Rw0G004970

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00191
OG0001140
Physical Location & Seq
Reverse (-)
46986 .. 55171
8186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004970.1

Sequence Viewer

Length: 1086 bp
ATGTCTACAGATTCTTTGACTAATATTTTTCTTCATTTAATTAAATTCTTTCCATCAATTTTTCTTTCCAAATGTGCGCTACTGGTCTCAGCTGGTCATCACCATGGTCACCATGGCCATCACCACCATCATACACACCGATACTTGTTCGATTGGCGTCCAACGAAACTGTTTGTTTTCGGAGACTCGTACTCCGACACAGGCAACAACATAAAGTCTGTGACCAAGTCTTGGAAATATCCTTACGGAATCACCTTCCCGGGAAAACCCACCGGCCGTTTCTCCGATGGCCGCGTCCTCACTGATTTCCTTGCTAAGTTTGTAGGGGTGAAGTCTCCAATACCATACAAATTTAGAAATGTCGGAGTTAAACATTTGAAATACGGAATGAATTTCGCATATGGAGGCACCGGTGTGTTCAATACCTTGGTTGCGGCACCAAACATGACCACCCAAATCGATTTTTTTCAGCAATTCATCAAAGACTCCGTTTTCAATGCCCAAGACCTACACTCCTCCGTGGCATTGATCACTCTTGCTGGCAATGACTACGCATATTACCTGGCCCTGAATAATTCTTCTGAGGGTTTTAAACCTTTCATCACATCAGTTGTAAATCAATTAAGTGTGAACTTGAAACGCGTCTATGACTTGGGAGTGAAGAAAATAGTTGTTACAGCTCTACAACCTTTGGGATGTCTTCCTGGCAGCACAGCCGAATTCTCATTCCAGAAGTGCAATGGAACACAAAACTTGCTGGTCGGTTTCCACAACCTCTTGTTGCAGCAAGCTATAGCCAAGTTGAACAATGAATCGAAGAGTTCTTTCATCGTTCTCGATCTATATACCTCATTCATGTCCGTGCTGAAGAATAAAGGAGACCATCCAGGAAGCATAAAGTTTGAGAACCCGTTGAGGCCATGTTGTGTTGGCGATTGTGGGAGTGTCGATGAGAGTGGGGCGAAGAAGTACACCATATGTGACAACCCGAAATCTGCATTCTTTTGGGACGGTGCTCACCCTACTCAACAGGGCTGGCTTGCTGTGTATTCAGCTTTGCAAGGAACACTTGAAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

361

Amino Acids

40.14

Weight (kDa)

9.06

Isoelectric Point (pI)

