FvH4_7g05772

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001140
Physical Location & Seq
Forward (+)
6003013 .. 6004922
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05772.t1

Sequence Viewer

Length: 888 bp
ATGTCAACAACTGACTTCTCCGCGTGTCAGAATCTGACGGTGAAGAACCCGGCTTCCTTTTTGCGTATAAGATCTCCAGTACCTTACAAATGGAGAAACCGTGTGAAGAAATCGAAACTCGGGGACGGGATGAACTTTGCATATGGAGGAACTGGTGTTTTCGAGACACTGGTCAGTGCACCAAACATGACTACCCAAATTGATTTTCTCCAACAACTAGTCGAAGAAAAATTGTATACCAAAAGTGACTTGGACTTGTCCATCGCTTTAGTATCAGTAGCTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCACAGTCAGGATTTGCCAGCGGTGTCGACATCGATTGTTAACCAGCTTGTGGTGAACATAAAACGTATAAATGATTTGGGAGTGAAAAAAATATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGTAACTTCCATAGTTTCTTCGTATCAGAATTGCAGTGAAACTATGAACGCAGGTTCAAGATTCCACAACCAGATTTTGTACAAAAAGGTTGAAGAGTTAAAGAAGAATCAGACAACTAAGGCATCTACCATTGTAATCTTGGATCTCTATAACGCATTTTTATCTGCAATTCAACAACAAAAACACCTGGAACAAGATGCAGGAAATTCGACAACACGAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAGAAGTGGAGCAAACAAGTACACAGTGTGCAGTAATCCCGGGAGGTCTTTCTTTTGGGACCAGGAGCACCCTTCGCAGAATGGCTGGCGTGCTGTTTATTCTGCAATCAAGTCTTCTCTACTTCCATTGTCCATTAATATCAATAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

296

Amino Acids

32.57

Weight (kDa)

