FvH4_6g18290

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001140
Physical Location & Seq
Reverse (-)
12087122 .. 12093248
6127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18290.t1

Sequence Viewer

Length: 1086 bp
ATGGAGTTGGCTCAGACCCTCTTCCTCCCTCTCCTTCTATGTCTTCTCTTTCTTCTAACACAACAAAGTGCGTCACTGGTTTCAGCTGGCCACCACCACCACCACCACCACCATCCAACACATAGACACTTGTTCGATTGGCGTCCAACCAAACTGTTTGTATTCGGAGACTCGTACTCCGACACCGGCAACAACATAAAGTCAGTGACTAAATCCTGGAAATACCCTTACGGAATCACCTTCCCGGGAAGACCCACCGGCCGTTTTTCCGATGGCCGCGTCCTCACTGACTACCTTGCTAAGTTTGTAGGGGTGAGGTCTCCAATACCATACAAATTTAGAAATGTCGGAGTCAAACATTTGAAATACGGAATGAATTTCGCATATGGAGGCACTGGTGTGTTCAATACTTTGGTTGCGGCACCAAACATGACCACCCAAATCGATTTTTTTCAGCAATTCATCAAAGATTCTGTGTTCAATGCCCAAGATCTACACTCCTCCGTGGCATTGATCACTCTTGCAGGCAATGACTACGCATACTATCTGGCCCTGAATAATTCTTCTGAGGGTTTTAAACCTTTCATCACATCAGTTGTAAATCAACTAAGTGTGAACTTGAAACGTGTGTATGACTTGGGAGTGAAGAAAATAGTTGTGACAGCTCTACAACCTTTGGGATGTCTTCCTGGTAGCACAGCCGAATTTTCATTCCAGAAGTGCAATGGAACACAAAACTTGCTGGTCGGCTTCCACAACCTCTTGTTGCAGCAAGCTGTTGCCAAGTTGAACAACGAAACAAAGAGTTCTTTTTTCGTTCTTGATCTATATACCTCATTCATGTCCGTGCTCAAGAATAATGGAAACCATCTAGGAAGCATAAAGTTTGAGAATCCATTGAAGCCATGCTGTGTTGGTATAAGCAGCAAATATGATTGTGGAAGTGTCGATGACAGCGGCGCAAAGAAGTACACCATATGTGACGACCCTGCATCTGCATTCTTTTGGGACGGTGCTCACCCTACTCAACAGGGTTGGCTTGCTGTGTATTCAGCTCTGCAAGCCACACTTGAAGAATTGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

40.15

Weight (kDa)

8.81

Isoelectric Point (pI)

