pycom14g01920

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001140
Physical Location & Seq
Reverse (-)
1482087 .. 1485063
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g01920.2

Sequence Viewer

Length: 1074 bp
ATGAAACCACCTCAAACACTCTTCCTCCTCCTCACTCTCTTTCTCTTCTACCTCCTCTCAGGACAAAAAGGGGTGCTAGTTTCAGCAGCTCACCACCACCACCACACACAGCGCCACCTATTTGCTTGGCGGCCGACGAAGCTATTTGTCTTTGGAGACTCATATTCTGACACAGGAAACAACGGGAAATCAGTGGCCAGTTCTTGGAAACTACCCTATGGAATCACATTCCCGGGAAAACCCAGTGGCCGTTTCTCCGATGGCCGTGTCCTCACCGATTTCCTTGCTAGGTTTATAGGAGTTAAGTCTCCAATACCATACAGATTTAGAAAAGTCGGGGCCAAACATTTGAAGTATGGAGTGAATTTCGCATATGGAGGCACAGGTGTTTTTAATACCTTGGTTTTGGATCCCAACATGACAACCCAGATTGACTTCTTCCAGAAGCTCATAAAAAACGACTATGTCTTCACTCCCAAGGATCTTCACTCTTCAGTCGCCCTCGTCACCGTTGCGGGTAACGATTACAGGACTTATGTCACCACAAATGGCTCTGTTCAGGGTTGGCAACCCTTCATCACATCAGTCGTGAATCAAGTAACTGTGAACTTGAAACGTATTTATGCCTTGGGAGTGAGCAAAATAGTTGTGACAGCACTTCAACCTTTGGGATGTCTTCCTTCGAGCACGGCAGCATTTTCATTCCAACAATGCAATGGAACTGAGAACGCGCTAGTCAGTTTCCACAACCTTTTGTTGCAGCAAGCTGTGGCCAAGTTGAACAACGAAACCAAGAATTCTTTTATCATTCTTGATCTTTCTGCCTCGTTCACGTCGGTGTTCAAGAACAAAGGAGATCTAGGAAGCATAAAGTTTGAGAACCCATTAAGGCCATGCTGCATTGGTATAAGCAATGAATATTCTTGTGGGAGTGTGGACGAAAGTGGTTCAAAGAAGTACACCATTTGTAAAAATCCTGAATCTGCATTCTTTTGGGACACTGCTCACCCTACCCAACAGGGTTGGCGCGCTGTGTATTCAGCTTTGCAAGCCACACTTGAAGAACTCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

358

Amino Acids

39.57

Weight (kDa)

9.42

Isoelectric Point (pI)