8.74

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 280 3e-14 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 367
AccI GTMKAC 2 cut(s) 236, 344
AccII CGCG 1 cut(s) 23
AciI CCGC 2 cut(s) 21, 338
AclWI GGATC 1 cut(s) 600
AcsI RAATTY 1 cut(s) 655
AdeI CACNNNGTG 1 cut(s) 762
AfaI GTAC 3 cut(s) 81, 530, 755
AfiI CCNNNNNNNGG 2 cut(s) 325, 367
AgsI TTSAA 3 cut(s) 507, 542, 623
AhlI ACTAGT 1 cut(s) 217
AjnI CCWGG 2 cut(s) 636, 795
AjuI GAANNNNNNNTTGG 4 cut(s) 189, 221, 395, 427
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 2 cut(s) 281, 364
AluI AGCT 2 cut(s) 281, 364
Alw21I GWGCWC 2 cut(s) 181, 804
Alw26I GTCTC 1 cut(s) 158
Alw44I GTGCAC 1 cut(s) 177
AlwI GGATC 1 cut(s) 600
AlwNI CAGNNNCTG 2 cut(s) 34, 281
Ama87I CYCGRG 2 cut(s) 119, 773
ApaLI GTGCAC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 296, 705
ApoI RAATTY 1 cut(s) 655
AseI ATTAAT 1 cut(s) 870
AspS9I GGNCC 1 cut(s) 793
AsuC2I CCSGG 3 cut(s) 50, 774, 775
AsuHPI GGTGA 3 cut(s) 52, 382, 712
AvaI CYCGRG 2 cut(s) 119, 773
AvaII GGWCC 1 cut(s) 793
BaeGI GKGCMC 1 cut(s) 181
BanII GRGCYC 1 cut(s) 432
BauI CACGAG 2 cut(s) 666, 715
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 2 cut(s) 181, 804
BbvI GCAGC 2 cut(s) 283, 717
BccI CCATC 1 cut(s) 269
BceAI ACGGC 1 cut(s) 434
BcgI CGANNNNNNTGC 4 cut(s) 278, 312, 639, 673
BciT130I CCWGG 2 cut(s) 638, 797
BcnI CCSGG 3 cut(s) 50, 774, 775
BcoDI GTCTC 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 217
BfaI CTAG 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 491
BglII AGATCT 1 cut(s) 71
BisI GCNGC 2 cut(s) 297, 706
BlsI GCNGC 2 cut(s) 298, 707
Bme1390I CCNGG 5 cut(s) 50, 638, 774, 775, 797
Bme18I GGWCC 1 cut(s) 793
BmeT110I CYCGRG 2 cut(s) 119, 773
BmgT120I GGNCC 1 cut(s) 793
BmiI GGNNCC 1 cut(s) 794
BmrFI CCNGG 5 cut(s) 50, 638, 774, 775, 797
BmsI GCATC 3 cut(s) 581, 637, 672
BoxI GACNNNNGTC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 60
BpuMI CCSGG 3 cut(s) 50, 774, 775
Bsa29I ATCGAT 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 432, 773
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 367
Bse1I ACTGG 3 cut(s) 77, 157, 174
BseBI CCWGG 2 cut(s) 638, 797
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 2 cut(s) 432, 773
BseGI GGATG 2 cut(s) 135, 443
BseLI CCNNNNNNNGG 2 cut(s) 325, 367
BseNI ACTGG 3 cut(s) 77, 157, 174
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 2 cut(s) 283, 717
BsgI GTGCAG 1 cut(s) 784
Bsh1236I CGCG 1 cut(s) 23
BshVI ATCGAT 1 cut(s) 350
BsiHKAI GWGCWC 2 cut(s) 181, 804
BsiHKCI CYCGRG 2 cut(s) 119, 773
BsiSI CCGG 2 cut(s) 50, 774
BslFI GGGAC 2 cut(s) 137, 806
BslI CCNNNNNNNGG 2 cut(s) 325, 367
BsmAI GTCTC 1 cut(s) 158
BsmFI GGGAC 2 cut(s) 137, 806
BsoBI CYCGRG 2 cut(s) 119, 773
Bsp1286I GDGCHC 3 cut(s) 181, 432, 804
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 2 cut(s) 71, 592
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 2 cut(s) 21, 338
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 1 cut(s) 794
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 1 cut(s) 600
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 3 cut(s) 77, 157, 174
BssECI CCNNGG 2 cut(s) 432, 773
BssMI GATC 2 cut(s) 71, 592
BssNAI GTATAC 1 cut(s) 237
BssSI CACGAG 2 cut(s) 666, 715
BssT1I CCWWGG 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 237
Bst2BI CACGAG 2 cut(s) 666, 715
Bst2UI CCWGG 2 cut(s) 638, 797
Bst4CI ACNGT 4 cut(s) 40, 101, 323, 760
Bst6I CTCTTC 1 cut(s) 537
BstAUI TGTACA 1 cut(s) 528
BstC8I GCNNGC 4 cut(s) 336, 447, 821, 825
BstDEI CTNAG 1 cut(s) 567
BstDSI CCRYGG 1 cut(s) 432
BstF5I GGATG 2 cut(s) 135, 443
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 2 cut(s) 74, 595
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 2 cut(s) 71, 592
BstMWI GCNNNNNNNGC 1 cut(s) 808
BstNI CCWGG 2 cut(s) 638, 797
BstPAI GACNNNNGTC 1 cut(s) 170
BstSCI CCNGG 5 cut(s) 48, 636, 772, 773, 795
BstSLI GKGCMC 1 cut(s) 181
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 2 cut(s) 283, 717
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 2 cut(s) 71, 592
BstYI RGATCY 2 cut(s) 71, 592
BstZ17I GTATAC 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 350
BsuTUI ATCGAT 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 135, 443
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 5 cut(s) 167, 181, 490, 677, 765
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 4 cut(s) 336, 447, 821, 825
CaiI CAGNNNCTG 2 cut(s) 34, 281
Cfr13I GGNCC 1 cut(s) 793
Cfr9I CCCGGG 1 cut(s) 773
