AT3G09930

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001140
Physical Location & Seq
Forward (+)
3053237 .. 3055510
2274 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09930.1

Sequence Viewer

Length: 1065 bp
ATGGAGTTACCAAAACTATTAATCTCACTCTTTCTCTTCTCATTCTCATCTTTCTTCTTAGGTGCTGAATCGGATTATCCTCAGCATAGTAACTTGAAGAGACTGAGACCGAATAGACTGTTCGTGTTTGGAGATTCCTATGCAGACACCGGAAACATAAGGAAATCTCTCTCCGATTCTTGGAAAATTCCTTACGGCATCACTTTCCCCCAAAAACCTTCCGGTCGTTTCTCCGATGGCCGCGTCGCCACCGATTTTCTAGCAAGATACTTGGGGATAAAGTCACCAATTCCGTACACGTGGAAGGATTATGCCGGAAAGGAACGGCTATTGTACGGAATGAATTATGCGTATGGAGGAACAGGAGTGTTTAAGACTAAAGATAATCCATTGCCAAACATGACAACTCAGATTGATTATTTCCAACGTGTCCTCGCCGCCGGCAATATCTACTCGCCTTCCGATCTCCCCTCCTCCCTCGCTCTGGTCAGCGTCGCCGGCAACGACTACGCCACTTTCTTGGCCCTAAAACGCCCACTGACCGAGTTACCGGCATTCATGAAGCAAGTTGTTGATCAAATTGCGGTTAACGCCATGCGCATCCACAAGTTGGGAGTAAATAAAATAGTAATTCCTTCAATGCAGCCACTCGGATGCCTCCCTAGCATCACAGTCTTTAATTCATTCCAACGTTGCAACGCCACAGATAACGCATCAACGAATCTTCACAACTATTTGTTGCATAAAGCCATCGCTAGGCTTAATAATGAGACAAAGCCATCCACTTTCGTCGTTCTTGACCACTACAACGCGTTCTTGACTGTTTTCAAGAACAAAGGACCGGAACCTGGTGTTTCGAGGTTTGGGAACCCGTTAAAACCGTGTTGTGTCGGGGTCAACAGCAGTTACGACTGCTCAAATGTGGATGAGAAGGGTGAGAAGAAGTACATAATTTGTGAAGATCCAAAGGCTGCCTTCTTTTGGGACATCTTTCATCCGAGTGAAGAAGGATGGAGATCTGTTTACTCAGTTCTACACAAACATCTCAAAGCTATTTGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

354

Amino Acids

39.83

Weight (kDa)