18.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 204
AccII CGCG 2 cut(s) 731, 1029
AciI CCGC 2 cut(s) 130, 515
AclWI GGATC 3 cut(s) 404, 417, 489
AcoI YGGCCR 5 cut(s) 131, 195, 247, 262, 771
AcsI RAATTY 2 cut(s) 364, 796
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 1 cut(s) 959
AfiI CCNNNNNNNGG 1 cut(s) 204
AgsI TTSAA 7 cut(s) 352, 613, 662, 781, 844, 951, 1061
AjiI CACGTC 1 cut(s) 834
AleI CACNNNNGTG 1 cut(s) 836
AluBI AGCT 5 cut(s) 89, 142, 448, 767, 1043
AluI AGCT 5 cut(s) 89, 142, 448, 767, 1043
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 2 cut(s) 150, 312
AlwI GGATC 3 cut(s) 404, 417, 489
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 7 cut(s) 131, 195, 247, 262, 339, 771, 890
ApeKI GCWGC 4 cut(s) 86, 692, 760, 897
ApoI RAATTY 2 cut(s) 364, 796
AspLEI GCGC 4 cut(s) 114, 733, 1029, 1031
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 2 cut(s) 233, 234
AsuHPI GGTGA 5 cut(s) 83, 265, 499, 532, 998
AvaI CYCGRG 1 cut(s) 232
BalI TGGCCA 2 cut(s) 197, 773
BamHI GGATCC 1 cut(s) 409
BbsI GAAGAC 2 cut(s) 460, 668
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 4 cut(s) 98, 704, 772, 884
BccI CCATC 1 cut(s) 254
BceAI ACGGC 3 cut(s) 234, 249, 705
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BcnI CCSGG 2 cut(s) 233, 234
BcoDI GTCTC 2 cut(s) 150, 312
BfaI CTAG 4 cut(s) 77, 288, 734, 860
BfoI RGCGCY 1 cut(s) 115
BglII AGATCT 1 cut(s) 856
BisI GCNGC 5 cut(s) 87, 131, 693, 761, 898
BlsI GCNGC 5 cut(s) 88, 132, 694, 762, 899
Bme1390I CCNGG 2 cut(s) 233, 234
BmeT110I CYCGRG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 834
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 340, 411
BmrFI CCNGG 2 cut(s) 233, 234
BmrI ACTGGG 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 237
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 2 cut(s) 460, 668
BpuMI CCSGG 2 cut(s) 233, 234
BsaJI CCNNGG 4 cut(s) 232, 399, 477, 627
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse1I ACTGG 2 cut(s) 198, 243
Bse3DI GCAATG 2 cut(s) 721, 919
BseDI CCNNGG 4 cut(s) 232, 399, 477, 627
BseGI GGATG 1 cut(s) 677
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMI GCAATG 2 cut(s) 721, 919
BseMII CTCAG 2 cut(s) 72, 714
BseNI ACTGG 2 cut(s) 198, 243
BsePI GCGCGC 1 cut(s) 1027
BseRI GAGGAG 3 cut(s) 17, 20, 44
BseX3I CGGCCG 1 cut(s) 131
BseXI GCAGC 4 cut(s) 98, 704, 772, 884
Bsh1236I CGCG 2 cut(s) 731, 1029
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 7 cut(s) 133, 197, 249, 264, 341, 773, 892
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 1010
BslI CCNNNNNNNGG 1 cut(s) 204
BsmAI GTCTC 2 cut(s) 150, 312
BsmFI GGGAC 1 cut(s) 1010
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 7 cut(s) 133, 197, 249, 264, 341, 773, 892
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 689
Bsp143I GATC 4 cut(s) 409, 481, 814, 856
BspACI CCGC 2 cut(s) 130, 515
BspANI GGCC 7 cut(s) 133, 197, 249, 264, 341, 773, 892
BspCNI CTCAG 2 cut(s) 71, 715
BspFNI CGCG 2 cut(s) 731, 1029
BspLI GGNNCC 2 cut(s) 340, 411
BspPI GGATC 3 cut(s) 404, 417, 489
