Rw3G016240

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001140
Physical Location & Seq
Forward (+)
17013734 .. 17031588
17855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016240.1

Sequence Viewer

Length: 774 bp
ATGGATCTGCATATTATGCCTCTCTTTCCTTTTAGATCAATTTTCATTAAGGAAAGGCGCCGCTACTCGCTAGTTGGAGCTGTTCCCCACCCCTGGCGTCCAACAAAGCTCTTTGTTTTCGGAGACTCCTACGCCGCCACCGGCAACAATGTCGCGAGCCTTGGCGTTGATTCTGACCGCTGTTGGATATCTCCTTATGGAGATACCTTTCCCGGCAAACCCACTGGCCGTTTCTCCGATGGTCGTGTCCTCACTGATTTCATTGCAGTGAAGTCTCCACTGGCACACAAATTTAGTAGTGCTGCAGGCAACTATTTGAAATATGGGATGAATTTCGCATATGGAGGCAGTTCGGGCTCTGTATACAACACTACAGACCTCTTTTCCTCTGTAGCCCTTGTCACTGATTTGAAACTTATTTATGACTTTGGAGTGAAGAAATTACTTGTGACAAATTTGGCACCTATGGGATGCCTTCCTAGGATAACTTCTTCCCAAAACCTCTTATTGCAGCAAGCTGTGGCCAAGTTGAACACTGAAACCGAGAATTCTTCCTTCATCGTTCTCGACCATTATGCCTTGTTTATGTTAGTTTTCAAGAACAAAGGAGATCATCTAGGACGGATAAAGTTTGACAACCCATTGAGCCAGTGTTGTATTGGTGTAAGTAATGGATATTCATTTGGGAATCAGGATGATATGACCGGAGAACCTAGGTACACAATCTGCGAGGGTAATTCCGCATTCTTTTGGCACGATACTCACCCTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

257

Amino Acids

28.5

Weight (kDa)

8.53

Isoelectric Point (pI)

