pycom02g21640

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001140
Physical Location & Seq
Reverse (-)
19882145 .. 19883933
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21640.2

Sequence Viewer

Length: 738 bp
ATGAACTTTGCATACGGAGGTACGGGAGTTTTCGATACGTTGGTGACTGAACCAAACTTGACCACCCAAATCGACTTTTTCCAACAGCTAGTTGAACAAGAAAAACTGTATGCCAAACGCGACCTAATTAAATCGTCCATAGCTCTCGTGTCAGTTGCGGGGAACGACTACGCAACTCATATGGTTAAAACAGGCAATGGCAGTGAGGATTTGGCAGAGTTCACCAAATCGGTTGTTAAGCAGATTGCTGTGGATTTGAGGCGTATACGTGATTTGGGAGTGCCAAAAATAGCGGTTACTGCAGTAGAGCCCATGGGATGTATTCCGCGCTTAACTTCCCTTCTTTCCTATCAGAACTGCAGTGAATCGCTCAACTTGGCTTCAGTGTTCCACAACCAAGTTTTGCGCCAAAACGTGGAACAGTTGAATAAGGAGTCAAACAATTCTGCATTTGTAATCTTGGATCTCTATGACGCATTTTCGTCTGCAATTAATCCCCAAAAAGACCGTCAAGGAAACACGACGTTCCAGATTGATGAACCATTAAAACCATGCTGTGTGGGGGTGAGCAGCGAGTACTCATGCGGTAGTGTGGACGAGAGTACTGGAGCAAAGAAGTACAGCATATGCAGTCATCCCGAACGGTCTTTCTTTTGGGACACGGTGCACCCCTCGCAGAATGGCTGGCATGCTGTTTATTCCTCTTTGAAATCTTCTCTTCATCAGTTGTATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

246

Amino Acids

27.15

Weight (kDa)

5.68

Isoelectric Point (pI)

