RLG00000029642

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001140
Physical Location & Seq
Reverse (-)
41946744 .. 41950236
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029642

Sequence Viewer

Length: 1098 bp
ATGGAGCAAACGACCATCGTCTCATTCTGTCTCTTTCTTCTCTACAGTTCCACTTGTCCACAAGTACTTCATTCAGCCATAACCTCCAACCACCGCCACCGCCATCGCCACCAACACCAACACCATGACAGGAGGTCTGTAAAGCTTTTTGTTTTCGGGGATTCGTATGCTGATACTGGGAATAGCGGCAAATTTGCGACTGGTTCATGGAAGCCGCCTTATGGGATCACTTTTCCTGGAAAGCCGGCAGGCCGATTCTCCGATGGTCGCGTGCTCACTGATTATATAGCTTCCTTTCTGGGTATAAGATCTCCAGTACCTTACGAATTGAGAAACACTGAGAAGAAATCGAAATTAAGGAACGGGATGAACTTTGCATTTGGAGGAACTGGTGTTTTTGAGACTTTGGTCAGTGCACCAAACATGACTACCCAAATCGACTTTCTCCAACAACTAGTGGAAGAAAAATTGTATACCAGAAGTGACTTGGGCTCGTCCGTCGCTTTAGTATCACTAGCTGGAAACGACTATGCTGCTTATCTTCTTAAAGGAAACCAGAGTCAGGATTTGCCAGCGGTGTCCACATCGATTGTTAACCAGCTAGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGCAAAATATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGCAACTTCCATTTTTTCTTCTTATCAGAATTGCAGTGAAAGTATGAATGCAGGTTCAAGATTCCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTAAAGAAGCAGACAACTACTAGATCTACATTTGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATTCAACAACAAAAACACCTGCAACAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAGAAGTGGAGCAAATAAGTACAAAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACAAGGAGCACCTTTCGCAGAATGGCTGGCATACTGTTTATTCTGCAATCAAGTCTTCCCTTCGTCCATTGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

366

Amino Acids

40.67

Weight (kDa)