31.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 57, 460
AccI GTMKAC 1 cut(s) 363
AccII CGCG 1 cut(s) 155
AciI CCGC 4 cut(s) 61, 135, 178, 741
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 226, 522
AcsI RAATTY 4 cut(s) 290, 331, 454, 547
AcyI GRCGYC 2 cut(s) 58, 97
AfaI GTAC 1 cut(s) 719
AfiI CCNNNNNNNGG 1 cut(s) 93
AgsI TTSAA 4 cut(s) 319, 412, 532, 598
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 3 cut(s) 80, 109, 518
AluI AGCT 3 cut(s) 80, 109, 518
Alw26I GTCTC 2 cut(s) 117, 279
AlwI GGATC 1 cut(s) 12
AoxI GGCC 2 cut(s) 226, 522
ApeKI GCWGC 2 cut(s) 302, 511
ApoI RAATTY 4 cut(s) 290, 331, 454, 547
AspA2I CCTAGG 2 cut(s) 479, 713
AspLEI GCGC 1 cut(s) 60
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 755
AvrII CCTAGG 2 cut(s) 479, 713
BalI TGGCCA 1 cut(s) 524
BanI GGYRCC 2 cut(s) 57, 460
BanII GRGCYC 1 cut(s) 359
BbvI GCAGC 2 cut(s) 289, 523
BccI CCATC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 4 cut(s) 133, 167, 557, 591
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 117, 279
BfaI CTAG 4 cut(s) 71, 480, 617, 714
BfmI CTRYAG 3 cut(s) 303, 372, 390
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 4 cut(s) 61, 135, 303, 512
BlnI CCTAGG 2 cut(s) 479, 713
BlsI GCNGC 4 cut(s) 62, 136, 304, 513
Bme1390I CCNGG 2 cut(s) 94, 213
BmiI GGNNCC 2 cut(s) 59, 462
BmrFI CCNGG 2 cut(s) 94, 213
BmsI GCATC 1 cut(s) 461
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 2 cut(s) 58, 97
BsaJI CCNNGG 4 cut(s) 92, 160, 479, 713
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 1 cut(s) 93
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 3 cut(s) 229, 285, 649
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 4 cut(s) 92, 160, 479, 713
BseGI GGATG 3 cut(s) 333, 476, 700
BseLI CCNNNNNNNGG 1 cut(s) 93
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 3 cut(s) 229, 285, 649
BseXI GCAGC 2 cut(s) 289, 523
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 228, 524
BshNI GGYRCC 2 cut(s) 57, 460
BsiSI CCGG 3 cut(s) 141, 213, 705
BslI CCNNNNNNNGG 1 cut(s) 93
BsmAI GTCTC 2 cut(s) 117, 279
BsmI GAATGC 1 cut(s) 743
BsnI GGCC 2 cut(s) 228, 524
Bsp1286I GDGCHC 1 cut(s) 359
Bsp143I GATC 3 cut(s) 4, 35, 610
Bsp68I TCGCGA 1 cut(s) 155
BspACI CCGC 4 cut(s) 61, 135, 178, 741
BspANI GGCC 2 cut(s) 228, 524
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 59, 462
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 57, 460
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 3 cut(s) 229, 285, 649
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 4 cut(s) 92, 160, 479, 713
BssMI GATC 3 cut(s) 4, 35, 610
BssNAI GTATAC 1 cut(s) 364
BssNI GRCGYC 2 cut(s) 58, 97
BssT1I CCWWGG 3 cut(s) 160, 479, 713
Bst1107I GTATAC 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 94
BstACI GRCGYC 2 cut(s) 58, 97
BstAPI GCANNNNNTGC 1 cut(s) 16
BstC8I GCNNGC 3 cut(s) 157, 307, 516
BstDEI CTNAG 1 cut(s) 771
BstF5I GGATG 3 cut(s) 333, 476, 700
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 3 cut(s) 7, 38, 613
BstMAI GTCTC 2 cut(s) 117, 279
BstMBI GATC 3 cut(s) 4, 35, 610
BstMWI GCNNNNNNNGC 2 cut(s) 16, 354
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 92, 211
BstSFI CTRYAG 3 cut(s) 303, 372, 390
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 289, 523
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BstZ17I GTATAC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 228, 524
BtsCI GGATG 3 cut(s) 333, 476, 700
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 7 cut(s) 222, 252, 273, 278, 402, 534, 656
BtuMI TCGCGA 1 cut(s) 155
Cac8I GCNNGC 3 cut(s) 157, 307, 516
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 140
CseI GACGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 718
CviJI RGCY 9 cut(s) 80, 109, 159, 228, 357, 395, 518, 524, 648
CviKI_1 RGCY 9 cut(s) 80, 109, 159, 228, 357, 395, 518, 524, 648
CviQI GTAC 1 cut(s) 718
DdeI CTNAG 1 cut(s) 771
DinI GGCGCC 1 cut(s) 59
DpnI GATC 3 cut(s) 6, 37, 612