23.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 57 - 350 2.6e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 407, 436
AccB7I CCANNNNNTGG 1 cut(s) 231
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 294, 642
AciI CCGC 2 cut(s) 292, 434
AcoI YGGCCR 3 cut(s) 115, 274, 289
AcsI RAATTY 4 cut(s) 44, 350, 391, 719
AcuI CTGAAG 1 cut(s) 887
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 3 cut(s) 191, 971, 1082
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 640
AgeI ACCGGT 1 cut(s) 410
AgsI TTSAA 6 cut(s) 379, 421, 496, 637, 805, 1073
AjnI CCWGG 3 cut(s) 561, 703, 886
AleI CACNNNNGTG 1 cut(s) 413
AluBI AGCT 4 cut(s) 92, 680, 791, 1055
AluI AGCT 4 cut(s) 92, 680, 791, 1055
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 4 cut(s) 91, 177, 339, 873
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 5 cut(s) 115, 274, 289, 564, 917
ApeKI GCWGC 2 cut(s) 708, 784
ApoI RAATTY 4 cut(s) 44, 350, 391, 719
AsiGI ACCGGT 1 cut(s) 410
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 565
AsuC2I CCSGG 2 cut(s) 260, 261
AsuHPI GGTGA 6 cut(s) 92, 101, 113, 244, 340, 1010
AvaI CYCGRG 1 cut(s) 259
BalI TGGCCA 1 cut(s) 117
BanI GGYRCC 2 cut(s) 407, 436
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 1018
BbvI GCAGC 2 cut(s) 720, 796
BccI CCATC 5 cut(s) 61, 126, 135, 281, 891
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 3 cut(s) 563, 705, 888
BclI TGATCA 1 cut(s) 528
BcnI CCSGG 2 cut(s) 260, 261
BcoDI GTCTC 4 cut(s) 91, 177, 339, 873
BfaI CTAG 1 cut(s) 1084
BfmI CTRYAG 2 cut(s) 6, 792
BisI GCNGC 4 cut(s) 292, 435, 709, 785
BlsI GCNGC 4 cut(s) 293, 436, 710, 786
Bme1390I CCNGG 5 cut(s) 260, 261, 563, 705, 888
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 2 cut(s) 409, 438
BmrFI CCNGG 5 cut(s) 260, 261, 563, 705, 888
BpiI GAAGAC 1 cut(s) 692
BpuMI CCSGG 2 cut(s) 260, 261
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 157
BsaI GGTCTC 2 cut(s) 91, 873
BsaJI CCNNGG 5 cut(s) 103, 112, 259, 426, 519
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 497, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse118I RCCGGY 2 cut(s) 272, 410
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 2 cut(s) 550, 745
BseBI CCWGG 3 cut(s) 563, 705, 888
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 5 cut(s) 103, 112, 259, 426, 519
BseGI GGATG 2 cut(s) 701, 883
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMI GCAATG 2 cut(s) 550, 745
BseMII CTCAG 2 cut(s) 102, 573
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 505
BseX3I CGGCCG 1 cut(s) 274
BseXI GCAGC 2 cut(s) 720, 796
Bsh1236I CGCG 2 cut(s) 294, 642
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 5 cut(s) 117, 276, 291, 566, 919
BshNI GGYRCC 2 cut(s) 407, 436
BshTI ACCGGT 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 3 cut(s) 260, 273, 411
BslFI GGGAC 1 cut(s) 1022
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 4 cut(s) 91, 177, 339, 873
BsmFI GGGAC 1 cut(s) 1022
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 5 cut(s) 117, 276, 291, 566, 919
Bso31I GGTCTC 2 cut(s) 91, 873
BsoBI CYCGRG 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp143I GATC 2 cut(s) 528, 838
Bsp19I CCATGG 2 cut(s) 103, 112
BspACI CCGC 2 cut(s) 292, 434
BspANI GGCC 5 cut(s) 117, 276, 291, 566, 919
BspCNI CTCAG 2 cut(s) 101, 574
