MD07G1066000.v1.1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001140
Physical Location & Seq
Forward (+)
6222074 .. 6226674
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1066000.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGAGAAGAAACCCACCGTCGCCTCTTCATTTTGTCTCCTTCTTTTATGTGTCGCCACTTCAACTGTAGCAGAAGTTCTTGAGGCACACCCCCACCTTCACCACCAACCTCACAAAAATGATGGTTCCATCAAACTTTTCGTATTCGGGGACTCGTATGCTGATACCGGGAATGTCAGAAAATCTGTATCTCCTTCGTGGAAGGAACCTTATGGGATCACATTTCCTGGAAAGCCTGCCGGCCGCTTTTCCGATGGTCGTGTGCTCACTGATTATTTGGCTCAATTTTTGGGTATAAGATCTCCATTACCTTACGCATTGAGAAAATATGCGAAGAAATCGAAACTGGAGAACGGGATGAACTTTGCATATGGAGGGACGGGAGTTTTCGATACGTTGGTGAGTGGACCAAACTTGACCACCCAAATCGGCTTTTTCCAACGGCTGCTTGAACAAGAAAAAGTGTATGACAAACGCGACTTAATTAAATCCTCCGTAGCTCTAGTGTCAGTTGCGGGGAACGACTACACAACTCATTTCGTTAAACCAGGCAATGGCAGTAAGGATTTGGCAGAGTTCACCAAATCGATTGTTAAGCAGATTGCTGTGGATTTGATGCGTATACGTGATTTGGGAGTGCGAAAAATAGCAGTGACAGCACTAGAGCCCATGGGATGTCTGCCGCTCTTAACTTCCTTCCTTTCCTATCAGAACTGCAGCGAAACGCTCAACTTGGCGTCGATGTTCCACAACCAAGTTTTGCGCCAAAACGTGGAACAGTTGAATAAGGAGTCGAAGAAATCTGCATTTATAATCCTGGATCTCTATGGCGCATTTTTGTCTGCAATTAAGCCCCAGAAAGACCATCAAGGGAAAATGATGGTCCAGATTGATGATCCACTGAAACCGTGCTGTGTCGGGGTGGGCAGCGAATACTCATGTGGGAGTGTGGACGAGAGTACCGGAGCAAAGAAGTACGGCATATGCAGTAATCCTGAGAGGTCCTTCTTTTGGGACACGGTGCACCTCTCTCAGAATGGTTGGAATGATGTTTATTCCTCTTTGAAATCTTCTCTTCATCAATTGTATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

40.29

Weight (kDa)

8.84

Isoelectric Point (pI)

23.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 347 6.8e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
AccB7I CCANNNNNTGG 2 cut(s) 554, 772
AccBSI CCGCTC 1 cut(s) 685
AccI GTMKAC 1 cut(s) 622
AccII CGCG 1 cut(s) 477
AciI CCGC 3 cut(s) 244, 515, 683
AclWI GGATC 3 cut(s) 224, 828, 890
AcoI YGGCCR 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 737
AfaI GTAC 2 cut(s) 961, 977
AfiI CCNNNNNNNGG 3 cut(s) 554, 772, 1011
AgsI TTSAA 4 cut(s) 63, 452, 784, 1066
AjnI CCWGG 3 cut(s) 226, 547, 816
AluBI AGCT 1 cut(s) 500
AluI AGCT 1 cut(s) 500
Alw21I GWGCWC 2 cut(s) 267, 1026
Alw26I GTCTC 1 cut(s) 41
Alw44I GTGCAC 1 cut(s) 1022
AlwI GGATC 3 cut(s) 224, 828, 890
AoxI GGCC 1 cut(s) 241
ApaLI GTGCAC 1 cut(s) 1022
ApeKI GCWGC 3 cut(s) 445, 717, 927
AspLEI GCGC 2 cut(s) 765, 833
AspS9I GGNCC 3 cut(s) 407, 883, 1002
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 3 cut(s) 92, 412, 571
AvaII GGWCC 3 cut(s) 407, 883, 1002
BaeGI GKGCMC 1 cut(s) 1026
BaeI ACNNNNGTAYC 4 cut(s) 384, 417, 943, 976
BanII GRGCYC 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 267, 1026
BbvI GCAGC 3 cut(s) 432, 729, 939
BccI CCATC 5 cut(s) 116, 137, 248, 873, 874
BceAI ACGGC 2 cut(s) 458, 994
BciT130I CCWGG 3 cut(s) 228, 549, 818
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 2 cut(s) 503, 662
BfmI CTRYAG 2 cut(s) 66, 715
BglII AGATCT 1 cut(s) 299
BisI GCNGC 5 cut(s) 244, 446, 683, 718, 928
BlsI GCNGC 5 cut(s) 245, 447, 684, 719, 929