DpnII GATC 3 cut(s) 4, 35, 610
EaeI YGGCCR 2 cut(s) 226, 522
Eco130I CCWWGG 3 cut(s) 160, 479, 713
Eco24I GRGCYC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 92
EcoRV GATATC 1 cut(s) 189
EcoT14I CCWWGG 3 cut(s) 160, 479, 713
EcoT38I GRGCYC 1 cut(s) 359
EgeI GGCGCC 1 cut(s) 59
EheI GGCGCC 1 cut(s) 59
ErhI CCWWGG 3 cut(s) 160, 479, 713
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 61, 135, 303, 512
FokI GGATG 3 cut(s) 340, 483, 707
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 61, 135, 303, 512
FspBI CTAG 4 cut(s) 71, 480, 617, 714
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 61, 135, 303, 512
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 2 cut(s) 228, 524
HapII CCGG 3 cut(s) 141, 213, 705
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 2 cut(s) 58, 97
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 3 cut(s) 125, 170, 688
HpaII CCGG 3 cut(s) 141, 213, 705
HphI GGTGA 1 cut(s) 755
Hpy166II GTNNAC 2 cut(s) 364, 720
Hpy188I TCNGA 3 cut(s) 122, 175, 238
Hpy188III TCNNGA 4 cut(s) 154, 566, 598, 692
Hpy8I GTNNAC 2 cut(s) 364, 720
HpyAV CCTTC 2 cut(s) 485, 565
HpyCH4V TGCA 4 cut(s) 10, 266, 305, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 354
HpyF3I CTNAG 1 cut(s) 771
Hsp92I GRCGYC 2 cut(s) 58, 97
HspAI GCGC 1 cut(s) 58
KasI GGCGCC 1 cut(s) 57
Kzo9I GATC 3 cut(s) 4, 35, 610
LmnI GCTCC 1 cut(s) 77
Lsp1109I GCAGC 2 cut(s) 289, 523
LweI GCATC 1 cut(s) 461
MaeI CTAG 4 cut(s) 71, 480, 617, 714
MaeIII GTNAC 2 cut(s) 400, 448
MalI GATC 3 cut(s) 6, 37, 612
MboI GATC 3 cut(s) 4, 35, 610
MboII GAAGA 3 cut(s) 448, 483, 543
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 7 cut(s) 39, 290, 331, 440, 454, 547, 736
MluNI TGGCCA 1 cut(s) 524
Mly113I GGCGCC 1 cut(s) 58
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 3 cut(s) 55, 125, 164
MnlI CCTC 7 cut(s) 30, 260, 338, 389, 397, 512, 724
Mox20I TGGCCA 1 cut(s) 524
MscI TGGCCA 1 cut(s) 524
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 180
MspI CCGG 3 cut(s) 141, 213, 705
MspR9I CCNGG 2 cut(s) 94, 213
Mva1269I GAATGC 1 cut(s) 743
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 2 cut(s) 16, 354
NarI GGCGCC 1 cut(s) 58
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 340
NdeII GATC 3 cut(s) 4, 35, 610
NlaIV GGNNCC 2 cut(s) 59, 462
NmuCI GTSAC 2 cut(s) 400, 448
NruI TCGCGA 1 cut(s) 155
PctI GAATGC 1 cut(s) 743
PfeI GAWTC 2 cut(s) 170, 688
PkrI GCNGC 4 cut(s) 62, 136, 304, 513
PleI GAGTC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 59, 462
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 4
RruI TCGCGA 1 cut(s) 155
RsaI GTAC 1 cut(s) 719
RsaNI GTAC 1 cut(s) 718
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 4 cut(s) 61, 135, 303, 512
Sau3AI GATC 3 cut(s) 4, 35, 610
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 94, 213
SduI GDGCHC 1 cut(s) 359
SetI ASST 9 cut(s) 82, 111, 209, 381, 466, 504, 520, 715, 719
SfaNI GCATC 1 cut(s) 461
SfcI CTRYAG 3 cut(s) 303, 372, 390
SfoI GGCGCC 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 266
Sse9I AATT 7 cut(s) 39, 290, 331, 440, 454, 547, 736
SsiI CCGC 4 cut(s) 61, 135, 178, 741
SspDI GGCGCC 1 cut(s) 57
SspMI CTAG 4 cut(s) 71, 480, 617, 714
StyD4I CCNGG 2 cut(s) 92, 211
StyI CCWWGG 3 cut(s) 160, 479, 713
TaqI TCGA 1 cut(s) 567
TasI AATT 7 cut(s) 39, 290, 331, 440, 454, 547, 736
TauI GCSGC 2 cut(s) 63, 137
TfiI GAWTC 2 cut(s) 170, 688
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 7 cut(s) 229, 259, 273, 285, 409, 541, 656
TseFI GTSAC 2 cut(s) 400, 448
TseI GCWGC 2 cut(s) 302, 511
Tsp45I GTSAC 2 cut(s) 400, 448
TspDTI ATGAA 5 cut(s) 34, 250, 344, 547, 669
TspGWI ACGGA 1 cut(s) 637
TspRI CASTG 7 cut(s) 229, 259, 273, 285, 409, 541, 656
XapI RAATTY 4 cut(s) 290, 331, 454, 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 656
XmaJI CCTAGG 2 cut(s) 479, 713
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 4 cut(s) 71, 480, 617, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.