Prupe.2G080800_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001140
Physical Location & Seq
Reverse (-)
12442424 .. 12445071
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G080800.1

Sequence Viewer

Length: 594 bp
ATGAGTTCATGTGACATTTCTAAAATTTTACTTAACTATTATTGTACTGCACAATTATCATACCTTCAGGATTTCGCAGTGGTCACTAAATTCATTATTAAGCAGCTTGCTGTGGATCTGGAGCGTATATATGGGTTGGGAGTGGGAAAAATAGCTGTTACAGCAATAGGGCCCCTGGGATGTCTGCCCCGCATGACTTCCTTTCTTTCATATCAGAATTGTAGTGAAGTTGCCAATCTGGTTTCAATTTTCCATAACCAGATTTTGCGTCAAAAAGTTGAAGAGTTGAACAAGGAGACTAAGAAATCTTCATTTGTAATCTTGGATCTTTATAACGCGTCTCTGTCTGCAATTATGCTCCCAAAACACCATCAAGGATATTCGACACTCCAGATTAATCCACTAAAGCCGTGCTGTGTTGGGGTGAGCAATGAATACTCATGCGGGAGTGTGGATGAGAGTGGAGCAAAGAAGTACACTGTATGCGATAAGCCCAAACTTTCTTTCTTTTGGGACACGATTCACCCTTCGCAGAATGGCTGGCATGAAGTTTATTCCGCTATAAAATCTTCTCTTCGTCAATTGTATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

198

Amino Acids

22.07

Weight (kDa)

