Prupe.2G080100_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001140
Physical Location & Seq
Forward (+)
12236341 .. 12239078
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G080100.1

Sequence Viewer

Length: 1071 bp
ATGGAGAAGCAAGCAGCCATAGCCACTTCTTGTCTCTTCCTTTTATACATCGCCACGTTTACAGTGGCAGAAGTTCTTGAATCACATCATCACCATCATCGTCATAACCATCATGGTTCTGTGAAGCTTTTCGTTTTTGGGGACTCGTATGCTGATACTGGAAATACCGAAAAATCTGTGTCTGCTTCATGGAAGGAACCTTATGGCATCAATTTTCCGGGAAAGCCAGCTGGTCGATTTTCAGATGGTCTCGTGCTTACTGATTATATAGCTTCCTTTTTGGGCATAAGATCTCCAGTGCCTTACACATTGAGAAAATTTGTGAAGAAATCAAAACTAGAGTCTGGGATGAACTTTGCATATGGAGGGACAGGCGTTTTTGATACAGTGTTTGGTGGACCAAACTTGACCACCCAAATCGACTTTTTCCAACAGCTGCTTGAACAAAAACTCTATAGTACCAAAAATGACGTCGTTGACTCGTCCATTGCTCTCGTATCAGTTGCGGGTAACGACTACGCTGCTTATTTTGGCAACCACACTGAGGATTTCGCAGCGGCCACCAAATCCATTATTAAGCAGCTTGCTGTGGATCTAGAGCGTATATATGGGTTGGGAGTGGGAAAAATAGCTGTTACAGCAATAGGGCCCCTGGGATGTCTGCCGCGCATGACTTCCTTTCTTTCATATCAGAATTGTAGTGAAGTTGCCAATCTGGTTTCAATTTTCCATAACCAGATTTTGCGTCAAAAAGTTGAAGAGTTGAACAAGGAGACTAAGAAATCTTCATTTTTAATCTTGGATCTTTATAACGCGTCTTTGTCTGCAATTATGCTCCCAAAACACCATCAAGGATATTCGACACTCCAGATTAATCCACTAAAGCCGTGCTGTGTTGGGGTGAGCAATGAATACTCATGCGGGAGTGTGAATGAGAGTGGAGCAAAGAAGTACACTGTATGCGATAAGCCCAAACTTTCTTTCTTTTGGGACACAATTCACCCTTCGCAGAATGGCTGGCATGAAGTTTATTCCGCTATAAAATCTTCTCTTCGTCAATTGTATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

39.38

Weight (kDa)

