Prupe.2G080500_v2.0.a1

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001140
Physical Location & Seq
Forward (+)
12338461 .. 12343732
5272 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G080500.1

Sequence Viewer

Length: 912 bp
ATGGAGAAGCAAACAGCCATAGCCTCTTCTTGTCTCTTCCTTTTATACATCGCCACGTTTACAGCGGCAGAAGTTCTTGAAGCACATCATCACCATCATCGTCATAATCATCATGGTTCTGTGAAGTTTTTCGTTTTCGAGGAGTCATATGTTGATACTGAAAATATTGAAAAATCTGTGTCTACTTCATGGAAGGAACCTTATGACATCAATTTTTCGAGAAAGCCAGCTGGTCGATTTTCAGATAGTCTCGTGTTCACTGAATATATAACTTCCTTTTTGGGCATAAGATCTCCAGTGCCTTACACATTGAGAAAATTTGTGAAGAAATCAAAACTAGAGTCTGGGATGAACTTTGCATATGGAGGGATAGGTGTTTTTGATAGAGTGTTTGGTGGGCCAAAATCGACCACCCAAATCGACTTTTTCCAACTGCTGCTTGAACAAAAACTCTATAGTACCAAAAACGACGTAGTTAACTCGTCCATTGCTCTCGTATCAGTTGCGGATAACGACTACGCTGCTTATTTTGGCAACCATACTGAGGATTTCGCAGTAGTCACCAAATCCATTATTAAGCAGCTTGCTGTGGATTTAGAGCGCATACATGGGTTGAGAGTGAGAAAAATTGCTGTTACAGCAATAGGGCCCCTGGGATGTCTGCCCCGCATGACTTCCTTTCTTTCATATCAGAATTGTAGTGAAGTTGCCAATTTGGTTTCAATTTTCCATAACCAGATTTTGCGTCAAAAAGTTGAAGAGTTGAACAAGGAGACTAAGAAATCTTCATTTGTAATCTTGGATCTTTATAACGCATCTCTGTCTGCAATTATGCTCCCAAAACACTATCAAGAGGACAAACATCATACAACCAATCCTTGGCAAGGAGAAAGAAACTCCTGCCATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

304

Amino Acids

34.39

Weight (kDa)