ClaI ATCGAT 1 cut(s) 350
Csp6I GTAC 3 cut(s) 80, 529, 754
CviAII CATG 3 cut(s) 187, 433, 687
CviJI RGCY 6 cut(s) 53, 281, 364, 430, 449, 819
CviKI_1 RGCY 6 cut(s) 53, 281, 364, 430, 449, 819
CviQI GTAC 3 cut(s) 80, 529, 754
DdeI CTNAG 1 cut(s) 567
DpnI GATC 2 cut(s) 73, 594
DpnII GATC 2 cut(s) 71, 592
DraIII CACNNNGTG 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 793
Eco88I CYCGRG 2 cut(s) 119, 773
EcoRII CCWGG 2 cut(s) 636, 795
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 190, 436, 690
FaqI GGGAC 2 cut(s) 137, 806
FatI CATG 3 cut(s) 186, 432, 686
FauNDI CATATG 1 cut(s) 142
FblI GTMKAC 2 cut(s) 236, 344
Fnu4HI GCNGC 2 cut(s) 297, 706
FokI GGATG 2 cut(s) 142, 450
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 2 cut(s) 297, 706
FspBI CTAG 1 cut(s) 218
GluI GCNGC 2 cut(s) 297, 706
GsuI CTGGAG 1 cut(s) 60
HapII CCGG 2 cut(s) 50, 774
Hin1II CATG 3 cut(s) 190, 436, 690
HincII GTYRAC 3 cut(s) 6, 345, 358
HindII GTYRAC 3 cut(s) 6, 345, 358
HinfI GANTC 4 cut(s) 31, 510, 556, 673
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 2 cut(s) 50, 774
HphI GGTGA 3 cut(s) 52, 382, 712
Hpy166II GTNNAC 8 cut(s) 6, 179, 237, 345, 358, 373, 730, 756
Hpy188I TCNGA 4 cut(s) 30, 36, 477, 561
Hpy188III TCNNGA 3 cut(s) 163, 326, 507
Hpy8I GTNNAC 8 cut(s) 6, 179, 237, 345, 358, 373, 730, 756
HpyAV CCTTC 1 cut(s) 816
HpyCH4III ACNGT 4 cut(s) 40, 101, 323, 760
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 7 cut(s) 140, 179, 483, 617, 650, 765, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 808
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 3 cut(s) 190, 436, 690
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 71, 592
LmnI GCTCC 2 cut(s) 743, 799
Lsp1109I GCAGC 2 cut(s) 283, 717
LweI GCATC 3 cut(s) 581, 637, 672
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 3 cut(s) 245, 415, 451
MalI GATC 2 cut(s) 73, 594
MboI GATC 2 cut(s) 71, 592
MboII GAAGA 7 cut(s) 55, 118, 236, 459, 554, 565, 840
MflI RGATCY 2 cut(s) 71, 592
MhlI GDGCHC 3 cut(s) 181, 432, 804
MluCI AATT 5 cut(s) 198, 230, 478, 618, 655
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 2 cut(s) 140, 771
MseI TTAA 4 cut(s) 309, 357, 548, 870
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 2 cut(s) 50, 774
MspR9I CCNGG 5 cut(s) 50, 638, 774, 775, 797
MvaI CCWGG 2 cut(s) 638, 797
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 1 cut(s) 808
NciI CCSGG 3 cut(s) 50, 774, 775
NcoI CCATGG 1 cut(s) 432
NdeI CATATG 1 cut(s) 142
NdeII GATC 2 cut(s) 71, 592
NlaIII CATG 3 cut(s) 190, 436, 690
NlaIV GGNNCC 1 cut(s) 794
NmuCI GTSAC 1 cut(s) 245
PfeI GAWTC 4 cut(s) 31, 510, 556, 673
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 2 cut(s) 298, 707
PshAI GACNNNNGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 870
Psp6I CCWGG 2 cut(s) 636, 795
PspGI CCWGG 2 cut(s) 636, 795
PspN4I GGNNCC 1 cut(s) 794
PspPI GGNCC 1 cut(s) 793
PstNI CAGNNNCTG 2 cut(s) 34, 281
PsuI RGATCY 2 cut(s) 71, 592
RsaI GTAC 3 cut(s) 81, 530, 755
RsaNI GTAC 3 cut(s) 80, 529, 754
SalI GTCGAC 1 cut(s) 343
SaqAI TTAA 4 cut(s) 309, 357, 548, 870
SatI GCNGC 2 cut(s) 297, 706
Sau3AI GATC 2 cut(s) 71, 592
Sau96I GGNCC 1 cut(s) 793
ScrFI CCNGG 5 cut(s) 50, 638, 774, 775, 797
SduI GDGCHC 3 cut(s) 181, 432, 804
SetI ASST 8 cut(s) 85, 283, 366, 385, 505, 540, 639, 782
SfaNI GCATC 3 cut(s) 581, 637, 672
SinI GGWCC 1 cut(s) 793
SmaI CCCGGG 1 cut(s) 775
SpeI ACTAGT 1 cut(s) 217
Sse9I AATT 5 cut(s) 198, 230, 478, 618, 655
SsiI CCGC 2 cut(s) 21, 338
SspI AATATT 1 cut(s) 411
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 5 cut(s) 48, 636, 772, 773, 795
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 4 cut(s) 40, 101, 323, 760
TaiI ACGT 1 cut(s) 385
TaqI TCGA 6 cut(s) 113, 162, 222, 344, 350, 659
TasI AATT 5 cut(s) 198, 230, 478, 618, 655
TatI WGTACW 2 cut(s) 528, 753
TfiI GAWTC 4 cut(s) 31, 510, 556, 673
Tru1I TTAA 4 cut(s) 309, 357, 548, 870
Tru9I TTAA 4 cut(s) 309, 357, 548, 870
TscAI CASTG 5 cut(s) 174, 181, 490, 684, 765
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 296, 705
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 146, 509
TspMI CCCGGG 1 cut(s) 773
TspRI CASTG 5 cut(s) 174, 181, 490, 684, 765
Van91I CCANNNNNTGG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 793
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 1 cut(s) 655
XcmI CCANNNNNNNNNTGG 2 cut(s) 247, 586
XmaI CCCGGG 1 cut(s) 773
XmiI GTMKAC 2 cut(s) 236, 344
XspI CTAG 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.