pycom07g05060

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001140
Physical Location & Seq
Forward (+)
4332026 .. 4333047
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05060.3

Sequence Viewer

Length: 621 bp
ATGTATATATCGTCCTCATCCCAGCATCTTTCCCCCAGAACATTACTCCTAATATTCTGGTTTCAATGGAGAAGAAAACCACCGTCGCCTCTTCATTTTGTCTCCTTCTTTTATGTGTCGCCACTTCTACTGGCACACCCCCACCTTCACCACCAACCTCACAAAAATGATGGTTCCATCAAACTTTTTGTATTCGGGGACTCGTATGCTGATACCGGGAATGTTGGAAAATCTGTATCTCCTTCGTGGAAGGAACCTTATGGGATCACTTTTCCTGGAAAGCCCGCCGGCCGCTTTTCAGATGGTCGTGTGCTCACTGATTATTTGGCTCAATTTTTGGGTATAAGATCTCCATTACCTTACGCATTGAGAAAATATGCGAAGAAATCGAAACTGGAGAACGGGATGAACTTTGCATATGGAGGGACGGGAGTCTTCGATACGTTGGTGAGTGGACCAAACTTGACCACCCAAATCGGCTTTTTCCAACATCTGCTTGAACAAGAAAAAGTGTATGACAAACGCGACCTAATTAAATCCTCCGTAGCTCTAGTGTCAGTTGCGGGGAACGACTACACAACTCATTTCGTTAAACAAGGCAATGGCAGTAAGTCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

207

Amino Acids

23.07

Weight (kDa)

