccI CCATC 5 cut(s) 120, 144, 153, 299, 909
BceAI ACGGC 1 cut(s) 279
BciT130I CCWGG 2 cut(s) 581, 723
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 4 cut(s) 85, 195, 357, 891
BfaI CTAG 1 cut(s) 905
BisI GCNGC 3 cut(s) 310, 453, 803
BlsI GCNGC 3 cut(s) 311, 454, 804
Bme1390I CCNGG 4 cut(s) 278, 279, 581, 723
BmeT110I CYCGRG 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 583
BmiI GGNNCC 2 cut(s) 427, 456
BmrFI CCNGG 4 cut(s) 278, 279, 581, 723
BpiI GAAGAC 2 cut(s) 35, 710
BpuMI CCSGG 2 cut(s) 278, 279
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 175
BsaI GGTCTC 2 cut(s) 85, 891
BsaJI CCNNGG 7 cut(s) 97, 106, 121, 130, 277, 444, 537
BsaWI WCCGGW 1 cut(s) 428
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 515, 545
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse118I RCCGGY 2 cut(s) 290, 428
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 2 cut(s) 568, 763
BseBI CCWGG 2 cut(s) 581, 723
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 7 cut(s) 97, 106, 121, 130, 277, 444, 537
BseGI GGATG 1 cut(s) 719
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 2 cut(s) 568, 763
BseMII CTCAG 3 cut(s) 26, 96, 591
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 523
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 1 cut(s) 814
Bsh1236I CGCG 1 cut(s) 312
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 5 cut(s) 111, 135, 294, 309, 584
BshNI GGYRCC 2 cut(s) 425, 454
BshTI ACCGGT 1 cut(s) 428
BshVI ATCGAT 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 295
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 3 cut(s) 278, 291, 429
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 4 cut(s) 85, 195, 357, 891
BsnI GGCC 5 cut(s) 111, 135, 294, 309, 584
Bso31I GGTCTC 2 cut(s) 85, 891
BsoBI CYCGRG 1 cut(s) 277
Bsp143I GATC 2 cut(s) 546, 856
Bsp19I CCATGG 4 cut(s) 97, 106, 121, 130
BspACI CCGC 2 cut(s) 310, 452
BspANI GGCC 5 cut(s) 111, 135, 294, 309, 584
BspCNI CTCAG 3 cut(s) 25, 95, 592
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 427, 456
BspT107I GGYRCC 2 cut(s) 425, 454
BspTNI GGTCTC 2 cut(s) 85, 891
BsrDI GCAATG 2 cut(s) 568, 763
BsrFI RCCGGY 2 cut(s) 290, 428
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 290, 428
BssECI CCNNGG 7 cut(s) 97, 106, 121, 130, 277, 444, 537
BssMI GATC 2 cut(s) 546, 856
BssNI GRCGYC 1 cut(s) 175
BssT1I CCWWGG 5 cut(s) 97, 106, 121, 130, 444
Bst2UI CCWGG 2 cut(s) 581, 723
Bst4CI ACNGT 1 cut(s) 189
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 175
BstC8I GCNNGC 2 cut(s) 559, 807
BstDEI CTNAG 5 cut(s) 12, 82, 333, 600, 641
BstDSI CCRYGG 5 cut(s) 97, 106, 121, 130, 537
BstEII GGTNACC 3 cut(s) 89, 101, 125
BstF5I GGATG 1 cut(s) 719
BstFNI CGCG 1 cut(s) 312
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 549, 859