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 2 cut(s) 294, 642
BspLI GGNNCC 2 cut(s) 409, 438
BspT107I GGYRCC 2 cut(s) 407, 436
BspTNI GGTCTC 2 cut(s) 91, 873
BsrDI GCAATG 2 cut(s) 550, 745
BsrFI RCCGGY 2 cut(s) 272, 410
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 2 cut(s) 272, 410
BssECI CCNNGG 5 cut(s) 103, 112, 259, 426, 519
BssMI GATC 2 cut(s) 528, 838
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 3 cut(s) 103, 112, 426
Bst2UI CCWGG 3 cut(s) 563, 705, 888
Bst4CI ACNGT 2 cut(s) 171, 1013
Bst6I CTCTTC 1 cut(s) 812
BstACI GRCGYC 1 cut(s) 157
BstC8I GCNNGC 4 cut(s) 541, 789, 1037, 1041
BstDEI CTNAG 3 cut(s) 88, 315, 582
BstDSI CCRYGG 3 cut(s) 103, 112, 519
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 2 cut(s) 701, 883
BstFNI CGCG 2 cut(s) 294, 642
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 531, 841
BstMAI GTCTC 4 cut(s) 91, 177, 339, 873
BstMBI GATC 2 cut(s) 528, 838
BstMCI CGRYCG 1 cut(s) 277
BstNI CCWGG 3 cut(s) 563, 705, 888
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 5 cut(s) 258, 259, 561, 703, 886
BstSFI CTRYAG 2 cut(s) 6, 792
BstUI CGCG 2 cut(s) 294, 642
BstV1I GCAGC 2 cut(s) 720, 796
BstV2I GAAGAC 1 cut(s) 692
BstZI CGGCCG 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 5 cut(s) 117, 276, 291, 566, 919
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 3 cut(s) 103, 112, 519
BtsCI GGATG 2 cut(s) 701, 883
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 4 cut(s) 541, 789, 1037, 1041
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 2 cut(s) 272, 410
Cfr13I GGNCC 1 cut(s) 565
Cfr9I CCCGGG 1 cut(s) 259
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 3 cut(s) 146, 283, 631
Csp6I GTAC 3 cut(s) 190, 970, 1081
CspAI ACCGGT 1 cut(s) 410
CviAII CATG 5 cut(s) 104, 113, 445, 856, 921
CviQI GTAC 3 cut(s) 190, 970, 1081
DdeI CTNAG 3 cut(s) 88, 315, 582
DpnI GATC 2 cut(s) 530, 840
DpnII GATC 2 cut(s) 528, 838
DraI TTTAAA 1 cut(s) 592
EaeI YGGCCR 3 cut(s) 115, 274, 289
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 812
EarI CTCTTC 1 cut(s) 812
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 3 cut(s) 103, 112, 426
Eco31I GGTCTC 2 cut(s) 91, 873
Eco52I CGGCCG 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 887
Eco88I CYCGRG 1 cut(s) 259
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 3 cut(s) 561, 703, 886
EcoT14I CCWWGG 3 cut(s) 103, 112, 426
ErhI CCWWGG 3 cut(s) 103, 112, 426
FaeI CATG 5 cut(s) 107, 116, 448, 859, 924
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 1 cut(s) 1022
FatI CATG 5 cut(s) 103, 112, 444, 855, 920
FauNDI CATATG 2 cut(s) 400, 977
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 292, 435, 709, 785
FokI GGATG 2 cut(s) 708, 870
Fsp4HI GCNGC 4 cut(s) 292, 435, 709, 785
FspBI CTAG 1 cut(s) 1084
GlaI GCGC 1 cut(s) 78
GluI GCNGC 4 cut(s) 292, 435, 709, 785
HaeIII GGCC 5 cut(s) 117, 276, 291, 566, 919
HapII CCGG 3 cut(s) 260, 273, 411
HgaI GACGC 3 cut(s) 146, 283, 631
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 107, 116, 448, 859, 924
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 5 cut(s) 11, 185, 249, 485, 812
HpaII CCGG 3 cut(s) 260, 273, 411
HphI GGTGA 6 cut(s) 92, 101, 113, 244, 340, 1010
Hpy166II GTNNAC 3 cut(s) 6, 631, 972
Hpy188I TCNGA 5 cut(s) 182, 196, 286, 365, 583
