Rroxscaffold_4G00317950

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001140
Physical Location & Seq
Reverse (-)
43601658 .. 43605585
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317950.1

Sequence Viewer

Length: 1092 bp
ATGGAGCAAACCACCATCGTCTCATTCTGTCTCTTTCTTCTCTACATCTCCACTTGTCCACAAGTACTTCATTCAGCCATAACCTCCGACCACCGCCACCGCCACCGCCACCAACGCCATGACAGGAGATCTGTAAAGCTTTTTGTTTTCGGGGATTCGTATGCTGATACTGGGAACAGCGGCAAATCTGCAACTGGTTCGTGGAAGCCGCCTTATGGGATCACTTTTCCCGGAAAGCCGGCAGGCCGCTTCTCCGATGGTCGCGTGCTCACTGATTATATAGCTTCCTTTTTGGGTATAAGATCTCTAGTACCTTACGAATTGAGAAACATTGAAGAGAAATCGAAACTAAGGAACGGGATGAACTTTGCATATGGAGGAACTGGTGTTTTTGAGACTTTGGTGAGTGCACCAAACATGACTACCCAAATCGACTTTCTCCAACAACTAGTCGAAGAAAAATTGTATACCAAAAGTGGCTTGGACTCGTCCGTTGCTTTAGTATCACTAGGTGGAAACGACTATGCTGCTTATTTTCTTAAAGGAAACCAGAGTCAGGATTTTTCAGCGGTGTCCACATCGATTGTCAACCAGCTTGTGGTGAACATAAAGCGTATAAATGATTTGGGAGTGCGAAAATTATTGGTTACAGCAATAGAGCCCATGGGATGTTTGCCAGCCGTAACTTCCATTTTTTCTTCGTATCAGAATTGCAGTGAAAGTATGAATGCAGGTTCAAGATTCCACAACCAGATTTTGCACCAAAAGGTTGAAGATTTAAAGAATCAGACAACTACGATATCAACATTTGTAATCTTGGATCTCTATAATGCATTTCTGTCCGCAATCCAACAAAAAAAACACCTGCAACAAGGAAATTCGACAAGATCAGAGAATCCACTGATGCCATGCTGTGTTGGGGTGAGCAGCGAATACTCGTGTGGGAGTGTGGACGAGAAAAGTGGAGCAAACAAGTACAGAGTGTGCAGTAATCCGGAAAGGTCTTTCTTTTGGGACATGGAGCACCTTTCGCAGAATGGCTGGCATGCTGTTTATTCTGCAATCAAGTCTTCTCTTCGTCCATTGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

363

Amino Acids

40.44

Weight (kDa)