Bme1390I CCNGG 4 cut(s) 169, 228, 549, 818
Bme18I GGWCC 3 cut(s) 407, 883, 1002
BmgT120I GGNCC 3 cut(s) 407, 883, 1002
BmiI GGNNCC 2 cut(s) 127, 207
BmrFI CCNGG 4 cut(s) 169, 228, 549, 818
BmsI GCATC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 169
Bsa29I ATCGAT 1 cut(s) 587
BsaAI YACGTR 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 737
BsaJI CCNNGG 1 cut(s) 669
BsaWI WCCGGW 1 cut(s) 962
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 772, 1011
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 559
BseBI CCWGG 3 cut(s) 228, 549, 818
BseCI ATCGAT 1 cut(s) 587
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 2 cut(s) 363, 680
BseLI CCNNNNNNNGG 3 cut(s) 554, 772, 1011
BseMI GCAATG 1 cut(s) 559
BseMII CTCAG 2 cut(s) 987, 1046
BseNI ACTGG 1 cut(s) 351
BseSI GKGCMC 1 cut(s) 1026
BseX3I CGGCCG 1 cut(s) 241
BseXI GCAGC 3 cut(s) 432, 729, 939
Bsh1236I CGCG 1 cut(s) 477
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 243
BshVI ATCGAT 1 cut(s) 587
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 267, 1026
BsiSI CCGG 3 cut(s) 168, 240, 963
BslFI GGGAC 3 cut(s) 164, 391, 1028
BslI CCNNNNNNNGG 3 cut(s) 554, 772, 1011
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 164, 391, 1028
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 3 cut(s) 267, 669, 1026
Bsp143I GATC 4 cut(s) 216, 299, 820, 895
Bsp19I CCATGG 1 cut(s) 669
BspACI CCGC 3 cut(s) 244, 515, 683
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 988, 1045
BspDI ATCGAT 1 cut(s) 587
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 127, 207
BspMAI CTGCAG 1 cut(s) 719
BspPI GGATC 3 cut(s) 224, 828, 890
BsrBI CCGCTC 1 cut(s) 685
BsrDI GCAATG 1 cut(s) 559
BsrFI RCCGGY 1 cut(s) 239
BsrI ACTGG 1 cut(s) 351
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 4 cut(s) 216, 299, 820, 895
BssNAI GTATAC 1 cut(s) 623
BssNI GRCGYC 1 cut(s) 737
BssT1I CCWWGG 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 623
Bst2UI CCWGG 3 cut(s) 228, 549, 818
Bst4CI ACNGT 5 cut(s) 19, 67, 780, 909, 1021
Bst6I CTCTTC 2 cut(s) 31, 1080
BstACI GRCGYC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 237, 241
BstDEI CTNAG 2 cut(s) 996, 1032
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 2 cut(s) 363, 680
BstFNI CGCG 1 cut(s) 477
BstHHI GCGC 2 cut(s) 765, 833
BstKTI GATC 4 cut(s) 219, 302, 823, 898
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 4 cut(s) 216, 299, 820, 895
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 656
BstNI CCWGG 3 cut(s) 228, 549, 818
BstSCI CCNGG 4 cut(s) 167, 226, 547, 816
BstSFI CTRYAG 2 cut(s) 66, 715
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 3 cut(s) 432, 729, 939
BstX2I RGATCY 2 cut(s) 299, 820
BstYI RGATCY 2 cut(s) 299, 820
BstZ17I GTATAC 1 cut(s) 623
BstZI CGGCCG 1 cut(s) 241
Bsu15I ATCGAT 1 cut(s) 587
BsuRI GGCC 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 587
