Rroxscaffold_6G00408630

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001140
Physical Location & Seq
Forward (+)
31131553 .. 31136391
4839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00408630.1

Sequence Viewer

Length: 1086 bp
ATGGAATTAGCTCAGACCCTCTTCCTCCCTCTCGTTCTGTGTCTTCTCTCTCTTTTAACACAACAGAGTGCGCTACTGGTCTCAGCTGGTCACCACCATGGTCACCATGGCCACCACCACCACCACCATCATACACACCGATACTTGTTCGATTGGCGTCCAACGAAACTGTTTGTTTTCGGAGACTCGTACTCCGACACAGGCAACAACATAAAGTCTGTGACCAAATCTTGGAAATATCCTTACGGAATCACCTTCCCGGGAAAACCCACCGGCCGTTTCTCCGATGGCCGCGTCCTCACTGATTTCCTTGCTAAGTTTATAGGGGTGAAGTCTCCAATACCATACAAATTTAGAAATGTCGGAGTTAAACATTTGAAATACGGAATGAATTTCGCATATGGAGGCACCGGTGTGTTCAATACCTTGGTTGCGGCACCAAACATGACCACCCAAATCGATTTTTTTCATCAATTCATCAAAGACTCCGTTTTTAATGCGCAAGACCTACACTCCTCCGTGGCATTGATCACTCTTGCAGGCAATGACTACGCATATTACCTGGCCCTGAATAATTCTTCTGAGGGTTTTAAACCTTTCATCACATCAGTTGTAAATCAACTAAGTGTGAACTTGAAACGTGTCTATGACTTGGGAGTGAAGAAAATAGTTGTGACAGCTCTACAACCTTTGGGATGTCTTCCTGGTAGCACAGCCGAATTCTCATTCCAGAAGTGCAATGGAACACAAAACTTGCTGGTCGGTTTCCACAACCTCTTGTTGCAACAAGCTATTGCCAAGTTGAACAATGAATCCAAGAGTTCTTTCATTGTTCTCGATCTATATACCTCATTCATGTCCGTGCTGAAGAATAAAGGAGACCATCTAGGAAGCATAAAGTTTGAGAACCCGTTGAGGCCATGTTGTGTTGGCGATTGTGGGAGTGTCGATGAGAGTGGGGCGAAGAAGTACACCATATGCGACAACCCGAAATCTGCATTCTTTTGGGACGGTGCTCACCCTACCCAACAGGGCTGGCTTGCTGTGTATTCAGCTTTGCAAGGCACACTTGAAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

361

Amino Acids

40.13

Weight (kDa)

8.91

Isoelectric Point (pI)

