Rw1G010490

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001140
Physical Location & Seq
Reverse (-)
23489756 .. 23495330
5575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010490.1

Sequence Viewer

Length: 1017 bp
ATGGAGCAAACCACCATCGTCTCATTCTGTCTCTTTCTTCTCTACATCTCCACTTGTCCACAACTTTTTGTTTTCGGGGATTCGTATGCTGATACTGGAAACAGCGGCAAATCTGCAACTGGTTCGTGGAAGCCGCCTTATGGGATCACTTTTCCCGGAAAGCCGGCAGGCCGCTTCTCCGATGGTCGCGTGCTCACTGATTATATAGCTTCCTTTTTGGGTATAAGATCTCTAGTACCTTACGAATTGAGAAACACTGAAGAGAAATCGAAACAAAGGAACGGGATGAACTTTGCATATGGAGGAACTGGTGTTTTTGAGACTTTGGTGAGTGCACCAAACATGACTACCCAAATCGACTTTCTCCAACAACTAGTCGAAGAGAAATTGTATACCAAAAGTGACTTGGACTCGTCCGTTGCTTTAGTATCACTAGGTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCAGAGTCAGGATTTTTCAGCGGTGTCCACATCGATTGTCGACCAGCTTGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGAAAATTATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGTAACTTCCATTTTTTCTTCGTATCAGAATTGCAGTGAAAGTATGAATGCAGGTTCAAGATTCCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTAAAGAATCAGACAACTACGAGATCAACATTTGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATCCAACAACAAAAACACCTGCAACAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAAAAGTGGAGCAAACAAGTACACAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACATGGAGCACCTTTCGCAGAATGGCTGGCATGCTGTTTATTCTGCAATCAAGACTTCTCTTCGTCCATTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

338

Amino Acids

37.4

Weight (kDa)

