FvH4_6g18330

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001140
Physical Location & Seq
Reverse (-)
12107321 .. 12108831
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18330.t1

Sequence Viewer

Length: 897 bp
ATGGAATTAGATGGAAAAATCCTGTTCCTCATTCTCTCTGTCTTTTACTCCGTCTCAGGTCTTATTGGAGAAGGAGAGGTGCTACTAGTTGATGCTGTTCCCCACCTTTGGCGTCCAACAAAGCTCTTTGTTTTCGGAGACTCCTCCGCTGCCACCGGCAACAATGTCGCGAGTCTTGGCTCTGATTCTGACATCTGTTGGCATTCTCCTTATGGAGAAACCTTTCCCGGAAAACCCACTGCCCGGTTCTCCGATGGTCGCGTCCTCACTGATTTCATCGCTAAGTTCATAGCAGTGAAGTCTCCTCTGGCATACAAAATTAGAAATGCTGCAGGCTACTATTCGAAATATGGGATGAATTTCGCATATGGAGGCCATTCGAGGAATGAAGACTCTGTATACAACACAACAGACCTTTTTTCCTCTGTTGCCCTCGTCACTGTTTCCGGCGATGACAACAACTATAACTATTTCAACGAAGGATTTGTTAAGGATTTGAAACGTATTTACGACTTTGGCGTGAAGAAAATACTTGTGACAAATCTGGCACCTATGGGATGCCTTCCTAGAATCACATCTCAAGCTGTGGCCAAGCTGAACAGTGAAACCGAGAGTTCTTCGTTCATCATTCTTGACCTTTATGCCTCGTTTACGCCAGTTTTCAAGAACAAAGCAGACCATCTAGGAAGCATAAAGTTTAAGAAGCCACTGAAGTCGTGTTGTGTTGGACAAAGTGATTCAGATTGGTGTGGTCGTAGGTATGATATGGACGGGAGCCCTATGTATACCGTCTGTGAGCGTAACTCTGCTTTCTTTTGGGACGACGCTCACCCTTCCCAGCAGGGCTGGACCGCTGTGTATTCCAACTTGCAAGCTACGCTTCAGCAACTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

299

Amino Acids

33.05

Weight (kDa)