25.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 52 - 350 2.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 421
AccII CGCG 1 cut(s) 279
AciI CCGC 3 cut(s) 277, 419, 957
AcoI YGGCCR 3 cut(s) 88, 259, 274
AcsI RAATTY 3 cut(s) 335, 376, 704
AcyI GRCGYC 1 cut(s) 142
AfaI GTAC 3 cut(s) 176, 971, 1082
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 7 cut(s) 364, 406, 481, 622, 790, 901, 1073
AjnI CCWGG 2 cut(s) 215, 688
AleI CACNNNNGTG 1 cut(s) 398
AluBI AGCT 4 cut(s) 86, 665, 776, 1055
AluI AGCT 4 cut(s) 86, 665, 776, 1055
Alw21I GWGCWC 2 cut(s) 852, 1018
Alw26I GTCTC 2 cut(s) 162, 324
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 4 cut(s) 88, 259, 274, 549
ApeKI GCWGC 2 cut(s) 769, 924
ApoI RAATTY 3 cut(s) 335, 376, 704
AspLEI GCGC 1 cut(s) 962
AspS9I GGNCC 1 cut(s) 550
AsuC2I CCSGG 2 cut(s) 245, 246
AsuHPI GGTGA 3 cut(s) 229, 325, 1010
AvaI CYCGRG 1 cut(s) 244
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 421
BbsI GAAGAC 3 cut(s) 35, 256, 677
Bbv12I GWGCWC 2 cut(s) 852, 1018
BbvI GCAGC 2 cut(s) 781, 936
BccI CCATC 3 cut(s) 120, 266, 876
BceAI ACGGC 1 cut(s) 246
BciT130I CCWGG 2 cut(s) 217, 690
BclI TGATCA 1 cut(s) 513
BcnI CCSGG 2 cut(s) 245, 246
BcoDI GTCTC 2 cut(s) 162, 324
BfaI CTAG 2 cut(s) 872, 1084
BglII AGATCT 1 cut(s) 490
BisI GCNGC 5 cut(s) 277, 420, 770, 925, 958
BlsI GCNGC 5 cut(s) 278, 421, 771, 926, 959
Bme1390I CCNGG 4 cut(s) 217, 245, 246, 690
BmeT110I CYCGRG 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 4 cut(s) 217, 245, 246, 690
BmsI GCATC 1 cut(s) 1001
BpiI GAAGAC 3 cut(s) 35, 256, 677
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 2 cut(s) 245, 246
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 244, 504
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bse118I RCCGGY 2 cut(s) 185, 257
Bse1I ACTGG 2 cut(s) 81, 400
Bse3DI GCAATG 2 cut(s) 535, 730
BseBI CCWGG 2 cut(s) 217, 690
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 244, 504
BseGI GGATG 2 cut(s) 112, 686
BseMI GCAATG 2 cut(s) 535, 730
BseMII CTCAG 2 cut(s) 26, 558
BseNI ACTGG 2 cut(s) 81, 400
BseRI GAGGAG 1 cut(s) 490
BseX3I CGGCCG 1 cut(s) 259
BseXI GCAGC 2 cut(s) 781, 936
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 4 cut(s) 90, 261, 276, 551
BshNI GGYRCC 1 cut(s) 421
BshVI ATCGAT 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 2 cut(s) 852, 1018
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 3 cut(s) 186, 245, 258
BslFI GGGAC 1 cut(s) 1022
BsmAI GTCTC 2 cut(s) 162, 324
BsmFI GGGAC 1 cut(s) 1022
BsmI GAATGC 1 cut(s) 998
BsnI GGCC 4 cut(s) 90, 261, 276, 551
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 852, 1018
Bsp143I GATC 3 cut(s) 490, 513, 823
BspACI CCGC 3 cut(s) 277, 419, 957
BspANI GGCC 4 cut(s) 90, 261, 276, 551
BspCNI CTCAG 2 cut(s) 25, 559
BspDI ATCGAT 1 cut(s) 444
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 324
BsrDI GCAATG 2 cut(s) 535, 730
BsrFI RCCGGY 2 cut(s) 185, 257
BsrI ACTGG 2 cut(s) 81, 400
BssAI RCCGGY 2 cut(s) 185, 257
BssECI CCNNGG 2 cut(s) 244, 504
BssMI GATC 3 cut(s) 490, 513, 823
BssNI GRCGYC 1 cut(s) 142
Bst2UI CCWGG 2 cut(s) 217, 690
Bst4CI ACNGT 2 cut(s) 156, 1013
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 142
BstC8I GCNNGC 5 cut(s) 88, 526, 774, 1041, 1062
BstDEI CTNAG 4 cut(s) 12, 300, 567, 608
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 112, 686
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 962
BstKTI GATC 3 cut(s) 493, 516, 826
BstMAI GTCTC 2 cut(s) 162, 324
BstMBI GATC 3 cut(s) 490, 513, 823
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 1061
BstNI CCWGG 2 cut(s) 217, 690
BstSCI CCNGG 4 cut(s) 215, 243, 244, 688
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 781, 936
BstV2I GAAGAC 3 cut(s) 35, 256, 677
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BstZI CGGCCG 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 4 cut(s) 90, 261, 276, 551
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 2 cut(s) 112, 686
BtsIMutI CAGTG 4 cut(s) 74, 210, 285, 393
Cac8I GCNNGC 5 cut(s) 88, 526, 774, 1041, 1062
CfoI GCGC 1 cut(s) 962
Cfr10I RCCGGY 2 cut(s) 185, 257
Cfr13I GGNCC 1 cut(s) 550
Cfr9I CCCGGG 1 cut(s) 244
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 3 cut(s) 60, 131, 268
Csp6I GTAC 3 cut(s) 175, 970, 1081
CviAII CATG 3 cut(s) 430, 841, 906
CviQI GTAC 3 cut(s) 175, 970, 1081
DdeI CTNAG 4 cut(s) 12, 300, 567, 608
DpnI GATC 3 cut(s) 492, 515, 825
DpnII GATC 3 cut(s) 490, 513, 823