BstMAI GTCTC 4 cut(s) 85, 195, 357, 891
BstMBI GATC 2 cut(s) 546, 856
BstMCI CGRYCG 1 cut(s) 295
BstNI CCWGG 2 cut(s) 581, 723
BstPI GGTNACC 3 cut(s) 89, 101, 125
BstSCI CCNGG 4 cut(s) 276, 277, 579, 721
BstUI CGCG 1 cut(s) 312
BstV1I GCAGC 1 cut(s) 814
BstV2I GAAGAC 2 cut(s) 35, 710
BstZI CGGCCG 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 5 cut(s) 111, 135, 294, 309, 584
BsuTUI ATCGAT 1 cut(s) 477
BtgI CCRYGG 5 cut(s) 97, 106, 121, 130, 537
BtsCI GGATG 1 cut(s) 719
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 559, 807
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 2 cut(s) 290, 428
Cfr13I GGNCC 1 cut(s) 583
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 2 cut(s) 164, 301
Csp6I GTAC 1 cut(s) 208
CspAI ACCGGT 1 cut(s) 428
CviAII CATG 8 cut(s) 98, 107, 122, 131, 229, 463, 874, 942
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 5 cut(s) 12, 82, 333, 600, 641
DpnI GATC 2 cut(s) 548, 858
DpnII GATC 2 cut(s) 546, 856
DraI TTTAAA 1 cut(s) 610
EaeI YGGCCR 4 cut(s) 109, 133, 292, 307
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 292
Eco130I CCWWGG 5 cut(s) 97, 106, 121, 130, 444
Eco31I GGTCTC 2 cut(s) 85, 891
Eco52I CGGCCG 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 277
Eco91I GGTNACC 3 cut(s) 89, 101, 125
EcoO65I GGTNACC 3 cut(s) 89, 101, 125
EcoRII CCWGG 2 cut(s) 579, 721
EcoT14I CCWWGG 5 cut(s) 97, 106, 121, 130, 444
ErhI CCWWGG 5 cut(s) 97, 106, 121, 130, 444
FaeI CATG 8 cut(s) 101, 110, 125, 134, 232, 466, 877, 945
FatI CATG 8 cut(s) 97, 106, 121, 130, 228, 462, 873, 941
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 546
Fnu4HI GCNGC 3 cut(s) 310, 453, 803
FokI GGATG 1 cut(s) 726
Fsp4HI GCNGC 3 cut(s) 310, 453, 803
FspBI CTAG 1 cut(s) 905
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 310, 453, 803
HaeIII GGCC 5 cut(s) 111, 135, 294, 309, 584
HapII CCGG 3 cut(s) 278, 291, 429
HgaI GACGC 2 cut(s) 164, 301
HhaI GCGC 1 cut(s) 73
Hin1I GRCGYC 1 cut(s) 175
Hin1II CATG 8 cut(s) 101, 110, 125, 134, 232, 466, 877, 945
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HinfI GANTC 4 cut(s) 203, 267, 503, 830
HpaI GTTAAC 1 cut(s) 948
HpaII CCGG 3 cut(s) 278, 291, 429
HphI GGTGA 7 cut(s) 83, 95, 107, 119, 131, 262, 358
Hpy166II GTNNAC 2 cut(s) 649, 948
Hpy188I TCNGA 6 cut(s) 15, 200, 214, 304, 383, 601
Hpy188III TCNNGA 2 cut(s) 748, 854
Hpy8I GTNNAC 2 cut(s) 649, 948
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 2 cut(s) 756, 802
HpyF3I CTNAG 5 cut(s) 12, 82, 333, 600, 641
HpySE526I ACGT 1 cut(s) 658
Hsp92I GRCGYC 1 cut(s) 175
Hsp92II CATG 8 cut(s) 101, 110, 125, 134, 232, 466, 877, 945
HspAI GCGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 546
KspAI GTTAAC 1 cut(s) 948
Kzo9I GATC 2 cut(s) 546, 856
Lsp1109I GCAGC 1 cut(s) 814
