FvH4_7g05761

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001140
Physical Location & Seq
Forward (+)
5998492 .. 5998836
345 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g05761.t1

Sequence Viewer

Length: 330 bp
ATGGAGCAAACCACCATCCTCTCATTCTGTCTCTTTCTTCTCTACATCTCCACTTGTCCCCAAGTACTTCATTCAGCCATAACCTCCGGCCACCGCCACCGCCACCACGAGAAGAGGTCTGTAAAGCTTTTTGTTTTCAGGGATTCTTATGCCGATACTGGGAATAGCGGCAAGTTTGCCACTGGTTCATGGGTAGCGCCTTATGGAATCACTTTTCCCGGAAAGCCGGCCGGCCGCTTCTCCGATGGTCGGGTGCTCACTGATTATATAGGTTCCTTCATTTTCTTCTTTAATTCTCCCTCATCTTTTTCACTTGCTAATCACTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

110

Amino Acids

12.17

Weight (kDa)

9.39

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 94, 100, 168, 235
AcoI YGGCCR 3 cut(s) 88, 228, 232
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 159, 249
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 35
AoxI GGCC 3 cut(s) 88, 228, 232
AspLEI GCGC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 219
BauI CACGAG 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 258
BccI CCATC 2 cut(s) 23, 239
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 200
BisI GCNGC 2 cut(s) 169, 235
BlsI GCNGC 2 cut(s) 170, 236
BmcAI AGTACT 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 168
BpuMI CCSGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 249
Bse118I RCCGGY 2 cut(s) 226, 230
Bse1I ACTGG 2 cut(s) 163, 187
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 159, 249
BseNI ACTGG 2 cut(s) 163, 187
BseX3I CGGCCG 2 cut(s) 228, 232
Bsh1285I CGRYCG 2 cut(s) 231, 235
BshFI GGCC 3 cut(s) 90, 230, 234
BsiEI CGRYCG 2 cut(s) 231, 235
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 4 cut(s) 87, 219, 227, 231
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 2 cut(s) 159, 249
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 3 cut(s) 90, 230, 234
Bsp1286I GDGCHC 1 cut(s) 258
BspACI CCGC 4 cut(s) 94, 100, 168, 235
BspANI GGCC 3 cut(s) 90, 230, 234
BspLI GGNNCC 1 cut(s) 274
BsrFI RCCGGY 2 cut(s) 226, 230
BsrI ACTGG 2 cut(s) 163, 187
BssAI RCCGGY 2 cut(s) 226, 230
BssSI CACGAG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 107
Bst6I CTCTTC 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 228, 232
BstF5I GGATG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 1 cut(s) 199
BstMAI GTCTC 1 cut(s) 35
BstMCI CGRYCG 2 cut(s) 231, 235
BstSCI CCNGG 1 cut(s) 217
BstZI CGGCCG 2 cut(s) 228, 232
BsuRI GGCC 3 cut(s) 90, 230, 234
BtsCI GGATG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 180, 258
Cac8I GCNNGC 2 cut(s) 228, 232
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 2 cut(s) 226, 230
Csp6I GTAC 1 cut(s) 65
CviAII CATG 1 cut(s) 189
CviJI RGCY 6 cut(s) 77, 90, 127, 226, 230, 234
CviKI_1 RGCY 6 cut(s) 77, 90, 127, 226, 230, 234
CviQI GTAC 1 cut(s) 65
EaeI YGGCCR 3 cut(s) 88, 228, 232
EagI CGGCCG 2 cut(s) 228, 232
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
EclXI CGGCCG 2 cut(s) 228, 232
Eco52I CGGCCG 2 cut(s) 228, 232
FaeI CATG 1 cut(s) 192
FaiI YATR 6 cut(s) 80, 150, 190, 204, 267, 269
FaqI GGGAC 1 cut(s) 42
FatI CATG 1 cut(s) 188
Fnu4HI GCNGC 2 cut(s) 169, 235
FokI GGATG 1 cut(s) 2
FseI GGCCGGCC 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 169, 235
GlaI GCGC 1 cut(s) 198
GluI GCNGC 2 cut(s) 169, 235
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 3 cut(s) 90, 230, 234
HapII CCGG 4 cut(s) 87, 219, 227, 231
HhaI GCGC 1 cut(s) 199
Hin1II CATG 1 cut(s) 192
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 2 cut(s) 143, 207
HpaII CCGG 4 cut(s) 87, 219, 227, 231
Hpy188I TCNGA 1 cut(s) 244
HpyAV CCTTC 1 cut(s) 286
Hsp92II CATG 1 cut(s) 192
HspAI GCGC 1 cut(s) 197
KroI GCCGGC 2 cut(s) 226, 230
KroNI GCCGGC 2 cut(s) 228, 232
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 7 cut(s) 100, 124, 144, 168, 232, 240, 244
MboII GAAGA 3 cut(s) 29, 124, 277
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 1 cut(s) 292
MnlI CCTC 4 cut(s) 29, 94, 108, 310
MroNI GCCGGC 2 cut(s) 226, 230
MseI TTAA 1 cut(s) 291
MspI CCGG 4 cut(s) 87, 219, 227, 231
MspR9I CCNGG 1 cut(s) 219
NaeI GCCGGC 2 cut(s) 228, 232
NciI CCSGG 1 cut(s) 219
NgoMIV GCCGGC 2 cut(s) 226, 230
NlaIII CATG 1 cut(s) 192
NlaIV GGNNCC 1 cut(s) 274
PdiI GCCGGC 2 cut(s) 228, 232
PfeI GAWTC 2 cut(s) 143, 207
PfoI TCCNGGA 1 cut(s) 217
PkrI GCNGC 2 cut(s) 170, 236
PspN4I GGNNCC 1 cut(s) 274
RigI GGCCGGCC 1 cut(s) 234
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 2 cut(s) 169, 235
ScaI AGTACT 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 1 cut(s) 258
SetI ASST 4 cut(s) 86, 119, 129, 274
Sse9I AATT 1 cut(s) 292
SsiI CCGC 4 cut(s) 94, 100, 168, 235
StyD4I CCNGG 1 cut(s) 217
TasI AATT 1 cut(s) 292
TatI WGTACW 1 cut(s) 64
TauI GCSGC 2 cut(s) 171, 237
TfiI GAWTC 2 cut(s) 143, 207
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 2 cut(s) 187, 265
TspDTI ATGAA 3 cut(s) 59, 177, 268
TspRI CASTG 2 cut(s) 187, 265
ZrmI AGTACT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.