8.33

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AatII GACGTC 1 cut(s) 474
AccII CGCG 2 cut(s) 667, 815
AciI CCGC 5 cut(s) 506, 557, 665, 921, 1035
AclWI GGATC 2 cut(s) 600, 810
AcoI YGGCCR 1 cut(s) 558
AcsI RAATTY 1 cut(s) 317
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 2 cut(s) 460, 953
AfiI CCNNNNNNNGG 1 cut(s) 544
AflIII ACRYGT 1 cut(s) 813
AgsI TTSAA 5 cut(s) 80, 443, 723, 758, 766
AjnI CCWGG 1 cut(s) 651
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AluBI AGCT 6 cut(s) 127, 230, 272, 436, 583, 632
AluI AGCT 6 cut(s) 127, 230, 272, 436, 583, 632
Alw26I GTCTC 3 cut(s) 38, 254, 767
AlwI GGATC 2 cut(s) 600, 810
AoxI GGCC 2 cut(s) 558, 647
ApaI GGGCCC 1 cut(s) 651
ApeKI GCWGC 5 cut(s) 14, 436, 521, 554, 580
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 4 cut(s) 326, 358, 456, 488
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 669
AspS9I GGNCC 3 cut(s) 398, 647, 648
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 83, 913, 992
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 651
BanII GRGCYC 1 cut(s) 651
BauI CACGAG 1 cut(s) 251
BbvI GCAGC 5 cut(s) 26, 423, 508, 566, 592
BccI CCATC 4 cut(s) 102, 117, 239, 855
BceAI ACGGC 1 cut(s) 871
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 1 cut(s) 653
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 3 cut(s) 38, 254, 767
BfaI CTAG 2 cut(s) 338, 596
BfmI CTRYAG 1 cut(s) 454
BglII AGATCT 1 cut(s) 290
BisI GCNGC 7 cut(s) 15, 437, 522, 555, 558, 581, 665
BlsI GCNGC 7 cut(s) 16, 438, 523, 556, 559, 582, 666
Bme1390I CCNGG 2 cut(s) 219, 653
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 398, 647, 648
BmiI GGNNCC 3 cut(s) 198, 649, 650
BmrFI CCNGG 2 cut(s) 219, 653
BmsI GCATC 1 cut(s) 216
BpmI CTGGAG 2 cut(s) 279, 851
BpuMI CCSGG 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 651, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse1I ACTGG 2 cut(s) 163, 296
Bse3DI GCAATG 2 cut(s) 486, 913
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 2 cut(s) 651, 652
BseGI GGATG 2 cut(s) 354, 662
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMI GCAATG 2 cut(s) 486, 913
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 2 cut(s) 163, 296
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 5 cut(s) 26, 423, 508, 566, 592
Bsh1236I CGCG 2 cut(s) 667, 815
BshFI GGCC 2 cut(s) 560, 649
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 3 cut(s) 155, 382, 1004
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 3 cut(s) 38, 254, 767
BsmFI GGGAC 3 cut(s) 155, 382, 1004
BsnI GGCC 2 cut(s) 560, 649
Bso31I GGTCTC 1 cut(s) 254
Bsp120I GGGCCC 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 3 cut(s) 290, 592, 802
BspACI CCGC 5 cut(s) 506, 557, 665, 921, 1035
BspANI GGCC 2 cut(s) 560, 649
BspCNI CTCAG 1 cut(s) 535
BspFNI CGCG 2 cut(s) 667, 815
BspLI GGNNCC 3 cut(s) 198, 649, 650
BspPI GGATC 2 cut(s) 600, 810
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 2 cut(s) 486, 913
BsrI ACTGG 2 cut(s) 163, 296
BssECI CCNNGG 2 cut(s) 651, 652
BssMI GATC 3 cut(s) 290, 592, 802
BssNI GRCGYC 1 cut(s) 471
BssSI CACGAG 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 653
Bst4CI ACNGT 3 cut(s) 64, 388, 958
Bst6I CTCTTC 3 cut(s) 41, 753, 1056
BstACI GRCGYC 1 cut(s) 471
BstC8I GCNNGC 4 cut(s) 12, 228, 585, 1019
BstDEI CTNAG 2 cut(s) 543, 777
BstF5I GGATG 2 cut(s) 354, 662
BstFNI CGCG 2 cut(s) 667, 815
BstHHI GCGC 1 cut(s) 669
BstKTI GATC 3 cut(s) 293, 595, 805
BstMAI GTCTC 3 cut(s) 38, 254, 767
BstMBI GATC 3 cut(s) 290, 592, 802
BstMWI GCNNNNNNNGC 2 cut(s) 20, 638
BstNI CCWGG 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 217, 651
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 651
BstUI CGCG 2 cut(s) 667, 815
BstV1I GCAGC 5 cut(s) 26, 423, 508, 566, 592
BstX2I RGATCY 3 cut(s) 290, 592, 802
BstYI RGATCY 3 cut(s) 290, 592, 802
BsuRI GGCC 2 cut(s) 560, 649
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 354, 662
BtsIMutI CAGTG 5 cut(s) 69, 303, 393, 540, 954
Cac8I GCNNGC 4 cut(s) 12, 228, 585, 1019
CfoI GCGC 1 cut(s) 669
Cfr13I GGNCC 3 cut(s) 398, 647, 648
CseI GACGC 2 cut(s) 734, 804
Csp6I GTAC 2 cut(s) 459, 952
CviAII CATG 5 cut(s) 113, 189, 670, 918, 1022
CviQI GTAC 2 cut(s) 459, 952
DdeI CTNAG 2 cut(s) 543, 777
DpnI GATC 3 cut(s) 292, 594, 804
DpnII GATC 3 cut(s) 290, 592, 802
EaeI YGGCCR 1 cut(s) 558
Eam1104I CTCTTC 3 cut(s) 41, 753, 1056
EarI CTCTTC 3 cut(s) 41, 753, 1056
Eco24I GRGCYC 1 cut(s) 651
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 398