9.13

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 49 - 356 9.1e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 879
Acc16I TGCGCA 1 cut(s) 641
Acc36I ACCTGC 2 cut(s) 728, 879
AccB7I CCANNNNNTGG 1 cut(s) 604
AccI GTMKAC 1 cut(s) 473
AccII CGCG 1 cut(s) 270
AccIII TCCGGA 1 cut(s) 1000
AciI CCGC 7 cut(s) 94, 100, 186, 215, 575, 687, 849
AclWI GGATC 2 cut(s) 233, 834
AcsI RAATTY 2 cut(s) 191, 883
AfaI GTAC 3 cut(s) 66, 318, 983
AfiI CCNNNNNNNGG 3 cut(s) 221, 562, 604
AgsI TTSAA 3 cut(s) 744, 779, 857
AhdI GACNNNNNGTC 1 cut(s) 133
AhlI ACTAGT 1 cut(s) 454
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 4 cut(s) 145, 290, 518, 601
AluI AGCT 4 cut(s) 145, 290, 518, 601
Alw21I GWGCWC 3 cut(s) 276, 418, 1032
Alw26I GTCTC 3 cut(s) 25, 35, 395
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 2 cut(s) 233, 834
Aor13HI TCCGGA 1 cut(s) 1000
AoxI GGCC 1 cut(s) 250
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 533, 933
ApoI RAATTY 2 cut(s) 191, 883
AspLEI GCGC 1 cut(s) 642
AsuHPI GGTGA 2 cut(s) 619, 940
BaeGI GKGCMC 1 cut(s) 418
BanII GRGCYC 2 cut(s) 494, 669
BauI CACGAG 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 1068
Bbv12I GWGCWC 3 cut(s) 276, 418, 1032
BbvI GCAGC 2 cut(s) 520, 945
BccI CCATC 3 cut(s) 23, 111, 257
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 1 cut(s) 237
BcoDI GTCTC 3 cut(s) 25, 35, 395
BcuI ACTAGT 1 cut(s) 454
BfaI CTAG 4 cut(s) 455, 515, 602, 804
BfmI CTRYAG 1 cut(s) 43
BfuAI ACCTGC 2 cut(s) 728, 879
BglII AGATCT 2 cut(s) 308, 806
BisI GCNGC 5 cut(s) 187, 215, 534, 687, 934
BlsI GCNGC 5 cut(s) 188, 216, 535, 688, 935
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 237
BmeRI GACNNNNNGTC 1 cut(s) 133
BmrFI CCNGG 1 cut(s) 237
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 900
BmuI ACTGGG 1 cut(s) 186
BoxI GACNNNNGTC 2 cut(s) 17, 407
BpiI GAAGAC 1 cut(s) 1068
BpmI CTGGAG 1 cut(s) 297
Bsa29I ATCGAT 1 cut(s) 587
BsaJI CCNNGG 1 cut(s) 669
BsaWI WCCGGW 1 cut(s) 1000
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 562, 604
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 4 cut(s) 181, 205, 314, 394
BseAI TCCGGA 1 cut(s) 1000
BseBI CCWGG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 2 cut(s) 372, 680
BseLI CCNNNNNNNGG 3 cut(s) 221, 562, 604
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 4 cut(s) 181, 205, 314, 394
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 2 cut(s) 520, 945
BsgI GTGCAG 1 cut(s) 1012
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 252
BshVI ATCGAT 1 cut(s) 587
BsiHKAI GWGCWC 3 cut(s) 276, 418, 1032
BsiSI CCGG 2 cut(s) 245, 1001
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 3 cut(s) 221, 562, 604
BsmAI GTCTC 3 cut(s) 25, 35, 395
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 1034
BsmI GAATGC 1 cut(s) 739
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 5 cut(s) 276, 418, 494, 669, 1032
Bsp13I TCCGGA 1 cut(s) 1000
Bsp143I GATC 5 cut(s) 225, 308, 806, 826, 893
Bsp19I CCATGG 1 cut(s) 669
BspACI CCGC 7 cut(s) 94, 100, 186, 215, 575, 687, 849
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 587
BspEI TCCGGA 1 cut(s) 1000
BspFNI CGCG 1 cut(s) 270
BspMI ACCTGC 2 cut(s) 728, 879