Hpy188III TCNNGA 2 cut(s) 730, 836
Hpy8I GTNNAC 3 cut(s) 6, 631, 972
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 2 cut(s) 171, 1013
HpyCH4V TGCA 4 cut(s) 738, 784, 998, 1060
HpyF3I CTNAG 3 cut(s) 88, 315, 582
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 107, 116, 448, 859, 924
HspAI GCGC 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 528, 838
Lsp1109I GCAGC 2 cut(s) 720, 796
MaeI CTAG 1 cut(s) 1084
MaeIII GTNAC 4 cut(s) 107, 220, 673, 980
MalI GATC 2 cut(s) 530, 840
MboI GATC 2 cut(s) 528, 838
MboII GAAGA 8 cut(s) 23, 570, 673, 692, 829, 880, 976, 1085
MhlI GDGCHC 1 cut(s) 1018
MlsI TGGCCA 1 cut(s) 117
MluI ACGCGT 1 cut(s) 640
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 2 cut(s) 179, 479
MmeI TCCRAC 3 cut(s) 185, 219, 343
MnlI CCTC 7 cut(s) 308, 398, 526, 577, 785, 859, 909
Mox20I TGGCCA 1 cut(s) 117
MscI TGGCCA 1 cut(s) 117
MseI TTAA 5 cut(s) 38, 42, 369, 591, 623
MslI CAYNNNNRTG 3 cut(s) 102, 413, 860
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 3 cut(s) 260, 273, 411
MspR9I CCNGG 5 cut(s) 260, 261, 563, 705, 888
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 3 cut(s) 563, 705, 888
MvnI CGCG 2 cut(s) 294, 642
NciI CCSGG 2 cut(s) 260, 261
NcoI CCATGG 2 cut(s) 103, 112
NdeI CATATG 2 cut(s) 400, 977
NdeII GATC 2 cut(s) 528, 838
NlaIII CATG 5 cut(s) 107, 116, 448, 859, 924
NlaIV GGNNCC 2 cut(s) 409, 438
NmuCI GTSAC 3 cut(s) 107, 220, 980
OliI CACNNNNGTG 1 cut(s) 413
PacI TTAATTAA 1 cut(s) 42
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 3 cut(s) 11, 249, 812
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 886
PinAI ACCGGT 1 cut(s) 410
PkrI GCNGC 4 cut(s) 293, 436, 710, 786
PleI GAGTC 2 cut(s) 179, 479
PpsI GAGTC 2 cut(s) 179, 479
Psp6I CCWGG 3 cut(s) 561, 703, 886
PspEI GGTNACC 1 cut(s) 107
PspGI CCWGG 3 cut(s) 561, 703, 886
PspN4I GGNNCC 2 cut(s) 409, 438
PspPI GGNCC 1 cut(s) 565
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 3 cut(s) 191, 971, 1082
RsaNI GTAC 3 cut(s) 190, 970, 1081
RseI CAYNNNNRTG 3 cut(s) 102, 413, 860
SaqAI TTAA 5 cut(s) 38, 42, 369, 591, 623
SatI GCNGC 4 cut(s) 292, 435, 709, 785
Sau3AI GATC 2 cut(s) 528, 838
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 2 cut(s) 179, 479
ScrFI CCNGG 5 cut(s) 260, 261, 563, 705, 888
SduI GDGCHC 1 cut(s) 1018
SfcI CTRYAG 2 cut(s) 6, 792
SgrAI CRCCGGYG 1 cut(s) 410
SmaI CCCGGG 1 cut(s) 261
SmiMI CAYNNNNRTG 3 cut(s) 102, 413, 860
SsiI CCGC 2 cut(s) 292, 434
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 1084
StyD4I CCNGG 5 cut(s) 258, 259, 561, 703, 886
StyI CCWWGG 3 cut(s) 103, 112, 426
TaaI ACNGT 2 cut(s) 171, 1013
TaqI TCGA 5 cut(s) 150, 459, 815, 837, 948
TatI WGTACW 2 cut(s) 969, 1080
TauI GCSGC 2 cut(s) 294, 437
TfiI GAWTC 3 cut(s) 11, 249, 812
Tru1I TTAA 5 cut(s) 38, 42, 369, 591, 623
Tru9I TTAA 5 cut(s) 38, 42, 369, 591, 623
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 3 cut(s) 107, 220, 980
TseI GCWGC 2 cut(s) 708, 784
Tsp45I GTSAC 3 cut(s) 107, 220, 980
TspDTI ATGAA 7 cut(s) 23, 404, 466, 589, 817, 825, 844
TspGWI ACGGA 5 cut(s) 261, 399, 478, 508, 850
TspMI CCCGGG 1 cut(s) 259
TspRI CASTG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 231
XapI RAATTY 4 cut(s) 44, 350, 391, 719
XcmI CCANNNNNNNNNTGG 2 cut(s) 110, 737
XmaI CCCGGG 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.