6.21

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 286 2.2e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 2 cut(s) 399, 785
AccII CGCG 2 cut(s) 170, 261
AciI CCGC 2 cut(s) 147, 852
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 2 cut(s) 731, 866
AcyI GRCGYC 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 108, 243
AgsI TTSAA 3 cut(s) 475, 499, 664
AhlI ACTAGT 1 cut(s) 85
AluBI AGCT 4 cut(s) 124, 584, 595, 875
AluI AGCT 4 cut(s) 124, 584, 595, 875
Alw26I GTCTC 3 cut(s) 58, 132, 306
AoxI GGCC 2 cut(s) 373, 588
ApeKI GCWGC 2 cut(s) 149, 329
ApoI RAATTY 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 849
AsuC2I CCSGG 2 cut(s) 228, 244
AsuHPI GGTGA 1 cut(s) 821
AsuII TTCGAA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 849
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 396
BbvI GCAGC 2 cut(s) 136, 316
BccI CCATC 3 cut(s) 5, 248, 687
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BcnI CCSGG 2 cut(s) 228, 244
BcoDI GTCTC 3 cut(s) 58, 132, 306
BcuI ACTAGT 1 cut(s) 85
BfaI CTAG 3 cut(s) 86, 567, 683
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 2 cut(s) 150, 330
BlsI GCNGC 2 cut(s) 151, 331
Bme1390I CCNGG 2 cut(s) 228, 244
Bme18I GGWCC 1 cut(s) 849
BmgT120I GGNCC 1 cut(s) 849
BmiI GGNNCC 2 cut(s) 549, 776
BmrFI CCNGG 2 cut(s) 228, 244
BmsI GCATC 2 cut(s) 82, 548
BpiI GAAGAC 1 cut(s) 396
BplI GAGNNNNNCTC 2 cut(s) 788, 820
Bpu14I TTCGAA 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 564
BpuMI CCSGG 2 cut(s) 228, 244
BsaHI GRCGYC 1 cut(s) 112
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 243
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 656
BseGI GGATG 2 cut(s) 360, 563
BseLI CCNNNNNNNGG 2 cut(s) 108, 243
BseMII CTCAG 1 cut(s) 69
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 2 cut(s) 133, 294
BseXI GCAGC 2 cut(s) 136, 316
BseYI CCCAGC 1 cut(s) 837
Bsh1236I CGCG 2 cut(s) 170, 261
BshFI GGCC 2 cut(s) 375, 590
BshNI GGYRCC 1 cut(s) 547
BsiSI CCGG 4 cut(s) 156, 228, 244, 447
BslFI GGGAC 1 cut(s) 833
BslI CCNNNNNNNGG 2 cut(s) 108, 243
BsmAI GTCTC 3 cut(s) 58, 132, 306
BsmBI CGTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 833
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 2 cut(s) 375, 590
Bsp119I TTCGAA 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 779
Bsp68I TCGCGA 1 cut(s) 170
BspACI CCGC 2 cut(s) 147, 852
BspANI GGCC 2 cut(s) 375, 590
BspCNI CTCAG 1 cut(s) 68
BspFNI CGCG 2 cut(s) 170, 261
BspLI GGNNCC 2 cut(s) 549, 776
BspMAI CTGCAG 1 cut(s) 334
BspT104I TTCGAA 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 1 cut(s) 155
BssNAI GTATAC 2 cut(s) 400, 786
BssNI GRCGYC 1 cut(s) 112
Bst1107I GTATAC 2 cut(s) 400, 786
Bst4CI ACNGT 3 cut(s) 442, 602, 790
BstACI GRCGYC 1 cut(s) 112
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 334, 873
BstDEI CTNAG 2 cut(s) 55, 282
BstF5I GGATG 2 cut(s) 360, 563
BstFNI CGCG 2 cut(s) 170, 261
BstMAI GTCTC 3 cut(s) 58, 132, 306
BstMWI GCNNNNNNNGC 1 cut(s) 877
BstSCI CCNGG 2 cut(s) 226, 242
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 2 cut(s) 170, 261
BstV1I GCAGC 2 cut(s) 136, 316
BstV2I GAAGAC 1 cut(s) 396
BstZ17I GTATAC 2 cut(s) 400, 786
BsuRI GGCC 2 cut(s) 375, 590
BtgZI GCGATG 2 cut(s) 262, 465
BtsCI GGATG 2 cut(s) 360, 563
BtsI GCAGTG 2 cut(s) 237, 300
BtsIMutI CAGTG 6 cut(s) 237, 267, 300, 438, 607, 707
BtuMI TCGCGA 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 334, 873
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 849
CseI GACGC 3 cut(s) 101, 250, 833
DdeI CTNAG 2 cut(s) 55, 282
EaeI YGGCCR 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 779
Eco47I GGWCC 1 cut(s) 849
Eco57I CTGAAG 2 cut(s) 731, 866