22.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 57 - 350 1.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 501
AccB1I GGYRCC 2 cut(s) 407, 436
AccB7I CCANNNNNTGG 1 cut(s) 231
AccII CGCG 1 cut(s) 294
AciI CCGC 2 cut(s) 292, 434
AcoI YGGCCR 3 cut(s) 109, 274, 289
AcsI RAATTY 3 cut(s) 350, 391, 719
AcuI CTGAAG 1 cut(s) 887
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 3 cut(s) 191, 971, 1082
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 640
AgeI ACCGGT 1 cut(s) 410
AgsI TTSAA 5 cut(s) 379, 421, 637, 805, 1073
AjnI CCWGG 2 cut(s) 561, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AleI CACNNNNGTG 1 cut(s) 413
AloI GAACNNNNNNTCC 2 cut(s) 797, 829
AluBI AGCT 5 cut(s) 11, 86, 680, 791, 1055
AluI AGCT 5 cut(s) 11, 86, 680, 791, 1055
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 4 cut(s) 85, 177, 339, 873
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 5 cut(s) 109, 274, 289, 564, 917
ApoI RAATTY 3 cut(s) 350, 391, 719
AsiGI ACCGGT 1 cut(s) 410
AspLEI GCGC 2 cut(s) 73, 502
AspS9I GGNCC 1 cut(s) 565
AsuC2I CCSGG 2 cut(s) 260, 261
AsuHPI GGTGA 5 cut(s) 83, 95, 244, 340, 1010
AvaI CYCGRG 1 cut(s) 259
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 2 cut(s) 407, 436
BbsI GAAGAC 2 cut(s) 35, 692
Bbv12I GWGCWC 1 cut(s) 1018
BccI CCATC 3 cut(s) 135, 281, 891
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 563, 705
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 2 cut(s) 260, 261
BcoDI GTCTC 4 cut(s) 85, 177, 339, 873
BfaI CTAG 2 cut(s) 887, 1084
BisI GCNGC 2 cut(s) 292, 435
BlsI GCNGC 2 cut(s) 293, 436
Bme1390I CCNGG 4 cut(s) 260, 261, 563, 705
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 2 cut(s) 409, 438
BmrFI CCNGG 4 cut(s) 260, 261, 563, 705
BpiI GAAGAC 2 cut(s) 35, 692
BpuMI CCSGG 2 cut(s) 260, 261
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 157
BsaI GGTCTC 2 cut(s) 85, 873
BsaJI CCNNGG 5 cut(s) 97, 106, 259, 426, 519
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 497, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 2 cut(s) 272, 410
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 2 cut(s) 550, 745
BseBI CCWGG 2 cut(s) 563, 705
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 5 cut(s) 97, 106, 259, 426, 519
BseGI GGATG 1 cut(s) 701
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 2 cut(s) 550, 745
BseMII CTCAG 3 cut(s) 26, 96, 573
BseNI ACTGG 1 cut(s) 81
BseRI GAGGAG 1 cut(s) 505
BseX3I CGGCCG 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 5 cut(s) 111, 276, 291, 566, 919
BshNI GGYRCC 2 cut(s) 407, 436
BshTI ACCGGT 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 3 cut(s) 260, 273, 411
BslFI GGGAC 1 cut(s) 1022
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 4 cut(s) 85, 177, 339, 873
BsmFI GGGAC 1 cut(s) 1022
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 5 cut(s) 111, 276, 291, 566, 919
Bso31I GGTCTC 2 cut(s) 85, 873
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp143I GATC 2 cut(s) 528, 838
Bsp19I CCATGG 2 cut(s) 97, 106
BspACI CCGC 2 cut(s) 292, 434
BspANI GGCC 5 cut(s) 111, 276, 291, 566, 919
BspCNI CTCAG 3 cut(s) 25, 95, 574
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 2 cut(s) 409, 438
BspT107I GGYRCC 2 cut(s) 407, 436
BspTNI GGTCTC 2 cut(s) 85, 873
BsrDI GCAATG 2 cut(s) 550, 745
BsrFI RCCGGY 2 cut(s) 272, 410
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 272, 410
BssECI CCNNGG 5 cut(s) 97, 106, 259, 426, 519
BssMI GATC 2 cut(s) 528, 838
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 3 cut(s) 97, 106, 426
Bst2UI CCWGG 2 cut(s) 563, 705
Bst4CI ACNGT 2 cut(s) 171, 1013
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 541, 1037, 1041
BstDEI CTNAG 5 cut(s) 12, 82, 315, 582, 623
BstDSI CCRYGG 3 cut(s) 97, 106, 519
BstEII GGTNACC 2 cut(s) 89, 101
BstF5I GGATG 1 cut(s) 701
BstFNI CGCG 1 cut(s) 294
BstHHI GCGC 2 cut(s) 73, 502
BstKTI GATC 2 cut(s) 531, 841
BstMAI GTCTC 4 cut(s) 85, 177, 339, 873
BstMBI GATC 2 cut(s) 528, 838
BstMCI CGRYCG 1 cut(s) 277
BstNI CCWGG 2 cut(s) 563, 705
BstPI GGTNACC 2 cut(s) 89, 101
BstSCI CCNGG 4 cut(s) 258, 259, 561, 703
BstUI CGCG 1 cut(s) 294
BstV2I GAAGAC 2 cut(s) 35, 692
BstZI CGGCCG 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 5 cut(s) 111, 276, 291, 566, 919
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 3 cut(s) 97, 106, 519
BtsCI GGATG 1 cut(s) 701
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 3 cut(s) 541, 1037, 1041
CfoI GCGC 2 cut(s) 73, 502
Cfr10I RCCGGY 2 cut(s) 272, 410
Cfr13I GGNCC 1 cut(s) 565
Cfr9I CCCGGG 1 cut(s) 259
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 2 cut(s) 146, 283
Csp6I GTAC 3 cut(s) 190, 970, 1081
CspAI ACCGGT 1 cut(s) 410
CviAII CATG 5 cut(s) 98, 107, 445, 856, 921
CviQI GTAC 3 cut(s) 190, 970, 1081
DdeI CTNAG 5 cut(s) 12, 82, 315, 582, 623