34.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 415
AccI GTMKAC 1 cut(s) 265
AccII CGCG 2 cut(s) 120, 328
AciI CCGC 4 cut(s) 158, 293, 326, 585
AclWI GGATC 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 4 cut(s) 22, 578, 604, 620
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 3 cut(s) 95, 427, 709
AluBI AGCT 2 cut(s) 88, 143
AluI AGCT 2 cut(s) 88, 143
Alw21I GWGCWC 1 cut(s) 669
Alw44I GTGCAC 1 cut(s) 665
AlwI GGATC 1 cut(s) 471
ApaLI GTGCAC 1 cut(s) 665
ApeKI GCWGC 1 cut(s) 570
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AseI ATTAAT 1 cut(s) 492
AspLEI GCGC 2 cut(s) 330, 408
AsuHPI GGTGA 3 cut(s) 55, 214, 577
BaeGI GKGCMC 1 cut(s) 669
BaeI ACNNNNGTAYC 4 cut(s) 12, 27, 45, 60
BanII GRGCYC 1 cut(s) 312
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 1 cut(s) 582
BfaI CTAG 1 cut(s) 89
BfmI CTRYAG 2 cut(s) 300, 358
BisI GCNGC 1 cut(s) 571
BlsI GCNGC 1 cut(s) 572
BmcAI AGTACT 2 cut(s) 578, 604
BpmI CTGGAG 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 610
Bse3DI GCAATG 1 cut(s) 202
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 2 cut(s) 323, 634
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 610
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 1 cut(s) 582
Bsh1236I CGCG 2 cut(s) 120, 328
BsiHKAI GWGCWC 1 cut(s) 669
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 671
Bsp1286I GDGCHC 2 cut(s) 312, 669
Bsp143I GATC 1 cut(s) 463
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 4 cut(s) 158, 293, 326, 585
BspFNI CGCG 2 cut(s) 120, 328
BspMAI CTGCAG 2 cut(s) 304, 362
BspPI GGATC 1 cut(s) 471
BsrDI GCAATG 1 cut(s) 202
BsrI ACTGG 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 1 cut(s) 463
BssNAI GTATAC 1 cut(s) 266
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 312
Bst1107I GTATAC 1 cut(s) 266
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 5 cut(s) 108, 423, 509, 645, 664
Bst6I CTCTTC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 686, 690
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 323, 634
BstFNI CGCG 2 cut(s) 120, 328
BstHHI GCGC 2 cut(s) 330, 408
BstKTI GATC 1 cut(s) 466
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 2 cut(s) 299, 673
BstNSI RCATGY 1 cut(s) 692
BstSFI CTRYAG 2 cut(s) 300, 358
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 2 cut(s) 120, 328
BstV1I GCAGC 1 cut(s) 582
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BstZ17I GTATAC 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 323, 634
BtsI GCAGTG 2 cut(s) 208, 367
BtsIMutI CAGTG 3 cut(s) 208, 367, 390
Cac8I GCNNGC 2 cut(s) 686, 690
CfoI GCGC 2 cut(s) 330, 408
CseI GACGC 1 cut(s) 482
Csp6I GTAC 4 cut(s) 21, 577, 603, 619
CviAII CATG 4 cut(s) 313, 552, 582, 689
CviJI RGCY 5 cut(s) 88, 143, 310, 380, 684
CviKI_1 RGCY 5 cut(s) 88, 143, 310, 380, 684
CviQI GTAC 4 cut(s) 21, 577, 603, 619
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 4 cut(s) 316, 555, 585, 692
FaqI GGGAC 1 cut(s) 671
FatI CATG 4 cut(s) 312, 551, 581, 688
FauI CCCGC 1 cut(s) 151
FauNDI CATATG 2 cut(s) 180, 626
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 571
FokI GGATG 2 cut(s) 330, 621
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 1 cut(s) 571
FspBI CTAG 1 cut(s) 89
GlaI GCGC 2 cut(s) 329, 407
GluI GCNGC 1 cut(s) 571
GsuI CTGGAG 1 cut(s) 627
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 330, 408
Hin1II CATG 4 cut(s) 316, 555, 585, 692
Hin6I GCGC 2 cut(s) 328, 406
HinP1I GCGC 2 cut(s) 328, 406
HinfI GANTC 2 cut(s) 365, 434
HphI GGTGA 3 cut(s) 55, 214, 577
Hpy166II GTNNAC 4 cut(s) 222, 266, 595, 667
Hpy188I TCNGA 1 cut(s) 354
Hpy188III TCNNGA 3 cut(s) 529, 638, 735
Hpy8I GTNNAC 4 cut(s) 222, 266, 595, 667
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 5 cut(s) 108, 423, 509, 645, 664
HpyCH4IV ACGT 4 cut(s) 38, 268, 414, 524
HpyCH4V TGCA 7 cut(s) 11, 302, 360, 449, 488, 630, 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 299, 673
HpySE526I ACGT 4 cut(s) 38, 268, 414, 524
Hsp92II CATG 4 cut(s) 316, 555, 585, 692
HspAI GCGC 2 cut(s) 328, 406
Kzo9I GATC 1 cut(s) 463
LmnI GCTCC 1 cut(s) 608
LpnPI CCDG 4 cut(s) 177, 542, 591, 670
Lsp1109I GCAGC 1 cut(s) 582
MaeI CTAG 1 cut(s) 89
MaeII ACGT 4 cut(s) 38, 268, 414, 524
MaeIII GTNAC 2 cut(s) 43, 295
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 2 cut(s) 705, 710
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 312, 669
MluCI AATT 3 cut(s) 126, 442, 489
MlyI GAGTC 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 106
MnlI CCTC 5 cut(s) 11, 199, 252, 682, 712
MseI TTAA 6 cut(s) 129, 186, 237, 332, 492, 545
MvnI CGCG 2 cut(s) 120, 328
MwoI GCNNNNNNNGC 2 cut(s) 299, 673
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 2 cut(s) 180, 626
NdeII GATC 1 cut(s) 463
NlaIII CATG 4 cut(s) 316, 555, 585, 692
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 692
PaeI GCATGC 1 cut(s) 692
PfeI GAWTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 415
PkrI GCNGC 1 cut(s) 572
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
Ppu21I YACGTR 1 cut(s) 269
PshBI ATTAAT 1 cut(s) 492
PsrI GAACNNNNNNTAC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 304, 362
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 4 cut(s) 22, 578, 604, 620
RsaNI GTAC 4 cut(s) 21, 577, 603, 619
SaqAI TTAA 6 cut(s) 129, 186, 237, 332, 492, 545
SatI GCNGC 1 cut(s) 571
Sau3AI GATC 1 cut(s) 463
ScaI AGTACT 2 cut(s) 578, 604
SchI GAGTC 1 cut(s) 443
SduI GDGCHC 2 cut(s) 312, 669
SetI ASST 8 cut(s) 22, 41, 90, 126, 145, 271, 417, 527
SfcI CTRYAG 2 cut(s) 300, 358
SphI GCATGC 1 cut(s) 692
Sse9I AATT 3 cut(s) 126, 442, 489
SsiI CCGC 4 cut(s) 158, 293, 326, 585
SspMI CTAG 1 cut(s) 89
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 5 cut(s) 108, 423, 509, 645, 664
TaiI ACGT 4 cut(s) 41, 271, 417, 527
TaqI TCGA 2 cut(s) 33, 72
TasI AATT 3 cut(s) 126, 442, 489
TatI WGTACW 3 cut(s) 576, 602, 618
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 6 cut(s) 129, 186, 237, 332, 492, 545
Tru9I TTAA 6 cut(s) 129, 186, 237, 332, 492, 545
TscAI CASTG 3 cut(s) 208, 367, 390
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 570
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 3 cut(s) 17, 552, 710
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 3 cut(s) 208, 367, 390
Van91I CCANNNNNTGG 1 cut(s) 415
VneI GTGCAC 1 cut(s) 665
VspI ATTAAT 1 cut(s) 492
XceI RCATGY 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 1 cut(s) 89
ZrmI AGTACT 2 cut(s) 578, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.