BspPI GGATC 2 cut(s) 233, 834
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 4 cut(s) 181, 205, 314, 394
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 5 cut(s) 225, 308, 806, 826, 893
BssNAI GTATAC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 943
BssT1I CCWWGG 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 943
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 47, 1057
BstC8I GCNNGC 6 cut(s) 246, 250, 272, 573, 684, 1049
BstDEI CTNAG 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 2 cut(s) 372, 680
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 642
BstKTI GATC 5 cut(s) 228, 311, 809, 829, 896
BstMAI GTCTC 3 cut(s) 25, 35, 395
BstMBI GATC 5 cut(s) 225, 308, 806, 826, 893
BstMWI GCNNNNNNNGC 1 cut(s) 1036
BstNI CCWGG 1 cut(s) 237
BstPAI GACNNNNGTC 2 cut(s) 17, 407
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 520, 945
BstV2I GAAGAC 1 cut(s) 1068
BstX2I RGATCY 3 cut(s) 308, 806, 826
BstYI RGATCY 3 cut(s) 308, 806, 826
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 587
BsuRI GGCC 1 cut(s) 252
BsuTUI ATCGAT 1 cut(s) 587
BtgI CCRYGG 1 cut(s) 669
BtgZI GCGATG 1 cut(s) 89
BtsCI GGATG 2 cut(s) 372, 680
BtsI GCAGTG 1 cut(s) 727
BtsIMutI CAGTG 5 cut(s) 276, 336, 418, 727, 905
BveI ACCTGC 2 cut(s) 728, 879
Cac8I GCNNGC 6 cut(s) 246, 250, 272, 573, 684, 1049
CfoI GCGC 1 cut(s) 642
Cfr10I RCCGGY 1 cut(s) 244
ClaI ATCGAT 1 cut(s) 587
Csp6I GTAC 3 cut(s) 65, 317, 982
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 5 cut(s) 125, 207, 424, 670, 915
CviQI GTAC 3 cut(s) 65, 317, 982
DdeI CTNAG 1 cut(s) 339
DpnI GATC 5 cut(s) 227, 310, 808, 828, 895
DpnII GATC 5 cut(s) 225, 308, 806, 826, 893
DraI TTTAAA 1 cut(s) 786
DriI GACNNNNNGTC 1 cut(s) 133
Eam1105I GACNNNNNGTC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 669
Eco24I GRGCYC 2 cut(s) 494, 669
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 669
EcoT22I ATGCAT 1 cut(s) 841
EcoT38I GRGCYC 2 cut(s) 494, 669
ErhI CCWWGG 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 5 cut(s) 128, 210, 427, 673, 918
FaqI GGGAC 1 cut(s) 1034
FatI CATG 5 cut(s) 124, 206, 423, 669, 914
FblI GTMKAC 1 cut(s) 473
Fnu4HI GCNGC 5 cut(s) 187, 215, 534, 687, 934
FokI GGATG 2 cut(s) 379, 687
FriOI GRGCYC 2 cut(s) 494, 669
Fsp4HI GCNGC 5 cut(s) 187, 215, 534, 687, 934
FspBI CTAG 4 cut(s) 455, 515, 602, 804
FspI TGCGCA 1 cut(s) 641
GlaI GCGC 1 cut(s) 641
GluI GCNGC 5 cut(s) 187, 215, 534, 687, 934
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 1 cut(s) 252
HapII CCGG 2 cut(s) 245, 1001
HhaI GCGC 1 cut(s) 642
Hin1II CATG 5 cut(s) 128, 210, 427, 673, 918
Hin6I GCGC 1 cut(s) 640
HinP1I GCGC 1 cut(s) 640
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 5 cut(s) 161, 255, 559, 747, 901
HpaI GTTAAC 1 cut(s) 595
HpaII CCGG 2 cut(s) 245, 1001
HphI GGTGA 2 cut(s) 619, 940
Hpy166II GTNNAC 7 cut(s) 59, 416, 474, 582, 595, 610, 958
Hpy188I TCNGA 3 cut(s) 262, 714, 898
Hpy188III TCNNGA 3 cut(s) 563, 744, 1001
Hpy8I GTNNAC 7 cut(s) 59, 416, 474, 582, 595, 610, 958
Hpy99I CGWCG 1 cut(s) 503
HpyAV CCTTC 1 cut(s) 1091
HpyCH4III ACNGT 2 cut(s) 47, 1057
HpyCH4V TGCA 9 cut(s) 377, 416, 720, 737, 766, 839, 874, 993, 1067