DpnI GATC 2 cut(s) 530, 840
DpnII GATC 2 cut(s) 528, 838
DraI TTTAAA 1 cut(s) 592
EaeI YGGCCR 3 cut(s) 109, 274, 289
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 3 cut(s) 97, 106, 426
Eco31I GGTCTC 2 cut(s) 85, 873
Eco52I CGGCCG 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 259
Eco91I GGTNACC 2 cut(s) 89, 101
EcoO65I GGTNACC 2 cut(s) 89, 101
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 2 cut(s) 561, 703
EcoT14I CCWWGG 3 cut(s) 97, 106, 426
ErhI CCWWGG 3 cut(s) 97, 106, 426
FaeI CATG 5 cut(s) 101, 110, 448, 859, 924
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 1 cut(s) 1022
FatI CATG 5 cut(s) 97, 106, 444, 855, 920
FauNDI CATATG 2 cut(s) 400, 977
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 2 cut(s) 292, 435
FokI GGATG 1 cut(s) 708
Fsp4HI GCNGC 2 cut(s) 292, 435
FspBI CTAG 2 cut(s) 887, 1084
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 2 cut(s) 72, 501
GluI GCNGC 2 cut(s) 292, 435
HaeIII GGCC 5 cut(s) 111, 276, 291, 566, 919
HapII CCGG 3 cut(s) 260, 273, 411
HgaI GACGC 2 cut(s) 146, 283
HhaI GCGC 2 cut(s) 73, 502
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 101, 110, 448, 859, 924
Hin6I GCGC 2 cut(s) 71, 500
HinP1I GCGC 2 cut(s) 71, 500
HinfI GANTC 4 cut(s) 185, 249, 485, 812
HpaII CCGG 3 cut(s) 260, 273, 411
HphI GGTGA 5 cut(s) 83, 95, 244, 340, 1010
Hpy166II GTNNAC 2 cut(s) 631, 972
Hpy188I TCNGA 6 cut(s) 15, 182, 196, 286, 365, 583
Hpy188III TCNNGA 2 cut(s) 730, 836
Hpy8I GTNNAC 2 cut(s) 631, 972
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 171, 1013
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 5 cut(s) 539, 738, 784, 998, 1060
HpyF3I CTNAG 5 cut(s) 12, 82, 315, 582, 623
HpySE526I ACGT 1 cut(s) 640
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 101, 110, 448, 859, 924
HspAI GCGC 2 cut(s) 71, 500
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 528, 838
MaeI CTAG 2 cut(s) 887, 1084
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 4 cut(s) 89, 101, 220, 673
MalI GATC 2 cut(s) 530, 840
MboI GATC 2 cut(s) 528, 838
MboII GAAGA 8 cut(s) 13, 35, 570, 673, 692, 880, 976, 1085
MhlI GDGCHC 1 cut(s) 1018
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 7 cut(s) 5, 350, 391, 473, 574, 719, 1076
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 2 cut(s) 179, 479
MmeI TCCRAC 3 cut(s) 185, 219, 343
Mox20I TGGCCA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 111
MseI TTAA 4 cut(s) 56, 369, 495, 591
MslI CAYNNNNRTG 3 cut(s) 96, 413, 860
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 3 cut(s) 260, 273, 411
MspR9I CCNGG 4 cut(s) 260, 261, 563, 705
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 2 cut(s) 563, 705
MvnI CGCG 1 cut(s) 294
NciI CCSGG 2 cut(s) 260, 261
NcoI CCATGG 2 cut(s) 97, 106
NdeI CATATG 2 cut(s) 400, 977
NdeII GATC 2 cut(s) 528, 838
NlaIII CATG 5 cut(s) 101, 110, 448, 859, 924
NlaIV GGNNCC 2 cut(s) 409, 438
NmuCI GTSAC 4 cut(s) 89, 101, 220, 673
NsbI TGCGCA 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 413
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 2 cut(s) 249, 812
PflMI CCANNNNNTGG 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 410
PkrI GCNGC 2 cut(s) 293, 436
PleI GAGTC 2 cut(s) 179, 479
PpsI GAGTC 2 cut(s) 179, 479
Psp6I CCWGG 2 cut(s) 561, 703
PspEI GGTNACC 2 cut(s) 89, 101
PspGI CCWGG 2 cut(s) 561, 703
PspN4I GGNNCC 2 cut(s) 409, 438
PspPI GGNCC 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 3 cut(s) 191, 971, 1082
RsaNI GTAC 3 cut(s) 190, 970, 1081
RseI CAYNNNNRTG 3 cut(s) 96, 413, 860
SaqAI TTAA 4 cut(s) 56, 369, 495, 591
SatI GCNGC 2 cut(s) 292, 435
Sau3AI GATC 2 cut(s) 528, 838
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 2 cut(s) 179, 479
ScrFI CCNGG 4 cut(s) 260, 261, 563, 705
SduI GDGCHC 1 cut(s) 1018
SgrAI CRCCGGYG 1 cut(s) 410
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 3 cut(s) 96, 413, 860
Sse9I AATT 7 cut(s) 5, 350, 391, 473, 574, 719, 1076
SsiI CCGC 2 cut(s) 292, 434
SspMI CTAG 2 cut(s) 887, 1084
StyD4I CCNGG 4 cut(s) 258, 259, 561, 703
StyI CCWWGG 3 cut(s) 97, 106, 426
TaaI ACNGT 2 cut(s) 171, 1013
TaiI ACGT 1 cut(s) 643
TaqI TCGA 4 cut(s) 150, 459, 837, 948
TasI AATT 7 cut(s) 5, 350, 391, 473, 574, 719, 1076
TatI WGTACW 2 cut(s) 969, 1080
TauI GCSGC 2 cut(s) 294, 437
TfiI GAWTC 2 cut(s) 249, 812
Tru1I TTAA 4 cut(s) 56, 369, 495, 591
Tru9I TTAA 4 cut(s) 56, 369, 495, 591
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 4 cut(s) 89, 101, 220, 673
Tsp45I GTSAC 4 cut(s) 89, 101, 220, 673
TspDTI ATGAA 7 cut(s) 404, 458, 466, 589, 817, 825, 844
TspGWI ACGGA 5 cut(s) 261, 399, 478, 508, 850
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 231
XapI RAATTY 3 cut(s) 350, 391, 719
XcmI CCANNNNNNNNNTGG 2 cut(s) 104, 737
XmaI CCCGGG 1 cut(s) 259
XspI CTAG 2 cut(s) 887, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.