8.23

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 810
AccB7I CCANNNNNTGG 1 cut(s) 879
AccI GTMKAC 1 cut(s) 182
AciI CCGC 3 cut(s) 65, 506, 667
AclWI GGATC 1 cut(s) 810
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 3 cut(s) 544, 879, 884
AgsI TTSAA 6 cut(s) 80, 170, 443, 723, 758, 766
AjnI CCWGG 1 cut(s) 651
AluBI AGCT 2 cut(s) 230, 583
AluI AGCT 2 cut(s) 230, 583
Alw26I GTCTC 3 cut(s) 38, 254, 767
AlwI GGATC 1 cut(s) 810
AoxI GGCC 2 cut(s) 398, 647
ApaI GGGCCC 1 cut(s) 651
ApeKI GCWGC 3 cut(s) 436, 521, 580
ApoI RAATTY 1 cut(s) 317
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 128
AspLEI GCGC 1 cut(s) 603
AspS9I GGNCC 3 cut(s) 398, 647, 648
AsuHPI GGTGA 2 cut(s) 83, 553
BaeGI GKGCMC 1 cut(s) 651
BanII GRGCYC 1 cut(s) 651
BauI CACGAG 1 cut(s) 251
BbvI GCAGC 3 cut(s) 423, 508, 592
BccI CCATC 1 cut(s) 102
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 1 cut(s) 653
BcoDI GTCTC 3 cut(s) 38, 254, 767
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 454
BglII AGATCT 1 cut(s) 290
BisI GCNGC 4 cut(s) 66, 437, 522, 581
BlsI GCNGC 4 cut(s) 67, 438, 523, 582
Bme1390I CCNGG 1 cut(s) 653
BmgT120I GGNCC 3 cut(s) 398, 647, 648
BmiI GGNNCC 3 cut(s) 198, 649, 650
BmrFI CCNGG 1 cut(s) 653
BmsI GCATC 1 cut(s) 824
BpmI CTGGAG 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 651, 652, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 879, 884
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 3 cut(s) 651, 652, 878
BseGI GGATG 2 cut(s) 354, 662
BseLI CCNNNNNNNGG 3 cut(s) 544, 879, 884
BseMI GCAATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 534
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 3 cut(s) 423, 508, 592
BshFI GGCC 2 cut(s) 400, 649
BslI CCNNNNNNNGG 3 cut(s) 544, 879, 884
BsmAI GTCTC 3 cut(s) 38, 254, 767
BsnI GGCC 2 cut(s) 400, 649
Bsp120I GGGCCC 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 2 cut(s) 290, 802
BspACI CCGC 3 cut(s) 65, 506, 667
BspANI GGCC 2 cut(s) 400, 649
BspCNI CTCAG 1 cut(s) 535
BspLI GGNNCC 3 cut(s) 198, 649, 650
BspPI GGATC 1 cut(s) 810
BsrDI GCAATG 1 cut(s) 486
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 651, 652, 878
BssMI GATC 2 cut(s) 290, 802
BssSI CACGAG 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 653
Bst6I CTCTTC 3 cut(s) 31, 41, 753
BstC8I GCNNGC 2 cut(s) 228, 585
BstDEI CTNAG 2 cut(s) 543, 777
BstENI CCTNNNNNAGG 1 cut(s) 882
BstF5I GGATG 2 cut(s) 354, 662
BstHHI GCGC 1 cut(s) 603
BstKTI GATC 2 cut(s) 293, 805
BstMAI GTCTC 3 cut(s) 38, 254, 767
BstMBI GATC 2 cut(s) 290, 802
BstMWI GCNNNNNNNGC 1 cut(s) 638
BstNI CCWGG 1 cut(s) 653
BstSCI CCNGG 1 cut(s) 651
BstSFI CTRYAG 1 cut(s) 454
BstSLI GKGCMC 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 423, 508, 592
BstX2I RGATCY 2 cut(s) 290, 802
BstYI RGATCY 2 cut(s) 290, 802
BsuRI GGCC 2 cut(s) 400, 649
BtgZI GCGATG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 354, 662
BtsIMutI CAGTG 2 cut(s) 258, 303
Cac8I GCNNGC 2 cut(s) 228, 585
CfoI GCGC 1 cut(s) 603
Cfr13I GGNCC 3 cut(s) 398, 647, 648
CseI GACGC 1 cut(s) 734
Csp6I GTAC 1 cut(s) 459
CviAII CATG 4 cut(s) 113, 189, 608, 670
CviJI RGCY 7 cut(s) 17, 23, 226, 230, 400, 583, 649
CviKI_1 RGCY 7 cut(s) 17, 23, 226, 230, 400, 583, 649
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 2 cut(s) 543, 777
DpnI GATC 2 cut(s) 292, 804
DpnII GATC 2 cut(s) 290, 802
Eam1104I CTCTTC 3 cut(s) 31, 41, 753
EarI CTCTTC 3 cut(s) 31, 41, 753
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 651
EcoNI CCTNNNNNAGG 1 cut(s) 882
EcoO109I RGGNCCY 2 cut(s) 647, 648
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 1 cut(s) 878
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 878
FaeI CATG 4 cut(s) 116, 192, 611, 673