HpyF10VI GCNNNNNNNGC 1 cut(s) 1036
HpyF3I CTNAG 1 cut(s) 339
Hsp92II CATG 5 cut(s) 128, 210, 427, 673, 918
HspAI GCGC 1 cut(s) 640
Kpn2I TCCGGA 1 cut(s) 1000
KroI GCCGGC 1 cut(s) 244
KroNI GCCGGC 1 cut(s) 246
KspAI GTTAAC 1 cut(s) 595
Kzo9I GATC 5 cut(s) 225, 308, 806, 826, 893
LmnI GCTCC 3 cut(s) 4, 971, 1027
Lsp1109I GCAGC 2 cut(s) 520, 945
LweI GCATC 1 cut(s) 900
MaeI CTAG 4 cut(s) 455, 515, 602, 804
MaeIII GTNAC 2 cut(s) 482, 652
MalI GATC 5 cut(s) 227, 310, 808, 828, 895
MboI GATC 5 cut(s) 225, 308, 806, 826, 893
MboII GAAGA 7 cut(s) 29, 355, 473, 533, 696, 791, 1068
MflI RGATCY 3 cut(s) 308, 806, 826
MhlI GDGCHC 5 cut(s) 276, 418, 494, 669, 1032
MluCI AATT 7 cut(s) 191, 326, 353, 467, 715, 852, 883
MlyI GAGTC 1 cut(s) 568
MmeI TCCRAC 2 cut(s) 111, 472
MnlI CCTC 3 cut(s) 94, 126, 377
Mph1103I ATGCAT 1 cut(s) 841
MroI TCCGGA 1 cut(s) 1000
MroNI GCCGGC 1 cut(s) 244
MseI TTAA 5 cut(s) 356, 546, 594, 785, 1096
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 2 cut(s) 245, 1001
MspR9I CCNGG 1 cut(s) 237
Mva1269I GAATGC 1 cut(s) 739
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 1036
NaeI GCCGGC 1 cut(s) 246
NcoI CCATGG 1 cut(s) 669
NdeII GATC 5 cut(s) 225, 308, 806, 826, 893
NgoMIV GCCGGC 1 cut(s) 244
NlaIII CATG 5 cut(s) 128, 210, 427, 673, 918
NmuCI GTSAC 1 cut(s) 482
NsbI TGCGCA 1 cut(s) 641
NsiI ATGCAT 1 cut(s) 841
PaqCI CACCTGC 1 cut(s) 879
PctI GAATGC 1 cut(s) 739
PdiI GCCGGC 1 cut(s) 246
PfeI GAWTC 4 cut(s) 161, 255, 747, 901
PflMI CCANNNNNTGG 1 cut(s) 604
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 5 cut(s) 188, 216, 535, 688, 935
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
PshAI GACNNNNGTC 2 cut(s) 17, 407
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PsuI RGATCY 3 cut(s) 308, 806, 826
RsaI GTAC 3 cut(s) 66, 318, 983
RsaNI GTAC 3 cut(s) 65, 317, 982
SaqAI TTAA 5 cut(s) 356, 546, 594, 785, 1096
SatI GCNGC 5 cut(s) 187, 215, 534, 687, 934
Sau3AI GATC 5 cut(s) 225, 308, 806, 826, 893
ScaI AGTACT 1 cut(s) 66
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 5 cut(s) 276, 418, 494, 669, 1032
SfaNI GCATC 1 cut(s) 900
SfcI CTRYAG 1 cut(s) 43
SpeI ACTAGT 1 cut(s) 454
Sse9I AATT 7 cut(s) 191, 326, 353, 467, 715, 852, 883
SsiI CCGC 7 cut(s) 94, 100, 186, 215, 575, 687, 849
SspI AATATT 1 cut(s) 648
SspMI CTAG 4 cut(s) 455, 515, 602, 804
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 2 cut(s) 47, 1057
TaqI TCGA 4 cut(s) 350, 438, 587, 887
TasI AATT 7 cut(s) 191, 326, 353, 467, 715, 852, 883
TatI WGTACW 2 cut(s) 64, 981
TauI GCSGC 3 cut(s) 189, 217, 689
TfiI GAWTC 4 cut(s) 161, 255, 747, 901
Tru1I TTAA 5 cut(s) 356, 546, 594, 785, 1096
Tru9I TTAA 5 cut(s) 356, 546, 594, 785, 1096
TscAI CASTG 5 cut(s) 283, 343, 418, 727, 912
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 533, 933
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 4 cut(s) 59, 195, 383, 746
TspGWI ACGGA 1 cut(s) 487
TspRI CASTG 5 cut(s) 283, 343, 418, 727, 912
Van91I CCANNNNNTGG 1 cut(s) 604
VneI GTGCAC 1 cut(s) 414
XapI RAATTY 2 cut(s) 191, 883
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 473
XspI CTAG 4 cut(s) 455, 515, 602, 804
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.