8.91

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 354 1.3e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 873
Acc36I ACCTGC 2 cut(s) 722, 873
AccB7I CCANNNNNTGG 1 cut(s) 598
AccI GTMKAC 1 cut(s) 467
AccII CGCG 1 cut(s) 264
AccIII TCCGGA 1 cut(s) 994
AciI CCGC 8 cut(s) 94, 100, 106, 180, 209, 247, 569, 843
AclWI GGATC 2 cut(s) 227, 828
AcsI RAATTY 1 cut(s) 877
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 3 cut(s) 66, 312, 977
AfiI CCNNNNNNNGG 3 cut(s) 215, 556, 598
AgsI TTSAA 3 cut(s) 335, 738, 773
AhlI ACTAGT 1 cut(s) 448
AjuI GAANNNNNNNTTGG 2 cut(s) 420, 452
AluBI AGCT 3 cut(s) 139, 284, 595
AluI AGCT 3 cut(s) 139, 284, 595
Alw21I GWGCWC 3 cut(s) 270, 412, 1026
Alw26I GTCTC 3 cut(s) 25, 35, 389
Alw44I GTGCAC 1 cut(s) 408
AlwI GGATC 2 cut(s) 227, 828
Aor13HI TCCGGA 1 cut(s) 994
AoxI GGCC 1 cut(s) 244
ApaLI GTGCAC 1 cut(s) 408
ApeKI GCWGC 2 cut(s) 527, 927
ApoI RAATTY 1 cut(s) 877
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 3 cut(s) 415, 613, 934
BaeGI GKGCMC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 663
BauI CACGAG 1 cut(s) 937
BbsI GAAGAC 1 cut(s) 1062
Bbv12I GWGCWC 3 cut(s) 270, 412, 1026
BbvI GCAGC 2 cut(s) 514, 939
BccI CCATC 2 cut(s) 23, 251
BceAI ACGGC 1 cut(s) 665
BcgI CGANNNNNNTGC 4 cut(s) 180, 214, 509, 543
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 3 cut(s) 25, 35, 389
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 3 cut(s) 308, 449, 509
BfuAI ACCTGC 2 cut(s) 722, 873
BglII AGATCT 2 cut(s) 128, 302
BisI GCNGC 5 cut(s) 181, 209, 247, 528, 928
BlsI GCNGC 5 cut(s) 182, 210, 248, 529, 929
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 231
BmrI ACTGGG 1 cut(s) 180
BmsI GCATC 1 cut(s) 894
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 1062
BpuMI CCSGG 1 cut(s) 231
Bsa29I ATCGAT 1 cut(s) 581
BsaJI CCNNGG 1 cut(s) 663
BsaWI WCCGGW 1 cut(s) 994
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 556, 598
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 3 cut(s) 175, 199, 388
Bse3DI GCAATG 1 cut(s) 1082
BseAI TCCGGA 1 cut(s) 994
BseCI ATCGAT 1 cut(s) 581
BseDI CCNNGG 1 cut(s) 663
BseGI GGATG 2 cut(s) 366, 674
BseLI CCNNNNNNNGG 3 cut(s) 215, 556, 598
BseMI GCAATG 1 cut(s) 1082
BseNI ACTGG 3 cut(s) 175, 199, 388
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 2 cut(s) 514, 939
BsgI GTGCAG 1 cut(s) 1006
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 581
BsiHKAI GWGCWC 3 cut(s) 270, 412, 1026
BsiSI CCGG 3 cut(s) 231, 239, 995
BslFI GGGAC 1 cut(s) 1028
BslI CCNNNNNNNGG 3 cut(s) 215, 556, 598
BsmAI GTCTC 3 cut(s) 25, 35, 389
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 1028
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 1 cut(s) 246
Bsp1286I GDGCHC 4 cut(s) 270, 412, 663, 1026
Bsp13I TCCGGA 1 cut(s) 994
Bsp143I GATC 5 cut(s) 128, 219, 302, 820, 887
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 8 cut(s) 94, 100, 106, 180, 209, 247, 569, 843
BspANI GGCC 1 cut(s) 246
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 1 cut(s) 994
BspFNI CGCG 1 cut(s) 264
BspMI ACCTGC 2 cut(s) 722, 873
BspPI GGATC 2 cut(s) 227, 828
BsrDI GCAATG 1 cut(s) 1082
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 3 cut(s) 175, 199, 388
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 663
BssMI GATC 5 cut(s) 128, 219, 302, 820, 887
BssNAI GTATAC 1 cut(s) 468
BssSI CACGAG 1 cut(s) 937
BssT1I CCWWGG 1 cut(s) 663
Bst1107I GTATAC 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 937
Bst6I CTCTTC 2 cut(s) 330, 1080
BstC8I GCNNGC 6 cut(s) 240, 244, 266, 678, 1043, 1047
BstDEI CTNAG 1 cut(s) 350
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 366, 674
BstFNI CGCG 1 cut(s) 264
BstKTI GATC 5 cut(s) 131, 222, 305, 823, 890
BstMAI GTCTC 3 cut(s) 25, 35, 389
BstMBI GATC 5 cut(s) 128, 219, 302, 820, 887
BstMWI GCNNNNNNNGC 2 cut(s) 114, 1030
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 412
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 514, 939
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 3 cut(s) 128, 302, 820
BstYI RGATCY 3 cut(s) 128, 302, 820
BstZ17I GTATAC 1 cut(s) 468
Bsu15I ATCGAT 1 cut(s) 581
BsuRI GGCC 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 581
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 2 cut(s) 366, 674
BtsI GCAGTG 1 cut(s) 721
BtsIMutI CAGTG 3 cut(s) 270, 721, 899
BveI ACCTGC 2 cut(s) 722, 873
Cac8I GCNNGC 6 cut(s) 240, 244, 266, 678, 1043, 1047
Cfr10I RCCGGY 1 cut(s) 238
ClaI ATCGAT 1 cut(s) 581
Csp6I GTAC 3 cut(s) 65, 311, 976