FatI CATG 4 cut(s) 112, 188, 607, 669
FauI CCCGC 1 cut(s) 674
FauNDI CATATG 2 cut(s) 148, 361
FblI GTMKAC 1 cut(s) 182
Fnu4HI GCNGC 4 cut(s) 66, 437, 522, 581
FokI GGATG 2 cut(s) 361, 669
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 66, 437, 522, 581
FspBI CTAG 1 cut(s) 338
GlaI GCGC 1 cut(s) 602
GluI GCNGC 4 cut(s) 66, 437, 522, 581
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 2 cut(s) 400, 649
HgaI GACGC 1 cut(s) 734
HhaI GCGC 1 cut(s) 603
Hin1II CATG 4 cut(s) 116, 192, 611, 673
Hin6I GCGC 1 cut(s) 601
HinP1I GCGC 1 cut(s) 601
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 2 cut(s) 143, 341
HpaI GTTAAC 1 cut(s) 478
HphI GGTGA 2 cut(s) 83, 553
Hpy166II GTNNAC 4 cut(s) 60, 183, 258, 478
Hpy188I TCNGA 2 cut(s) 244, 693
Hpy188III TCNNGA 3 cut(s) 77, 219, 851
Hpy8I GTNNAC 4 cut(s) 60, 183, 258, 478
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 56, 471
HpyCH4V TGCA 2 cut(s) 359, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 638
HpyF3I CTNAG 2 cut(s) 543, 777
HpySE526I ACGT 2 cut(s) 56, 471
Hsp92II CATG 4 cut(s) 116, 192, 611, 673
HspAI GCGC 1 cut(s) 601
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 2 cut(s) 290, 802
LmnI GCTCC 1 cut(s) 840
LpnPI CCDG 7 cut(s) 216, 240, 309, 330, 638, 665, 749
Lsp1109I GCAGC 3 cut(s) 423, 508, 592
LweI GCATC 1 cut(s) 824
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 56, 471
MaeIII GTNAC 2 cut(s) 559, 634
MalI GATC 2 cut(s) 292, 804
MboI GATC 2 cut(s) 290, 802
MboII GAAGA 5 cut(s) 18, 28, 337, 770, 777
MflI RGATCY 2 cut(s) 290, 802
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 7 cut(s) 211, 317, 627, 694, 712, 723, 828
MlyI GAGTC 2 cut(s) 152, 350
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 5 cut(s) 34, 133, 359, 538, 847
MroXI GAANNNNTTC 1 cut(s) 128
MseI TTAA 3 cut(s) 477, 576, 910
MspA1I CMGCKG 2 cut(s) 65, 230
MspR9I CCNGG 1 cut(s) 653
MvaI CCWGG 1 cut(s) 653
MwoI GCNNNNNNNGC 1 cut(s) 638
NdeI CATATG 2 cut(s) 148, 361
NdeII GATC 2 cut(s) 290, 802
NlaIII CATG 4 cut(s) 116, 192, 611, 673
NlaIV GGNNCC 3 cut(s) 198, 649, 650
NmuCI GTSAC 1 cut(s) 559
PasI CCCWGGG 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 879
PkrI GCNGC 4 cut(s) 67, 438, 523, 582
PleI GAGTC 2 cut(s) 151, 349
PpsI GAGTC 2 cut(s) 151, 349
PsiI TTATAA 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 651
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 3 cut(s) 198, 649, 650
PspOMI GGGCCC 1 cut(s) 647
PspPI GGNCC 3 cut(s) 398, 647, 648
PsuI RGATCY 2 cut(s) 290, 802
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SaqAI TTAA 3 cut(s) 477, 576, 910
SatI GCNGC 4 cut(s) 66, 437, 522, 581
Sau3AI GATC 2 cut(s) 290, 802
Sau96I GGNCC 3 cut(s) 398, 647, 648
SchI GAGTC 2 cut(s) 152, 350
ScrFI CCNGG 1 cut(s) 653
SduI GDGCHC 1 cut(s) 651
SetI ASST 6 cut(s) 59, 202, 232, 376, 474, 585
SfaNI GCATC 1 cut(s) 824
SfcI CTRYAG 1 cut(s) 454
Sse9I AATT 7 cut(s) 211, 317, 627, 694, 712, 723, 828
SsiI CCGC 3 cut(s) 65, 506, 667
SspI AATATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 651
StyI CCWWGG 1 cut(s) 878
TaiI ACGT 2 cut(s) 59, 474
TaqI TCGA 5 cut(s) 138, 218, 235, 407, 420
TasI AATT 7 cut(s) 211, 317, 627, 694, 712, 723, 828
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 477, 576, 910
Tru9I TTAA 3 cut(s) 477, 576, 910
TscAI CASTG 2 cut(s) 265, 303
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 436, 521, 580
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 4 cut(s) 177, 365, 675, 777
TspRI CASTG 2 cut(s) 265, 303
Van91I CCANNNNNTGG 1 cut(s) 879
XagI CCTNNNNNAGG 1 cut(s) 882
XapI RAATTY 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 128
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.