9.24

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 40 - 338 9e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 599
AccB7I CCANNNNNTGG 1 cut(s) 610
AccII CGCG 2 cut(s) 243, 812
AciI CCGC 3 cut(s) 241, 438, 584
AclI AACGTT 1 cut(s) 691
AclWI GGATC 1 cut(s) 956
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 1 cut(s) 186
AcvI CACGTG 1 cut(s) 300
AfaI GTAC 3 cut(s) 296, 335, 947
AfiI CCNNNNNNNGG 6 cut(s) 180, 484, 610, 756, 848, 981
AflIII ACRYGT 3 cut(s) 297, 427, 810
AgsI TTSAA 3 cut(s) 97, 639, 829
AjnI CCWGG 1 cut(s) 847
AluBI AGCT 1 cut(s) 1052
AluI AGCT 1 cut(s) 1052
Alw26I GTCTC 3 cut(s) 94, 100, 764
AlwI GGATC 1 cut(s) 956
AoxI GGCC 2 cut(s) 238, 522
ApeKI GCWGC 2 cut(s) 643, 971
ApoI RAATTY 1 cut(s) 186
AseI ATTAAT 1 cut(s) 20
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 2 cut(s) 523, 839
AsuHPI GGTGA 2 cut(s) 276, 947
AvaII GGWCC 1 cut(s) 839
BbrPI CACGTG 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 655, 958
BccI CCATC 4 cut(s) 230, 758, 787, 1005
BceAI ACGGC 2 cut(s) 211, 341
BciT130I CCWGG 1 cut(s) 849
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 3 cut(s) 94, 100, 764
BfaI CTAG 3 cut(s) 260, 663, 756
BglII AGATCT 1 cut(s) 1016
BisI GCNGC 4 cut(s) 241, 438, 644, 972
BlsI GCNGC 4 cut(s) 242, 439, 645, 973
Bme1390I CCNGG 1 cut(s) 849
Bme18I GGWCC 1 cut(s) 839
BmgT120I GGNCC 2 cut(s) 523, 839
BmiI GGNNCC 2 cut(s) 846, 869
BmrFI CCNGG 1 cut(s) 849
BmsI GCATC 5 cut(s) 207, 609, 644, 675, 722
Bpu10I CCTNAGC 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 1015
BsaI GGTCTC 1 cut(s) 100
BsaWI WCCGGW 3 cut(s) 149, 221, 841
Bsc4I CCNNNNNNNGG 6 cut(s) 180, 484, 610, 756, 848, 981
Bse118I RCCGGY 3 cut(s) 440, 497, 550
Bse3DI GCAATG 1 cut(s) 389
Bse8I GATNNNNATC 1 cut(s) 1015
BseBI CCWGG 1 cut(s) 849
BseGI GGATG 6 cut(s) 600, 659, 779, 931, 994, 1016
BseJI GATNNNNATC 1 cut(s) 1015
BseLI CCNNNNNNNGG 6 cut(s) 180, 484, 610, 756, 848, 981
BseMI GCAATG 1 cut(s) 389
BseMII CTCAG 4 cut(s) 95, 95, 422, 1041
BseRI GAGGAG 1 cut(s) 463
BseXI GCAGC 2 cut(s) 655, 958
Bsh1236I CGCG 2 cut(s) 243, 812
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 240, 524
BsiEI CGRYCG 1 cut(s) 226
BsiSI CCGG 7 cut(s) 150, 222, 315, 441, 498, 551, 842
BslFI GGGAC 1 cut(s) 998
BslI CCNNNNNNNGG 6 cut(s) 180, 484, 610, 756, 848, 981
BsmAI GTCTC 3 cut(s) 94, 100, 764
BsmFI GGGAC 1 cut(s) 998
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 2 cut(s) 240, 524
Bso31I GGTCTC 1 cut(s) 100
Bsp143I GATC 4 cut(s) 463, 574, 961, 1016
BspACI CCGC 3 cut(s) 241, 438, 584
BspANI GGCC 2 cut(s) 240, 524
BspCNI CTCAG 4 cut(s) 94, 96, 421, 1040
BspFNI CGCG 2 cut(s) 243, 812
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 2 cut(s) 846, 869
BspPI GGATC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 389
BsrFI RCCGGY 3 cut(s) 440, 497, 550
BssAI RCCGGY 3 cut(s) 440, 497, 550
BssMI GATC 4 cut(s) 463, 574, 961, 1016
Bst2UI CCWGG 1 cut(s) 849
Bst4CI ACNGT 4 cut(s) 120, 673, 823, 882
Bst6I CTCTTC 2 cut(s) 41, 92
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 442, 499
BstDEI CTNAG 5 cut(s) 58, 81, 104, 408, 1027
