Rh3CG222400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001140
Physical Location & Seq
Reverse (-)
20749128 .. 20753243
4116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG222400.1

Sequence Viewer

Length: 393 bp
ATGGAATTAGATGGAAGAATATTATTCCTTATTCTCTTCGTCTTTTACTGCGTCTCAGCTCTTATAGGGGAAGGAAAGGCGCCGCTAGTTGGAGCTGTTCCCCACCCCTGGCGTCCAACAAAGCTCTTTGTTTTCGGAGACTCCTACGCTGCCACCGGCAACAATGTCGCGAGCCTTGGCGTTGATTCTGACCGCTGTTGGATATCTCCTTATGGAGATACCTTTCCCGGCAAACCCACTGGCTGTTTCTCCGATGGTCGTGTCCTCACTGATTTCATTGGGGACTGGTGCAGATGCAAAGGTCCGATCATGGCAGGGACGAGGTTTCAGTACCCAGTGGGAGATAGCGATGGCGGCAACATGAAGCTGCATATTCTAAGAAGAGATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

130

Amino Acids

14.17

Weight (kDa)

6.69

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccII CGCG 1 cut(s) 170
AciI CCGC 3 cut(s) 83, 193, 354
AcyI GRCGYC 2 cut(s) 80, 112
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 2 cut(s) 89, 108
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 4 cut(s) 59, 95, 124, 367
AluI AGCT 4 cut(s) 59, 95, 124, 367
Alw26I GTCTC 2 cut(s) 58, 132
ApeKI GCWGC 2 cut(s) 149, 367
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 1 cut(s) 228
AvaII GGWCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 136, 354
BccI CCATC 4 cut(s) 5, 248, 344, 380
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 58, 132
BfaI CTAG 1 cut(s) 86
BfoI RGCGCY 1 cut(s) 83
BisI GCNGC 4 cut(s) 83, 150, 355, 368
BlsI GCNGC 4 cut(s) 84, 151, 356, 369
Bme1390I CCNGG 2 cut(s) 109, 228
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 109, 228
BmrI ACTGGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 228
BsaHI GRCGYC 2 cut(s) 80, 112
BsaJI CCNNGG 2 cut(s) 107, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 108
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 244, 290, 335
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 107, 175
BseLI CCNNNNNNNGG 2 cut(s) 89, 108
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 3 cut(s) 244, 290, 335
BseXI GCAGC 2 cut(s) 136, 354
BsgI GTGCAG 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 170
BshNI GGYRCC 1 cut(s) 79
BsiSI CCGG 2 cut(s) 156, 228
BslFI GGGAC 2 cut(s) 296, 331
BslI CCNNNNNNNGG 2 cut(s) 89, 108
BsmAI GTCTC 2 cut(s) 58, 132
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 296, 331
Bsp143I GATC 1 cut(s) 306
Bsp68I TCGCGA 1 cut(s) 170
BspACI CCGC 3 cut(s) 83, 193, 354
BspCNI CTCAG 1 cut(s) 68
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 3 cut(s) 244, 290, 335
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 107, 175
BssMI GATC 1 cut(s) 306
BssNI GRCGYC 2 cut(s) 80, 112
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 109
Bst6I CTCTTC 2 cut(s) 41, 376
BstACI GRCGYC 2 cut(s) 80, 112
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 55, 377
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 2 cut(s) 58, 132
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 107, 226
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 2 cut(s) 136, 354
BtgZI GCGATG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 237, 267, 342
BtuMI TCGCGA 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 82
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 302
CseI GACGC 2 cut(s) 40, 101
Csp6I GTAC 1 cut(s) 331
CviAII CATG 2 cut(s) 310, 361
CviJI RGCY 7 cut(s) 59, 95, 124, 174, 243, 367, 390
CviKI_1 RGCY 7 cut(s) 59, 95, 124, 174, 243, 367, 390
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 2 cut(s) 55, 377
DinI GGCGCC 1 cut(s) 81
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 41, 376
EarI CTCTTC 2 cut(s) 41, 376
Eco130I CCWWGG 1 cut(s) 175
Eco32I GATATC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 107
EcoRV GATATC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 175
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 2 cut(s) 313, 364
FaiI YATR 5 cut(s) 65, 213, 311, 362, 372
FaqI GGGAC 2 cut(s) 296, 331
FatI CATG 2 cut(s) 309, 360
Fnu4HI GCNGC 4 cut(s) 83, 150, 355, 368
Fsp4HI GCNGC 4 cut(s) 83, 150, 355, 368
FspBI CTAG 1 cut(s) 86
GlaI GCGC 1 cut(s) 81
GluI GCNGC 4 cut(s) 83, 150, 355, 368
HaeII RGCGCY 1 cut(s) 83
HapII CCGG 2 cut(s) 156, 228
HgaI GACGC 2 cut(s) 40, 101
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 2 cut(s) 80, 112
Hin1II CATG 2 cut(s) 313, 364
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 2 cut(s) 140, 185
HpaII CCGG 2 cut(s) 156, 228
Hpy188I TCNGA 4 cut(s) 137, 190, 253, 306
Hpy188III TCNNGA 1 cut(s) 169
HpyAV CCTTC 1 cut(s) 65
HpyCH4V TGCA 3 cut(s) 291, 297, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 2 cut(s) 55, 377
Hsp92I GRCGYC 2 cut(s) 80, 112
Hsp92II CATG 2 cut(s) 313, 364
HspAI GCGC 1 cut(s) 80
KasI GGCGCC 1 cut(s) 79
Kzo9I GATC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 92
LpnPI CCDG 8 cut(s) 94, 121, 169, 225, 241, 271, 300, 348
Lsp1109I GCAGC 2 cut(s) 136, 354
LweI GCATC 1 cut(s) 284
MaeI CTAG 1 cut(s) 86
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 3 cut(s) 27, 28, 393
MluCI AATT 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 3 cut(s) 70, 140, 179
MnlI CCTC 2 cut(s) 275, 315
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 2 cut(s) 156, 228
MspR9I CCNGG 2 cut(s) 109, 228
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 80
NciI CCSGG 1 cut(s) 228
NdeII GATC 1 cut(s) 306
NlaIII CATG 2 cut(s) 313, 364
NlaIV GGNNCC 1 cut(s) 81
NruI TCGCGA 1 cut(s) 170
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 4 cut(s) 84, 151, 356, 369
PleI GAGTC 1 cut(s) 134
PluTI GGCGCC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 1 cut(s) 302
RruI TCGCGA 1 cut(s) 170
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SatI GCNGC 4 cut(s) 83, 150, 355, 368
Sau3AI GATC 1 cut(s) 306
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 109, 228
SetI ASST 7 cut(s) 61, 97, 126, 224, 304, 326, 369
SfaNI GCATC 1 cut(s) 284
SfoI GGCGCC 1 cut(s) 81
SinI GGWCC 1 cut(s) 302
Sse9I AATT 1 cut(s) 5
SsiI CCGC 3 cut(s) 83, 193, 354
SspDI GGCGCC 1 cut(s) 79
SspI AATATT 1 cut(s) 21
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 107, 226
StyI CCWWGG 1 cut(s) 175
TasI AATT 1 cut(s) 5
TauI GCSGC 2 cut(s) 85, 357
TfiI GAWTC 1 cut(s) 185
TscAI CASTG 3 cut(s) 244, 274, 342
TseI GCWGC 2 cut(s) 149, 367
TspDTI ATGAA 2 cut(s) 265, 377
TspRI CASTG 3 cut(s) 244, 274, 342
VpaK11BI GGWCC 1 cut(s) 302
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.