MaeI CTAG 1 cut(s) 905
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 6 cut(s) 89, 101, 125, 238, 691, 908
MalI GATC 2 cut(s) 548, 858
MboI GATC 2 cut(s) 546, 856
MboII GAAGA 6 cut(s) 13, 35, 588, 691, 710, 898
MlsI TGGCCA 2 cut(s) 111, 135
MluCI AATT 7 cut(s) 5, 368, 409, 491, 592, 737, 936
MluNI TGGCCA 2 cut(s) 111, 135
MlyI GAGTC 2 cut(s) 197, 497
MmeI TCCRAC 3 cut(s) 203, 237, 361
MnlI CCTC 9 cut(s) 29, 35, 39, 326, 416, 544, 595, 803, 877
Mox20I TGGCCA 2 cut(s) 111, 135
MscI TGGCCA 2 cut(s) 111, 135
MseI TTAA 4 cut(s) 56, 387, 609, 947
MslI CAYNNNNRTG 4 cut(s) 96, 120, 431, 878
Msp20I TGGCCA 2 cut(s) 111, 135
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 3 cut(s) 278, 291, 429
MspR9I CCNGG 4 cut(s) 278, 279, 581, 723
MvaI CCWGG 2 cut(s) 581, 723
MvnI CGCG 1 cut(s) 312
NciI CCSGG 2 cut(s) 278, 279
NcoI CCATGG 4 cut(s) 97, 106, 121, 130
NdeI CATATG 1 cut(s) 418
NdeII GATC 2 cut(s) 546, 856
NlaIII CATG 8 cut(s) 101, 110, 125, 134, 232, 466, 877, 945
NlaIV GGNNCC 2 cut(s) 427, 456
NmuCI GTSAC 5 cut(s) 89, 101, 125, 238, 691
OliI CACNNNNGTG 1 cut(s) 431
PfeI GAWTC 2 cut(s) 267, 830
PflMI CCANNNNNTGG 1 cut(s) 249
PinAI ACCGGT 1 cut(s) 428
PkrI GCNGC 3 cut(s) 311, 454, 804
PleI GAGTC 2 cut(s) 197, 497
PpsI GAGTC 2 cut(s) 197, 497
PsiI TTATAA 1 cut(s) 973
Psp6I CCWGG 2 cut(s) 579, 721
PspEI GGTNACC 3 cut(s) 89, 101, 125
PspGI CCWGG 2 cut(s) 579, 721
PspN4I GGNNCC 2 cut(s) 427, 456
PspPI GGNCC 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 4 cut(s) 96, 120, 431, 878
SaqAI TTAA 4 cut(s) 56, 387, 609, 947
SatI GCNGC 3 cut(s) 310, 453, 803
Sau3AI GATC 2 cut(s) 546, 856
Sau96I GGNCC 1 cut(s) 583
SchI GAGTC 2 cut(s) 197, 497
ScrFI CCNGG 4 cut(s) 278, 279, 581, 723
SgrAI CRCCGGYG 1 cut(s) 428
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 4 cut(s) 96, 120, 431, 878
Sse9I AATT 7 cut(s) 5, 368, 409, 491, 592, 737, 936
SsiI CCGC 2 cut(s) 310, 452
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 4 cut(s) 276, 277, 579, 721
StyI CCWWGG 5 cut(s) 97, 106, 121, 130, 444
TaaI ACNGT 1 cut(s) 189
TaiI ACGT 1 cut(s) 661
TaqI TCGA 3 cut(s) 168, 477, 855
TasI AATT 7 cut(s) 5, 368, 409, 491, 592, 737, 936
TauI GCSGC 2 cut(s) 312, 455
TfiI GAWTC 2 cut(s) 267, 830
Tru1I TTAA 4 cut(s) 56, 387, 609, 947
Tru9I TTAA 4 cut(s) 56, 387, 609, 947
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 5 cut(s) 89, 101, 125, 238, 691
TseI GCWGC 1 cut(s) 802
Tsp45I GTSAC 5 cut(s) 89, 101, 125, 238, 691
TspDTI ATGAA 7 cut(s) 245, 422, 484, 607, 835, 843, 862
TspGWI ACGGA 5 cut(s) 279, 417, 496, 526, 868
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 249
XapI RAATTY 2 cut(s) 368, 409
XcmI CCANNNNNNNNNTGG 4 cut(s) 104, 119, 128, 755
XmaI CCCGGG 1 cut(s) 277
XspI CTAG 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.