EcoT38I GRGCYC 1 cut(s) 779
Esp3I CGTCTC 1 cut(s) 58
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FaqI GGGAC 1 cut(s) 833
FauNDI CATATG 1 cut(s) 367
FblI GTMKAC 2 cut(s) 399, 785
Fnu4HI GCNGC 2 cut(s) 150, 330
FokI GGATG 2 cut(s) 367, 570
FriOI GRGCYC 1 cut(s) 779
Fsp4HI GCNGC 2 cut(s) 150, 330
FspBI CTAG 3 cut(s) 86, 567, 683
GluI GCNGC 2 cut(s) 150, 330
GsaI CCCAGC 1 cut(s) 841
HaeIII GGCC 2 cut(s) 375, 590
HapII CCGG 4 cut(s) 156, 228, 244, 447
HgaI GACGC 3 cut(s) 101, 250, 833
Hin1I GRCGYC 1 cut(s) 112
HinfI GANTC 6 cut(s) 140, 172, 185, 392, 570, 737
HpaII CCGG 4 cut(s) 156, 228, 244, 447
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 3 cut(s) 400, 651, 786
Hpy188I TCNGA 5 cut(s) 137, 184, 190, 253, 742
Hpy188III TCNNGA 3 cut(s) 169, 632, 664
Hpy8I GTNNAC 3 cut(s) 400, 651, 786
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 4 cut(s) 65, 473, 572, 843
HpyCH4III ACNGT 3 cut(s) 442, 602, 790
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 2 cut(s) 332, 871
HpyF10VI GCNNNNNNNGC 1 cut(s) 877
HpyF3I CTNAG 2 cut(s) 55, 282
HpySE526I ACGT 1 cut(s) 502
Hsp92I GRCGYC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 774
Lsp1109I GCAGC 2 cut(s) 136, 316
LweI GCATC 2 cut(s) 82, 548
MaeI CTAG 3 cut(s) 86, 567, 683
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 3 cut(s) 436, 535, 800
MboII GAAGA 3 cut(s) 401, 535, 609
MhlI GDGCHC 1 cut(s) 779
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 3 cut(s) 5, 318, 358
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 3 cut(s) 134, 181, 386
MmeI TCCRAC 3 cut(s) 140, 706, 888
Mox20I TGGCCA 1 cut(s) 590
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 1 cut(s) 590
MseI TTAA 2 cut(s) 489, 699
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 2 cut(s) 149, 854
MspI CCGG 4 cut(s) 156, 228, 244, 447
MspR9I CCNGG 2 cut(s) 228, 244
Mva1269I GAATGC 1 cut(s) 202
MvnI CGCG 2 cut(s) 170, 261
MwoI GCNNNNNNNGC 1 cut(s) 877
NciI CCSGG 2 cut(s) 228, 244
NdeI CATATG 1 cut(s) 367
NlaIV GGNNCC 2 cut(s) 549, 776
NmuCI GTSAC 2 cut(s) 436, 535
NruI TCGCGA 1 cut(s) 170
NspV TTCGAA 1 cut(s) 344
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 3 cut(s) 185, 570, 737
PfoI TCCNGGA 1 cut(s) 226
PkrI GCNGC 2 cut(s) 151, 331
PleI GAGTC 3 cut(s) 134, 180, 386
PpsI GAGTC 3 cut(s) 134, 180, 386
PspFI CCCAGC 1 cut(s) 837
PspN4I GGNNCC 2 cut(s) 549, 776
PspPI GGNCC 1 cut(s) 849
PstI CTGCAG 1 cut(s) 334
RruI TCGCGA 1 cut(s) 170
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 2 cut(s) 489, 699
SatI GCNGC 2 cut(s) 150, 330
Sau96I GGNCC 1 cut(s) 849
SchI GAGTC 3 cut(s) 134, 181, 386
ScrFI CCNGG 2 cut(s) 228, 244
SduI GDGCHC 1 cut(s) 779
SfaNI GCATC 2 cut(s) 82, 548
SfcI CTRYAG 1 cut(s) 330
SfuI TTCGAA 1 cut(s) 344
SinI GGWCC 1 cut(s) 849
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
SpeI ACTAGT 1 cut(s) 85
Sse9I AATT 3 cut(s) 5, 318, 358
SsiI CCGC 2 cut(s) 147, 852
SspMI CTAG 3 cut(s) 86, 567, 683
StyD4I CCNGG 2 cut(s) 226, 242
TaaI ACNGT 3 cut(s) 442, 602, 790
TaiI ACGT 1 cut(s) 505
TaqI TCGA 2 cut(s) 344, 380
TasI AATT 3 cut(s) 5, 318, 358
TfiI GAWTC 3 cut(s) 185, 570, 737
Tru1I TTAA 2 cut(s) 489, 699
Tru9I TTAA 2 cut(s) 489, 699
TscAI CASTG 6 cut(s) 244, 274, 300, 445, 607, 714
TseFI GTSAC 2 cut(s) 436, 535
TseI GCWGC 2 cut(s) 149, 329
Tsp45I GTSAC 2 cut(s) 436, 535
TspDTI ATGAA 5 cut(s) 265, 277, 371, 402, 613
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 6 cut(s) 244, 274, 300, 445, 607, 714
VpaK11BI GGWCC 1 cut(s) 849
XapI RAATTY 1 cut(s) 358
XmiI GTMKAC 2 cut(s) 399, 785
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 3 cut(s) 86, 567, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.