9.75

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 525
AciI CCGC 3 cut(s) 285, 292, 563
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 289
AgsI TTSAA 2 cut(s) 65, 500
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 1 cut(s) 548
AluI AGCT 1 cut(s) 548
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 272
AoxI GGCC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 2 cut(s) 140, 460
AvaII GGWCC 1 cut(s) 455
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BbsI GAAGAC 2 cut(s) 427, 606
Bbv12I GWGCWC 1 cut(s) 315
BccI CCATC 3 cut(s) 164, 185, 296
BciT130I CCWGG 1 cut(s) 276
BcnI CCSGG 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 551
BglII AGATCT 1 cut(s) 347
BisI GCNGC 1 cut(s) 292
BlsI GCNGC 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 217, 276
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 175, 255
BmrFI CCNGG 2 cut(s) 217, 276
BmsI GCATC 1 cut(s) 34
BoxI GACNNNNGTC 1 cut(s) 431
BpiI GAAGAC 2 cut(s) 427, 606
BpmI CTGGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 135, 399
Bse3DI GCAATG 1 cut(s) 607
BseBI CCWGG 1 cut(s) 276
BseGI GGATG 2 cut(s) 17, 411
BseMI GCAATG 1 cut(s) 607
BseNI ACTGG 2 cut(s) 135, 399
BseX3I CGGCCG 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 21
Bsh1236I CGCG 1 cut(s) 525
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 2 cut(s) 216, 288
BslFI GGGAC 2 cut(s) 212, 439
BsmAI GTCTC 1 cut(s) 106
BsmFI GGGAC 2 cut(s) 212, 439
BsnI GGCC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 264, 347
BspACI CCGC 3 cut(s) 285, 292, 563
BspANI GGCC 1 cut(s) 291
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 2 cut(s) 175, 255
BspPI GGATC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 607
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 135, 399
BssAI RCCGGY 1 cut(s) 287
BssMI GATC 2 cut(s) 264, 347
Bst2UI CCWGG 1 cut(s) 276
Bst4CI ACNGT 1 cut(s) 84
Bst6I CTCTTC 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 285, 289
BstF5I GGATG 2 cut(s) 17, 411
BstFNI CGCG 1 cut(s) 525
BstKTI GATC 2 cut(s) 267, 350
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 264, 347
BstMCI CGRYCG 1 cut(s) 292
BstNI CCWGG 1 cut(s) 276
BstPAI GACNNNNGTC 1 cut(s) 431
BstSCI CCNGG 2 cut(s) 215, 274
BstUI CGCG 1 cut(s) 525
BstV2I GAAGAC 2 cut(s) 427, 606
BstX2I RGATCY 1 cut(s) 347
BstYI RGATCY 1 cut(s) 347
BstZI CGGCCG 1 cut(s) 289
BsuRI GGCC 1 cut(s) 291
BtsCI GGATG 2 cut(s) 17, 411
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 2 cut(s) 285, 289
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 455
CviJI RGCY 5 cut(s) 283, 291, 329, 480, 548
CviKI_1 RGCY 5 cut(s) 283, 291, 329, 480, 548
DpnI GATC 2 cut(s) 266, 349
DpnII GATC 2 cut(s) 264, 347
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EclXI CGGCCG 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 455
Eco52I CGGCCG 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 274
FaqI GGGAC 2 cut(s) 212, 439
FauI CCCGC 2 cut(s) 292, 556
FauNDI CATATG 1 cut(s) 418
Fnu4HI GCNGC 1 cut(s) 292
FokI GGATG 2 cut(s) 4, 418
Fsp4HI GCNGC 1 cut(s) 292
FspBI CTAG 1 cut(s) 551
GluI GCNGC 1 cut(s) 292
GsaI CCCAGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 1 cut(s) 291
HapII CCGG 2 cut(s) 216, 288
HinfI GANTC 2 cut(s) 200, 432
HpaII CCGG 2 cut(s) 216, 288
HphI GGTGA 2 cut(s) 140, 460
Hpy166II GTNNAC 1 cut(s) 455
Hpy188I TCNGA 1 cut(s) 301
Hpy188III TCNNGA 1 cut(s) 618
Hpy8I GTNNAC 1 cut(s) 455
Hpy99I CGWCG 1 cut(s) 88
HpyAV CCTTC 4 cut(s) 115, 155, 244, 252
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 1 cut(s) 416
HpySE526I ACGT 1 cut(s) 443
KroI GCCGGC 1 cut(s) 287
KroNI GCCGGC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 264, 347
LpnPI CCDG 9 cut(s) 35, 43, 49, 116, 229, 261, 288, 301, 380
LweI GCATC 1 cut(s) 34
MaeI CTAG 1 cut(s) 551
MaeII ACGT 1 cut(s) 443
MalI GATC 2 cut(s) 266, 349
MboI GATC 2 cut(s) 264, 347
MboII GAAGA 5 cut(s) 83, 84, 394, 427, 606
MflI RGATCY 1 cut(s) 347
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 2 cut(s) 332, 531
MlyI GAGTC 2 cut(s) 194, 441
MmeI TCCRAC 2 cut(s) 205, 511
MnlI CCTC 5 cut(s) 25, 99, 168, 416, 550
MroNI GCCGGC 1 cut(s) 287
MseI TTAA 2 cut(s) 534, 591
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 2 cut(s) 216, 288
MspR9I CCNGG 2 cut(s) 217, 276
MvaI CCWGG 1 cut(s) 276
MvnI CGCG 1 cut(s) 525
NaeI GCCGGC 1 cut(s) 289
NciI CCSGG 1 cut(s) 217
NdeI CATATG 1 cut(s) 418
NdeII GATC 2 cut(s) 264, 347
NgoMIV GCCGGC 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 175, 255
PdiI GCCGGC 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 1 cut(s) 293
PleI GAGTC 2 cut(s) 194, 440
PpsI GAGTC 2 cut(s) 194, 440
PshAI GACNNNNGTC 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 21
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 2 cut(s) 175, 255
PspPI GGNCC 1 cut(s) 455
PsuI RGATCY 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 2 cut(s) 534, 591
SatI GCNGC 1 cut(s) 292
Sau3AI GATC 2 cut(s) 264, 347
Sau96I GGNCC 1 cut(s) 455
SchI GAGTC 2 cut(s) 194, 441
ScrFI CCNGG 2 cut(s) 217, 276
SduI GDGCHC 1 cut(s) 315
SetI ASST 7 cut(s) 147, 160, 259, 361, 446, 531, 550
SfaNI GCATC 1 cut(s) 34
SinI GGWCC 1 cut(s) 455
SmiMI CAYNNNNRTG 1 cut(s) 165
Sse9I AATT 2 cut(s) 332, 531
SsiI CCGC 3 cut(s) 285, 292, 563
SspI AATATT 1 cut(s) 54
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 215, 274
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 446
TaqI TCGA 2 cut(s) 389, 438
TasI AATT 2 cut(s) 332, 531
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 534, 591
Tru9I TTAA 2 cut(s) 534, 591
TscAI CASTG 1 cut(s) 322
TspDTI ATGAA 2 cut(s) 83, 422
TspGWI ACGGA 1 cut(s) 532
TspRI CASTG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 455
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.