DraI TTTAAA 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 88, 259, 274
EagI CGGCCG 1 cut(s) 259
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 324
Eco52I CGGCCG 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 215, 688
FaeI CATG 3 cut(s) 433, 844, 909
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 1 cut(s) 1022
FatI CATG 3 cut(s) 429, 840, 905
FauNDI CATATG 2 cut(s) 385, 977
FbaI TGATCA 1 cut(s) 513
Fnu4HI GCNGC 5 cut(s) 277, 420, 770, 925, 958
FokI GGATG 2 cut(s) 99, 693
Fsp4HI GCNGC 5 cut(s) 277, 420, 770, 925, 958
FspBI CTAG 2 cut(s) 872, 1084
GlaI GCGC 1 cut(s) 961
GluI GCNGC 5 cut(s) 277, 420, 770, 925, 958
HaeIII GGCC 4 cut(s) 90, 261, 276, 551
HapII CCGG 3 cut(s) 186, 245, 258
HgaI GACGC 3 cut(s) 60, 131, 268
HhaI GCGC 1 cut(s) 962
Hin1I GRCGYC 1 cut(s) 142
Hin1II CATG 3 cut(s) 433, 844, 909
Hin6I GCGC 1 cut(s) 960
HinP1I GCGC 1 cut(s) 960
HinfI GANTC 5 cut(s) 170, 234, 351, 470, 892
HpaII CCGG 3 cut(s) 186, 245, 258
HphI GGTGA 3 cut(s) 229, 325, 1010
Hpy166II GTNNAC 2 cut(s) 616, 972
Hpy188I TCNGA 6 cut(s) 15, 167, 181, 271, 350, 568
Hpy188III TCNNGA 3 cut(s) 715, 821, 853
Hpy8I GTNNAC 2 cut(s) 616, 972
HpyAV CCTTC 2 cut(s) 44, 250
HpyCH4III ACNGT 2 cut(s) 156, 1013
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 6 cut(s) 524, 723, 769, 992, 998, 1060
HpyF10VI GCNNNNNNNGC 1 cut(s) 1061
HpyF3I CTNAG 4 cut(s) 12, 300, 567, 608
HpySE526I ACGT 1 cut(s) 625
Hsp92I GRCGYC 1 cut(s) 142
Hsp92II CATG 3 cut(s) 433, 844, 909
HspAI GCGC 1 cut(s) 960
Ksp22I TGATCA 1 cut(s) 513
Kzo9I GATC 3 cut(s) 490, 513, 823
Lsp1109I GCAGC 2 cut(s) 781, 936
LweI GCATC 1 cut(s) 1001
MaeI CTAG 2 cut(s) 872, 1084
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 4 cut(s) 72, 205, 658, 980
MalI GATC 3 cut(s) 492, 515, 825
MboI GATC 3 cut(s) 490, 513, 823
MboII GAAGA 8 cut(s) 13, 35, 44, 261, 555, 658, 677, 1085
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 2 cut(s) 852, 1018
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 6 cut(s) 335, 376, 458, 559, 704, 1076
MluNI TGGCCA 1 cut(s) 90
MlyI GAGTC 2 cut(s) 164, 360
MmeI TCCRAC 4 cut(s) 140, 170, 204, 328
Mox20I TGGCCA 1 cut(s) 90
MscI TGGCCA 1 cut(s) 90
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 2 cut(s) 398, 845
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 2 cut(s) 86, 957
MspI CCGG 3 cut(s) 186, 245, 258
MspR9I CCNGG 4 cut(s) 217, 245, 246, 690
Mva1269I GAATGC 1 cut(s) 998
MvaI CCWGG 2 cut(s) 217, 690
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 1061
NciI CCSGG 2 cut(s) 245, 246
NdeI CATATG 2 cut(s) 385, 977
NdeII GATC 3 cut(s) 490, 513, 823
NlaIII CATG 3 cut(s) 433, 844, 909
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 4 cut(s) 72, 205, 658, 980
OliI CACNNNNGTG 1 cut(s) 398
PctI GAATGC 1 cut(s) 998
PfeI GAWTC 3 cut(s) 234, 470, 892
PfoI TCCNGGA 1 cut(s) 215
PkrI GCNGC 5 cut(s) 278, 421, 771, 926, 959
PleI GAGTC 2 cut(s) 164, 359
PpsI GAGTC 2 cut(s) 164, 359
Psp6I CCWGG 2 cut(s) 215, 688
PspGI CCWGG 2 cut(s) 215, 688
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 550
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 3 cut(s) 176, 971, 1082
RsaNI GTAC 3 cut(s) 175, 970, 1081
RseI CAYNNNNRTG 2 cut(s) 398, 845
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 5 cut(s) 277, 420, 770, 925, 958
Sau3AI GATC 3 cut(s) 490, 513, 823
Sau96I GGNCC 1 cut(s) 550
SchI GAGTC 2 cut(s) 164, 360
ScrFI CCNGG 4 cut(s) 217, 245, 246, 690
SduI GDGCHC 2 cut(s) 852, 1018
SfaNI GCATC 1 cut(s) 1001
SmaI CCCGGG 1 cut(s) 246
SmiMI CAYNNNNRTG 2 cut(s) 398, 845
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 6 cut(s) 335, 376, 458, 559, 704, 1076
SsiI CCGC 3 cut(s) 277, 419, 957
SspMI CTAG 2 cut(s) 872, 1084
StyD4I CCNGG 4 cut(s) 215, 243, 244, 688
TaaI ACNGT 2 cut(s) 156, 1013
TaiI ACGT 1 cut(s) 628
TaqI TCGA 3 cut(s) 135, 444, 948
TasI AATT 6 cut(s) 335, 376, 458, 559, 704, 1076
TatI WGTACW 2 cut(s) 969, 1080
TauI GCSGC 3 cut(s) 279, 422, 960
TfiI GAWTC 3 cut(s) 234, 470, 892
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 4 cut(s) 81, 210, 292, 400
TseFI GTSAC 4 cut(s) 72, 205, 658, 980
TseI GCWGC 2 cut(s) 769, 924
Tsp45I GTSAC 4 cut(s) 72, 205, 658, 980
TspDTI ATGAA 5 cut(s) 389, 451, 574, 699, 829
TspGWI ACGGA 4 cut(s) 246, 384, 493, 835
TspMI CCCGGG 1 cut(s) 244
TspRI CASTG 4 cut(s) 81, 210, 292, 400
XapI RAATTY 3 cut(s) 335, 376, 704
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmaI CCCGGG 1 cut(s) 244
XspI CTAG 2 cut(s) 872, 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.