BtgI CCRYGG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 363, 680
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 3 cut(s) 267, 657, 899
Cac8I GCNNGC 2 cut(s) 237, 241
CfoI GCGC 2 cut(s) 765, 833
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 407, 883, 1002
ClaI ATCGAT 1 cut(s) 587
CseI GACGC 1 cut(s) 726
Csp6I GTAC 2 cut(s) 960, 976
CviAII CATG 2 cut(s) 670, 939
CviJI RGCY 8 cut(s) 235, 243, 281, 432, 445, 500, 667, 853
CviKI_1 RGCY 8 cut(s) 235, 243, 281, 432, 445, 500, 667, 853
CviQI GTAC 2 cut(s) 960, 976
DdeI CTNAG 2 cut(s) 996, 1032
DpnI GATC 4 cut(s) 218, 301, 822, 897
DpnII GATC 4 cut(s) 216, 299, 820, 895
EaeI YGGCCR 1 cut(s) 241
EagI CGGCCG 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 31, 1080
EarI CTCTTC 2 cut(s) 31, 1080
EclXI CGGCCG 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 669
Eco47I GGWCC 3 cut(s) 407, 883, 1002
Eco52I CGGCCG 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 1002
EcoRII CCWGG 3 cut(s) 226, 547, 816
EcoT14I CCWWGG 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 2 cut(s) 673, 942
FaqI GGGAC 3 cut(s) 164, 391, 1028
FatI CATG 2 cut(s) 669, 938
FauI CCCGC 1 cut(s) 508
FauNDI CATATG 2 cut(s) 370, 983
FblI GTMKAC 1 cut(s) 622
Fnu4HI GCNGC 5 cut(s) 244, 446, 683, 718, 928
FokI GGATG 2 cut(s) 370, 687
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 5 cut(s) 244, 446, 683, 718, 928
FspBI CTAG 2 cut(s) 503, 662
GlaI GCGC 2 cut(s) 764, 832
GluI GCNGC 5 cut(s) 244, 446, 683, 718, 928
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 243
HapII CCGG 3 cut(s) 168, 240, 963
HgaI GACGC 1 cut(s) 726
HhaI GCGC 2 cut(s) 765, 833
Hin1I GRCGYC 1 cut(s) 737
Hin1II CATG 2 cut(s) 673, 942
Hin6I GCGC 2 cut(s) 763, 831
HinP1I GCGC 2 cut(s) 763, 831
HinfI GANTC 2 cut(s) 152, 791
HpaII CCGG 3 cut(s) 168, 240, 963
HphI GGTGA 3 cut(s) 92, 412, 571
Hpy166II GTNNAC 5 cut(s) 407, 579, 623, 952, 1024
Hpy188I TCNGA 4 cut(s) 179, 253, 711, 1035
Hpy188III TCNNGA 4 cut(s) 80, 886, 995, 1092
Hpy8I GTNNAC 5 cut(s) 407, 579, 623, 952, 1024
Hpy99I CGWCG 2 cut(s) 23, 742
HpyAV CCTTC 6 cut(s) 50, 107, 196, 204, 706, 1015
HpyCH4III ACNGT 5 cut(s) 19, 67, 780, 909, 1021
HpyCH4IV ACGT 3 cut(s) 395, 625, 771
HpyCH4V TGCA 6 cut(s) 368, 717, 806, 845, 987, 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 656
HpyF3I CTNAG 2 cut(s) 996, 1032
HpySE526I ACGT 3 cut(s) 395, 625, 771
Hsp92I GRCGYC 1 cut(s) 737
Hsp92II CATG 2 cut(s) 673, 942
HspAI GCGC 2 cut(s) 763, 831
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 4 cut(s) 216, 299, 820, 895
LmnI GCTCC 1 cut(s) 965
Lsp1109I GCAGC 3 cut(s) 432, 729, 939
LweI GCATC 1 cut(s) 606
MaeI CTAG 2 cut(s) 503, 662
MaeII ACGT 3 cut(s) 395, 625, 771
MaeIII GTNAC 1 cut(s) 652
MalI GATC 4 cut(s) 218, 301, 822, 897
MbiI CCGCTC 1 cut(s) 685
MboI GATC 4 cut(s) 216, 299, 820, 895
MboII GAAGA 6 cut(s) 18, 19, 346, 808, 1062, 1067