6.52

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 22 - 329 7.4e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 798
Acc36I ACCTGC 2 cut(s) 647, 798
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 2 cut(s) 392, 513
AccII CGCG 1 cut(s) 189
AccIII TCCGGA 1 cut(s) 919
AciI CCGC 5 cut(s) 105, 134, 172, 494, 768
AclWI GGATC 2 cut(s) 152, 753
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 1 cut(s) 279
AdeI CACNNNGTG 2 cut(s) 437, 909
AfaI GTAC 2 cut(s) 237, 902
AfiI CCNNNNNNNGG 3 cut(s) 140, 481, 523
AgsI TTSAA 2 cut(s) 663, 698
AhlI ACTAGT 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 345, 377
AluBI AGCT 2 cut(s) 209, 520
AluI AGCT 2 cut(s) 209, 520
Alw21I GWGCWC 3 cut(s) 195, 337, 951
Alw26I GTCTC 3 cut(s) 25, 35, 314
Alw44I GTGCAC 1 cut(s) 333
AlwI GGATC 2 cut(s) 152, 753
Aor13HI TCCGGA 1 cut(s) 919
AoxI GGCC 1 cut(s) 169
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 452, 852
ApoI RAATTY 1 cut(s) 802
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 340, 538, 859
BaeGI GKGCMC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 588
BauI CACGAG 1 cut(s) 862
Bbv12I GWGCWC 3 cut(s) 195, 337, 951
BbvI GCAGC 2 cut(s) 439, 864
BccI CCATC 2 cut(s) 23, 176
BceAI ACGGC 1 cut(s) 590
BcgI CGANNNNNNTGC 4 cut(s) 105, 139, 434, 468
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 3 cut(s) 25, 35, 314
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 3 cut(s) 233, 374, 434
BfuAI ACCTGC 2 cut(s) 647, 798
BglII AGATCT 1 cut(s) 227
BisI GCNGC 5 cut(s) 106, 134, 172, 453, 853
BlsI GCNGC 5 cut(s) 107, 135, 173, 454, 854
Bme1390I CCNGG 1 cut(s) 156
BmrFI CCNGG 1 cut(s) 156
BmsI GCATC 1 cut(s) 819
BpuMI CCSGG 1 cut(s) 156
Bsa29I ATCGAT 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 588
BsaWI WCCGGW 1 cut(s) 919
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 481, 523
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 3 cut(s) 100, 124, 313
Bse3DI GCAATG 1 cut(s) 1007
BseAI TCCGGA 1 cut(s) 919
BseCI ATCGAT 1 cut(s) 506
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 2 cut(s) 291, 599
BseLI CCNNNNNNNGG 3 cut(s) 140, 481, 523
BseMI GCAATG 1 cut(s) 1007
BseNI ACTGG 3 cut(s) 100, 124, 313
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 2 cut(s) 439, 864
BsgI GTGCAG 1 cut(s) 931
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 506
BsiHKAI GWGCWC 3 cut(s) 195, 337, 951
BsiSI CCGG 3 cut(s) 156, 164, 920
BslFI GGGAC 1 cut(s) 953
BslI CCNNNNNNNGG 3 cut(s) 140, 481, 523
BsmAI GTCTC 3 cut(s) 25, 35, 314
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 953
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 1 cut(s) 171
Bsp1286I GDGCHC 4 cut(s) 195, 337, 588, 951
Bsp13I TCCGGA 1 cut(s) 919
Bsp143I GATC 5 cut(s) 144, 227, 725, 745, 812
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 5 cut(s) 105, 134, 172, 494, 768
BspANI GGCC 1 cut(s) 171
BspDI ATCGAT 1 cut(s) 506
BspEI TCCGGA 1 cut(s) 919
BspFNI CGCG 1 cut(s) 189
BspMI ACCTGC 2 cut(s) 647, 798
BspPI GGATC 2 cut(s) 152, 753
BsrDI GCAATG 1 cut(s) 1007
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 3 cut(s) 100, 124, 313
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 5 cut(s) 144, 227, 725, 745, 812
BssNAI GTATAC 1 cut(s) 393
BssSI CACGAG 1 cut(s) 862
BssT1I CCWWGG 1 cut(s) 588
Bst1107I GTATAC 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 862
Bst4CI ACNGT 1 cut(s) 907
Bst6I CTCTTC 3 cut(s) 255, 375, 1005
BstC8I GCNNGC 6 cut(s) 165, 169, 191, 603, 968, 972
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 291, 599
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 5 cut(s) 147, 230, 728, 748, 815
BstMAI GTCTC 3 cut(s) 25, 35, 314
BstMBI GATC 5 cut(s) 144, 227, 725, 745, 812
BstMWI GCNNNNNNNGC 1 cut(s) 955
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 1 cut(s) 154
BstSLI GKGCMC 1 cut(s) 337
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 439, 864
BstX2I RGATCY 2 cut(s) 227, 745
BstYI RGATCY 2 cut(s) 227, 745
BstZ17I GTATAC 1 cut(s) 393
Bsu15I ATCGAT 1 cut(s) 506
BsuRI GGCC 1 cut(s) 171
BsuTUI ATCGAT 1 cut(s) 506
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 2 cut(s) 291, 599
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 5 cut(s) 195, 255, 646, 824, 912
BveI ACCTGC 2 cut(s) 647, 798
Cac8I GCNNGC 6 cut(s) 165, 169, 191, 603, 968, 972
Cfr10I RCCGGY 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 506
Csp6I GTAC 2 cut(s) 236, 901
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 5 cut(s) 343, 589, 834, 943, 971
CviJI RGCY 8 cut(s) 133, 163, 171, 209, 520, 586, 605, 966
CviKI_1 RGCY 8 cut(s) 133, 163, 171, 209, 520, 586, 605, 966
