Prupe.2G081000_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001140
Physical Location & Seq
Forward (+)
12508370 .. 12513198
4829 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G081000.1

Sequence Viewer

Length: 903 bp
ATGAAAAACTATGAACTTCAGGTTCATATCAATATATATTCGAAACTTCAGACACCCAAAAATCTCCGCTTTCAAATGGAGAAGCAAACCACCATCGCCTCTTCTTGTTTCTTTCTTTTATACATCGCCACTTTTACAGTGGCAGAAGTTCTTGAAGCTCATCCTCACCTTCATCATCAGCACAACAGTCATAGCTCTGTGAAACTTTTCGTCTTCGGGAATTCGTATGTTGATACTGGGAATATCAAAAAGTCTGTGTCTCCTTCATGGAAGGAACCTTATGAGATCACTTTTCCGAGAAAGCCGGCTGGCCAGTTTTCGAATGGTCGTGTCCTCACTGATTATATAGGTGTTTTCGATACATTGGTCAGTGGACCAAACTTGACCACCCAAATCGACTTTTTCGAACAACTACTTCAACAAAAAGTCTATGCCAAAAACGACGTCGTTAAATCGTCCATTGCTCTCGTATTAGTTGCGGGGAACGACTACGCTGCTCATTTTGGTAGACCAGGCAACGACACAAAGGATTTGGCAATGTTTACCAAATTTATTATTATTAAGCAGCTTACTGTGGATTTGAAGCGTATACATGATTTGGGAGTGCAAAAAATAGCGGTTACAACAATTGAGCCCCTTGGATGTCTTCCCAGCATAACTTCCTCTCTTTCGTATCAAAACTGCAATGAATTTGCCAATATGGCTTCAATGTACCACAACCAGATTCTGCTTCAAAAGTTGGAAGAGTTGAACAAGGAGACAAAGAATTCTGCATTTATCGACTTGGAACTCTATAATGCGTTTATGTCTGCAGTAAAGCCCCCAAAACATCATCAAGGTTCGACCCATTCGAGACGCAAGTCTCTTAATTACAATCCCTTGCTTCAAGGGAACACCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

301

Amino Acids

33.97

Weight (kDa)