8.56

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AccII CGCG 1 cut(s) 338
AciI CCGC 3 cut(s) 190, 444, 558
AclWI GGATC 2 cut(s) 123, 333
AcsI RAATTY 2 cut(s) 24, 89
AcuI CTGAAG 1 cut(s) 50
AfaI GTAC 2 cut(s) 46, 476
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 246, 281, 289
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 2 cut(s) 106, 155
AluI AGCT 2 cut(s) 106, 155
Alw26I GTCTC 2 cut(s) 290, 345
AlwI GGATC 2 cut(s) 123, 333
AoxI GGCC 1 cut(s) 170
ApaI GGGCCC 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 103
ApoI RAATTY 2 cut(s) 24, 89
AseI ATTAAT 1 cut(s) 396
AspS9I GGNCC 2 cut(s) 170, 171
AsuHPI GGTGA 2 cut(s) 436, 515
BaeGI GKGCMC 1 cut(s) 174
BanII GRGCYC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 115
BccI CCATC 1 cut(s) 378
BceAI ACGGC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 176
BcoDI GTCTC 2 cut(s) 290, 345
BisI GCNGC 1 cut(s) 104
BlsI GCNGC 1 cut(s) 105
Bme1390I CCNGG 1 cut(s) 176
BmgT120I GGNCC 2 cut(s) 170, 171
BmiI GGNNCC 2 cut(s) 172, 173
BmrFI CCNGG 1 cut(s) 176
BpmI CTGGAG 2 cut(s) 140, 374
BsaJI CCNNGG 2 cut(s) 174, 175
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 174, 175
BseGI GGATG 2 cut(s) 185, 460
BseMI GCAATG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 1 cut(s) 172
BslFI GGGAC 1 cut(s) 527
BsmAI GTCTC 2 cut(s) 290, 345
BsmBI CGTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 1 cut(s) 172
Bsp120I GGGCCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 115, 325
BspACI CCGC 3 cut(s) 190, 444, 558
BspANI GGCC 1 cut(s) 172
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 2 cut(s) 172, 173
BspPI GGATC 2 cut(s) 123, 333
BsrDI GCAATG 1 cut(s) 436
BssECI CCNNGG 2 cut(s) 174, 175
BssMI GATC 2 cut(s) 115, 325
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 276, 579
BstC8I GCNNGC 2 cut(s) 108, 542
BstDEI CTNAG 1 cut(s) 300
BstF5I GGATG 2 cut(s) 185, 460
BstFNI CGCG 1 cut(s) 338
BstKTI GATC 2 cut(s) 118, 328
BstMAI GTCTC 2 cut(s) 290, 345
BstMBI GATC 2 cut(s) 115, 325
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 1 cut(s) 115
BstX2I RGATCY 2 cut(s) 115, 325
BstYI RGATCY 2 cut(s) 115, 325
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 2 cut(s) 185, 460
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 2 cut(s) 84, 477
Cac8I GCNNGC 2 cut(s) 108, 542
Cfr13I GGNCC 2 cut(s) 170, 171
CseI GACGC 2 cut(s) 257, 327
Csp6I GTAC 2 cut(s) 45, 475
CviAII CATG 4 cut(s) 9, 193, 441, 545
CviJI RGCY 6 cut(s) 106, 155, 172, 409, 493, 540
CviKI_1 RGCY 6 cut(s) 106, 155, 172, 409, 493, 540
CviQI GTAC 2 cut(s) 45, 475
DdeI CTNAG 1 cut(s) 300
DpnI GATC 2 cut(s) 117, 327
DpnII GATC 2 cut(s) 115, 325
Eam1104I CTCTTC 2 cut(s) 276, 579
EarI CTCTTC 2 cut(s) 276, 579
Eco24I GRGCYC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 50
EcoO109I RGGNCCY 2 cut(s) 170, 171
EcoRII CCWGG 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 174
Esp3I CGTCTC 1 cut(s) 345
FaeI CATG 4 cut(s) 12, 196, 444, 548
FaqI GGGAC 1 cut(s) 527
FatI CATG 4 cut(s) 8, 192, 440, 544
FauI CCCGC 2 cut(s) 197, 437
Fnu4HI GCNGC 1 cut(s) 104
FokI GGATG 2 cut(s) 192, 467
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 1 cut(s) 104
GluI GCNGC 1 cut(s) 104
GsuI CTGGAG 2 cut(s) 140, 374
HaeIII GGCC 1 cut(s) 172
HgaI GACGC 2 cut(s) 257, 327
Hin1II CATG 4 cut(s) 12, 196, 444, 548
HinfI GANTC 1 cut(s) 520
HphI GGTGA 2 cut(s) 436, 515
Hpy166II GTNNAC 1 cut(s) 477
Hpy188I TCNGA 1 cut(s) 216
Hpy188III TCNNGA 3 cut(s) 68, 119, 391
Hpy8I GTNNAC 1 cut(s) 477
HpyAV CCTTC 2 cut(s) 74, 537
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4V TGCA 2 cut(s) 50, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 300
Hsp92II CATG 4 cut(s) 12, 196, 444, 548
Kzo9I GATC 2 cut(s) 115, 325
LmnI GCTCC 3 cut(s) 121, 363, 464
LpnPI CCDG 8 cut(s) 53, 104, 161, 188, 224, 272, 404, 526
Lsp1109I GCAGC 1 cut(s) 115
MaeIII GTNAC 3 cut(s) 11, 82, 157
MalI GATC 2 cut(s) 117, 327
MboI GATC 2 cut(s) 115, 325
MboII GAAGA 4 cut(s) 293, 300, 561, 566
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 2 cut(s) 115, 325
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 7 cut(s) 24, 53, 89, 217, 246, 351, 581
MluI ACGCGT 1 cut(s) 336
MseI TTAA 3 cut(s) 33, 99, 396
MspR9I CCNGG 1 cut(s) 176
MunI CAATTG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 2 cut(s) 115, 325
NlaIII CATG 4 cut(s) 12, 196, 444, 548
NlaIV GGNNCC 2 cut(s) 172, 173
NmuCI GTSAC 2 cut(s) 11, 82
PasI CCCWGGG 1 cut(s) 175
PfeI GAWTC 1 cut(s) 520
PkrI GCNGC 1 cut(s) 105
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 172, 173
PspOMI GGGCCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 170, 171
PsuI RGATCY 2 cut(s) 115, 325
RsaI GTAC 2 cut(s) 46, 476
RsaNI GTAC 2 cut(s) 45, 475
SaqAI TTAA 3 cut(s) 33, 99, 396
SatI GCNGC 1 cut(s) 104
Sau3AI GATC 2 cut(s) 115, 325
Sau96I GGNCC 2 cut(s) 170, 171
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 1 cut(s) 174
SetI ASST 3 cut(s) 66, 108, 157
Sse9I AATT 7 cut(s) 24, 53, 89, 217, 246, 351, 581
SsiI CCGC 3 cut(s) 190, 444, 558
StyD4I CCNGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 481
TaqI TCGA 1 cut(s) 383
TasI AATT 7 cut(s) 24, 53, 89, 217, 246, 351, 581
TatI WGTACW 2 cut(s) 44, 474
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 3 cut(s) 33, 99, 396
Tru9I TTAA 3 cut(s) 33, 99, 396
TscAI CASTG 2 cut(s) 84, 484
TseFI GTSAC 2 cut(s) 11, 82
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 2 cut(s) 11, 82
TspDTI ATGAA 5 cut(s) 82, 198, 300, 447, 561
TspRI CASTG 2 cut(s) 84, 484
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 2 cut(s) 24, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.