CviQI GTAC 2 cut(s) 236, 901
DpnI GATC 5 cut(s) 146, 229, 727, 747, 814
DpnII GATC 5 cut(s) 144, 227, 725, 745, 812
DraI TTTAAA 1 cut(s) 705
DraIII CACNNNGTG 2 cut(s) 437, 909
Eam1104I CTCTTC 3 cut(s) 255, 375, 1005
EarI CTCTTC 3 cut(s) 255, 375, 1005
Eco130I CCWWGG 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 588
Eco57I CTGAAG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 588
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 588
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 5 cut(s) 346, 592, 837, 946, 974
FaqI GGGAC 1 cut(s) 953
FatI CATG 5 cut(s) 342, 588, 833, 942, 970
FauNDI CATATG 1 cut(s) 298
FblI GTMKAC 2 cut(s) 392, 513
Fnu4HI GCNGC 5 cut(s) 106, 134, 172, 453, 853
FokI GGATG 2 cut(s) 298, 606
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 5 cut(s) 106, 134, 172, 453, 853
FspBI CTAG 3 cut(s) 233, 374, 434
GluI GCNGC 5 cut(s) 106, 134, 172, 453, 853
HaeIII GGCC 1 cut(s) 171
HapII CCGG 3 cut(s) 156, 164, 920
Hin1II CATG 5 cut(s) 346, 592, 837, 946, 974
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 6 cut(s) 80, 410, 478, 666, 709, 820
HpaII CCGG 3 cut(s) 156, 164, 920
HphI GGTGA 3 cut(s) 340, 538, 859
Hpy166II GTNNAC 8 cut(s) 59, 335, 393, 501, 514, 529, 877, 903
Hpy188I TCNGA 4 cut(s) 181, 633, 714, 817
Hpy188III TCNNGA 4 cut(s) 482, 663, 920, 991
Hpy8I GTNNAC 8 cut(s) 59, 335, 393, 501, 514, 529, 877, 903
HpyCH4III ACNGT 1 cut(s) 907
HpyF10VI GCNNNNNNNGC 1 cut(s) 955
Hsp92II CATG 5 cut(s) 346, 592, 837, 946, 974
Kpn2I TCCGGA 1 cut(s) 919
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Kzo9I GATC 5 cut(s) 144, 227, 725, 745, 812
LmnI GCTCC 3 cut(s) 4, 890, 946
Lsp1109I GCAGC 2 cut(s) 439, 864
LweI GCATC 1 cut(s) 819
MaeI CTAG 3 cut(s) 233, 374, 434
MaeIII GTNAC 3 cut(s) 401, 571, 607
MalI GATC 5 cut(s) 146, 229, 727, 747, 814
MboI GATC 5 cut(s) 144, 227, 725, 745, 812
MboII GAAGA 6 cut(s) 29, 272, 392, 615, 710, 992
MflI RGATCY 2 cut(s) 227, 745
MhlI GDGCHC 4 cut(s) 195, 337, 588, 951
MluCI AATT 5 cut(s) 245, 386, 563, 634, 802
MlyI GAGTC 2 cut(s) 404, 487
MmeI TCCRAC 2 cut(s) 391, 799
MnlI CCTC 1 cut(s) 296
Mph1103I ATGCAT 1 cut(s) 760
MroI TCCGGA 1 cut(s) 919
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 3 cut(s) 465, 704, 1015
MspA1I CMGCKG 2 cut(s) 105, 494
MspI CCGG 3 cut(s) 156, 164, 920
MspR9I CCNGG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 658
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 955
NaeI GCCGGC 1 cut(s) 165
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 588
NdeI CATATG 1 cut(s) 298
NdeII GATC 5 cut(s) 144, 227, 725, 745, 812
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 5 cut(s) 346, 592, 837, 946, 974
NmuCI GTSAC 1 cut(s) 401
NsiI ATGCAT 1 cut(s) 760
NspI RCATGY 1 cut(s) 974
PaeI GCATGC 1 cut(s) 974
PaqCI CACCTGC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 1009
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 4 cut(s) 80, 666, 709, 820
PflMI CCANNNNNTGG 1 cut(s) 523
PfoI TCCNGGA 1 cut(s) 154
PkrI GCNGC 5 cut(s) 107, 135, 173, 454, 854
PleI GAGTC 2 cut(s) 404, 486
PpsI GAGTC 2 cut(s) 404, 486
PsuI RGATCY 2 cut(s) 227, 745
RsaI GTAC 2 cut(s) 237, 902
RsaNI GTAC 2 cut(s) 236, 901
SalI GTCGAC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 465, 704, 1015
SatI GCNGC 5 cut(s) 106, 134, 172, 453, 853
Sau3AI GATC 5 cut(s) 144, 227, 725, 745, 812
SchI GAGTC 2 cut(s) 404, 487
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 4 cut(s) 195, 337, 588, 951
SetI ASST 9 cut(s) 211, 241, 439, 522, 661, 696, 792, 929, 954
SfaNI GCATC 1 cut(s) 819
SpeI ACTAGT 1 cut(s) 373
SphI GCATGC 1 cut(s) 974
Sse9I AATT 5 cut(s) 245, 386, 563, 634, 802
SsiI CCGC 5 cut(s) 105, 134, 172, 494, 768
SspMI CTAG 3 cut(s) 233, 374, 434
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 907
TaqI TCGA 6 cut(s) 269, 357, 378, 506, 513, 806
TasI AATT 5 cut(s) 245, 386, 563, 634, 802
TatI WGTACW 1 cut(s) 900
TauI GCSGC 3 cut(s) 108, 136, 174
TfiI GAWTC 4 cut(s) 80, 666, 709, 820
Tru1I TTAA 3 cut(s) 465, 704, 1015
Tru9I TTAA 3 cut(s) 465, 704, 1015
TscAI CASTG 5 cut(s) 202, 262, 646, 831, 912
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 2 cut(s) 452, 852
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 2 cut(s) 302, 665
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 5 cut(s) 202, 262, 646, 831, 912
Van91I CCANNNNNTGG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 333
XapI RAATTY 1 cut(s) 802
XceI RCATGY 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 2 cut(s) 392, 513
XspI CTAG 3 cut(s) 233, 374, 434
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.