9.04

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 447
AccI GTMKAC 2 cut(s) 508, 589
AciI CCGC 3 cut(s) 67, 479, 617
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 4 cut(s) 220, 548, 689, 766
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 1 cut(s) 713
AgsI TTSAA 8 cut(s) 74, 155, 419, 583, 708, 734, 751, 887
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 3 cut(s) 158, 195, 568
AluI AGCT 3 cut(s) 158, 195, 568
Alw26I GTCTC 4 cut(s) 264, 752, 847, 867
AlwNI CAGNNNCTG 1 cut(s) 727
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 494, 565
ApoI RAATTY 4 cut(s) 220, 548, 689, 766
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 158
AsuII TTCGAA 3 cut(s) 41, 320, 405
AvaII GGWCC 1 cut(s) 374
BaeI ACNNNNGTAYC 2 cut(s) 351, 384
BalI TGGCCA 1 cut(s) 312
BanII GRGCYC 1 cut(s) 636
BbsI GAAGAC 2 cut(s) 205, 638
BbvI GCAGC 2 cut(s) 481, 577
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 1 cut(s) 513
BcoDI GTCTC 4 cut(s) 264, 752, 847, 867
BfmI CTRYAG 1 cut(s) 810
BglI GCCNNNNNGGC 1 cut(s) 701
BisI GCNGC 2 cut(s) 495, 566
BlsI GCNGC 2 cut(s) 496, 567
Bme1390I CCNGG 1 cut(s) 513
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 276
BmrFI CCNGG 1 cut(s) 513
BmrI ACTGGG 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 246
BoxI GACNNNNGTC 1 cut(s) 859
BpiI GAAGAC 2 cut(s) 205, 638
Bpu14I TTCGAA 3 cut(s) 41, 320, 405
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 637
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 241, 313
Bse3DI GCAATG 3 cut(s) 459, 543, 691
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 1 cut(s) 637
BseGI GGATG 2 cut(s) 160, 647
BseMI GCAATG 3 cut(s) 459, 543, 691
BseNI ACTGG 2 cut(s) 241, 313
BseXI GCAGC 2 cut(s) 481, 577
BseYI CCCAGC 1 cut(s) 650
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 305
BsmAI GTCTC 4 cut(s) 264, 752, 847, 867
BsmBI CGTCTC 1 cut(s) 847
BsnI GGCC 1 cut(s) 312
Bsp119I TTCGAA 3 cut(s) 41, 320, 405
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 1 cut(s) 285
BspACI CCGC 3 cut(s) 67, 479, 617
BspANI GGCC 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 276
BspMAI CTGCAG 1 cut(s) 814
BspT104I TTCGAA 3 cut(s) 41, 320, 405
BsrDI GCAATG 3 cut(s) 459, 543, 691
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 241, 313
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 637
BssMI GATC 1 cut(s) 285
BssNAI GTATAC 1 cut(s) 590
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 1 cut(s) 637
Bst1107I GTATAC 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 139, 188, 574
Bst6I CTCTTC 2 cut(s) 106, 738
BstACI GRCGYC 1 cut(s) 444
BstBI TTCGAA 3 cut(s) 41, 320, 405
BstC8I GCNNGC 2 cut(s) 306, 310
BstF5I GGATG 2 cut(s) 160, 647
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 4 cut(s) 264, 752, 847, 867
BstMBI GATC 1 cut(s) 285
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstNI CCWGG 1 cut(s) 513
BstPAI GACNNNNGTC 1 cut(s) 859
BstSCI CCNGG 1 cut(s) 511
BstSFI CTRYAG 1 cut(s) 810
BstV1I GCAGC 2 cut(s) 481, 577
BstV2I GAAGAC 2 cut(s) 205, 638
BstZ17I GTATAC 1 cut(s) 590
BsuRI GGCC 1 cut(s) 312
BtgZI GCGATG 2 cut(s) 79, 109
BtsCI GGATG 2 cut(s) 160, 647
BtsIMutI CAGTG 3 cut(s) 144, 336, 376
Cac8I GCNNGC 2 cut(s) 306, 310
CaiI CAGNNNCTG 1 cut(s) 727
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 374
CseI GACGC 1 cut(s) 864
Csp6I GTAC 1 cut(s) 712
CviAII CATG 2 cut(s) 267, 593
CviJI RGCY 9 cut(s) 158, 195, 304, 308, 312, 568, 634, 704, 820
CviKI_1 RGCY 9 cut(s) 158, 195, 304, 308, 312, 568, 634, 704, 820
CviQI GTAC 1 cut(s) 712
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 106, 738
EarI CTCTTC 2 cut(s) 106, 738
Eco130I CCWWGG 1 cut(s) 637
Eco24I GRGCYC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 32
EcoRI GAATTC 2 cut(s) 220, 766
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 637
Esp3I CGTCTC 1 cut(s) 847
FaeI CATG 2 cut(s) 270, 596
FatI CATG 2 cut(s) 266, 592
FauI CCCGC 1 cut(s) 472
FblI GTMKAC 2 cut(s) 508, 589
Fnu4HI GCNGC 2 cut(s) 495, 566
FokI GGATG 2 cut(s) 147, 654
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 2 cut(s) 495, 566
GluI GCNGC 2 cut(s) 495, 566
GsaI CCCAGC 1 cut(s) 654
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 305
HgaI GACGC 1 cut(s) 864
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 2 cut(s) 270, 596
HinfI GANTC 1 cut(s) 724
HpaII CCGG 1 cut(s) 305
HphI GGTGA 1 cut(s) 158
Hpy166II GTNNAC 4 cut(s) 374, 509, 543, 590
Hpy188I TCNGA 2 cut(s) 51, 297
Hpy188III TCNNGA 3 cut(s) 152, 217, 852
Hpy8I GTNNAC 4 cut(s) 374, 509, 543, 590
Hpy99I CGWCG 2 cut(s) 446, 449
HpyAV CCTTC 3 cut(s) 179, 265, 273
HpyCH4III ACNGT 3 cut(s) 139, 188, 574
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 4 cut(s) 607, 684, 773, 812
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 2 cut(s) 270, 596
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Kzo9I GATC 1 cut(s) 285
LpnPI CCDG 9 cut(s) 5, 222, 294, 318, 326, 498, 525, 664, 734
Lsp1109I GCAGC 2 cut(s) 481, 577
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 619
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MboII GAAGA 4 cut(s) 93, 205, 638, 755
MfeI CAATTG 1 cut(s) 627
MhlI GDGCHC 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 6 cut(s) 220, 548, 627, 689, 766, 868
MluNI TGGCCA 1 cut(s) 312
MmeI TCCRAC 1 cut(s) 720
MnlI CCTC 4 cut(s) 109, 174, 344, 673
Mox20I TGGCCA 1 cut(s) 312
MroNI GCCGGC 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 312
MseI TTAA 3 cut(s) 450, 561, 867
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 513
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 701
NaeI GCCGGC 1 cut(s) 306
NdeII GATC 1 cut(s) 285
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 2 cut(s) 270, 596
NlaIV GGNNCC 1 cut(s) 276
NspV TTCGAA 3 cut(s) 41, 320, 405
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 848
PdiI GCCGGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 724
PkrI GCNGC 2 cut(s) 496, 567
PshAI GACNNNNGTC 1 cut(s) 859
Psp6I CCWGG 1 cut(s) 511
PspFI CCCAGC 1 cut(s) 650
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 374
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 727
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
SaqAI TTAA 3 cut(s) 450, 561, 867
SatI GCNGC 2 cut(s) 495, 566
Sau3AI GATC 1 cut(s) 285
Sau96I GGNCC 1 cut(s) 374
ScrFI CCNGG 1 cut(s) 513
SduI GDGCHC 1 cut(s) 636
SetI ASST 9 cut(s) 24, 160, 171, 197, 280, 352, 447, 570, 841
SfcI CTRYAG 1 cut(s) 810
SfuI TTCGAA 3 cut(s) 41, 320, 405
SinI GGWCC 1 cut(s) 374
Sse9I AATT 6 cut(s) 220, 548, 627, 689, 766, 868
SsiI CCGC 3 cut(s) 67, 479, 617
StyD4I CCNGG 1 cut(s) 511
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 3 cut(s) 139, 188, 574
TaiI ACGT 1 cut(s) 447
TaqI TCGA 8 cut(s) 41, 320, 357, 396, 405, 780, 842, 851
TasI AATT 6 cut(s) 220, 548, 627, 689, 766, 868
TfiI GAWTC 1 cut(s) 724
Tru1I TTAA 3 cut(s) 450, 561, 867
Tru9I TTAA 3 cut(s) 450, 561, 867
TscAI CASTG 3 cut(s) 144, 343, 376
TseI GCWGC 2 cut(s) 494, 565
TspDTI ATGAA 6 cut(s) 14, 17, 27, 161, 255, 702
TspRI CASTG 3 cut(s) 144, 343, 376
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 4 cut(s) 220, 548, 689, 766
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 320
XmiI GTMKAC 2 cut(s) 508, 589
XmnI GAANNNNTTC 1 cut(s) 206
ZraI GACGTC 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.