BstF5I GGATG 6 cut(s) 600, 659, 779, 931, 994, 1016
BstFNI CGCG 2 cut(s) 243, 812
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 4 cut(s) 466, 577, 964, 1019
BstMAI GTCTC 3 cut(s) 94, 100, 764
BstMBI GATC 4 cut(s) 463, 574, 961, 1016
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 3 cut(s) 498, 590, 663
BstNI CCWGG 1 cut(s) 849
BstSCI CCNGG 1 cut(s) 847
BstUI CGCG 2 cut(s) 243, 812
BstV1I GCAGC 2 cut(s) 655, 958
BstX2I RGATCY 2 cut(s) 961, 1016
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 2 cut(s) 961, 1016
BsuRI GGCC 2 cut(s) 240, 524
BtgZI GCGATG 1 cut(s) 736
BtsCI GGATG 6 cut(s) 600, 659, 779, 931, 994, 1016
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 442, 499
CciI TCATGA 1 cut(s) 558
CfoI GCGC 1 cut(s) 600
Cfr10I RCCGGY 3 cut(s) 440, 497, 550
Cfr13I GGNCC 2 cut(s) 523, 839
CseI GACGC 2 cut(s) 232, 481
CsiI ACCWGGT 1 cut(s) 847
Csp6I GTAC 3 cut(s) 295, 334, 946
CviAII CATG 3 cut(s) 400, 559, 595
CviJI RGCY 9 cut(s) 240, 328, 524, 646, 749, 760, 778, 971, 1052
CviKI_1 RGCY 9 cut(s) 240, 328, 524, 646, 749, 760, 778, 971, 1052
CviQI GTAC 3 cut(s) 295, 334, 946
DdeI CTNAG 5 cut(s) 58, 81, 104, 408, 1027
DpnI GATC 4 cut(s) 465, 576, 963, 1018
DpnII GATC 4 cut(s) 463, 574, 961, 1016
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 41, 92
EarI CTCTTC 2 cut(s) 41, 92
Eco31I GGTCTC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 839
Eco72I CACGTG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 847
FaeI CATG 3 cut(s) 403, 562, 598
FalI AAGNNNNNCTT 2 cut(s) 959, 991
FaqI GGGAC 1 cut(s) 998
FatI CATG 3 cut(s) 399, 558, 594
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 4 cut(s) 241, 438, 644, 972
FokI GGATG 6 cut(s) 587, 666, 766, 938, 981, 1023
Fsp4HI GCNGC 4 cut(s) 241, 438, 644, 972
FspAI RTGCGCAY 1 cut(s) 599
FspBI CTAG 3 cut(s) 260, 663, 756
FspI TGCGCA 1 cut(s) 599
GlaI GCGC 1 cut(s) 599
GluI GCNGC 4 cut(s) 241, 438, 644, 972
HaeIII GGCC 2 cut(s) 240, 524
HapII CCGG 7 cut(s) 150, 222, 315, 441, 498, 551, 842
HgaI GACGC 2 cut(s) 232, 481
HhaI GCGC 1 cut(s) 600
Hin1II CATG 3 cut(s) 403, 562, 598
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HincII GTYRAC 2 cut(s) 589, 898
HindII GTYRAC 2 cut(s) 589, 898
HinfI GANTC 4 cut(s) 68, 134, 176, 721
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 7 cut(s) 150, 222, 315, 441, 498, 551, 842
HphI GGTGA 2 cut(s) 276, 947
Hpy166II GTNNAC 4 cut(s) 297, 589, 898, 1024
Hpy188I TCNGA 7 cut(s) 73, 175, 235, 411, 463, 653, 999
Hpy188III TCNNGA 4 cut(s) 559, 797, 817, 829
Hpy8I GTNNAC 4 cut(s) 297, 589, 898, 1024
Hpy99I CGWCG 3 cut(s) 248, 497, 794
HpyAV CCTTC 7 cut(s) 228, 298, 468, 645, 925, 985, 1001
HpyCH4III ACNGT 4 cut(s) 120, 673, 823, 882
HpyCH4IV ACGT 3 cut(s) 299, 427, 691
HpyCH4V TGCA 4 cut(s) 143, 643, 696, 742
HpyF10VI GCNNNNNNNGC 3 cut(s) 498, 590, 663
HpyF3I CTNAG 5 cut(s) 58, 81, 104, 408, 1027
HpySE526I ACGT 3 cut(s) 299, 427, 691
Hsp92II CATG 3 cut(s) 403, 562, 598
HspAI GCGC 1 cut(s) 598
KroI GCCGGC 2 cut(s) 440, 497
KroNI GCCGGC 2 cut(s) 442, 499
Ksp22I TGATCA 1 cut(s) 574
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 4 cut(s) 463, 574, 961, 1016