BsrDI GCAATG 2 cut(s) 721, 919
BsrI ACTGG 2 cut(s) 198, 243
BssECI CCNNGG 4 cut(s) 232, 399, 477, 627
BssHII GCGCGC 1 cut(s) 1027
BssMI GATC 4 cut(s) 409, 481, 814, 856
BssT1I CCWWGG 3 cut(s) 399, 477, 627
Bst4CI ACNGT 2 cut(s) 511, 604
Bst6I CTCTTC 3 cut(s) 26, 50, 496
BstC8I GCNNGC 3 cut(s) 765, 1029, 1050
BstDEI CTNAG 2 cut(s) 58, 723
BstF5I GGATG 1 cut(s) 677
BstFNI CGCG 2 cut(s) 731, 1029
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 4 cut(s) 114, 733, 1029, 1031
BstKTI GATC 4 cut(s) 412, 484, 817, 859
BstMAI GTCTC 2 cut(s) 150, 312
BstMBI GATC 4 cut(s) 409, 481, 814, 856
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 2 cut(s) 139, 1049
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 2 cut(s) 231, 232
BstUI CGCG 2 cut(s) 731, 1029
BstV1I GCAGC 4 cut(s) 98, 704, 772, 884
BstV2I GAAGAC 2 cut(s) 460, 668
BstX2I RGATCY 3 cut(s) 409, 481, 856
BstYI RGATCY 3 cut(s) 409, 481, 856
BstZI CGGCCG 1 cut(s) 131
BsuRI GGCC 7 cut(s) 133, 197, 249, 264, 341, 773, 892
BtrI CACGTC 1 cut(s) 834
BtsCI GGATG 1 cut(s) 677
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 3 cut(s) 198, 250, 999
Cac8I GCNNGC 3 cut(s) 765, 1029, 1050
CfoI GCGC 4 cut(s) 114, 733, 1029, 1031
Cfr13I GGNCC 1 cut(s) 339
Cfr9I CCCGGG 1 cut(s) 232
Csp6I GTAC 1 cut(s) 958
CviAII CATG 2 cut(s) 418, 894
CviQI GTAC 1 cut(s) 958
DdeI CTNAG 2 cut(s) 58, 723
DpnI GATC 4 cut(s) 411, 483, 816, 858
DpnII GATC 4 cut(s) 409, 481, 814, 856
EaeI YGGCCR 5 cut(s) 131, 195, 247, 262, 771
EagI CGGCCG 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 26, 50, 496
EarI CTCTTC 3 cut(s) 26, 50, 496
EclXI CGGCCG 1 cut(s) 131
Eco130I CCWWGG 3 cut(s) 399, 477, 627
Eco52I CGGCCG 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 796
EcoT14I CCWWGG 3 cut(s) 399, 477, 627
ErhI CCWWGG 3 cut(s) 399, 477, 627
FaeI CATG 2 cut(s) 421, 897
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 1 cut(s) 1010
FatI CATG 2 cut(s) 417, 893
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 1 cut(s) 373
Fnu4HI GCNGC 5 cut(s) 87, 131, 693, 761, 898
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 5 cut(s) 87, 131, 693, 761, 898
FspBI CTAG 4 cut(s) 77, 288, 734, 860
GlaI GCGC 4 cut(s) 113, 732, 1028, 1030
GluI GCNGC 5 cut(s) 87, 131, 693, 761, 898
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 7 cut(s) 133, 197, 249, 264, 341, 773, 892
HapII CCGG 1 cut(s) 233
HhaI GCGC 4 cut(s) 114, 733, 1029, 1031
Hin1II CATG 2 cut(s) 421, 897
Hin6I GCGC 4 cut(s) 112, 731, 1027, 1029
HinP1I GCGC 4 cut(s) 112, 731, 1027, 1029
HinfI GANTC 4 cut(s) 158, 222, 592, 980
HpaII CCGG 1 cut(s) 233
HphI GGTGA 5 cut(s) 83, 265, 499, 532, 998
Hpy166II GTNNAC 4 cut(s) 607, 831, 937, 960
Hpy188I TCNGA 2 cut(s) 169, 259
Hpy188III TCNNGA 6 cut(s) 60, 442, 589, 812, 844, 977
Hpy8I GTNNAC 4 cut(s) 607, 831, 937, 960
Hpy99I CGWCG 2 cut(s) 139, 838
HpyAV CCTTC 2 cut(s) 583, 690
HpyCH4III ACNGT 2 cut(s) 511, 604
HpyCH4IV ACGT 2 cut(s) 616, 833
HpyCH4V TGCA 5 cut(s) 714, 760, 900, 986, 1048
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 1049