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 2 cut(s) 299, 820
MhlI GDGCHC 3 cut(s) 267, 669, 1026
MluCI AATT 4 cut(s) 284, 483, 846, 1082
MlyI GAGTC 2 cut(s) 146, 800
MmeI TCCRAC 2 cut(s) 463, 1022
MnlI CCTC 8 cut(s) 34, 76, 120, 368, 502, 993, 1037, 1069
MroNI GCCGGC 1 cut(s) 239
MseI TTAA 6 cut(s) 482, 486, 543, 594, 689, 849
MslI CAYNNNNRTG 1 cut(s) 117
MspI CCGG 3 cut(s) 168, 240, 963
MspR9I CCNGG 4 cut(s) 169, 228, 549, 818
MunI CAATTG 1 cut(s) 1082
MvaI CCWGG 3 cut(s) 228, 549, 818
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 656
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 169
NcoI CCATGG 1 cut(s) 669
NdeI CATATG 2 cut(s) 370, 983
NdeII GATC 4 cut(s) 216, 299, 820, 895
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 2 cut(s) 673, 942
NlaIV GGNNCC 2 cut(s) 127, 207
NmuCI GTSAC 1 cut(s) 652
PacI TTAATTAA 1 cut(s) 486
PdiI GCCGGC 1 cut(s) 241
PflMI CCANNNNNTGG 2 cut(s) 554, 772
PfoI TCCNGGA 2 cut(s) 226, 816
PkrI GCNGC 5 cut(s) 245, 447, 684, 719, 929
PleI GAGTC 2 cut(s) 146, 799
PpsI GAGTC 2 cut(s) 146, 799
Ppu21I YACGTR 1 cut(s) 626
PpuMI RGGWCCY 1 cut(s) 1002
PsiI TTATAA 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 1002
Psp6I CCWGG 3 cut(s) 226, 547, 816
PspGI CCWGG 3 cut(s) 226, 547, 816
PspN4I GGNNCC 2 cut(s) 127, 207
PspPI GGNCC 3 cut(s) 407, 883, 1002
PspPPI RGGWCCY 1 cut(s) 1002
PsrI GAACNNNNNNTAC 2 cut(s) 60, 92
PstI CTGCAG 1 cut(s) 719
PsuI RGATCY 2 cut(s) 299, 820
RsaI GTAC 2 cut(s) 961, 977
RsaNI GTAC 2 cut(s) 960, 976
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 6 cut(s) 482, 486, 543, 594, 689, 849
SatI GCNGC 5 cut(s) 244, 446, 683, 718, 928
Sau3AI GATC 4 cut(s) 216, 299, 820, 895
Sau96I GGNCC 3 cut(s) 407, 883, 1002
SchI GAGTC 2 cut(s) 146, 800
ScrFI CCNGG 4 cut(s) 169, 228, 549, 818
SduI GDGCHC 3 cut(s) 267, 669, 1026
SfaNI GCATC 1 cut(s) 606
SfcI CTRYAG 2 cut(s) 66, 715
SinI GGWCC 3 cut(s) 407, 883, 1002
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 4 cut(s) 284, 483, 846, 1082
SsiI CCGC 3 cut(s) 244, 515, 683
SspMI CTAG 2 cut(s) 503, 662
StyD4I CCNGG 4 cut(s) 167, 226, 547, 816
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 5 cut(s) 19, 67, 780, 909, 1021
TaiI ACGT 3 cut(s) 398, 628, 774
TaqI TCGA 5 cut(s) 341, 390, 587, 740, 794
TasI AATT 4 cut(s) 284, 483, 846, 1082
TauI GCSGC 2 cut(s) 246, 685
Tru1I TTAA 6 cut(s) 482, 486, 543, 594, 689, 849
Tru9I TTAA 6 cut(s) 482, 486, 543, 594, 689, 849
TscAI CASTG 3 cut(s) 274, 657, 906
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 3 cut(s) 445, 717, 927
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 3 cut(s) 18, 374, 1067
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 3 cut(s) 274, 657, 906
Van91I CCANNNNNTGG 2 cut(s) 554, 772
VneI GTGCAC 1 cut(s) 1022
VpaK11BI GGWCC 3 cut(s) 407, 883, 1002
XmiI GTMKAC 1 cut(s) 622
XspI CTAG 2 cut(s) 503, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.