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 6 cut(s) 119, 418, 664, 909, 1018, 1046
CviQI GTAC 3 cut(s) 65, 311, 976
DdeI CTNAG 1 cut(s) 350
DpnI GATC 5 cut(s) 130, 221, 304, 822, 889
DpnII GATC 5 cut(s) 128, 219, 302, 820, 887
DraI TTTAAA 1 cut(s) 780
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 2 cut(s) 330, 1080
EarI CTCTTC 2 cut(s) 330, 1080
Eco130I CCWWGG 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 663
Eco32I GATATC 1 cut(s) 801
EcoRV GATATC 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 663
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 6 cut(s) 122, 421, 667, 912, 1021, 1049
FaqI GGGAC 1 cut(s) 1028
FatI CATG 6 cut(s) 118, 417, 663, 908, 1017, 1045
FauNDI CATATG 1 cut(s) 373
FblI GTMKAC 1 cut(s) 467
Fnu4HI GCNGC 5 cut(s) 181, 209, 247, 528, 928
FokI GGATG 2 cut(s) 373, 681
FriOI GRGCYC 1 cut(s) 663
Fsp4HI GCNGC 5 cut(s) 181, 209, 247, 528, 928
FspBI CTAG 3 cut(s) 308, 449, 509
GluI GCNGC 5 cut(s) 181, 209, 247, 528, 928
HaeIII GGCC 1 cut(s) 246
HapII CCGG 3 cut(s) 231, 239, 995
Hin1II CATG 6 cut(s) 122, 421, 667, 912, 1021, 1049
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 6 cut(s) 155, 485, 553, 741, 784, 895
HpaII CCGG 3 cut(s) 231, 239, 995
HphI GGTGA 3 cut(s) 415, 613, 934
Hpy166II GTNNAC 7 cut(s) 59, 410, 468, 576, 589, 604, 952
Hpy188I TCNGA 5 cut(s) 88, 256, 708, 789, 892
Hpy188III TCNNGA 3 cut(s) 557, 738, 995
Hpy8I GTNNAC 7 cut(s) 59, 410, 468, 576, 589, 604, 952
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 1030
HpyF3I CTNAG 1 cut(s) 350
Hsp92II CATG 6 cut(s) 122, 421, 667, 912, 1021, 1049
Kpn2I TCCGGA 1 cut(s) 994
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 5 cut(s) 128, 219, 302, 820, 887
LmnI GCTCC 3 cut(s) 4, 965, 1021
Lsp1109I GCAGC 2 cut(s) 514, 939
LweI GCATC 1 cut(s) 894
MaeI CTAG 3 cut(s) 308, 449, 509
MaeIII GTNAC 2 cut(s) 646, 682
MalI GATC 5 cut(s) 130, 221, 304, 822, 889
MboI GATC 5 cut(s) 128, 219, 302, 820, 887
MboII GAAGA 7 cut(s) 29, 347, 467, 690, 785, 1062, 1067
MflI RGATCY 3 cut(s) 128, 302, 820
MhlI GDGCHC 4 cut(s) 270, 412, 663, 1026
MluCI AATT 5 cut(s) 320, 461, 638, 709, 877
MlyI GAGTC 2 cut(s) 479, 562
MmeI TCCRAC 3 cut(s) 111, 466, 874
MnlI CCTC 2 cut(s) 94, 371
Mph1103I ATGCAT 1 cut(s) 835
MroI TCCGGA 1 cut(s) 994
MroNI GCCGGC 1 cut(s) 238
MseI TTAA 3 cut(s) 540, 779, 1090
MspA1I CMGCKG 2 cut(s) 180, 569
MspI CCGG 3 cut(s) 231, 239, 995
MspR9I CCNGG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 733
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 114, 1030
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 1 cut(s) 231
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 373
NdeII GATC 5 cut(s) 128, 219, 302, 820, 887
NgoMIV GCCGGC 1 cut(s) 238
NlaIII CATG 6 cut(s) 122, 421, 667, 912, 1021, 1049
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 1 cut(s) 1049
PaeI GCATGC 1 cut(s) 1049
PaqCI CACCTGC 1 cut(s) 873
PcsI WCGNNNNNNNCGW 1 cut(s) 1084
PctI GAATGC 1 cut(s) 733
PdiI GCCGGC 1 cut(s) 240
PfeI GAWTC 4 cut(s) 155, 741, 784, 895
PflMI CCANNNNNTGG 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 229
PkrI GCNGC 5 cut(s) 182, 210, 248, 529, 929
PleI GAGTC 2 cut(s) 479, 561
PpsI GAGTC 2 cut(s) 479, 561
PsuI RGATCY 3 cut(s) 128, 302, 820
RsaI GTAC 3 cut(s) 66, 312, 977
RsaNI GTAC 3 cut(s) 65, 311, 976
SaqAI TTAA 3 cut(s) 540, 779, 1090
SatI GCNGC 5 cut(s) 181, 209, 247, 528, 928
Sau3AI GATC 5 cut(s) 128, 219, 302, 820, 887
ScaI AGTACT 1 cut(s) 66
SchI GAGTC 2 cut(s) 479, 562
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 4 cut(s) 270, 412, 663, 1026
SfaNI GCATC 1 cut(s) 894
SpeI ACTAGT 1 cut(s) 448
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 5 cut(s) 320, 461, 638, 709, 877
SsiI CCGC 8 cut(s) 94, 100, 106, 180, 209, 247, 569, 843
SspMI CTAG 3 cut(s) 308, 449, 509
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 663
TaqI TCGA 5 cut(s) 344, 432, 453, 581, 881
TasI AATT 5 cut(s) 320, 461, 638, 709, 877
TatI WGTACW 2 cut(s) 64, 975
TauI GCSGC 3 cut(s) 183, 211, 249
TfiI GAWTC 4 cut(s) 155, 741, 784, 895
Tru1I TTAA 3 cut(s) 540, 779, 1090
Tru9I TTAA 3 cut(s) 540, 779, 1090
TscAI CASTG 3 cut(s) 277, 721, 906
TseI GCWGC 2 cut(s) 527, 927
TspDTI ATGAA 3 cut(s) 59, 377, 740
TspGWI ACGGA 1 cut(s) 481
TspRI CASTG 3 cut(s) 277, 721, 906
Van91I CCANNNNNTGG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 408
XapI RAATTY 1 cut(s) 877
XceI RCATGY 1 cut(s) 1049
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 467
XspI CTAG 3 cut(s) 308, 449, 509
ZrmI AGTACT 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.