HpyF3I CTNAG 2 cut(s) 58, 723
HpySE526I ACGT 2 cut(s) 616, 833
Hsp92II CATG 2 cut(s) 421, 897
HspAI GCGC 4 cut(s) 112, 731, 1027, 1029
Kzo9I GATC 4 cut(s) 409, 481, 814, 856
Lsp1109I GCAGC 4 cut(s) 98, 704, 772, 884
MaeI CTAG 4 cut(s) 77, 288, 734, 860
MaeII ACGT 2 cut(s) 616, 833
MaeIII GTNAC 5 cut(s) 505, 518, 538, 598, 649
MalI GATC 4 cut(s) 411, 483, 816, 858
MboI GATC 4 cut(s) 409, 481, 814, 856
MboII GAAGA 8 cut(s) 13, 37, 430, 460, 476, 483, 668, 1073
MflI RGATCY 3 cut(s) 409, 481, 856
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 2 cut(s) 197, 773
MluCI AATT 2 cut(s) 364, 796
MluNI TGGCCA 2 cut(s) 197, 773
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 730
Mox20I TGGCCA 2 cut(s) 197, 773
MscI TGGCCA 2 cut(s) 197, 773
MseI TTAA 4 cut(s) 303, 393, 887, 1072
MslI CAYNNNNRTG 1 cut(s) 836
Msp20I TGGCCA 2 cut(s) 197, 773
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 2 cut(s) 233, 234
Mva1269I GAATGC 1 cut(s) 986
MvnI CGCG 2 cut(s) 731, 1029
MwoI GCNNNNNNNGC 2 cut(s) 139, 1049
NciI CCSGG 2 cut(s) 233, 234
NdeI CATATG 1 cut(s) 373
NdeII GATC 4 cut(s) 409, 481, 814, 856
NlaIII CATG 2 cut(s) 421, 897
NlaIV GGNNCC 2 cut(s) 340, 411
NmuCI GTSAC 3 cut(s) 505, 538, 649
OliI CACNNNNGTG 1 cut(s) 836
PauI GCGCGC 1 cut(s) 1027
PctI GAATGC 1 cut(s) 986
PfeI GAWTC 3 cut(s) 222, 592, 980
PflFI GACNNNGTC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 204
PkrI GCNGC 5 cut(s) 88, 132, 694, 762, 899
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PshAI GACNNNNGTC 1 cut(s) 536
PspN4I GGNNCC 2 cut(s) 340, 411
PspPI GGNCC 1 cut(s) 339
PsuI RGATCY 3 cut(s) 409, 481, 856
PsyI GACNNNGTC 1 cut(s) 464
PteI GCGCGC 1 cut(s) 1027
RsaI GTAC 1 cut(s) 959
RsaNI GTAC 1 cut(s) 958
RseI CAYNNNNRTG 1 cut(s) 836
SaqAI TTAA 4 cut(s) 303, 393, 887, 1072
SatI GCNGC 5 cut(s) 87, 131, 693, 761, 898
Sau3AI GATC 4 cut(s) 409, 481, 814, 856
Sau96I GGNCC 1 cut(s) 339
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 2 cut(s) 233, 234
SduI GDGCHC 1 cut(s) 689
SmaI CCCGGG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 836
Sse9I AATT 2 cut(s) 364, 796
SsiI CCGC 2 cut(s) 130, 515
SspI AATATT 1 cut(s) 920
SspMI CTAG 4 cut(s) 77, 288, 734, 860
StyD4I CCNGG 2 cut(s) 231, 232
StyI CCWWGG 3 cut(s) 399, 477, 627
TaaI ACNGT 2 cut(s) 511, 604
TaiI ACGT 2 cut(s) 619, 836
TaqI TCGA 1 cut(s) 683
TasI AATT 2 cut(s) 364, 796
TatI WGTACW 1 cut(s) 957
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 3 cut(s) 222, 592, 980
Tru1I TTAA 4 cut(s) 303, 393, 887, 1072
Tru9I TTAA 4 cut(s) 303, 393, 887, 1072
TscAI CASTG 3 cut(s) 198, 250, 1006
TseFI GTSAC 3 cut(s) 505, 538, 649
TseI GCWGC 4 cut(s) 86, 692, 760, 897
Tsp45I GTSAC 3 cut(s) 505, 538, 649
TspDTI ATGAA 4 cut(s) 17, 565, 690, 930
TspMI CCCGGG 1 cut(s) 232
TspRI CASTG 3 cut(s) 198, 250, 1006
Tth111I GACNNNGTC 1 cut(s) 464
Van91I CCANNNNNTGG 1 cut(s) 204
XapI RAATTY 2 cut(s) 364, 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 713
XmaI CCCGGG 1 cut(s) 232
XspI CTAG 4 cut(s) 77, 288, 734, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.