EcoO109I RGGNCCY 2 cut(s) 647, 648
EcoRII CCWGG 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 651
FaeI CATG 5 cut(s) 116, 192, 673, 921, 1025
FaqI GGGAC 3 cut(s) 155, 382, 1004
FatI CATG 5 cut(s) 112, 188, 669, 917, 1021
FauI CCCGC 2 cut(s) 499, 914
FauNDI CATATG 1 cut(s) 361
Fnu4HI GCNGC 7 cut(s) 15, 437, 522, 555, 558, 581, 665
FokI GGATG 2 cut(s) 361, 669
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 7 cut(s) 15, 437, 522, 555, 558, 581, 665
FspBI CTAG 2 cut(s) 338, 596
GlaI GCGC 1 cut(s) 668
GluI GCNGC 7 cut(s) 15, 437, 522, 555, 558, 581, 665
GsuI CTGGAG 2 cut(s) 279, 851
HaeIII GGCC 2 cut(s) 560, 649
HapII CCGG 1 cut(s) 218
HgaI GACGC 2 cut(s) 734, 804
HhaI GCGC 1 cut(s) 669
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 5 cut(s) 116, 192, 673, 921, 1025
Hin6I GCGC 1 cut(s) 667
HinP1I GCGC 1 cut(s) 667
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 80, 143, 341, 479
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 83, 913, 992
Hpy166II GTNNAC 4 cut(s) 60, 398, 478, 954
Hpy188I TCNGA 2 cut(s) 244, 693
Hpy188III TCNNGA 3 cut(s) 77, 596, 868
Hpy8I GTNNAC 4 cut(s) 60, 398, 478, 954
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 2 cut(s) 187, 1014
HpyCH4III ACNGT 3 cut(s) 64, 388, 958
HpyCH4IV ACGT 2 cut(s) 56, 471
HpyCH4V TGCA 2 cut(s) 359, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 638
HpyF3I CTNAG 2 cut(s) 543, 777
HpySE526I ACGT 2 cut(s) 56, 471
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 5 cut(s) 116, 192, 673, 921, 1025
HspAI GCGC 1 cut(s) 667
Kzo9I GATC 3 cut(s) 290, 592, 802
LmnI GCTCC 2 cut(s) 840, 941
Lsp1109I GCAGC 5 cut(s) 26, 423, 508, 566, 592
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 338, 596
MaeII ACGT 2 cut(s) 56, 471
MaeIII GTNAC 2 cut(s) 509, 634
MalI GATC 3 cut(s) 292, 594, 804
MboI GATC 3 cut(s) 290, 592, 802
MboII GAAGA 6 cut(s) 28, 337, 770, 777, 1038, 1043
MfeI CAATTG 1 cut(s) 1058
MflI RGATCY 3 cut(s) 290, 592, 802
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 7 cut(s) 211, 317, 694, 723, 828, 996, 1058
MluI ACGCGT 1 cut(s) 813
MlyI GAGTC 3 cut(s) 137, 350, 473
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 2 cut(s) 359, 538
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 3 cut(s) 576, 794, 873
MspA1I CMGCKG 3 cut(s) 230, 436, 557
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 219, 653
MunI CAATTG 1 cut(s) 1058
MvaI CCWGG 1 cut(s) 653
MvnI CGCG 2 cut(s) 667, 815
MwoI GCNNNNNNNGC 2 cut(s) 20, 638
NciI CCSGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 361
NdeII GATC 3 cut(s) 290, 592, 802
NlaIII CATG 5 cut(s) 116, 192, 673, 921, 1025
NlaIV GGNNCC 3 cut(s) 198, 649, 650
PasI CCCWGGG 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 217
PkrI GCNGC 7 cut(s) 16, 438, 523, 556, 559, 582, 666
PleI GAGTC 3 cut(s) 137, 349, 473
PpsI GAGTC 3 cut(s) 137, 349, 473
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 651
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 3 cut(s) 198, 649, 650
PspOMI GGGCCC 1 cut(s) 647
PspPI GGNCC 3 cut(s) 398, 647, 648
PsuI RGATCY 3 cut(s) 290, 592, 802
PvuII CAGCTG 2 cut(s) 230, 436
RsaI GTAC 2 cut(s) 460, 953
RsaNI GTAC 2 cut(s) 459, 952
SaqAI TTAA 3 cut(s) 576, 794, 873
SatI GCNGC 7 cut(s) 15, 437, 522, 555, 558, 581, 665
Sau3AI GATC 3 cut(s) 290, 592, 802
Sau96I GGNCC 3 cut(s) 398, 647, 648
SchI GAGTC 3 cut(s) 137, 350, 473
ScrFI CCNGG 2 cut(s) 219, 653
SduI GDGCHC 1 cut(s) 651
SetI ASST 9 cut(s) 59, 129, 202, 232, 274, 438, 474, 585, 634
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 1 cut(s) 454
SinI GGWCC 1 cut(s) 398
Sse9I AATT 7 cut(s) 211, 317, 694, 723, 828, 996, 1058
SsiI CCGC 5 cut(s) 506, 557, 665, 921, 1035
SspMI CTAG 2 cut(s) 338, 596
StyD4I CCNGG 2 cut(s) 217, 651
TaaI ACNGT 3 cut(s) 64, 388, 958
TaiI ACGT 2 cut(s) 59, 474
TaqI TCGA 3 cut(s) 235, 420, 860
TasI AATT 7 cut(s) 211, 317, 694, 723, 828, 996, 1058
TatI WGTACW 1 cut(s) 951
TauI GCSGC 2 cut(s) 560, 667
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 3 cut(s) 576, 794, 873
Tru9I TTAA 3 cut(s) 576, 794, 873
TscAI CASTG 5 cut(s) 69, 303, 393, 547, 961
TseI GCWGC 5 cut(s) 14, 436, 521, 554, 580
TspDTI ATGAA 6 cut(s) 177, 365, 675, 777, 924, 1038
TspRI CASTG 5 cut(s) 69, 303, 393, 547, 961
VpaK11BI GGWCC 1 cut(s) 398
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 1 cut(s) 317
XbaI TCTAGA 1 cut(s) 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 2 cut(s) 338, 596
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.