Lsp1109I GCAGC 2 cut(s) 655, 958
LweI GCATC 5 cut(s) 207, 609, 644, 675, 722
MabI ACCWGGT 1 cut(s) 847
MaeI CTAG 3 cut(s) 260, 663, 756
MaeII ACGT 3 cut(s) 299, 427, 691
MaeIII GTNAC 5 cut(s) 6, 89, 282, 546, 905
MalI GATC 4 cut(s) 465, 576, 963, 1018
MboI GATC 4 cut(s) 463, 574, 961, 1016
MboII GAAGA 7 cut(s) 28, 46, 109, 716, 952, 971, 1016
MflI RGATCY 2 cut(s) 961, 1016
MluCI AATT 7 cut(s) 186, 288, 343, 579, 630, 679, 951
MluI ACGCGT 1 cut(s) 810
MmeI TCCRAC 2 cut(s) 448, 712
MnlI CCTC 8 cut(s) 90, 350, 443, 481, 484, 488, 668, 852
MroNI GCCGGC 2 cut(s) 440, 497
MseI TTAA 7 cut(s) 20, 372, 588, 678, 762, 875, 1063
MslI CAYNNNNRTG 2 cut(s) 652, 999
MspI CCGG 7 cut(s) 150, 222, 315, 441, 498, 551, 842
MspR9I CCNGG 1 cut(s) 849
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 1 cut(s) 849
MvnI CGCG 2 cut(s) 243, 812
MwoI GCNNNNNNNGC 3 cut(s) 498, 590, 663
NaeI GCCGGC 2 cut(s) 442, 499
NdeII GATC 4 cut(s) 463, 574, 961, 1016
NgoMIV GCCGGC 2 cut(s) 440, 497
NlaIII CATG 3 cut(s) 403, 562, 598
NlaIV GGNNCC 2 cut(s) 846, 869
NmuCI GTSAC 1 cut(s) 282
NsbI TGCGCA 1 cut(s) 599
PagI TCATGA 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 554
PdiI GCCGGC 2 cut(s) 442, 499
PfeI GAWTC 4 cut(s) 68, 134, 176, 721
PflMI CCANNNNNTGG 1 cut(s) 610
PkrI GCNGC 4 cut(s) 242, 439, 645, 973
PmaCI CACGTG 1 cut(s) 300
PmlI CACGTG 1 cut(s) 300
Ppu21I YACGTR 1 cut(s) 300
PshBI ATTAAT 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 691
Psp6I CCWGG 1 cut(s) 847
PspCI CACGTG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 847
PspN4I GGNNCC 2 cut(s) 846, 869
PspPI GGNCC 2 cut(s) 523, 839
PsuI RGATCY 2 cut(s) 961, 1016
RsaI GTAC 3 cut(s) 296, 335, 947
RsaNI GTAC 3 cut(s) 295, 334, 946
RseI CAYNNNNRTG 2 cut(s) 652, 999
SaqAI TTAA 7 cut(s) 20, 372, 588, 678, 762, 875, 1063
SatI GCNGC 4 cut(s) 241, 438, 644, 972
Sau3AI GATC 4 cut(s) 463, 574, 961, 1016
Sau96I GGNCC 2 cut(s) 523, 839
ScrFI CCNGG 1 cut(s) 849
SetI ASST 8 cut(s) 64, 220, 302, 430, 694, 850, 863, 1054
SexAI ACCWGGT 1 cut(s) 847
SfaNI GCATC 5 cut(s) 207, 609, 644, 675, 722
SinI GGWCC 1 cut(s) 839
SmiMI CAYNNNNRTG 2 cut(s) 652, 999
Sse9I AATT 7 cut(s) 186, 288, 343, 579, 630, 679, 951
SsiI CCGC 3 cut(s) 241, 438, 584
SspMI CTAG 3 cut(s) 260, 663, 756
StyD4I CCNGG 1 cut(s) 847
TaaI ACNGT 4 cut(s) 120, 673, 823, 882
TaiI ACGT 3 cut(s) 302, 430, 694
TaqI TCGA 1 cut(s) 857
TaqII GACCGA 2 cut(s) 124, 557
TasI AATT 7 cut(s) 186, 288, 343, 579, 630, 679, 951
TatI WGTACW 1 cut(s) 945
TauI GCSGC 2 cut(s) 243, 440
TfiI GAWTC 4 cut(s) 68, 134, 176, 721
Tru1I TTAA 7 cut(s) 20, 372, 588, 678, 762, 875, 1063
Tru9I TTAA 7 cut(s) 20, 372, 588, 678, 762, 875, 1063
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 282
TseI GCWGC 2 cut(s) 643, 971
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 5 cut(s) 356, 547, 575, 672, 983
TspGWI ACGGA 2 cut(s) 282, 351
TspRI CASTG 1 cut(s) 543
Van91I CCANNNNNTGG 1 cut(s) 610
VpaK11BI GGWCC 1 cut(s) 839
VspI ATTAAT 1 cut(s) 20
XapI RAATTY 1 cut(s) 186
XspI CTAG 3 cut(s) 260, 663, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.